检索结果-内蒙古大学图书馆

Selection of hyperfunctional siRNAs with improved potency and specificity

NUCLEIC ACIDS RESEARCH 2009年第22期37卷 e152-e152页

作者： Wang, Xiaowei Wang, Xiaohui Varma, Rajeev K. Beauchamp, Lesslie Magdaleno, Susan Sendera, Timothy J. Washington Univ Sch Med Dept Radiat Oncol St Louis MO 63108 USA Appl Biosyst Inc Austin TX 78744 USA

One critical step in RNA interference (RNAi) experiments is to design small interfering RNAs (siRNAs) that can greatly reduce the expression of the target transcripts, but not of other unintended targets. Although various statistical and computational approaches have been attempted, this remains a challenge facing RNAi researchers. Here, we present a new experimentally validated method for siRNA design. By analyzing public siRNA data and focusing on hyperfunctional siRNAs, we identified a set of sequence features as potency selection criteria to build an siRNA design algorithm with support vector machines. Additional bioinformatics filters were also included in the algorithm to increase RNAi specificity by reducing potential sequence cross-hybridization or microRNA-like effects. Independent validation experiments were performed, which indicated that the newly designed siRNAs have significantly improved performance, and worked effectively even at low concentrations. Furthermore, our cell-based studies demonstrated that the siRNA off-target effects were significantly reduced when the siRNAs were delivered into cells at the 3 nM concentration compared to 30 nM. Thus, the capability of our new design program to select highly potent siRNAs also renders increased RNAi specificity because these siRNAs can be used at a much lower concentration. The siRNA design web server is available at http://***/tools/sidesign/.

关键词： .RNA interference analyzing Life Technologies design algorithm designed siRNAs

来源：评论

学校读者我要写书评

暂无评论

A delimitation of the support of optimal designs for Kiefer's φ_p-class of criteria

引用

STATISTICS & PROBABILITY LETTERS 2013年第12期83卷 2721-2728页

作者： Pronzato, Luc Univ Nice Sophia Antipolis CNRS Lab I3S F-06903 Sophia Antipolis France

The paper extends the result of Harman and Pronzato [Harman, R., Pronzato, L., 2007. Improvements on removing non-optimal support points in D-optimum design algorithms. Statistics & Probability Letters 77, 90-94], which corresponds to p = 0, to all strictly concave criteria in Kiefer's phi(p)-class. We show that, for any given design measure any support point x(*) of a phi(p)-optimal design is such that the directional derivative of phi(p) at xi in the direction of the delta measure at x(*) is larger than some bound h(p) [xi] which is easily computed. (C) 2013 Elsevier B.V. All rights reserved.

关键词： Approximate design Optimum design Support points design algorithm

来源：评论

学校读者我要写书评

暂无评论

Continuous selective heating of the solid phase of a dispersion

引用

THEORETICAL FOUNDATIONS OF CHEMICAL ENGINEERING 2003年第1期37卷 25-31页

作者： Aliev, MR Aliev, RZ Aliev, AR Dagestan Res Inst Viticulture & Proc Prod Derbent Raion 368621 Dageston Russia Russian Acad Sci Amirkhanov Inst Phys Dagestan Sci Ctr Makhachkala 367003 Dagestan Russia

A new technique is suggested for the continuous and selective heating of the solid phase in a flowing dispersion. This technique is implemented in a heater-valve-pulsed apparatus unit. Mathematical models are developed for the cocurrent and countercurrent designs of the heater. Analytical solutions are obtained for the model equations, and parameters governing the continuous selective heating of the solid phase are determined. A design algorithm is suggested for the unit.

关键词： design algorithm new technique COCURRENT Solid phases edge heating

来源：评论

学校读者我要写书评

暂无评论

AN algorithm FOR SYNTHESIS OF MULTIPLE-OUTPUT COMBINATIONAL LOGIC

引用

IEEE TRANSACTIONS ON COMPUTERS 1968年第2期C 17卷 117-+页

作者： SCHNEIDER, PR DIETMEYER, DL IEEE

Abstract—A computer-oriented algorithm for synthesizing combinational logic circuits from a collection of functionally packaged circuits is developed. The algorithm uses a hierarchy of "goals" in an iterative decision process in a manner similar to that employed by theorem proving and gamne playing programs. With each iteration a set of "tasks" finds the circuit package which satisfies the highest level goal while meeting circuit constraints.

关键词： Index terms—Circuit constraints combinational logic synthesis decomposition design algorithm design goals logic design automation module library.

来源：评论

学校读者我要写书评

暂无评论

A static output feedback control for linear systems

引用

AUTOMATION AND REMOTE CONTROL 2004年第4期65卷 559-566页

作者： Pakshin, PV Ryabov, AV Nizhni Novgorod State Tech Univ Arzamas Affiliated Inst Arzamas Russia

Necessary and sufficient condition for the stabilizability of linear discrete systems by a static output feedback control are formulated and used in designing algorithms for robust stabilizing controls and controls that ensure concurrent stabilization of a family of discrete systems. algorithms are implemented by MATLAB and SCILAB with LMISOLVER and SeDuMi interfaces. The results are generalized to random-structure systems.

