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BIOLOGICAL ENERGY-PRODUCTION IN THE APPARENT ABSENCE Of ELECTRON-TRANSPORT AND SUBSTRATE LEVEL PHOSPHORYLATION

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fEBS LETTERS 1979年第1期107卷 1-3页

作者： ZEHNDER, AJB BROCK, TD Department of Bacteriology University of Wisconsin Madison WI 53706 USA

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POWER DERIVATION IN AN ANOVA MODEL WHICH IS INTERMEDIATE BETWEEN THE fIXED-EffECTS AND THE RANDOM-EffECTS MODELS

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JOURNAL Of STATISTICAL PLANNING AND INfERENCE 1979年第2期3卷 127-134页

作者： GENIZI, A SOLLER, M HEBREW UNIV JERUSALEM DEPT GENETJERUSALEM 91000ISRAEL

Some ANOVA models are presented where the “treatments effects” can assume, under the alternative, a few discrete values with prescribed probabilities. The power function, of the test that all treatment effects are zero, is approximated by a Laguerre series using the methods of Tiku (1965). Some comparisons are shown between this approximation, a direct computation and the normal approximation to the direct computation. finally, a description of some genetical problems is given where such models are appropriate and where the power calculation may improve the planning of experiments.

关键词： Non-Central f -distribution Mixture of Distributions Power Calculations

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STRUCTURE Of OXIDATIVE-PHOSPHORYLATION AND PHOTO-PHOSPHORYLATION COUPLING fACTOR COMPLEXES

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BIOCHIMICA ET BIOPHYSICA ACTA 1979年第1期549卷 31-53页

作者： BAIRD, BA HAMMES, GG CORNELL UNIV DEPT CHEM ITHACA NY 14853 USA

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ELECTROCHEMICAL STUDY Of ENERGY-DEPENDENT POTASSIUM ACCUMULATION IN ESCHERICHIA-COLI .4. REGULATION Of THE H+-K+-PUMP OPERATION BY A PERIPLASMIC PROTEIN

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BIOELECTROCHEMISTRY AND BIOENERGETICS 1979年第3期6卷 315-321页

作者： MARTIROSOV, SM Department of Biophysics Biological Faculty of Yerevan State University Yerevan USSR

The principle of regulation of the H+-K+-pump operation in E. coli cells by a specific periplasmic protein was developed. The H+-K+-pump of the gram-negative bacterium consists of 3 basic parts: ATPase located on the inside of plasma membrane f1;proteoplipid which serves as a trans-membrane ionic channel and connects f1 with periplasmic space, and finally a protein-value [PV] which associates with the exterior of fo and falls between fo and the polysaccharide portion of a cell wall. The variation in a size of periplasmic space according to cell volume can compress and strain the PV opening and blocking the outdoor of fo. Such a regulation of pump operation is actuated by osmolality in the external medium. Some effects and experimental evidence are also discussed.

关键词： Downshock will refer to a decrease and upshock to an increase in medium osmolality. f I : ATP-ase of a proton pump f o : proteolipid incorporated in a membrane PV is a protein-valve which regulates pump operation and is the third part of a pump in Gram-negative bacteria (see text) DCCD in N,N′-dicyclohexylcarbodiimide, the inhibitor of H + -pump MP - membrane potential.

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BINDING Of INULO-OLIGOSACCHARIDES TO HOMOGENEOUS IMMUNOGLOBULINS-E109 AND IMMUNOGLOBULIN-A47N

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MOLECULAR IMMUNOLOGY 1979年第2期16卷 97-100页

作者： DAS, MK STREEfKERK, DG GLAUDEMANS, CPJ NIAMDD BETHESDAMD 20014

Inulo-biose, -triose and -tetraose have been separated from, respectively, sucrose, 1-kestose and nystose by chromatography. The binding of these oligosaccharides has been studied with murine anti-inulin myeloma immunoglobulins E 109 and A47N. from these and previously obtained data it is proposed that there is a sub-site in these two anti-inulins which binds a fructose unit much more strongly than any other sub-site, and that any oligosaccharide ligand will fill that sub-site at a higher rate.

