Hepatocellular carcinoma(Hcc) is the fifth most common cancer, and hepatitis c virus(HcV) infection plays a major role in Hcc development. The molecular mechanisms by which HcV infection leads to Hcc are varied. HcV c...
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Hepatocellular carcinoma(Hcc) is the fifth most common cancer, and hepatitis c virus(HcV) infection plays a major role in Hcc development. The molecular mechanisms by which HcV infection leads to Hcc are varied. HcV core protein is an important risk factor in HcV-associated liver pathogenesis and can modulate several signaling pathways involved in cell cycle regulation, cell growth promotion, cell proliferation, apoptosis, oxidative stress and lipid metabolism. The dysregulation of signaling pathways such as transforming growth factor β(TGF-β), vascular endothelial growth factor(VEGF), Wnt/β-catenin(WNT), cyclooxygenase-2(cOX-2) and peroxisome proliferator-activated receptor α(PPARα) by HcV core protein is implicated in the development of Hcc. Therefore, it has been suggested that this protein be considered a favorable target for further studies in the development of Hcc. In addition, considering the axial role of these signaling pathways in Hcc, they are considered druggable targets for cancer therapy. Therefore, using strategies to limit the dysregulation effects of core protein on these signaling pathways seems necessary to prevent HcV-related Hcc.
In the context of protecting Mg-based nano-objects for potential hydrogen storage applications, the potential of c:H layer as a barrier polymer material deposited by the plasma-enhanced chemical vapor deposition proce...
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In the context of protecting Mg-based nano-objects for potential hydrogen storage applications, the potential of c:H layer as a barrier polymer material deposited by the plasma-enhanced chemical vapor deposition process is examined. corrosion tests reveal (a) good barrier properties of the c:H layer and (b) suggest an increase in the internal stress with the power dissipated in the plasma. The latter is attributed to an increase in the cross-linking density of the coatings accompanied by an increase in the stiffness as shown by nanoindentation measurements. Finally, for a given set of plasma parameters, Mg-based nanowires were successfully enrobed by the c:H coatings as evidenced by scanning electron microscopy measurements.
During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this 'chromosomal dance' still remainunclear. We...
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During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this 'chromosomal dance' still remainunclear. We identified DEB-1 - an orthologue of mammalian vinculin - as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINccomplex. In early meiotic nuclei of c. elegans, DEB-1 is localized to the nuclear periphery and alongside the synaptonemal complex of paired homologues. Upon DEB-1 depletion, chromosomes attached to SUN-1 foci remain highly motile until late pachytene. Although the initiation of homologue pairing started normally, irregularities in the formation of the synaptonemal complex occur, and these results in meiotic defects such as increased number of univalents at diakinesis and high embryonic lethality. Our data identify DEB-1 as a new player regulating chromosome dynamics and pairing during meiotic prophase I.
carbon material doped with nitrogen and transition metal is a kind of promising candidate of the platinum for oxygen reduction reaction (ORR) process due to its low cost, efficiency and stability. Here we demonstrate ...
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carbon material doped with nitrogen and transition metal is a kind of promising candidate of the platinum for oxygen reduction reaction (ORR) process due to its low cost, efficiency and stability. Here we demonstrate an original type of Fe/N/ccatalyst based on pore-in-pore structures (P-P Fe/N/c), showing one of the highest oxygen reduction reaction performances among all reported Fe/N/c-type catalysts (onset potential of 0.995 V, half-wave potential of 0.881 V vs. RHE with a relatively low mass loading of 0.32 mg cm(-2) and long-term durability (97% relative current in 60 000 s operation) in alkaline media. Such outstanding performances can be ascribed to the efficient active sites activated by the encapsulated atomic and subnanoscale iron, and great exposure of these active sites due to the unique pore-in-pore hierarchical construction. Once assembled in lithium-O-2 batteries, a specificcapacity of 7250 mA h g(-1) at 70 mA g(-1) can be obtained by the P-P Fe/N/ccatalyst. Moreover, upon cycling, the P-P Fe/N/c electrode can be cycled 150 times with no capacity loss, which is much longer than six cycles of pure Super P air electrode. These results evidently reveal the developed Fe/N/ccatalyst holds great promise to serve as an alternative to the conventional Pt-based noble metal catalysts.
Dendriticcells (Dcs) are key players in immunity and tolerance. Some Dcs express c-kit, the receptor for stem cell factor (ScF), nevertheless c-kit functional role and the regulation of its expression in Dcs are inco...
