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Identification and analysis of differentially expressed microRNAs in gibel carp carassius auratus gibelio responding to polyinosinic-polycytidylic acid (poly I:c) stimulation

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FISH AND SHELLFISH IMMUNOLOGY REPORTS 2023年 4卷

作者： Zhang, Jialin Sun, Shanling Mao, Yiyang Qiao, Guo Li, Qiang Yancheng Inst Technol Coll Marine & Bioengn Yancheng 224051 Peoples R China

Polyinosinic-polycytidylic acid (poly I:c) is a synthesized analogue of viral double-strand RNA and considered as a potential immunostimulant in aquaculture. MicroRNAs (miRNAs) have been reported to play important roles in the development of the immune system and in regulation of host antiviral responses. In our earlier study, it was found that poly I:c pre-treatment could stimulate the resistance against cyprinid herpesvirus 2 (cyHV-2) infection and enhance the antiviral immune response in gibel carp. To understand the role of miRNAs in regulating the host response to poly I:c treatment, we investigated the expression profiles of miRNAs in the head kidney of poly I:c-treated gibel carp with small RNA sequencing technology. When compared with the untreated group, a total of 24 differentially expressed miRNAs were identified in the poly I:c-stimulated fish, among which, 7 and 17 miRNAs were upregulated and downregulated, respectively. Analysis of target genes of these differentially expressed miRNAs found that most targeted mRNAs were involved in catalytic activity, peptidase activity and endopeptidase activity, and were enriched in the metabolic, protein processing in endoplasmic reticulum and oxidative phosphorylation signaling pathways, suggesting that poly I:c could alter the expression of metabolism-related miRNAs in the kidney of gibel carp. Besides, it was noted that some immune-related miRNAs, including inflammation-related miRNAs (miR-192 and miR-731) and interferon-related miRNAs (miR-194a and miR-122), were downregulated after poly I:c treatment. In summary, it was found that poly I:c could regulate the cellular levels of specific miRNAs involved in metabolism and immune responses in the head kidney of gibel carp, which may increase the capacity of the immune cells to fight against pathogens infection.

关键词： Gibel carp Poly I c MicroRNA Transcriptome Immune response

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seco-Prezizanne Sesquiterpenes and Prenylated c6-c3 compounds from the Fruits of Illicium lanceolatum A. c. Smith

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cHEMISTRY & BIODIVERSITY 2022年第1期19卷 e202100868-e202100868页

作者： Nie, Wei Ding, Lin-Fen Tie-Lei Zhou, Hao-Feng Bao, Yi Song, Liu-Dong Pan, Zheng-Hong Wu, Xing-De Zhao, Qin-Shi Chinese Acad Sci Kunming Inst Bot State Key Lab Phytochem & Plant Resources West Ch Kunming 650201 Yunnan Peoples R China Yunnan Minzu Univ State Ethn Affairs Commiss Key Lab Ethn Med Resource Chem Kunming 650500 Yunnan Peoples R China Yunnan Minzu Univ Minist Educ Kunming 650500 Yunnan Peoples R China Kunming Med Univ Sch Pharmaceut Sci Kunming 650500 Yunnan Peoples R China Kunming Med Univ Yunnan Key Lab Pharmacol Nat Prod Kunming 650500 Yunnan Peoples R China Chinese Acad Sci Guangxi Inst Bot Guangxi Key Lab Funct Phytochem Res & Utilizat Guilin 541006 Peoples R China

Two new seco-prezizaane-type sesquiterpenes, 2 beta-hydroxy-6-deoxyneoanisatin (1) and 3,4-anhydro-2-oxo-1 alpha-hydroxy-6-deoxyneoanisatin (2), and two new prenylated c-6-c-3 compounds, illilanceofunones A (3) and B (4), were obtained from the fruits of Illicium lanceolatum, along with four known prenylated c-6-c-3 compounds (5-8). Their structures were proposed through HR-ESI-MS, H-1, c-13, and 2D NMR data interpretation. Moreover, the absolute configuration of 1 and 2 were further assigned by single-crystal X-ray diffraction analysis and electronic circular dichroism (EcD) calculations, respectively. Illihenryipyranol A (6) exhibited neuroprotective activity against MPP+-induced Pc12 cell damage in a dose-dependent manner.

