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SUBUNIT COMPOSITION AND COLD STABILITY Of THE PEA COTYLEDON MITOCHONDRIAL f1-ATPASE

BIOCHIMICA ET BIOPHYSICA ACTA 1987年第2期893卷 190-196页

作者： HORAK, A HORAK, H PACKER, M Department of Plant Science The University of Alberta Edmonton Alberta Canada

The f1-ATPase isolated from pea cotyledon sumitochondrial particles contained six types of subunit with molecular weights of 57000 (.alpha.), 55000 (.beta.), 36500 (.gamma.), 26500 (.delta.), 22500 (.delta.'') and 8000 (.epsilon.). The same polypeptide composition was observed even when the purification was carried out at ***.C in the presence of proteolytic inhibitors, suggesting that the sixth subunit was not a proteolytic product formed during the isolation procedure. The six-subunit f1-ATPase exhibited considerable cold stability: it retained 65% of its activity after 24 h of incubation at ***.C and was more than 90% active after 48 h of incubation at ***.C. The 26.5 kDa protein could be dissociated from the remaining f1-ATPase by centrifugation in a linear sucrose gradient containing (NH4)2SO4 and deoxycholate. The resulting five-subunit f1-ATPase was considerably less stable at ***.C than the six-subunit enzyme. Several features suggest the possibility of a functional and structural relationship between the 26.5 kDa a protein of the pea cotyledon mitochondria and the mammalian oligomycin-sensitivity-conferring protein.

关键词： Mitochondrion Mitochondrial f 1 -ATPase Coupling factor f 1 -subunit Cold stability (Pea) f 1 mitochondrial coupling factor PMSf phenylmethylsulfonylfluoride Tricine N -[2-hydroxy-11-bis(hydroxymethyl)ethyl]glycine P i inorganic phosphate

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New compounds from the aerial parts of Justicia gendarussa Burm.f. and their antioxidant and anti-inflammatory activities

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NATURAL PRODUCT RESEARCH 2021年第20期35卷 3478-3486页

作者： Zhang, Hai-Xin Xia, Zhao Xu, Tian-Qi Chen, Yi-Min Zhou, Guang-Xiong Jinan Univ Guangdong Prov Key Lab Pharmacodynam Constituents Inst Tradit Chinese Med & Nat Prod Coll Pharm Guangzhou Peoples R China

Three new compounds (1-3), together with six known compounds (4-9), were isolated from the ethyl acetate fraction of ethanol extract of the aerial parts of Justicia gendarussa Burm.f. All of these known compounds were isolated from the plant for the first time. Their structures including absolute configurations were elucidated on the basis of HR-ESI-MS, NMR spectroscopic analyses, single crystal X-ray diffraction and comparison with the literatures. Compounds 1-9 were tested for their antioxidant and anti-inflammatory activities. Compounds 5 and 8 showed the antioxidant activities with IC50 values of 28.61 +/- 1.56 and 22.55 +/- 1.38 mu M, respectively. Compounds 2 and 3 showed anti-inflammatory activity in LPS-induced RAW 264.7 macrophages with the IC50 values of 20.95 +/- 1.11 and 16.50 +/- 1.04 mu M, respectively.

关键词： Justicia gendarussa Burm f X-ray diffraction antioxidant activity anti-inflammatory activity

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DIffERENTIAL INHIBITION Of f0f1-ATPASE-CATALYZED REACTIONS IN BOVINE-HEART SUBMITOCHONDRIAL PARTICLES BY ORGANOTIN COMPOUNDS

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BIOCHIMICA ET BIOPHYSICA ACTA 1984年第1期766卷 209-214页

作者： EMANUEL, EL CARVER, MA SOLANI, GC GRIffITHS, DE UNIV WARWICK DEPT CHEM & MOLEC SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND INST BIOL CHEM BOLOGNA ITALY

Preincubation of coupled submitochondrial particles with low concentrations of triorganotin compounds results in complete inhibition of the oligomycin-sensitive ATPase activity without any significant effect on the rate of succinate-driven ATP synthesis. The residual ATP synthetic activity is inhibited by oligomycin and uncouplers. The differential inhibition of ATP synthesis and hydrolysis by the triorganotin compounds examined suggests that the 2 processes are not ''mirror images'' of each other, but that they occur through different routes and that the f1f0-ATPase is at least bifunctional.

