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TEMPERATURE-SENSITIVE SYNTHESIS Of ISOCITRATE LYASE IN ESCHERICHIA COLI

BIOCHIMICA ET BIOPHYSICA ACTA 1966年第3期123卷 654-+页

作者： KORNBERG, HL SMITH, J Department of Biochemistry University of Leicester Leicester England

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Studies on the mutability of Escherichia coli K12. II. Modification of radiation sensitivity by reversions in a threonine auxotroph

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Mutation Research - fundamental and Molecular Mechanisms of Mutagenesis 1970年第2期10卷 103-109页

作者： Kada, T. Department of Induced Mutation National Institute of Genetics Mishima Japan Laboratory of Microbiology Institute of Physical and Chemical Research Saitama-ken Yamato-machi Japan

Reversions in a threonine auxotroph of E. coli K12 TH 1014 were due to suppressions involving mod and fgr loci. Large numbers of the descendants from revertant colonis showed increased sensitivities to UV irradiation or γ-rays and formed filamentous cells in the course of post-irradiation incubation. Reversions caused no modification in the host-cell reactivation capacity. These revertants were as sensitive to irradiation as E. coli B, whereas the parent auxotroph had a radiation resistance similar to that of E. coli B/r. Studies on several f^- strains carrying differently constructed mod and fgr genes indicated that the genetic character mod⁺ frg⁺ was required to manifest the marked increased radiation sensitivity accompanying filamentation/ The above observations showed an example in which common suppressor gene(s) function both in auxotrophy and filamentation. © 1970.

关键词： arg arginine f − female Hft H Hayeś male strain his histidine lac lactose leu leucine man mannose str-r resistant to streptomycin str-s sensitive to streptomycin thi thiamine thr threonine tyr trytophan

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USE Of REVERSED-PHASE COLUMN CHROMATOGRAPHY fOR RAPID ISOLATION AND IDENTIfICATION Of fORMYLMETHIONYL TRANSfER RNA

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BIOCHIMICA ET BIOPHYSICA ACTA 1969年第2期186卷 384-&页

作者： SHUGART, L CHASTAIN, B NOVELLI, GD Biology Division Oak Ridge National Laboratory Oak Ridge Tenn. 37830 U.S.A.

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The dual genetic control of ornithine transcarbamylase synthesis in Escherichia coli K12

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Mutation Research - fundamental and Molecular Mechanisms of Mutagenesis 1967年第6期4卷 743-751页

作者： Glansdorff, N. Sand, G. Verhoef, C. Laboratory of Microbiology University of Brussels Brussels Belgium Laboratory of Microbiology Utrecht Netherlands

Mutations at 2 distant genes influence ornithine transcarbamylase synthesis in Escherichia coli K12. The wild type alleles of either of them restores to an OTC-less strain the ability to synthesize this enzyme under f... 详细信息

关键词： Ade adenine arg arginine can canavanine cit citruulline his histidine lac lactose leu leucine met methionine pro proline rha rhamnose T 5 phage T 5 thi thiamine thr threonine str streptomycin Hfr, male, donor f − , female, recipient s sensitive r resistant OTC ornithinetranscarbamylase

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fRACTIONATION Of PHOTOCHEMICAL SYSTEMS Of PHOTOSYNTHESIS .2. CYTOCHROME AND CAROTENOID CONTENTS Of PARTICLES ISOLATED fROM SPINACH CHLOROPLASTS

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BIOCHIMICA ET BIOPHYSICA ACTA 1967年第1期143卷 187-+页

作者： BOARDMAN, NK ANDERSON, JM C.S.I.R.O. Division of Plant Industry Canberra Australia

