检索结果-内蒙古大学图书馆

A "DropChip" cell array for DNA and siRNA transfection combined with drug screening

Nanobiotechnology 2005年第2期1卷 183-189页

作者： Schaack, Béatrice Reboud, Julien Combe, Stéphanie Fouqué, Brigitte Berger, François Boccard, Sandra Filhol-Cochet, Odile Chatelain, François Laboratoire Biopuces Bat. 4020 CEA F-38054 Grenoble Cedex 9 17 rue des martyrs France INSERM EMI 104 Bat. 4020 CEA F-38054 Grenoble Cedex 9 17 rue des martyrs France INSERM U318 University Hospital of Grenoble F-38043 Grenoble France

We have developed a "DropChip" microarray for multiplexed cell-based assays to perform information-rich high-throughput screening. This microarray features cell culture nanoliter droplets on a glass slide;the culture's size, shape, and location are accurately controlled by surface tension. We report parallel gene expression and gene silencing analyses on the same array, combining nucleic acid transfection, anti-cancer drug screening, and cell cultures. In particular, we analyzed the synergic effects of siRNA and cisplatin on cancerous cells. With up to 100 cells per drop, we could carry out the detection and analysis of cell behavior at the individual cell level, using high-resolution fluorescence microscopy and automated image analysis. Nucleic and chemical compounds require early multiplexed testing using nanomolar quantities. The NanoDrop technology is a unique solution to perform cell-based assay in nanoliter drops, providing a major decrease of cost and time, as well as a precise description of the behavior of several cell types in a single test at the single cell level. This novel cell array format could enable highly informative functional genomic studies and large scale in vitro toxicity testing. Copyright © 2005 Humana Press Inc. All rights of any nature whatsoever are reserved.

关键词： Cell culture nanotechnology expression array High content analysis Multitransfection RNAi

来源：评论

学校读者我要写书评

暂无评论

FIC1, a P-type ATPase linked to cholestatic liver disease, has homologues (ATP8B2 and ATP8B3) expressed throughout the body

引用

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 2003年第2期1633卷 127-131页

作者： Harris, MJ Arias, IM Tufts Univ Sch Med Dept Physiol Boston MA 02111 USA

ATP8B1/FIC1 is a member of the Type IV P-type ATPase family, which function as ATP dependent aminophospholipid translocases (APLT). We identified two familial intrahepatic cholestasis type I (FIC1) homologues, ATP8B2 and ATP8B3, with 53% and 45% amino acid identity, respectively. The expression profile for each gene was determined using a 73-tissue human RNA expression array. The subfamily of FIC1-like proteins is expressed in a wide range of tissues. Given that mutations in FIC1 result in liver disease, these proteins may have important roles in other organs in which they are candidates for genetic and acquired diseases. (C) 2003 Published by Elsevier B.V.

关键词： aminophospholipid translocase PFICI BRIC phospholipid expression array

来源：评论

学校读者我要写书评

暂无评论

Identification of age-dependent changes in expression of senescence-accelerated mouse (SAMP8) hippocampal proteins by expression array analysis

引用

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2000年第3期272卷 657-661页

作者： Kumar, VB Franko, MP Farr, SA Armbrecht, HJ Morley, JE Vet Adm Med Ctr Ctr Geriatr Res Educ & Clin St Louis MO 63125 USA St Louis Univ Hlth Sci Ctr Div Geriatr St Louis MO 63104 USA

Aging is associated with extensive cognitive impairments, although the biochemical and physiological basis of these deficits are unknown. As the hippocampus plays a vital role in cognitive functions, we have selected this tissue to analyze changes in gene expression at two different ages. array technology is utilized to explore how gene expression in hippocampus is affected by accelerated cognitive impairment in Senescence-Accelerated Mouse (SAM P8) strain. We show that the expression of genes associated with stress response and xenobiotic metabolism are strongly affected at a time when cognitive impairment occurs. Affected genes include those involved both in signaling and chaperone function. The effector and regulator family of chaperones, which play an important role in protein folding, and also the xenobiotic metabolizing enzymes that play crucial role in antioxidant systems, show significant changes in gene expression between 4 and 12 months. (C) 2000 Academic Press.

