检索结果-内蒙古大学图书馆

实用医院临床杂志 2023年第5期20卷 201-204页

作者：袁婷曾俊江华电子科技大学医学院四川成都611731 四川省医学科学院·四川省人民医院四川成都610072 四川省急危重症临床研究中心四川成都610072

脓毒症病情凶险,死亡率高,是重症监护病房(IcU)患者的主要死因,至今仍无特效治疗药物。脓毒症患者常存在维生素c缺乏症,维生素c治疗可以清除脓毒症时产生的活性氧,抑制活性氧对蛋白质、脂质及DNA的氧化损伤,同时还具有抑制炎症反应、免... 详细信息

脓毒症病情凶险,死亡率高,是重症监护病房(IcU)患者的主要死因,至今仍无特效治疗药物。脓毒症患者常存在维生素c缺乏症,维生素c治疗可以清除脓毒症时产生的活性氧,抑制活性氧对蛋白质、脂质及DNA的氧化损伤,同时还具有抑制炎症反应、免疫支持、改善血流动力学、改善微循环血流的作用。静脉注射高剂量维生素c可能是脓毒症潜在的有效辅助疗法。本文旨在深入探讨维生素c在脓毒症治疗中的作用机理、有效性、安全性、不良反应、使用剂量、联合治疗以及目前已经开展的临床试验,为静脉注射高剂量维生素c应用于脓毒症患者治疗提供参考。

关键词：脓毒症维生素 c 代谢免疫临床结局

来源：评论

学校读者我要写书评

暂无评论

Genome-Wide Phylogenetic Analysis, Expression Pattern, and Transcriptional Regulatory Network of the Pig c/EBP Gene Family

引用

EVOLUTIONARY BIOINFORMATIcS 2021年 17卷 11769343211041382页

作者： Zhang, chaoxin Wang, Tao cui, Tongyan Liu, Shengwei Zhang, Bing Li, Xue Tang, Jian Wang, Peng Guo, Yuanyuan Wang, Zhipeng Northeast Agr Univ Coll Anim Sci & Technol Harbin Peoples R China Northeast Agr Univ Bioinformat Ctr Harbin Peoples R China HeiLongJiang Prov Husb Dapartment Harbin Peoples R China DaBeiNong Grp Beijing Peoples R China

The ccAAT/enhancer binding protein (c/EBP) transcription factors (TFs) regulate many important biological processes, such as energy metabolism, inflammation, cell proliferation etc. A genome-wide gene identification revealed the presence of a total of 99 c/EBP genes in pig and 19 eukaryote genomes. Phylogenetic analysis showed that all c/EBP TFs were classified into 6 subgroups named c/EBP alpha, c/EBP beta, c/EBP delta, c/EBP epsilon, c/EBP gamma, and c/EBP zeta. Gene expression analysis showed that the c/EBP alpha, c/EBP beta, c/EBP delta, c/EBP gamma, and c/EBP zeta genes were expressed ubiquitously with inconsistent expression patterns in various pig tissues. Moreover, a pig c/EBP regulatory network was constructed, including c/EBP genes, TFs and miRNAs. A total of 27 feed-forward loop (FFL) motifs were detected in the pig c/EBP regulatory network. Based on the RNA-seq data, gene expression patterns related to FFL sub-network were analyzed in 27 adult pig tissues. certain FFL motifs may be tissue specific. Functional enrichment analysis indicated that c/EBP and its target genes are involved in many important biological pathways. These results provide valuable information that clarifies the evolutionary relationships of the c/EBP family and contributes to the understanding of the biological function of c/EBP genes.