Spinach chloroplasts were fragmented by incubation with digitonin, and the resulting particles examined for their cytochrome and carotenoid contents. Cytochrome difference spectra were recorded both at 20[degree]C and 77[degree]K, usually without prior extraction of the chlorophylls. It is concluded that at least three cytochromes are localized in the chloroplast;cytochromes f and bg with [alpha] bands at 554 and 563 m[mu] respectively, and another cytochrome with an a band at about 559 mu[mu] at 20[degree]C and 557 mu at 77[degree]K. The cytochromes are present in the approximate molar ratio of 1:1:2, respectively. It is assumed tentatively that cytochrome 559 (chloroplasts) is a b-type cytochrome. The chloroplast contains 430 molecules of chlorophyll per molecule of cytochrome f and 118 molecules of chlorophyll per molecule of cytochrome b (cytochrome b6 plus cytochrome 559). The results presented for the particles prepared by digitonin treatment of chloroplasts show that cytochromes f and bg are associated with Photosystem 1 and cytochrome 559 with Photosystem 2. Cytochromes f and bg are partly solubilized from the Photosystem 1 particle by the digitonin, and the method of preparation of the particles was modified to minimize this loss. Cytochrome 559 appears to be tightly bound to the Photosystem 2 particle. The four major carotenoids, [beta] -carotene, lutein, violaxanthin and neoxanthin were found to be present in both types of particles, but in varying proportions. The particles representative of Photosystem 1 were enriched in [beta] -carotene.

关键词： cyt f cytochrome f cyt b 6 cytochrome b 6 cyt b 3 cytochrome b 3 cyt 559 cytochrome 559 (chloroplasts) cyt b total cytochrome b (cyt b 6 plus cyt 559) chl a chlorophyll a chl b chlorophyll b

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The adsorption of sulphide ions at a mercury electrode

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Journal of Electroanalytical Chemistry and Interfacial Electrochemistry 1968年第2期16卷 219-238页

作者： R.D. Armstrong D.f. Porter H.R. Thirsk Department of Physical Chemistry University of Newcastle upon Tyne Newcastle upon Tyne 1 England

The impedance of a mercury electrode in sulphide ion solution of pH 9.5 has been studied using a.c. impedance techniques. Sulphide ions are very strongly specifically adsorbed at the electrode over a short potential range in the region of the e.c.m., leading to very high double-layer capacities. The potential-dependence of the capacity exhibits a maximum, followed at more anodic potentials by a discontinuity corresponding to the formation of a solid phase monolayer of HgS on the electrode. The frequency-dependence of the impedance in the potential region of adsorption indicates a relaxation process at a frequency of ≈7 kc/sec, and this is attributed to the slow dissociative adsorption of the HS − species according to the reaction scheme HS − agH + S 2− .

关键词： C Concentration of species S 1 (mole cm −3 ) C′ Concentration of species S 2 (mole cm −3 ) C 0 Concentration of species S 1 as x →∞ (mole cm −3 ) C′ 0 Concentration of species S 2 as x →∞ (mole cm −3 ) C b Series equivalent electrode capacity in base solution (μf cm −2 ) C i Differential capacity of the inner layer (μf cm −2 ) C p Parallel equivalent electrode capacity (μf cm −2 ) ∞ C p Parallel equivalent electrode capacity as ω→∞ (μf cm −2 ) C p = C p − ∞ C p (μf cm −2 ) C s Measured series capacity (μf cm −2 ) C s ( f →0) Series equivalent electrode capacity at low frequencies (μf cm −2 ) D Diffusion coefficient of species S 1 (cm 2 sec −1 ) D′ Diffusion coefficient of species S 2 (cm 2 sec −1 ) E Electrode potential (volts relative to Hg/HgO in 1 M NaOH solution) f frequency (c/sec) f faraday i Current density (A cm −2 ) j √−1 k, k′ first-order homogeneous rate constants (sec −1 ) k h first-order heterogeneous rate constant (cm sec −1 ) P Amplitude of sinusoidal perturbation (V) q m Charge in metal electrode (μC cm −2 ) q 1 Specifically adsorbed charge (μC cm −2 ) R p Parallel equivalent electrode resistance (Ω cm 2 ) R s Measured series resistance (Ω cm 2 ) R SO Series equivalent solution resistance (Ω) t Time (sec) V Rate of interfacial reaction (mole cm −2 sec −1 ) x Distance into solution from electrode (cm) Z Electrode impedance (Ω cm 2 ) Γ Concentration of ions adsorbed on electrode (mole cm −2 ) tan δ Loss tangent (=ω C s [ R s − R so [) tan δ Loss tangent (=[ω C p R p ] −1 ) μ Reaction zone thickness (cm) τ Life time of species S 1 (sec) Φ, Φ m Potential of metal electrode relative to bulk solution (V) Φ m −2 Potential of metal electrode relative to outer Helmholtz plane (V) Φ 0 D.c. compoentn of Φ (V) Φ 1 Potential of inner Helmholtz plane relative to bulk solution (V) ω Angular frequency (sec −1 ) ω Angular frequency at (tan δ ) −1 =1 (sec −1 )