关键词： expression array microarray hybridization protein folding aging mice

来源：评论

学校读者我要写书评

暂无评论

New clustering method for expression array data

引用

Genome Biology 2000年第6期1卷 1-4页

作者： Rachel Brem

An algorithm has been described that clusters expression data from microarray experiments with respect to subsets of genes and other variables, rather than to all data at once.

关键词： array Data expression array Bone Marrow Mononuclear Cell Global Cluster Methodological Innovation

来源：评论

学校读者我要写书评

暂无评论

expression of angiogenic and neurotrophic factors in the human amnion and choriodecidua

引用

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY 2002年第3期187卷 728-734页

作者： Marvin, KW Keelan, JA Eykholt, RL Sato, TA Mitchell, MD Univ Auckland Fac Med & Hlth Sci Liggins Inst Auckland 1 New Zealand Univ Auckland Fac Med & Hlth Sci Div Pharmacol & Clin Pharmacol Auckland 1 New Zealand

OBJECTIVE: Our objective was to identify the novel or differential expression of growth or development associated genes in the human gestational membranes that might play roles in pregnancy or in term or preterm parturition. STUDY DESIGN: Complementary DNA arrays were probed with [alpha(33)P]dCTP-labeled-complementary DNA that was prepared from the RNA of reflected amnion and choriodecidua that represent term not-in-labor, term spontaneous labor, and preterm labor with and without chorioamnionitis (n = 4 per group). Differential expression (term not-in-labor vs term spontaneous labor or preterm labor with chorioamnionitis vs preterm labor without chorioamnionitis) was evaluated by Wilcoxon tests. RESULTS: All 16 amnion samples expressed angiogenic factors (endothelin-2 and -3, vascular endothelial growth factor, and vascular endothelial growth factor-B) and neurotrophic factors (ephrin-A2, ephrin receptors-A2, -B1, -B3, -B4, and -B5, neuropilin-2, p75/nerve growth factor receptor and semaphorin-F). In both amnion and choriodecidua, the expression of vascular endothelial growth factor and the angiopoietin receptor, Tie-2, were greater with term spontaneous labor than with term not-in-labor (P <.05);increased VEGF receptor-2 (flk-1) expression was observed in term spontaneous labor choriodecidua (P <.05) but not amnion. Ephrin-A1 expression increased with term spontaneous labor in both tissues (P <.05). Semaphorin-F expression decreased with preterm labor with chorioamnionitis in choriodecidua (P <.05), although the trend was not significant in amnion (P =. 1). CONCLUSION: Neurotrophic and angiogenic factor genes are expressed in amnion and choriodecidual membranes. Several of the genes exhibit differential expression with labor at term or in association with infection preterm, which suggests roles in or associated with these processes.

关键词： amnion choriodecidua cytokine expression array gestational tissue

来源：评论

学校读者我要写书评

暂无评论

Urea stress is more akin to EGF exposure than to hypertonic stress in renal medullary cells

引用

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY 2002年第3期283卷 F388-F398页

作者： Tian, W Cohen, DM Oregon Hlth Sci Univ Dept Med Div Nephrol Portland OR 97201 USA Oregon Hlth Sci Univ Dept Cell & Dev Biol Portland OR 97201 USA Portland Vet Affairs Med Ctr Portland OR 97201 USA

Although urea is considered to be a cell stressor even in renal medullary cells perpetually exposed to this solute in vivo by virtue of the renal concentrating mechanism, aspects of urea signaling resemble that of a peptide mitogen. Urea was compared with epidermal growth factor and hypertonic NaCl or hypertonic mannitol using a large-scale expression array-based approach. The expression profile in response to urea stress more closely resembled that of EGF treatment than hypertonic stress, as determined by hierarchical cluster analysis;the effect of urea+NaCl was equidistant from that of either solute applied individually. Among the most highly urea- and hypertonicity-responsive transcripts were genes that had previously been shown to be responsive to these solutes, validating this approach. Increased expression of the activating transcription factor 3 by urea was newly detected via expression array and confirmed via immunoblot analysis. Earlier, we noted an abrogation of tonicity-dependent gene regulation by urea, primarily in a transient transfection-based model (Tian W and Cohen DM. Am J Physiol Renal Physiol 280: F904-F912, 2001). Here we applied K-means cluster analysis to demonstrate that the genes most profoundly up- or downregulated by hypertonic stress were partially restored toward basal levels in the presence of urea pretreatment. These global expression data are consistent with our earlier biochemical studies suggesting that urea affords cytoprotection in this context. In the aggregate, these data strongly support the hypothesis that the urea effect in renal medullary cells resembles that of a peptide mitogen in terms of the adaptive program of gene expression and in terms of cytoprotection from hypertonicity.