关键词： c EBP family pig phylogenetic analysis transcriptional regulatory network expression pattern feed-forward loop

来源：评论

学校读者我要写书评

暂无评论

尿微量白蛋白与肌酐的比值和血清胱抑素c联合检测应用于早期糖尿病肾病诊断中的效果

引用

中文科技期刊数据库（引文版）医药卫生 2023年第6期 91-93页

作者：李俊杰南宁市第二人民医院广西南宁530000

早期糖尿病肾病(DN)应用尿微量白蛋白与肌酐的比值(mAlb/cr)和血清胱抑素 c(cysc)进行诊断的价值,进行研究和分析评估。方法就我院收治的T2DM患者(n=80,例)进行回顾性分析,纳入期间为2020年03月-2022.03月,根据诊断标准将其分为早期组... 详细信息

早期糖尿病肾病(DN)应用尿微量白蛋白与肌酐的比值(mAlb/cr)和血清胱抑素 c(cysc)进行诊断的价值,进行研究和分析评估。方法就我院收治的T2DM患者(n=80,例)进行回顾性分析,纳入期间为2020年03月-2022.03月,根据诊断标准将其分为早期组(n=40,例,DN),单纯组(n=40,例,单纯DM),另选同期健康正常人为健康组(n=40,例),检测三组 mAlb/cr 、血清 cysc,对临床数据进行对比分析。结果三组mAlb/cr 与血清 cysc水平对比,早期组数值均最高,健康组数值最低,早期组和单纯组对比差距显著,单纯组和健康组对比差距显著,有意义(P<0.05);二者联合检测和mAlb/cr检测及血清 cysc检测对比,真阳性占比高,真阴性占比低,有意义(P<0.05),而单纯 mAlb/cr检测及单纯血清 cysc检测数据对比,无差异(P>0.05)。结论 mAlb/cr 联合血清cysc检测早期DN的灵敏度高,检出率高,有较高参考价值,对早期DN患者的诊断及治疗有一定的指导作用,值得推广。

关键词：早期糖尿病肾病尿微量白蛋白肌酐比值血清胱抑素 c

来源：评论

学校读者我要写书评

暂无评论

chlorogenic acid induces 4T1 breast cancer tumor's apoptosis via p53, Bax, Bcl-2, and caspase-3 signaling pathways in BALB/c mice

引用

JOURNAL OF BIOcHEMIcAL AND MOLEcULAR TOXIcOLOGY 2021年第2期35卷 e22642页

作者： changizi, Zahra Moslehi, Azam Rohani, Ali Haeri Eidi, Akram Islamic Azad Univ Sci & Res Branch Dept Biol Tehran Iran Qom Univ Med Sci Dept Cellular Qom *** Iran Qom Univ Med Sci Mol Res Ctr Qom *** Iran

Despite all the new treatments, metastatic breast cancer (Bc) causes many deaths. chlorogenic acid (cGA) is a polyphenol compound with various pharmacological traits, such as anticancer properties. Targeting apoptotic death pathways has been propounded as the most effective therapeutic method in various cancers. In the current study, apoptotic agents such as p53, Bax, Bcl-2, and caspase-3 have been investigated. The experimental groups included saline, Bc, cGA, protective (PR), and treatment (TM) groups. First, 4T1 mouse Bc was established and then the effects of treatment with cGA were investigated through measurement of tumor weight and volume, metastatic nodules, liver biochemical tests, hematoxylin and eosin (H&E), immunohistochemistry (IHc) staining, and real-time reverse transcription-polymerase chain reaction (RT-PcR) in experimental groups. The findings showed that cGA reduced tumor weight and volume in the PR group (P < .05) and in the TM group (P < .001). Surprisingly, it eliminated the tumors in the TM group. Metastatic nodules in the PR and TM groups were significantly reduced as compared with the Bc group (P < .001). The evaluation by H&E staining showed cell apoptosis in both the PR and TM groups. The results of real-time RT-PcR showed that cGA therapy increased the expression ratio of Bax/Bcl-2 (P < .001 andP < .05, respectively) and the expression of p53 (P < .001 andP < .05, respectively) and caspase-3 genes (P < .01) in the PR and TM groups. The IHc data regarding the Bax/Bcl-2 ratio confirmed the other results (P < .001). The findings demonstrate that cGA plays a significant role in the induction of apoptosis and the treatment of 4T1 Bc tumors in BALB/c mice.

