programs written in Applesoft BASIC for the rapid acquisition and evaluation of data from a commercially available microtiter plate fluorescence reader are presented. Using the data acquisition program, the relative f...
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programs written in Applesoft BASIC for the rapid acquisition and evaluation of data from a commercially available microtiter plate fluorescence reader are presented. Using the data acquisition program, the relative fluorescence readings from all 96 wells of the microtiter plate (one read cycle of the fluorescence reader) can be stored in each of up to 90 consecutively numbered files on a single-sided diskette. A simple timer circuit is described which, when used in conjunction with the above program, initiates the fluorescence reading process at preset time intervals, thus making automatic acquisition of data possible. A further program plots the data from consecutive files on the computer monitor and prints a hard copy if required. The feasibility of applying the above system and software to kinetic measurements in enzyme systems is demonstrated using methylumbelliferyl phosphate and an alkaline phosphatase/immunoglobulin conjugate. In addition, its use in following the formation of extracellular hydrogen peroxide by stimulated polymorphonuclear leukocytes using horseradish peroxidase-coupled oxidation of the fluorescent compound 7-hydroxy-6-methoxy-coumarin (scopoletin) is described.
computer programs are presented for automating the collection, processing, and analysis of enzyme-linked immunosorbent assay (ELISA) data. The programs are written to run on an Apple Macintosh computer (equipped with ...
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computer programs are presented for automating the collection, processing, and analysis of enzyme-linked immunosorbent assay (ELISA) data. The programs are written to run on an Apple Macintosh computer (equipped with at least 512 kilobytes of random access memory, a single disk drive, and an Apple Image-writer dot matrix printer) interfaced with a Bio-Rad EIA reader. The primary program is written in Pascal with associated data analysis performed by a programmable electronic spreadsheet.
Based on a comparative analysis of various modifications of the “shooting” method, used for solving the radial Schrödinger equation, a new algorithm is proposed, which allows to increase considerably its soluti...
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Based on a comparative analysis of various modifications of the “shooting” method, used for solving the radial Schrödinger equation, a new algorithm is proposed, which allows to increase considerably its solution accuracy. This is achieved, first, by rising the order of approximation of the Schrödinger equation fromh4 (in the traditional Numerov-Cooley method) up toh6 and, second, by using the variable integration steph. The computer program has been written in Fortran IV to execute this algorithm for calculating vibrational wave functions and related quantities for diatomic molecules.
The increasingly sophisticated techniques for the extraction of information from ultrasonic data entail a corresponding increase in complexity of the software used. An interactive signal and image processing package, ...
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The increasingly sophisticated techniques for the extraction of information from ultrasonic data entail a corresponding increase in complexity of the software used. An interactive signal and image processing package, called TWODIMSY, has been developed at the Ultrasonics Institute to allow non-programmers an entry to digital signal processing, and to simplify the task of staff involved in programming. We present an outline of the design considerations which shaped TWODIMSY, and a brief history of its ancestors. A detailed description of the package is given, including the type of computational facilities provided and the approach taken to solve the problems of data storage, operator interface and documentation.
Abstract The successful introduction of microcomputers into the curriculum is crucially dependent on software quality. Evaluations of programs must be based on educational rather than technological criteria. The avai...
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A flexible APL computer program is presented for assistance in planning clinical trials designed to test the equivalence of two therapies. It is a substitute for the sample size graphs of Blackwelder and Chang and pro...
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A flexible APL computer program is presented for assistance in planning clinical trials designed to test the equivalence of two therapies. It is a substitute for the sample size graphs of Blackwelder and Chang and provides the option to calculate any of the six characteristics-sample size, significance level, power, success probability of standard therapy, success probability of experimental therapy, and minimum difference of interest-if five of them are prespecified.
Comparisons are made between BILOG version 2.2 and LOGIST 5.0 Version 2.5 in estimating the item parameters, traits, item characteristic functions (ICFs), and test characteristic functions (TCFs) for the three-paramet...
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Comparisons are made between BILOG version 2.2 and LOGIST 5.0 Version 2.5 in estimating the item parameters, traits, item characteristic functions (ICFs), and test characteristic functions (TCFs) for the three-parameter logistic model. Data analyzed are simulated item responses for 1000 simulees and one 10-item test, four 20-item tests, and four 40-item tests. LOGIST usually was faster than BILOG in producing maximum likelihood estimates. BILOG almost always produced more accurate estimates of individual item parameters. In estimating ICFs and TCFs BILOG was more accurate for the 10-item test, and the two programs were about equally accurate for the 20- and 40-item tests.
One of the problems of derivative spectrophotometry, the decrease of signal-to-noise ratio by derivative operations, was solved by three concepts of digital filtering, ensemble averaging, least squares polynomial smoo...
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