关键词： expression array tonicity enhancer binding protein hypertonicity cytoprotection activating transcription factor 3 mIMCD3 cells epidermal growth factor

来源：评论

学校读者我要写书评

暂无评论

Protein arrays and microarrays

引用

CURRENT OPINION IN CHEMICAL BIOLOGY 2001年第1期5卷 40-45页

作者： Zhu, H Snyder, M Yale Univ Dept Mol Cellular & Dev Biol New Haven CT 06520 USA Yale Univ Dept Mol Biophys & Biochem New Haven CT 06520 USA

In the past, studies of protein activities have focused on studying a single protein at a time, which is often time-consuming and expensive. Recently, with the sequencing of entire genomes, large-scale proteome analysis has begun. arrays of proteins have been used for the determination of subcellular localization, analysis of protein-protein interactions and biochemical analysis of protein function. New protein-microarray technologies have been introduced that enable the high-throughput analysis of protein activities. These have the potential to revolutionize the analysis of entire proteomes.

关键词： Protein chip protein microarray proteome functional genomics biochemical genomics large-scale expression array protein immobilization affinity tag fusion protein

来源：评论

学校读者我要写书评

暂无评论

Whole-genome expression analysis: challenges beyond clustering

引用

CURRENT OPINION IN STRUCTURAL BIOLOGY 2001年第3期11卷 340-347页

作者： Altman, RB Raychaudhuri, S Stanford Med Informat Stanford CA 95305 USA

Measuring the expression of most or all of the genes in a biological system raises major analytic challenges. A wealth of recent reports uses microarray expression data to examine diverse biological phenomena - from basic processes in model organisms to complex aspects of human disease. After an initial flurry of methods for clustering the data on the basis of similarity, the field has recognized some longer-term challenges. Firstly, there are efforts to understand the sources of noise and variation in microarray experiments in order to increase the biological signal. Secondly, there are efforts to combine expression data with other sources of information to improve the range and quality of conclusions that can be drawn. Finally, techniques are now emerging to reconstruct networks of genetic interactions in order to create integrated and systematic models of biological systems.

关键词： Microarray expression array genomics bioinformatics genetic networks clustering classification data integration Bioinformatics Structural biology

来源：评论

学校读者我要写书评

暂无评论

Signaling and gene regulation by urea in cells of the mammalian kidney medulla

引用

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 2001年第3期130卷 429-436页

作者： Tian, W Cohen, DM Oregon Hlth Sci Univ Div Nephrol Portland OR 97201 USA Oregon Hlth Sci Univ Div Mol Med Portland OR 97201 USA Oregon Hlth Sci Univ Dept Cell & Dev Biol Portland OR 97201 USA Portland Vet Affairs Med Ctr Portland OR 97201 USA

Signaling by urea, although incompletely understood, is relevant both to cells of the mammalian kidney inner medulla and to all cells of the organism in the setting of advanced renal failure with its attendant accumulation of urea in the systemic circulation. The molecular events initiated by urea stress are distinct from those occurring in response to hypertonic stress;urea. activates a characteristic subset of signaling events, which are in large part specific to cultured renal tubular epithelial cells. Interestingly, urea is protective of hypertonic NaCl-inducible apoptosis in this model. Details of this phenomenon are reviewed. The effect of urea has been likened to that of either hypertonicity or of a peptide mitogen. In preliminary expression array analyses, the profile of genes activated by urea stress in renal medullary cells, however, was found to be unique. (C) 2001 Elsevier Science Inc. All rights reserved.

关键词： hypertonicity expression array signal transduction NaCl urea kidney heat shock

来源：评论

学校读者我要写书评

暂无评论

Finding transcription factor binding sequences

引用

Genome Biology 2001年第4期2卷 1-4页

作者： Brem, Rachel

关键词： array Data expression array mRNA Data Yeast Cell Cycle Methodological Innovation

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：