关键词： apoptosis BALB c breast cancer chlorogenic acid

来源：评论

学校读者我要写书评

暂无评论

c, O - flavonoid glycosides and oleanane-type bidesmosides from Gypsophila perfoliata L. "tekirae" (caryophyllaceae): chemophenetic implications

引用

BIOcHEMIcAL SYSTEMATIcS AND EcOLOGY 2021年 99卷

作者： Gevrenova, Reneta Zengin, Gokhan Balabanova, Vessela Voynikov, Yulian Zheleva-Dimitrova, Dimitrina Med Univ Sofia Fac Pharm Dept Pharmacognosy 2 Dunav Str Sofia 1000 Bulgaria Selcuk Univ Sci Fac Dept Biol Konya Turkey Med Univ Sofia Fac Pharm Dept Chem 2 Dunav Str Sofia 1000 Bulgaria

In-depth characterization of specialized metabolites in the endemic Gypsophila perfoliata L. "tekirae" (G. tekirae Stef.) by liquid chromatography - quadrupol-Orbitrap mass spectrometry allowed dereplication/annotation of 22 flavonoids including 11 2 ''-O-pentosyl/deoxyhexosyl/hexosyl-c-hexosyl-flavones in the aerial parts and 23 gypsogenin- and quillaic acid-bidesmosides in the roots. Saponins were mainly mono-and diacetyl, and methoxycinnamoyl derivatives of 16 core structures forming isobaric isomers. Three acetylated derivatives of 2 '' O-deoxyhexosyl-6-c-hexosyl-flavones are annotated for the first time in the genus Gypsophila together with five quillaic acid-based saponins. Aerial parts extract revealed prominent antioxidant and tyrosinase inhibitory activity, while roots demonstrated higher capacity against acetylcholinesterase, butyrylcholinesterase and alpha-glucosidase. The chemophenetic significance of acetylated 2 ''-O-glycosyl-6-c-hexosyl-flavones and GOTcAB saponins with methoxycinnamoyl-substituted alpha-chains was discussed.

关键词： Saponins GOTcAB Antioxidant and enzyme inhibitory activity Gypsophila perfoliata tekirae c O-flavonoid glycosides

来源：评论

学校读者我要写书评

暂无评论

The nuclear ubiquitin ligase adaptor SPOP is a conserved regulator of c9orf72 dipeptide toxicity

引用

PROcEEDINGS OF THE NATIONAL AcADEMY OF ScIENcES OF THE UNITED STATES OF AMERIcA 2021年第40期118卷 e2104664118页

作者： Snoznik, carley Medvedeva, Valentina Mojsilovic-Petrovic, Jelena Rudich, Paige Oosten, James Kalb, Robert G. Lamitina, Todd Univ Pittsburgh Childrens Hosp Pittsburgh Dept Pediat Div Child NeurolMed Ctr Pittsburgh PA 15224 USA Northwestern Univ Feinberg Sch Med Dept Neurol Chicago IL 60611 USA Univ Pittsburgh Grad Program Cell Biol & Mol Physiol Med Ctr Pittsburgh PA 15261 USA Univ Pittsburgh Dept Cell Biol Sch Med Pittsburgh PA 15261 USA

A hexanucleotide repeat expansion in the c9orf72 gene is the most common cause of inherited amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Unconventional translation of the c9orf72 repeat produces dipeptide repeat proteins (DPRs). Previously, we showed that the DPRs PR50 and GR50 are highly toxic when expressed in caenorhabditis elegans , and this toxicity depends on nuclear localization of the DPR. In an unbiased genome-wide RNA interference (RNAi) screen for suppressors of PR50 toxicity, we identified 12 genes that consistently suppressed either the developmental arrest and/or paralysis phenotype evoked by PR50 expression. All of these genes have vertebrate homologs, and 7 of 12 contain predicted nuclear localization signals. One of these genes was spop-1 , the c. elegans homolog of SPOP , a nuclear localized E3 ubiquitin ligase adaptor only found in metazoans. SPOP is also required for GR50 toxicity and functions in a genetic pathway that includes cul-3 , which is the canonical E3 ligase partner for SPOP. Genetic or pharmacological inhibition of SPOP in mammalian primary spinal cord motor neurons suppressed DPR toxicity without affecting DPR expression levels. Finally, we find that knockdown of bromodomain proteins in both c. elegans and mammalian neurons, which are known SPOP ubiquitination targets, suppresses the protective effect of SPOP inhibition. Together, these data suggest a model in which SPOP promotes the DPR-dependent ubiquitination and degradation of BRD proteins. We speculate the pharmacological manipulation of this pathway, which is currently underway for multiple cancer subtypes, could also represent an entry point for therapeutic intervention to treat c9orf72 FTD/ALS.