关键词： f 0 f 1 -ATPase inhibition Organotin (Bovine heart) f 0 and f 1 , components of the mitochondrial ATPase system Hepes, 4-(2-hydroxyethyl)-1-piperazineethane-sulphonic acid

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Antegrade Superficial femoral Artery Approach Using Manual Compression and 4-f Delivery System for Infrainguinal Occlusive Disease

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ANNALS Of VASCULAR SURGERY 2022年第0期78卷 1-8页

作者： Nasr, Bahaa Hauguel, Alexandra Yven, Cedric Didier, Romain Gouny, Pierre Goueffic, Yann Brest Univ Hosp Dept Vasc & Endovasc Surg Brest France Paris St Joseph Hosp Grp Dept Vasc & Endovasc Surg Paris France Brest Univ Hosp Dept Cardiol Brest France

Background: This study sought to assess the feasibility and safety of antegrade superficial femoral artery (SfA) duplex-guided puncture using a 4-f catheter sheath and manual compression for the treatment of infrainguinal occlusive lesions. Methods: from March 2017 to March 2019, all patients who underwent endovascular treatment employing an antegrade SfA approach for femoropopliteal or below-the-knee lesions were included in this study. follow-up included clinical and duplex ultrasound examinations at 1 month. The primary endpoint was the frequency of major complications at the puncture site. Results: In all, 71 procedures were performed in 70 patients (46 men;mean age: 79.1 +/- 11.5 years), most of whom presented with critical limb-threatening ischemia (n = 59;83%). Of these 71 procedures, 35 (49.3%) were performed for isolated femoropopliteal lesions and 24 (33.8%) for multilevel lesions. The primary endpoint was observed in 4 patients (5.6%). There were 3 observed hematomas (4.2%): 2 required surgical treatment, and 1 necessitated transfusion without intervention. At 30 days, we reported 1 pseudoaneurysm (1.4%). Conclusions: Our results showed that antegrade SfA puncture is feasible and safe with duplex ultrasound guidance and use of a 4-f catheter sheath.

关键词： ABI ankle-brachial index CfA common femoral artery f french (unit for sheath or catheter size) PAD peripheral arterial disease SfA superficial femoral artery VCD vascular closure device

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THE GAMMA-SUBUNIT Of f1 AND THE PVP PROTEIN Of f0(f0I) ARE COMPONENTS Of THE GATE Of THE MITOCHONDRIAL f0f1H+-ATP SYNTHASE

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fEBS LETTERS 1990年第1-2期272卷 117-120页

作者： PAPA, S GUERRIERI, f ZANOTTI, f fIERMONTE, M CAPOZZA, G JIRILLO, E UNIV BARI INST IMMUNOLI-70124 BARIITALY

The γ subunit of the f 1 moiety of the bovine mitochondrial H + -ATP synthase is shown to function as a component of the gate. Addition of purified γ subunit to f o -liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis shows that the diamide effect is likely due to disulphide bridging of the γ subunit with the PVP protein of the f o sector.

关键词： H + -ATP synthase Diamide Thiol group f 1 f o Chaps, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate SDS-PAGE, sodium dodecylsulphate-polyacryl-amide gel electrophoresis USMP urea-treated submitochondrial particles DCCD, N , N '-dicyclohexylcarbodiimide NEM, N -ethyl-maleimide PVP, protein (f o I), OSCP, subunits of the membrane sector f o

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PHOTOAffINITY-LABELING Of CHLOROPLAST CYTOCHROME-B6-f COMPLEX BY AN INHIBITOR AZIDO-DERIVATIVE

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fEBS LETTERS 1983年第2期155卷 241-244页

作者： OETTMEIER, W MASSON, K OLSCHEWSKI, E Lehrstuhl Biochemie der Pflanzen Ruhr-Universität Postfach 10 21 48 4630 Bochum 1 FRG

The photoaffinity label 2-azido-2′,4,4′-trinitro-6- sec. butyldiphenylether inhibits photosynthetic electron transport in the thylakoid membrane as well as plastohydroquinone-plastocyanin-oxidoreductase activity in the isolated chloroplast cytochrome b 6 - f complex. In the latter, the 14 C-labeled inhibitor upon UV-irradiation binds covalently only to the cytochrome b 6 and the Rieske fe-S peptide subunit.