关键词： MATLAB stabilizability Discrete systems Output Feedback Control linear discrete system design algorithm static output Necessary feedback control Linear system

来源：评论

学校读者我要写书评

暂无评论

Exploring the origins of binding specificity through the computational redesign of calmodulin

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2003年第23期100卷 13274-13279页

作者： Shifman, JM Mayo, SL CALTECH Howard Hughes Med Inst Pasadena CA 91125 USA CALTECH Div Biol Pasadena CA 91125 USA

Calmodulin (CaM) is a second messenger protein that has evolved to bind tightly to a variety of targets and, as such, exhibits low binding specificity. We redesigned CaM by using a computational protein design algorithm to improve its binding specificity for one of its targets, smooth muscle myosin light chain kinase (smMLCK). Residues in or near the CaM/smMLCK binding interface were optimized;CaM interactions with alternative targets were not directly considered in the optimization. The predicted CaM sequences were constructed and tested for binding to a set of eight targets including smMLCK. The best CaM variant, obtained from a calculation that emphasized intermolecular interactions, showed up to a 155-fold increase in binding specificity. The increase in binding specificity was not due to improved binding to smMLCK, but due to decreased binding to the alternative targets. This finding is consistent with the fact that the sequence of wild-type CaM is nearly optimal for interactions with numerous targets.

关键词： design algorithm origins Calmodulin Specificity

来源：评论

学校读者我要写书评

暂无评论

A method and algorithms for veering a flying apparatus from the spatial constraint surface

引用

AUTOMATION AND REMOTE CONTROL 2001年第7期62卷 1042-1048页

作者： Akimov, AN Vorob'ev, VV Mil Aviat Tech Univ Moscow Russia

An approach to designing algorithms for confining constraints within the aircraft control trajectory is elaborated on the basis of geometric transformations in three-dimensional state space. algorithms are designed to confine the constraints for airborne control complexes and adaptive to the current coordinate-parametric state of the object and configuration of the constraining surface. Formulas for computing the aircraft position angles in space during maneuvers to veer off from the constraining surface are derived. The veer-off trajectory is realized by direct optimization tools and algorithms. An example is given to illustrate to veer off from surface of a paraboloid of rotation.

关键词： Control trajectory Surfaces Constraint Aircraft control design algorithm Geometric transformation algorithms paraboloidal trajectory Flying Self tuning

来源：评论

学校读者我要写书评

暂无评论

Digital designs of broadband visible antireflection coating for wide angular incidence

引用

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS 2005年第1A期44卷 163-167页

作者： Liou, YY Liu, YT Chienkuo Technol Univ Dept Elect Engn Changhua 500 Taiwan Nan Jeon Inst Technol Gen Educ Ctr Tainan 737 Taiwan

A design algorithm based on a modified minimizing search technique is applied to design a visible antireflection coating for a wide angular incidence with an angle up to 30degrees from the normal. The design approach consisting of tuning and minimizing operations is, effective in refining the antireflective performance and simplifying the design structure of the desired solution. It is shown that the maximum wide angular visible spectral reflectance of minimizing search antireflection coating designs for a three-material 60-layer system is reduced to less than 0.490% with 15-21-layered structures, and the average visible spectral reflectance for each incident angle is reduced to below 0.137%.

关键词： minimizing search multilayer antireflection coating design algorithm visible spectral region wideband

来源：评论

学校读者我要写书评

暂无评论

A goal-oriented planning approach for two-dimensional cutting components in architectural design: Coupling BIM and Parametric design (PD)

引用

JOURNAL OF BUILDING ENGINEERING 2023年 76卷

作者： Wu, Shihai Lu, Wei-Zhen Qin, Chao Wen, Baohua Wu, Dizi Xiang, Yujing Changsha Univ Sci & Technol Sch Architecture Changsha 410004 Peoples R China City Univ Hong Kong Dept Architecture & Civil Engn Hong Kong Peoples R China Hunan Univ Sch Architecture & Planning Changsha 410082 Peoples R China Cent South Univ Sch Resources & Safety Engn Changsha 410083 Peoples R China

Two-dimensional (2-d) cutting components are essential in the Architecture, Engineering, and Construction (AEC) industry. However, due to the complexity of computing cut components' reuse, the 2-d cutting component still lacks appropriate design-aid tools to generate accurate graphical and numerical results in the architectural design process, leading to material waste and hindering the entry of intelligent technologies. This research proposed a grid-based planning workflow based on the BIM and parametric design (PD) to accurately generate and optimize 2d cutting components' planning. The proposed workflow combined the "2-d bin packing problem" problem-solving method in operations research to compute the components' reuse. Moreover, an evolutionary algorithm was used to enable the workflow can goal-oriented optimize the design alternatives according to requirements, expanding the workflow's adaptability. A prototype system was developed to verify the proposed workflow. The results show that compared with current design-aid approaches, the proposed workflow can generate accurate graphical and numerical results while reducing material waste by 7%-12% due to the refined design. The outcomes improve the intelligence of the AEC industry while promoting the industry's sustainable development.

关键词： Two-dimensional cutting components Parametric design Goal-oriented optimization design algorithm Applied mathematics

来源：评论

学校读者我要写书评

暂无评论

A QUADRATICALLY CONVERGENT algorithm FOR SOLVING INFINITE DIMENSIONAL INEQUALITIES

引用

APPLIED MATHEMATICS AND OPTIMIZATION 1982年第1期9卷 25-40页

作者： MAYNE, DQ POLAK, E UNIV CALIF BERKELEY DEPT ELECT ENGN & COMP SCIBERKELEYCA 94720 UNIV CALIF BERKELEY ELECTR RES LABBERKELEYCA 94720

Many design algorithms can be formulated as determining a set of parameters to satisfy conventional and infinite dimensional constraints. An algorithm, with quadratic rate of convergence, for solving such inequalities... 详细信息

关键词： System Theory Mathematical Method design algorithm Convergent algorithm Quadratic Rate

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：