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STUDIES ON ACTIN-ACTIN AND ACTIN-MYOSIN INTERACTION

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BIOCHIMICA ET BIOPHYSICA ACTA 1967年第2期140卷 208-+页

作者： BAILIN, G BARANY, M Institute for Muscle Disease New York N.Y. U.S.A.

The reagents hydrogen peroxide, iodine and l,5-difluoro-2,4-dinitrobenzene did not affect the bound nucleotide content of f-actin or G-actin at all when the viscosity of the actins and their ability to interact with myosin were greatly decreased. Separation of actin-actin interaction from actin-myosin interaction was obtained by treatment of actin with hydrogen peroxide, iodine, or iodoacetamide. Iodoacetamide reacted only with cysteine residues whereas the other reagents modified cysteine, methionine and tyrosine residues in actin. When f-actin was combined with myosin forming actomyosin, methionine and tyrosine residues of f-actin were not protected in actomyosin treated with hydrogen peroxide or iodine. However, -SH groups of f-actin were protected by myosin when actomyosin was treated with these reagents. Under these conditions, f-actin isolated from actomyosin maintained its ability to interact with myosin at high and low ionic strength. The role of -SH groups of actin in the interaction of actin and myosin is discussed. An improved method is described for the isolation of actin from actomyosin.

关键词： DfDNB 1,5-difluoro-2,4-dinitrobenzene AM f actomyosin formation AM-ATPase actin-activated myosin ATPase

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SOME PHYSICAL AND CHEMICAL PROPERTIES Of THE LIPOPROTEINS PRODUCED BY LIPOLYSIS Of HUMAN SERUM Sf20-400 LIPOPROTEINS BY POST-HEPARIN PLASMA

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JOURNAL Of ATHEROSCLEROSIS RESEARCH 1962年第1-2期2卷 104-114页

作者： SHORE, B SHORE, V Department of Physiology Washington University School of Medicine St. Louis Missouri (U.S.A.)