关键词： Photoaffinity label Cytochrome bin6 - f complex Inhibitor Plastoquinone Rieske fe-S protein Cytochrome b 6

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ELECTRON-MICROSCOPY Of THE RECONSTITUTED COMPLEXES Of THE f1-ATPASE WITH VARIOUS SUBUNIT CONSTITUTION REVEALED THE LOCATION Of THE GAMMA-SUBUNIT IN THE CENTRAL CAVITY Of THE MOLECULE

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fEBS LETTERS 1990年第1-2期271卷 111-115页

作者： fUJIYAMA, Y YOKOYAMA, K YOSHIDA, M WAKABAYASHI, T TOKYO INST TECHNOL DEPT LIFE SCINAGATSUTA 4259MIDORI KUYOKOHAMAKANAGAWA 227JAPAN UNIV TOKYO FAC SCIDEPT PHYSBUNKYO KUTOKYO 113JAPAN

The subunit structure of the f 1 -ATPase from the thermophilic bacterium PS3 was probed by comparing electron microscopic images of the subunit complexes reconstituted to contain different subunit compositions. The following structural features were observed. (1) The α 3 β 3 complex has a hexagonal, apparently symmetrical arrangement of masses with a central cavity. (2) The projections of the α 3 β 3 δ complexes are similar to those of the α 3 β 3 complexes. (3) In contrast, the α 3 β 3 λ complex has an additional mass in the centre which is similar to that found in the native enzyme (α 3 β 3 γδϵ). from these observations, it is concluded that the central mass in the f 1 -ATPase is comprised mostly of the γ subunit.

关键词： f 1 -ATPase Electron micrograph ATPase Thermophilic bacterium Tf 1 f 1 ATPase from the thermophilic bacterium PS3

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MEMBRANE-BOUND f420H2-DEPENDENT HETERODISULfIDE REDUCTASE IN METHANOGENIC BACTERIUM STRAIN GOL AND METHANOLOBUS-TINDARIUS

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fEBS LETTERS 1990年第1期261卷 199-203页

作者： DEPPENMEIER, U BLAUT, M MAHLMANN, A GOTTSCHALK, G UNIV GOTTINGEN INST MIKROBIOLGRISEBACHSTR 8W-3400 GOTTINGENGERMANY

Washed membrane or cytoplasmic fractions of the methanogenic bacterium strain Göl catalyzed the oxidation of coenzyme f 420 H 2 with a variety of electron acceptors. The f 420 H 2 -oxidizing activity of the cytoplasmic fraction could be assigned to a NADP + :f 420 oxidoreductase. The membrane fraction but not the cytoplasmic fraction catalyzed the oxidation of f 420 H 2 with the concomitant reduction of the heterodisulfide of 2-mercapto-ethanesulfonate and 7-mercaptoheptanoylthreonine phosphate (CoM-S-S-HTP) at a rate of 100 nmol min-mg protein according to the following equation: f 420 H 2 +CoM-S-S-HTP → f 420 + CoM + HTP-SH. The activity depended linearly on the membrane protein up to a concentration of 60 μg ml The physiological electron acceptor CoM-S-S-HTP could not be replaced by the corresponding homodisulfides CoM-S-S-CoM and HTP-S-S-HTP or by NADP + . A membrane-bound f 420 H 2 -dependent CoM-S-S-HTP reductase was also detected in Methanolobus tindarius exhibiting a specific activity of 75 nmol min m ̇ g protein. The absence of a f 420 -dependent hydrogenase in this organism excludes the involvement of this enzyme in electron transfer from H 420 H 2 to CoM-S-S-HTP.

关键词： f 420 Heterodisulfide reductase Methane formation Membrane Methanogenic bacteria CoM-SH, 2-mercaptoethanesulfonate DTNB, 5,5'-dithio- bis (2-nitrobenzoic acid) f 420 , ( N -L-lactyl-γ-L-glutamyl)-L-glutamic acid phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin-5 '-phosphate f 420 H 2 , reduced f 420 Mf, methano-furan H 4 MPT, tetrahydromethanopterin HTP-SH, 7-mercapto-heptanoylthreonine phosphate Pipes, 1,4-piperazinediethanesulfonic acid Tricine, N -[2-hydroxy-1,1- bis (hydroxymethyl)ethyl]-glycine

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EXPLORATION Of SIGMA-SUBUNIT INTERACTIONS IN BEEf-HEART MITOCHONDRIAL f1-ATPASE BY MONOCLONAL-ANTIBODIES

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fEBS LETTERS 1989年第1-2期245卷 223-228页

作者： LUNARDI, J DUPUIS, A fROBERT, Y GRASSI, J VIGNAIS, PV CENS DEPT BIOLPHARMACOL & IMMUNOL SECTF-91191 GIF SUR YVETTEFRANCE