关键词： H heavy L light fruƒ fructofuranosyl fG 1 sucrose me fruƒ methyl β- d -fructofuranoside me Glc p methyl α- d glucopyranoside f 4 g 1 1 β- d -fruƒ (2[→1)-β- d -fruƒ(2] 3 →1)-α- d -Glc p Tris trishydroxymethylamino methane

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DIffERENTIAL SENSITIVITY Of ISOLATED RAT THYMOCYTES TO GLUCOCORTICOIDS fOLLOWING CONCANAVALIN-A STIMULATION

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JOURNAL Of STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 1979年第6期10卷 581-585页

作者： CIDLOWSKI, NB CIDLOWSKI, JA Department of Biochemistry University of Vermont College of Medicine Burlington VT 05405 U.S.A.

Concanavalin A (Con A) rapidly stimulates Leu incorporation into trichloroacetic acid (TCA) precipitable, but not TCA soluble material in isolated rat thymocytes. This stimulation of Leu incorporation by Con A is dose dependent, time dependent and is inhibited by the addition of cycloheximide. In non-mitogen stimulated thymocytes, cortisol (10-6 M) inhibits Leu incorporation into protein by 30%, whereas in Con A-stimulated cells, cortisol inhibits Leu incorporation by 50%. The cortisol effect in Con A-stimulated cells is present when cortisol and Con A are added simultaneously, is diminished when cortisol is added at intervals after Con A and is dependent on the concentration of cortisol administered. The inhibitory effect of cortisol on Leu incorporation is dose and time dependent in both control and Con A treated cells. The magnitude of the cortisol effect does not differ in control and Con A-stimulated thymocytes for the first 3 h of steroid exposure, after which time a significant increase in the glucocorticoid effect occurs only in the Con A-stimulated cells. This increased response to cortisol occurs at a time which is coincident with the mean maximal response of these cells to Con A alone. Both progesterone and cortexolone (10-6 M) inhibit Leu incorporation in control cells only slightly. The magnitude of these effects does not change by stimulation of the thymocytes with Con A. Under the conditions of these experiments, the rate of accumulation and the levels of nuclear glucocorticoid receptors are the same in both and Con A-stimulated thymocytes. Thymocyte responsiveness to glucocorticoid is evidently increased following exposure to Con A. This enhanced response to glucocorticoid probably occurs subsequent to nuclear accumulation of steroid receptor complexes.

关键词： f cortisol (11β 17α 21 trihydroxy-4-pregnene-3 20-dione) cortisone (17α 21-dihydroxy-4-pregnene-3 11 20 trione) corticosterone (11β 21-dihydroxy-4-pregnene-3 20 dione) cortexolone (17α 21-dihydroxy-4-pregnene-3 20-dione) progesterone (4-pregnene-3 20-dione) MEM-L minimum essential medium without leucine Con A concanavalin A

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PHOTOSYNTHESIS Of IVY LEAVES (HEDERA-HELIX L) AfTER HEAT-STRESS .3. STOMATAL BEHAVIOR

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ZEITSCHRIfT fUR PfLANZENPHYSIOLOGIE 1979年第3期92卷 277-283页

作者： BAUER, H Institut für Allgemeine Botanik der Universität Innsbruck Austria

Stomatal responses to light/dark-changes and exposure to various ambient CO 2 -concentrations of previously heat-stressed (30 min at 44, 46, or 48 °C) leaves of Hedera helix L. were studied. Stomatal conductance (k′ s ) and the intercellular CO 2 -concentration (c′ i ) were calculated from simultaneous measurements of transpiration and CO 2 -gas exchange in an open system. Stomatal responses to changes in c′ i were delayed during the first hours after heat stress and stomatal opening in light and closure in darkness were incomplete. 24 hours after stress, the stomata were able to react again as fast as before the stress, but k− s in light was still reduced. When the leaves were flushed with CO 2 poor air, however, the stomata opened almost completely. This indicates that the reduction of k− s after heat stress was caused by the raised c′ i due to reduced photosynthetic CO 2 uptake. As long as photosynthesis was impaired after heat stress, the CO 2 -sensitivity of the stomata was abnormal: related to the same c′ i , the stomata were open wider in heat-stressed leaves than in non-stressed ones.

关键词： heat stress stomatal conductance intercellular CO 2 -Concentration k a , k′ a conductance of the boundary layer for water vapour and CO 2 , respectively (cm S −1 ) k s , k′ s stomatal conductance for water vapour and CO 2 , respectively (cm s −1 ) c a , c− a concentration of water vapour and CO 2 , respectively, in the ambient air (μg cm −3 ) c i , c′ i concentration of water vapour and CO 2 , respectively, in the intercellular spaces (μg cm −3 ) Tr transpiration (μg cm −2 s −1 ) f n net photosynthesis (μg cm −2 s −1 )

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The biogenesis of rat liver mitochondrial ATPase. Subunit composition of the normal ATPase complex and of the deficient complex formed when mitochondrial protein synthesis is blocked.

