1. 1. Xanthine dehydrogenase of Drosophila melanogaster exists predominantly as one form, XDH-I, in fresh homogenates. However, a second form, XDH-II, is found in partially purified extracts. The two forms of xanthine...
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1. 1. Xanthine dehydrogenase of Drosophila melanogaster exists predominantly as one form, XDH-I, in fresh homogenates. However, a second form, XDH-II, is found in partially purified extracts. The two forms of xanthine dehydrogenase are separable on DEAE-cellulose columns and by polyacrylamide gel electrophoresis indicating that each has a different charge. XDH-I sediments slightly faster than XDH-II during sucrose gradient centrifugation indicating a small difference in molecular weight or in conformation. Other properties of the molecules are not too different. 2. 2. XDH-I can be converted to a form like XDH-II by incubation with appropriate Drosophila extracts. Preliminary analysis indicates that the converter molecule has the properties of a large protein. 3. 3. The properties of XDH-II mimic the properties of xanthine dehydrogenase from flies grown on a high protein diet. These flies also have 3- to 5-fold levels of xanthine dehydrogenase activity but no increase in xanthine dehydrogenase antigenicity (cross-reacting material against xanthine dehydrogenase antibody). It is tempting to speculate that the conversion of XDH-I to XDH-II studied in this paper represents an process in vitro related to the regulation in vivo of xanthine dehydrogenase in Drosophila.
Data are presented for the pulse-height distribution offission fragments which results from the spontaneous fission decay of the 14 ms 242 Am isomer, indicating a normal asymmetric yield-mass distribution for the fra...
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Data are presented for the pulse-height distribution offission fragments which results from the spontaneous fission decay of the 14 ms 242 Am isomer, indicating a normal asymmetric yield-mass distribution for the fragments. The partial cross section for production of the isomer by 12 MeV deuteron bombardment of 242 Pu and the spontaneous fission half-life are determined to be 8±3 μ b and 16.7±1.5 ms, respectively. A search was made for isomers which decay by spontaneous fission produced in deuteron bombardments of 232 Th, 235 U, 238 U, 239 Pu, 241 Am and 243 Am and proton bombardment of 243 Am. Upper limits are established for spontaneous fission decay with half-lives in the ranges 10–20 μ s to 1 h. A comparison is made between experimental data from deuteron bombardments of 242 Pu and the results of calculations based on the compound-statistical model.
Precision half-lives have been measured for fourteen positon-emitting nucleides. The weighted average half-lives are N 13 , 9.96±0.02 min; f 18 109.87±0.12 min; P 3c , 2.49±0.01 min; Cl 34m , 31.99±...
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Precision half-lives have been measured for fourteen positon-emitting nucleides. The weighted average half-lives are N 13 , 9.96±0.02 min; f 18 109.87±0.12 min; P 3c , 2.49±0.01 min; Cl 34m , 31.99±0.05 min; K 38 , 7.70±0.02 min; fe 53 , 8.51±0.02 min; Ni 57 , 35.99±0.12 h; Cu 62 , 9.76±0.02 min; Ga 68 , 68.33±0.09 min; Mo 91 , 15.49±0.01 min; Ag 106 , 23.96±0.04 min; Ag 108 , 2.41±0.02 min; Sb 120 , 15.89±0.04 min; and Pr 140 , 3.39±0.01 min.
An electron microscopical investigation of mitochondria from many regions of chick embryos from unincubated blastoderm through head process stages has revealed the presence of a characteristic fibrous material within ...
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An electron microscopical investigation of mitochondria from many regions of chick embryos from unincubated blastoderm through head process stages has revealed the presence of a characteristic fibrous material within the mitochondria. Various fixation techniques and electron "strains" were used, and thus far alkalinized lead acetate and uranyl acetate were particularly effective in increasing the contrast of this material. The fibers are more diffuse in stages prior to the primitive streak stage, when they become dense rods of 50-100 A diameter with some fibers extending towards the periphery of the mitochondrion from the central core. The fibrous material is characteristically observed within an area of the mitochondrial matrix which is very electron lucid. from a survey of the literature and the staining properties thus far studied it is suggested that the material may be related to deoxyribonucleic acid.
A study was made to determine the topographical organization of pallido-subthalamic fibers in the rhesus monkey. Streotaxic lesions were produced in: (1) the lateral pallidal segment (12 lesions in 10 animals), (2) th...
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A study was made to determine the topographical organization of pallido-subthalamic fibers in the rhesus monkey. Streotaxic lesions were produced in: (1) the lateral pallidal segment (12 lesions in 10 animals), (2) the medial pallidal segment (6 lesions in 6 animals), and (3) both pallidal segments (4 lesions in 4 animals). Degeneration resulting from these lesions was studied in transverse, horizontal and sagittal sections stained by the Nauta and Gygax technic14. The following conclusions were drawn from this study: 1. 1. Pallidosubthalamic fibers arising in the lateral pallidal segment are topographically organized so that: (a) rostral and central regions of this segment project fibers to the rostral two-thirds of the subthalamic nucleus, and (b) rostral parts of this segment project fibers to the medial half of the subthalamic nucleus and central parts of this segment project fibers to the lateral half of the nucleus. 2. 2. Dorsal and ventral regions of the rostral and central parts of the lateral pallidal segment are organized topographically so that: (a) fibers from dorsal regions of the rostral part terminate in lateral areas of the medial half of the subthalamic nucleus, while ventral regions project to more medial parts of this half of the nucleus, and (b) dorsal and ventral regions of the central part of this pallidal segment project to corresponding dorsal and ventral regions of the subthalamic nucleus. 3. 3. Caudal parts of the lateral pallidal segment project fibers to dorsolateral regions of the caudal part of the subthalamic nucleus. 4. 4. Observations in the present study suggest that pallidosubthalamic fibers terminating in medial and caudal parts of the subthalamic nucleus probably are derived from the medial pallidal segment. The hypothesis is presented that pallidosubthalamic fibers are topographically organized so that: (a) the most medial and lateral regions of the lateral pallidal segment project fibers respectively to medial and la
The locus of the gene for radiation sensitivity in Escherichia coli BIII has been found in crosses with several K12 Hfr''s to be closely linked to the his marker. Despite the fact that BIII, a derivative of st...
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The locus of the gene for radiation sensitivity in Escherichia coli BIII has been found in crosses with several K12 Hfr''s to be closely linked to the his marker. Despite the fact that BIII, a derivative of strain B, differs from the K12 donor in two radiation-sensitivity genes, only one type of radiation-resistant recombinant was observed, that resembling the donor parent.
Infusion of 1 mg/kg per day of prostaglandin E1 (PGE1) for 2 and 7 wk failed to correct the dermal signs of essential fatty acid (EfA) deficiency in rats despite the known conversion of EfA to certain prostaglandins. ...
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Infusion of 1 mg/kg per day of prostaglandin E1 (PGE1) for 2 and 7 wk failed to correct the dermal signs of essential fatty acid (EfA) deficiency in rats despite the known conversion of EfA to certain prostaglandins. PGE1 caused no significant changes in serum cholesterol, triglycerides, or phospholipids or in liver neutral lipids in EfA-deficient or normal rats. In normal rats epinephrine-induced lipolysis was greater in fat pads from infused than from untreated rats. The effect on epinephrine-induced lipo lysis was greater after the 7 week infusion than after the 2 week infusion. The 7 week infusion also lowered plasma free fatty acid (ffA) concentrations. Infusion of PGE2 and PGf2[alpha] in combination for 4 week had no significant effect on either dermal signs of EfA deficiency, lipo lysis, or plasma ffA concentrations.
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