关键词： Illicium lanceolatum A c Smith seco-prezizanne sesquiterpene prenylated c6-c3 compound neuroprotective activity

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PITX2c increases the stemness features of hepatocellular carcinoma cells by up-regulating key developmental factors in liver progenitor

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JOURNAL OF EXPERIMENTAL & cLINIcAL cANcER RESEARcH 2022年第1期41卷 211-211页

作者： Jiang, Lingxi Wang, Xia Ma, Fangfang Wang, Xuelong Shi, Minmin Yan, Qian Liu, Ming chen, Juan Shi, chaoran Guan, Xin-yuan Shanghai Jiao Tong Univ Ruijin Hosp Dept Gen Surg Sch Med Shanghai 200025 Peoples R China Univ Hong Kong Li Ka Shing Fac Med Dept Clin Oncol Pokfulam Room L10-56Lab Block21 Sassoon Rd Hong Kong Peoples R China Shanghai Jiao Tong Univ Res Inst Pancreat Dis Sch Med Shanghai Peoples R China Univ Hong Kong State Key Lab Liver Res Hong Kong Peoples R China Sun Yat Sen Univ Guangdong Inst Gastroenterol Affiliated Hosp 6 Dept Colorectal SurgGuangdong Prov Key Lab Color Guangzhou Peoples R China Guangzhou Med Univ Sch Basic Med Sci Affiliated Canc Hosp & Inst Guangzhou Municipal & Guangdong Prov Key Lab Prot Guangzhou Peoples R China

Background Tumor cells exhibited phenotypic and molecular characteristics similar to their lineage progenitor cells. Liver developmental signaling pathways are showed to be associated with Hcc development and oncogenesis. The similarities of expression profiling between liver progenitors (LPs) and Hcc suggest that understanding the molecular mechanism during liver development could provide insights into Hcc. Methods To profile the dynamic gene expression during liver development, cells from an in vitro liver differentiation model and two paired hepatocellular carcinoma (Hcc) samples were analyzed using deep RNA sequencing. The expression levels of selected genes were analyzed by qRT-PcR. Moreover, the role of a key transcription factor, pituitary homeobox 2 (PITX2), was characterized via in vitro and vivo functional assays. Furthermore, molecular mechanism studies were performed to unveil how PITX2c regulate the key developmental factors in LPs, thereby increasing the stemness of Hcc. Results PITX2 was found to exhibit a similar expression pattern to specific markers of LPs. PITX2 consists of three isoforms (PITX2A/B/c). The expression of PITX2 is associated with tumor size and overall survival rate, whereas only PITX2c expression is associated with AFP and differentiation in clinical patients. PITX2A/B/c has distinct functions in Hcc tumorigenicity. PITX2c promotes Hcc metastasis, self-renewal and chemoresistance. Molecular mechanism studies showed that PITX2c could up-regulate RALYL which could enhance Hcc stemness via the TGF-beta pathway. Furthermore, chIP assays confirmed the role of PITX2c in regulating key developmental factors in LP. conclusion PITX2c is a newly discovered transcription factor involved in hepatic differentiation and could increase Hcc stemness by upregulating key transcriptional factors related to liver development.

关键词： PITX2A B c Hcc Tumorigenicity Stemness Developmental factors in liver progenitor

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Sperm associated antigen 4 promotes SREBP1-mediated de novo lipogenesis via interaction with lamin A/c and contributes to tumor progression in hepatocellular carcinoma

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cANcER LETTERS 2022年 536卷 215642-215642页

作者： Liu, Tengfei Yu, Junming Ge, chao Zhao, Fangyu chen, Jing Miao, chunxiao Jin, Wenjiao Zhou, Qingqing Geng, Qin Lin, Hechun Tian, Hua chen, Taoyang Xie, Haiyang cui, Ying Yao, Ming Xiao, Xiuying Li, Jinjun Li, Hong Shanghai Jiao Tong Univ Renji Hosp Shanghai Canc Inst State Key Lab Oncogenes & Related GenesSch Med Shanghai 200032 Peoples R China Shanghai Jiao Tong Univ Rui jin Hosp Sch Med Dept Oncol Shanghai 200020 Peoples R China Qi Dong Liver Canc Inst Qi Dong 226200 Peoples R China Zhejiang Univ Affiliated Hosp 1 Sch Med Dept Gen Surg Hangzhou 310000 Peoples R China Canc Inst Guangxi Nanning 530027 Peoples R China Shanghai Jiao Tong Univ Ren ji Hosp Sch Med Dept Oncol Shanghai 200127 Peoples R China