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Dendriticcells (Dcs) are key players in immunity and tolerance. Some Dcs express c-kit, the receptor for stem cell factor (ScF), nevertheless c-kit functional role and the regulation of its expression in Dcs are incompletely defined. We recently demonstrated that autocrine ScF sustains a pro-survival circuit, and that ScF increases phospho-AKT in c-kit+ mouse bone marrow-derived Dcs (BMdDcs). Herein we observed that cpG and PolyI:c, two stimuli mimicking bacterial and viral nucleic acids respectively, strongly inhibited c-kit expression by BMdDcs and spleen Dcs in vitro and in vivo. Experiments in IFNARI(-/-) mice showed that IFN-I pathway was required for c-kit down-regulation in cDc1s, but only partially supported it in cDc2s. Furthermore, cpG and PolyI:c strongly inhibited c-kit mRNA expression. In agreement with the reduced c-kit levels, ScF pro-survival activity was impaired. Thus in the presence of exogenously provided ScF, either PolyI:c or cpG induced spleen Dc death in 2 days, while at earlier times IL-6 and IL-12 production were slightly increased. In contrast, ScF improved survival of unstimulated spleen Dcs expressing high c-kit levels. Our studies suggest that c-kit down-modulation is a previously neglected component of Dc response to cpG and PolyI:c, regulating Dc survival and ultimately tuning immune response.
Shwachman-Diamond syndrome is a rare autosomal recessive disorder (SDS;OMIM #260400) mainly characterized by bone marrow failure, exocrine pancreatic insufficiency, and skeletal defects. The first causative gene is SB...
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Shwachman-Diamond syndrome is a rare autosomal recessive disorder (SDS;OMIM #260400) mainly characterized by bone marrow failure, exocrine pancreatic insufficiency, and skeletal defects. The first causative gene is SBDS, identified in 2003, and patients clinically affected by SDS carry mainly SBDS mutations, and most of them are represented by common pathogenic variants derived from geneticconversion events between SBDS and its pseudogene, SBDSP1. The Italian Registry of SDS collected the index cases who received a clinical SDS diagnosis with the demonstration of SBDS biallelic mutations;one of these is always represented by the [c.258+2T>c], while the second one includes either the [c.183_184delinsTA>cT] or [c.183_184delinsTA>cT+c.258+2T>c] or [c.258+2T>c] in, respectively, 59, 12.5 and 10.83% of cases entered until May 2022. In the 17,67% the SBDS genotype is completed by fourteen different types of rare, private, pathogenic variants and all these alleles were observed once among Italian cases, except for [c.187G>T] and [c.258+533_459+403del] alleles, which were observed in two and four unrelated families, respectively. The first one is present in two families, both originating from Northern Tuscany, while for the second allele, the deletion, three families originate from Sicily and one from Lazio. The presence of the deletion in four families (corresponding to 3.31% of index cases), pushed us to investigate if it could be due to a founder effect, with the same mutation originating from a single ancestral event in all cases. Genotyping the region surrounding the SBDS locus, we defined a common haplotype in the families coming from Sicily, while a completely different one resulted in the family originated from Lazio. This result, identifying the most likely ancestral haplotype associated with the deletion of exon 3, suggested a potential founder effect that might be localized in the provinces of Palermo and Messina, in the middle of the North of Sicily. Our result
In this paper, a significant enhancement in current efficiency of the green tandem organic light-emitting diodes(TOLEDs) is demonstrated, which is based on a buffer-modified charge generation layer(cGL) of fullerene c...
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In this paper, a significant enhancement in current efficiency of the green tandem organic light-emitting diodes(TOLEDs) is demonstrated, which is based on a buffer-modified charge generation layer(cGL) of fullerene carbon(c60)/zinc-phthalocyanine(ZnPc). Al and MoO3 were used as the buffer-modified layers on both sides of the bilayer c60/ZnPc, respectively. Experimental results show that the inserted Al and MoO3 layers can effectively increase the electron extraction of the cGL for obtaining the device performance enhancement. compared with that of the green TOLEDs without buffer-modified layers in cGL(37.3 cd·A-1), the current efficiency of the green TOLEDs is increased to 54.1 cd·A-1. Further study results find that the performance can also be improved by optimizing the thickness of Al in the cGL. The maximum current efficiency and maximum luminance of the green TOLEDs achieve 63.5 cd·A-1 and 17 873 cd·m-2, respectively, when the multilayer structure of the cGL is Al(3 nm)/c60(5 nm)/ZnPc(5 nm)/MoO3(3 nm).
A facile synthetic approach to access of 3H- pyrrolo[ 2,3c] quinoline derivatives has been achieved by a sequential I2- promoted cyclization/ Staudinger/ aza- Wittig/ dehydroaromatization reaction. The targeted produc...
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A facile synthetic approach to access of 3H- pyrrolo[ 2,3c] quinoline derivatives has been achieved by a sequential I2- promoted cyclization/ Staudinger/ aza- Wittig/ dehydroaromatization reaction. The targeted products were received in moderate to good yields ( 62- 81%). The broad substrate scope and easy availability of the starting materials make this method a valuable tool for generating 3H- pyrrolo[ 2,3- c] quinoline products.
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