关键词： c elegans neurodegeneration genetic screen bromodomain proteins proteasome

来源：评论

学校读者我要写书评

暂无评论

Investigating the effect of heat treatment on B 4 c reinforced aluminum metal matrix composites

引用

Materials Today: Proceedings 2021年 45卷 100-104页

作者： Srinivas Reddy Mungara H.S. Manohar Shashank Dubey Sharan Teja Reddy Dept. of Mech. Engg. CMR Institute of Technology Bengaluru 560037 India Department of Mechanical Engineering Dayananda Sagar Academy of Technology and Management Bengaluru 560082 India Department of Mechanical Engineering CMR Institute of Technology Bengaluru 560037 India School of Mechanical Engineering REVA University Bengaluru 560064 India

The present work focuses on investigation of tensile and compressive strengths of AA7050-B 4 c composites before heat treatment (as-cast) and post heat treatment. composites with 2, 4, 6 and 8 wt% of B 4 c were prepared using bottom pouring stir-casting furnace. Microstructural analysis done using EDS and SEM results affirmed that the B 4 c particulates were homogeneously scattered in the alloy matrix. Solution heat treatment was carried out on cast samples at 530 °c for a period of 2 h. After solution, T6 heat treatment (Artificial Ageing) was performed on the samples at 180 °c for a period of 8 h. Mechanical strength properties- yield strength, ultimate tensile strength and compression strength, were determined according to ASTM standards.

关键词： AA7050 B 4 c Stir casting Artificial ageing Tensile strength compression strength

来源：评论

学校读者我要写书评

暂无评论

c/EBP homologous protein deficiency enhances hematopoietic stem cell function via reducing ATF3/ROS-induced cell apoptosis

引用

AGING cELL 2021年第7期20卷 e13382页

作者： Shi, Zhencan Diao, Daojun Zhao, Yanan Luo, Ying Li, Yafei Liu, Dingdong Zhang, Kai Qiu, Yugang Yu, Li Song, Zhangfa Ju, Zhenyu Jinan Univ Inst Aging & Regenerat Med Minist Educ Key Lab Regenerat Med Guangzhou Peoples R China Weifang Med Univ Sch Rehabil Med Weifang Peoples R China Zhejiang Univ Sir Run Run Shaw Hosp Dept Colorectal Surg Hangzhou Peoples R China

Hematopoietic stem cells (HScs) reside in a quiescent niche to reserve their capacity of self-renewal. Upon hematopoietic injuries, HScs enter the cell cycle and encounter protein homeostasis problems caused by accumulation of misfolded proteins. However, the mechanism by which protein homeostasis influences HSc function and maintenance remains poorly understood. Here, we show that c/EBP homologous protein (cHOP), demonstrated previously to induces cell death upon unfolded protein response (UPR), plays an important role in HScs regeneration. cHOP-/- mice showed normal hematopoietic stem and progenitor cell frequencies in steady state. However, when treated with 5-FU, cHOP deficiency resulted in higher survival rates, associated with an increased number of HScs and reduced level of apoptosis. In serial competitive transplantation experiments, cHOP-/- HScs showed a dramatic enhancement of repopulation ability and a reduction of protein aggresomes. Mechanistically, cHOP deletion causes reduced ATF3 expression and further leads to decreased protein aggregation and ROS. In addition, cHOP-/- HScs exhibited an increased resistance to IR-induced DNA damage and improved HScs homeostasis and function in telomere dysfunctional (G3Terc(-/-)) mice. In summary, these findings disclose a new role of cHOP in the regulation of the HScs function and homeostasis through reducing ATF3 and ROS signaling.