Hepatocellular carcinoma (Hcc) is a highly malignant tumor and its progression is associated with altered lipid metabolism in precancerous lesions, such as non-alcoholic fatty liver disease. Here, we identified sperm associated antigen 4 (SPAG4), and explored its oncogenic role in Hcc progression. Database analysis and immunohistochemistry indicated increased level of SPAG4 in Hcc tissues which was of prognostic value. Gain/loss-offunction experiments showed that SPAG4 exerted oncogenic roles in Hcc growth both in vitro and in vivo. RNA sequencing revealed activation of a lipogenic state and SREBP1-mediated pathway following SPAG4 overexpression. Mechanistically, the N-terminal region of SPAG4 bound to lamin A/c, which increased SREBP1 expression, nuclear translocation, and transcriptional activity. Treatment with orlistat, a lipid synthesis inhibitor, reversed SPAG4-mediated oncogenic effects, and its efficacy varied with SPAG4 level. The effect of orlistat was further amplified when combined with sorafenib in tumor xenograft mouse models. Our study provides evidence that SPAG4 mediates Hcc progression by affecting lipid metabolism. Administration of orlistat combined with sorafenib reverses SPAG4-mediated oncogenesis in Hcc cells and ectopic xenograft tumors in mice, suggesting that this pathway represents a potential target for Hcc treatment.

关键词： Hepatocellular carcinoma SPAG4 Lipogenesis SREBP1 Lamin A c

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Performance and microbial characterization of aerobic granular sludge in a sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal with varying c/N ratios

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BIOPROcESS AND BIOSYSTEMS ENGINEERING 2020年第4期43卷 663-672页

作者： Zhou, Jun Sun, Qianyu Nanjing Tech Univ Coll Urban Construct Nanjing 211800 Peoples R China

The mechanism and effect of c/N ratios on the aerobic granules simultaneous nitrification, denitrification and phosphorus removal (SNDPR) system are still unclear. The reactor performance and microbial community dynamics of the system were investigated under variable c/N ratios (20, 10 and 5). The cOD, TP and NH4+-N removal remained unaffected with variable c/N ratios. The decreased c/N ratio of five strongly influenced the nitrogen removal. Further investigations revealed that candidatus_Accumulibacter, Acinetobacter, candidatus_competibacter were the predominant genera. classification of key groups involved in nitrogen and phosphorus removal indicated the lowest c/N ratio resulting in a large microbial community shift. This study might contribute to the application of SNDPR system for the treatment of wastewater. Different c/N ratios led to shift on the microbial community and the dominant was phosphorus-accumulating bacteria. The nitrogen removal efficiency decreased while the removal of cOD, TP and NH4+-N remained remarkable with the decreased c/N ratios.

关键词： Aerobic granular sludge c N ratio Functional species Microbial community analysis SNDPR

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Modeling Temperature-Dependent Vibration Damping in c/Sic Fiber-Reinforced ceramic-Matrix composites

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MATERIALS 2020年第7期13卷 1633页

作者： Li, Longbiao Nanjing Univ Aeronaut & Astronaut Coll Civil Aviat 29 Yudao St Nanjing 210016 Peoples R China

In this paper, the temperature-dependent vibration damping in c/Sic fiber-reinforced ceramic-matrix composites (cMcs) with different fiber preforms under different vibration frequencies is investigated. A micromechanical temperature-dependent vibration damping model is developed to establish the relationship between composite damping, material properties, internal damage mechanisms, and temperature. The effects of fiber volume, matrix crack spacing, and interface properties on temperature-dependent composite vibration damping of cMcs and interface damage are analyzed. The experimental temperature-dependent composite damping of 2D and 3D c/Sic composites is predicted for different loading frequencies. The damping of the c/Sic composite increases with temperature to the peak value and then decreases with temperature. When the vibration frequency increases from f = 1 to 10 Hz, the peak value of composite damping and corresponding temperature both decrease due to the decrease of interface debonding and slip range, and the damping of 2D c/Sic is much higher than that of 3D c/Sic at temperature range from room temperature to 400 degrees c. When the fiber volume and interface debonding energy increase, the peak value of composite damping and the corresponding temperature decreases, mainly attributed to the decrease of interface debonding and slip range.

关键词： ceramic-matrix composites (cMcs) c Sic damping temperature-dependent matrix cracking interface debonding

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Obtaining c2 and c3 Products from Methane Using Pd/c as Anode in a Solid Fuel cell-type Electrolyte Reactor

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cHEMcATcHEM 2020年第18期12卷 4517-4521页

作者： Ramos, Andrezza S. Santos, Monique carolina L. Godoi, camila M. Oliveira Neto, Almir Fernando B. De Souza, Rodrigo IPEN CNEN SP Inst Pesquisas Energet & Nucl Ctr Celula Combustivel & Hidrogenio Ave Prof Lineu Prestes2242 Cidade Univ BR-05508000 Sao Paulo SP Brazil

Methane was converted into c2 and c3 products under mild conditions using a single stage solid electrolyte reactor, using a proton exchange membrane fuel cell as a SER-Fc and Pd/c as an electrocatalyst prepared by the reduction method of sodium borohydride. This electrocatalyst has a cubic pattern of palladium centered on the face and an average size of nanoparticles close to 6.4 nm, according to the literature. Differential mass spectrometry reveals the chemical profile of species obtained from the oxidation of methane with ionic currents (Ii) at m/z=16, 28, 30, 32, 44, 46 and 60. In many cases, Ii can be assigned to more than one species;therefore, complementary ATR-FTIR experiments were performed. The ATR-FTIR spectra confirmed the presence of c2 and c3 compounds such as ethane, ethanol, acetaldehyde, acetic acid and propane. considering the low amount of water in the reaction medium, these results may be associated with the use of Pd/c electrocatalysts responsible for the activation of the water molecule.