Three monoclonal antibodies (mAbs) recognizing distinct epitopes on the δ-subunit of beef heart mitochondrial f 1 -ATPase were studied for their reactivity towards the δ-subunit both in isolated f 1 and in the f 0 -f 1 complex of submitochondrial particles. Two of the antibodies termed mAb δ 195 and mAb δ239 had free access to δ in f 1 and the f 0 -f 1 complex. Partial hindrance was observed for the third antibody mAb δ22. By a double antibinding assay, it was found that the binding sites for mAb δ195 and mAb δ239 were close to each other and possibly overlapping. Mapping studies conducted with the isolated δ-subunit showed that mAb δ195 and mAb δ239 interacted with the N-terminal portion of δ extending from Ala-1 to Met-16, whereas mAb δ22 interacted with the fragment spanning Ser-17-Glu-68. It was concluded that the Ala-1-Met-16 segment of the δ-subunit in f 1 and the f 0 -f 1 complex is freely accessible from the outside, whereas the Ser-17-Glu-68 segment of δ is partially hidden, possibly as a result of interactions with other subunits.

关键词： Monoclonal antibody ATPase, f 1 − mAb, monoclonal antibody OSCP, oligomycin sensitivity conferring protein f 1 , catalytic sector of the ATPase complex AChE, acetylcholinesterase

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MODULATION Of THE MUTAGENIC EffECTS Of 2-AMINO-3-METHYLIMIDAZO[4,5-f]QUINOLINE (IQ) AND 2-AMINO-3,4-DIMETHYLIMIDAZO[4,5-f]QUINOLINE (MEIQ) IN BACTERIA WITH RAT-LIVER 9000 X G SUPERNATANT OR MONOLAYERS Of RAT HEPATOCYTES AS AN ACTIVATION SYSTEM

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MUTATION RESEARCH 1988年第1期197卷 39-49页

作者： HOLME, JA BRUNBORG, G ALEXANDER, J TRYGG, B BJORNSTAD, C NATL INST PUBL HLTH DEPT TOXICOL GEITMYRSVEIEN 75 N-0462 OSLO 4 NORWAY

An in vitro protocol was designed to separate the process of metabolic activation from the mutational events. Cultured rat hepatocytes were first incubated with the foood mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) or 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ). After the incubation period the medium was removed and further incubated with Salmonella typhimurium TA98. A high direct mutagenic activity of the culture medium was then measured. The half-lives of the mutagenic metabolites formed form IQ and MeIQ were in the order of 45 min. The presence of the cytochrome P450 inhibitors .alpha.-naphthoflavone and metyrapone during the pre-incubation period reduced the accumulation of mutagenic metabolites. No effects of ascorbate on the mutagenic effects of IQ and MeIQ were seen. (+)-Catechin, another antioxidant and free-radical scavenger, markedly enhanced the number of IQ/MeIQ-induced revertants when added to the hepatocytes. In contrast, (+)-catechin clearly decreased the number of revertants when 9000 .times. g supernatant from rat liver (s9) was used as an activation system. No marked effect of pentachlorophenol, an inhibitor of hepatocyte sulfation and bacterial O-acetylation, was seen using hepatocytes as an activation system, while the mutagenic activity of both IQ and MeIQ was reduced by 90% in the S9/Salmonella system. The addition of an inhibitor of glucuronidation, galactosamine, or the nucleophile glutathine caused no or only minor decrease in the genotoxic effects of the IQ compounds. With both S9 and hepatocytes as activation systems the relative mutagenic effects observed in the S. typhimurium strains TA98 and TA98 NR were in the same order of magnitude, while a large decrease was seen with TA98/1,8-DNP6. The results show that this in vitro test protocol may be useful as a tool to study mechanisms involved in the formation of mutagenic metabolites.

关键词： Mutagenic activation Heterocyclic aromatic amines Hepatocytes Carcinogens Mutagens IQ MeIQ Asc ascorbate ANf α-naphthoflavone CAT (+)-catechin DMSO dimethyl sufoxide 1,8-DNP 1,8-dinitropyrene GAL galactosamine GSH glutathione HAR harmine IQ 2-amino-3-metylimidazo[4,5- f ]quinoline MeIQ 2-amino-3,4-dimethylimidazo[4,5- f ]quinoline MET metyrapone S9 9000 × g supernatant from rat-liver homogenate UDPGA uridine diphosphoglucuronic acid

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