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Biochimica et biophysica acta 1979年第1期548卷 48-62页

作者： de Jong L. Holtrop M. Kroon A.M. Laboratory of Physiological Chemistry State University Bloemsingel 10 9712 KZ Groningen The Netherlands

1. An ATPase complex containing 12 subunits was isoalted from rat liver mitochondria. 2. In vivo inhibition of mitochondrial protein synthesis by the chloramphenicol analogue thiamphenicol leads to the formation of an oligomycin-insensitive membrane-bound ATPase complex in mitochondria of regenerating rat liver. 3. This oligomycin-insensitive, membrane-bound ATPase was isolated by the same procedure as the ATPase complex from regenerating livers of untreated animals. 4. SDS-polyacrylamide gel electrophoresis of in vivo labelled ATPase complexes from control and from thiamphenicol-treated rats reveals that three subunits out of the 12 are not synthesized or assembled when the mitochondrial translation activity is blocked. 5. from the subunits synthesized and assembled when mitochondrial pror (fo) of the ATPase complex (subunit 5). 6. The oligomycin sensitivity-conferring protein seems absent in the ATPase complex formed in the presence of thiamphenicol.

关键词： Mitochondrial ATPase Regeneration Oligomycin-sensitive Thiamphenicol Subunit composition Protein synthesis inhibition (Rat liver) f 1 coupling factor I (soluble oligomycin-insensitive ATPase) f o coupling factor o (the membrane part of oligomycin-sensitive ATPase) f c2 coupling factor c 2 (part of f o ) OSCP oligomycin sensitivity-conferring protein (part of f o ) Hepes N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid DCCD N , N ′-dicyclohexylcarbodiimide SDS sodium dodecyl sulfate

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The amino-terminal sequence of the 40 000 molecular weight subunit of the acetylcholine receptor protein from Torpedo marmorata

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fEBS Letters 1979年第1期104卷 99-105页

作者： Devillers-Thiery, Anne Changeux, Jean-Pierre Paroutaud, Pierre Strosberg, A.Donny Neurobiologie Moléculaire and Laboratoire Associé au Centre National de la Recherche Scientifique Interactions Moléculaires et Cellulaires Paris France Unité d'Immunologie Moléculaire IRBM Université Paris VII France Biochemical Pathology Free University Brussels Belgium

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The hydroxylapatite-column assay of estrogen receptors: The routine analyses of many samples and the calculation of the equilibrium association constant

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Journal of Steroid Biochemistry 1979年第3期10卷 267-275页

作者： Erdos, T. Bessada, R. Laboratoire d'Enzymologie C.N.R.S. 91190 Gif-sur-Yvette France

The hydroxylapatite-column assay of estrogen receptors, described previously [Erdos et al. Analyt. Biochem. 37 244 (1970)], has been modified for the routine analyses of many samples. Sixty assays can be performed in 4h with the help of home-made filtering devices or even faster on commercial filter-racks. The method is at least as simple and rapid as its "batch" variant, it is particularly fitted for kinetic studies (the adsorption of protein and its elimination of hormone not bound to the receptor being very rapid) and allows for working with samples of large volume and low protein content (because protein is quantitatively and rapidly adsorbed even from very dilute solutions). The equilibrium association constant of the receptor can be calculated from data of experiments carried out by the hydroxylapatite-column method by the use of a correction factor, or directly, if the extract is diluted to contain less than 0.25 mg protein/ml. © 1979.

关键词： R uterine estradiol receptor X other estradiol-binding macromolecules than R in a crude uterine extract E estradiol [ 3 H]-E tritiated estradiol E t total E in a system E f free E E bR E bound to R e bx E bound to X E b E bound to macromolecules under equilibrium conditions (E b = E bR + E bx ) E u E unbound to R (E u = E f + E bx ) E bnonspec E bound “non specifically” to the HTP column HTP hydroxylapatite EDIAL equilibrium dialysis α the value of the ratio E bX /E f , when the latter is constant under equilibrium conditions K A equilibrium constant of association

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