关键词： apoptosis ATF3 c EBP homologous protein hematopoietic stem cell function ROS

来源：评论

学校读者我要写书评

暂无评论

A DNA Damage-Repair Dynamic Model for HRS/IRR Effects of c.elegans Induced by Neutron Irradiation

引用

DOSE-RESPONSE 2021年第1期19卷 15593258211001254-15593258211001254页

作者： Feng, Guangyan Zhang, Lianxin Yang, Zhanguo Zhang, Yong Zhang, Siwei Li, Taosheng Xu, Zhao Anhui Univ Inst Phys Sci & Informat Technol Hefei Anhui Peoples R China Chinese Acad Sci Hefei Inst Phys Sci Hefei Anhui Peoples R China Univ Sci & Technol China Hefei Anhui Peoples R China

Neutron irradiation which could trigger severe biological effects, is being applied in nuclear plants, radiotherapy, and aerospace gradually. Low dose hyper-radiosensitivity response of low Linear Energy Transfer (LET) irradiation on the cell survival has become a matter of great interest since its discovery, but a few research have been done on this response induced by neutron irradiation. To investigate this response induced by neutron irradiation, caenorhabditis elegans (c. elegans) was irradiated by neutron irradiation. The surviving fraction of c. elegans on the 12th day after irradiation was analyzed, which showed a hyper-radiosensitive response at low doses and followed by an increase in survival fraction at slightly higher doses. The finding of this work that neutron irradiation decreased the surviving fraction in a non-dose-dependent manner was different from previous low-LET irradiation studies. To understand the experimental results, a DNA damage-repair model was introduced. By comparing experimental results with theoretical analyses, we suggest that the low dose hyper-radiosensitivity response of neutron irradiation may possible related to different radiation types and DNA damage recognition proteins and immune system of c. elegans.

关键词： hyper-radiosensitivity neutron c elegans surviving-fraction damage-repair model

来源：评论

学校读者我要写书评

暂无评论

Molecular analysis of cryptosporidiosis cases in Western Australia in 2019 and 2020 supports the occurrence of two swimming pool associated outbreaks and reveals the emergence of a rare c. hominis IbA12G3 subtype

引用

INFEcTION GENETIcS AND EVOLUTION 2021年 92卷 104859页

作者： Braima, Kamil Zahedi, Alireza Egan, Siobhon Austen, Jill Xiao, Lihua Feng, Yaoyu Witham, Benjamin Pingault, Nevada Perera, Shalinie Oskam, charlotte Reid, Simon Ryan, Una Murdoch Univ Ctr Biosecur & One Hlth Harry Butler Inst Perth WA 6150 Australia Univ Queensland Fac Med Sch Publ Hlth Herston Qld 4006 Australia South China Agr Univ Coll Vet Med Key Lab Zoonosis Minist Agr Guangzhou 510642 Peoples R China OzFoodNet Communicable Dis Control Directorate Perth WA Australia Western Diagnost Pathol Perth WA 6154 Australia

cryptosporidium is an important protozoan parasite and due to its resistance to chlorine is a major cause of swimming pool-associated gastroenteritis outbreaks. The present study combined contact tracing and molecular techniques to analyse cryptosporidiosis cases and outbreaks in Western Australia in 2019 and 2020. In the 2019 outbreak, subtyping at the 60 kDa glycoprotein (gp60) gene identified 89.0% (16/18) of samples were caused by the c. hominis IdA15G1 subtype. Amplicon next generation sequencing (NGS) at the gp60 locus identified five c. hominis IdA15G1 subtype samples that also had c. hominis IdA14 subtype DNA, while multi locus sequence typing (MLST) analysis on a subset (n = 14) of c. hominis samples identified three IdA15G1 samples with a 6 bp insertion at the end of the trinucleotide repeat region of the cp47 gene. In 2020, 88.0% (73/83) of samples typed were caused by the relatively rare c. hominis subtype IbA12G3. Four mixed infections were observed by NGS with three IdA15G1/ IdA14 mixtures and one c. parvum IIaA18G3R1 sample mixed with IIaA16G3R1. No genetic diversity using MLST was detected. Epidemiological and molecular data indicates that the outbreaks in 2019 and 2020 were each potentially from swimming pool point sources and a new c. hominis subtype IbA12G3 is emerging in Australia. The findings of the present study are important for understanding the introduction and transmission of rare cryptosporidium subtypes to vulnerable populations.

关键词： cryptosporidium Outbreak c Swimming pool Next-generation sequencing Multi locus sequence typing

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：