After human serum S f 20–400 lipoproteins were incubated with post-heparin plasma, the principal product of lipolysis was S f 11–20 (density D = 1.006–1.022 g/ml), lipoproteins. Their properties closely resembled those of molecules of comparable density among the normal human serum low density lipoproteins ( D < 1.065 g/ml). It is likely that each NH 2 -terminal amino-acid of the substrate represents the protein moiety of a lipoprotein or family of lipoproteins varying in lipid content, and that all these lipoproteins of the substrate contain triglyceride in a form susceptible to hydrolysis by post-heparin plasma. The S f 11–20 ( D = 1.006–1.022 g/ml), the S f 0–3 ( D = 1.045–1.065 g/ml), and perhaps the S f 20–400 ( D = 0.98–0.99 g/ml) lipoproteins of human serum contain molecules with the electrophoretic mobility of α 2 -globulins, whereas the S f 4–8, ( D = 1.031–1.035 g/ml) lipoproteins contain molecules with the mobility of β-globulins. Aprés incubation de lipoprotéines S f 20–400 provenant du sérum humain avec du plasma posthéparinique le produit principal de la lypolyse était des lipoprotéines S f 11–20 (densité, D = 1.006–1.022 g/ml). Leurs propriétés ressemblent bien à celles de molécules à densité comparable parmi les lipoprotéines à densité basse ( D < 1.065 g/ml) du sérum humain normal. Probablement, chaque aside aminé NH 2 -terminal du substrat représente la part des protéines d'une lipoprotéine ou d'une famille de lipoprotéines à teneur variable en lipides, et toutes ces lipoprotéines du substrat contiennent de la triglycéride sous une forme susceptible d'être hydrolysée par du plasma posthéparinique. Les lypoprotéines du sérum humain S f 11–20 (D = 1.006–1.022 g/ml), S f 0–3 ( D = 1.045–1.065 g/ml), et peut-être S f 20–400 ( D = 0.98–0.99 g/ml) contiennent des molecules ayant la mobilite électrophorétique de globulines-α 2 , tandis que les lipoprotéines S f 4–8 ( D = 1.031–1.035 g/ml) renferment des molécules ayant la mobilité de globulines-β. Nach In

关键词： S f flotation coefficient, the negative of the sedimentation coefficient in Svedberg units of species undergoing centripetal migration in a NaCl solution of density 1.0653 g/ml at 20°C LDL low density lipoproteins, lipoproteins of hydrated density less than 1.065 g/ml HDL high density lipoproteins, lipoproteins of hydrated density 1.065-1.20 g/ml. D density tris tris (hydroxymethyl)-aminomethane EDTA ethylenediamine tetraacetic acid

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MEASUREMENTS Of LINEAR POLARIZATION Of ELASTICALLY SCATTERED GAMMA RAYS

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NUCLEAR PHYSICS 1965年第3期64卷 502-&页

作者： SINGH, M SOOD, BS Physics Department Punjabi University Patiala India

Measurements are described of the linear polarization of elastically scattered gamma rays from a lead scatterrer. The results indicate that an incomplete resolution of incoherent scattering from coherent scattering tends to lower the values of the observed polarization. The measured values of the polarization of the elastic scattering of 280, 662 and 1250 keV gamma rays at different scattering angles are found to agree with the theoretical values of Brown and Mayers. In contradiction with earlier suggestions, it is found that the effect of L shell contributions is small and that it can not account for the large deviations reported previously. The present results remove the discrepancy between the experiment and theory.

关键词： ATOMIC PHYSICS Pb(γ, γ), E = 280, 662, 1250 keV measured linear polarization as f ( θ )

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PURIfICATION Of fLUORESCEIN AND fLUORESCEIN DERIVATIVES BY CELLULOSE ION EXCHANGE CHROMATOGRAPHY

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IMMUNOCHEMISTRY 1967年第4期4卷 191-+页

作者： DANDLIKER, WB ALONSO, R Division of Biochemistry Scripps Clinic and Research Foundation La Jolla California USA

A method for the purification of ionic dyes by cellulose ion exchangers is presented. The underlying rationale is based upon approximate knowledge of the pK''s of the ionizing groups of the dye. fluorescein purified by DEAE cellulose chromatography has a molar extinction coefficient of 8.72 x 104 in 0.01 [image] NaOH at 491 m[mu]. This agrees with results obtained by other workers using different methods of purification. fluorescein amine is readily purified on a 10 g scale by a similar method and an improved procedure for converting the amine to the isothiocyanate is given. The amine is a convenient and stable compound which can be standardized, stored and converted to the isothiocyanate as needed for fluorescent labeling.

关键词： f fluorescein fNO 2 nitrofluorescein fNH 2 fluorescein amine fNCS fluorescein isothiocyanate DEAE diethylaminoethyl AcOH acetic acid NH 4 OAc ammonium acetate

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