关键词： OcM reaction methane oxidation Pd c Natural gas to products

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Space Diversity Mitigation Effects on Ionospheric Amplitude Scintillation With Basis on the Analysis of c/NOFS Planar Langmuir Probe Data

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RADIO ScIENcE 2020年第12期55卷 1-11页

作者： costa, Emanoel Roddy, Patrick A. Ballenthin, John O. Pontificia Univ Catolica Rio de Janeiro PUC Rio Ctr Estudos Telecomunicacoes CETUC Rio De Janeiro Brazil Air Force Res Lab Space Vehicles Directorate Kirtland Air Force Base NM USA

The present contribution applies a modified version of a well-known weak-scatter scintillation model to the propagation paths between an Earth station and two geostationary satellites, as they are affected by drifting ionospheric irregularities detected by the planar Langmuir probe onboard the communications/Navigation Outage Forecasting System (c/NOFS) satellite. This study considers one year of data from a limited altitude range, two longitude sectors and frequencies to analyze the mitigation effects from the angular distance between the geostationary satellites on the space diversity gain, considering the statistical distributions of the amplitude scintillation index S-4. The results indicate that the space diversity gain increases with the angular distance between geostationary satellites. However, once the angular distance between the geostationary satellites reaches a certain limit (approximately 20 degrees), the space diversity gain no longer increases. Additionally, these gains are high at the L1 frequency and less impressive at an Ultra High Frequency (UHF). It is shown that these observations are consistent with the South American and Indian-Asian equatorial and low-latitude ionospheric environments, also characterized through the same data and the scintillation formulation.

关键词： ionospheric scintillation space diversity c NOFS PLP

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clog: A Declarative Language for c Static code checkers 2024

Clog: A Declarative Language for C Static Code Checkers

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33rd AcM SIGPLAN International conference on compiler construction (cc)

作者： Dura, Alexandru Reichenbach, christoph Lund Univ Lund Sweden

ISBN: (纸本)9798400705076

We present clog, a declarative language for describing static code checkers for c. Unlike other extensible state-of-the-art checker frameworks, clog enables powerful interprocedural checkers without exposing the underlying program representation: clog checkers consist of Datalog-style recursive rules that access the program under analysis via syntactic pattern matching and control flow edges only. We have implemented clog on top of clang, using a custom Datalog evaluation strategy that piggy-backs on clang's AST matching facilities while working around clang's limitations to achieve our design goal of representation independence. Our experiments demonstrate that clog can concisely express a wide variety of checkers for different security vulnerabilities, with performance that is similar to clang's own analyses and highly competitive on real-world programs.

关键词： Datalog c Syntactic Patterns Static Analysis Frameworks

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Biomass carbon-modified coZn/c as a high-performance electrode for supercapacitors

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IONIcS 2020年第10期26卷 5179-5188页

作者： Zhang, chunyong Huang, Linna Zhao, Yuyue Su, Lei Li, Jianning Qin, Hengfei Jiangsu Univ Technol Jiangsu Key Lab Precious Met Chem & Technol Changzhou 213001 Jiangsu Peoples R China

In this study, biomass carbon-modified coZn-ZIF-8 was firstly fabricated through a hybridization approach. The coZn/c was prepared by directly carbonizing a coZn-ZIF-8/c at the designed temperature and investigated by multiple techniques including XRD, XPS, SEM, and TEM analyses. After carbonization, coZn/c shows a high capacitance of 757.8 F/g at 5 mV/s, which is an outstanding characteristic compared with that of recently reported supercapacitor. As a consequence, the improved electrochemical performance of coZn/c attributed to the biomass carbon can significantly enhance the mass transport channels of electron-hole pairs. Remarkably, the composite shows excellent capacitive performances originating from the abundant reservoir for the charge storage derived from ZIF-derived porous and electroactive species exposure as well as high ion diffusion rate. coZn/c can be regarded as a promising material for practical supercapacitor, and this work also provides a valuable reference for the preparation and application of high-performance electrode materials which greatly promote the supercapacitor development.

关键词： Biomass carbon Supercapacitor coZn c Hybridization

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