The recent development of small, high-efficiency columns for rapid HPLC was investigated for application to the determination of steroids. Two applications are described, the determination of estrogenic steroids in ur...
详细信息
The recent development of small, high-efficiency columns for rapid HPLC was investigated for application to the determination of steroids. Two applications are described, the determination of estrogenic steroids in urine and the determination of progesterone and estradiol benzoate in an injection formulation. Methods were developed for both these applications, each with over-all run times of less than 6 min, representing a considerable reduction over previously used methods. These methods were extensively validated and were found to be accurate, robust and reliable. The problem of reduced sample loadability is considered and the practical solution of peak compression illustrated.
The metabolites of the oral anticoagulant phenprocoumon are characterized as the 7,4′ and 6-hydroxyphenprocoumons in human plasma and urine using liquid chromatography and gas chromatography-mass spectrometry. The au...
详细信息
The metabolites of the oral anticoagulant phenprocoumon are characterized as the 7,4′ and 6-hydroxyphenprocoumons in human plasma and urine using liquid chromatography and gas chromatography-mass spectrometry. The author's own work and that of others are reviewed and the metabolic stereoselectivity of phenprocoumon and its metabolism is compared with that of warfarin.
暂无评论