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PSYCHOMETRIKA 1988年第3期53卷 393-405页

作者： GUTTMAN, L HEBREW UNIV JERUSALEM JERUSALEMISRAEL ISRAEL INST APPL SOCIAL RES JERUSALEMISRAEL

Two coefficients are proposed for measuring the extent of overlap in distributions as a direct function of the variance between the arithmetic means (“disco” and “odisco”). They are designed to answer such questions as: “Given the value of a numerical variablex, to which population should an individual be assigned so that minimum error would be incurred?” This is just the reverse of the question addressed by ANOVA. These coefficients are shown to be analytic inx and they are related to Pearson's eta and fisher'sf. Extensions of these coefficients (designed for univariate, one-way discrimination) tok-way and multivariate discriminant analysis and measurement of “interaction” are suggested.

关键词： discriminant analysis discriminant coefficients f eta monotonicity coefficients consistent statistics cumulative science ANOVA size of effect interaction

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HOT ISOSTATIC PRESSED GD2O2S-PR, CE, f TRANSLUCENT SCINTILLATOR CERAMICS fOR X-RAY COMPUTED-TOMOGRAPHY DETECTORS

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JAPANESE JOURNAL Of APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS 1988年第8期27卷 L1371-L1373页

作者： ITO, Y YAMADA, H YOSHIDA, M fUJII, H TODA, G TAKEUCHI, H TSUKUDA, Y HITACHI MET LTD MAGNET & ELECTR MAT RES LAB KUMAGAYA SAITAMA 360 JAPAN

Translucent scintillator ceramics of Gd2O2S:Pr, Ce, f were fabricated using a hot isostatic pressing (HIP) technique. The optical transmission is about 60% of the incident light and the X-ray stopping power is also quite high. Owing to the enhancement of these properties by the HIP densification process, the highest light output from a 1 mm thick specimen combined with a silicon photodiode, when excited by 120 kV X-rays, has reached 1.8 times that of CdWO4 single crystals. The present ceramics are very promising candidates for scintillator materials in X-ray computed tomography (CT) detector applications.

关键词： Ce Densificantion f Gd202S: Pr Hot isostatic pressing Scintillation property Translucent scintillator ceramic X-ray computed tomography detector

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生产力:f=f(E,K,R)

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生产力研究 1988年第5期 33-36页

作者：张践明湖南省湘潭大学哲学系

静观的生产力要素理论把生产力归结为几种要素,最通常的公式是:生产力=劳动者+生产资料+劳动对象。这种要素论在说明生产力的构成要素,在分析不同生产力发展阶段的性质方而,有着重要作用。不过这个公式带有明显的局限性。实际上,现实的... 详细信息

静观的生产力要素理论把生产力归结为几种要素,最通常的公式是:生产力=劳动者+生产资料+劳动对象。这种要素论在说明生产力的构成要素,在分析不同生产力发展阶段的性质方而,有着重要作用。不过这个公式带有明显的局限性。实际上,现实的生产力并不是这几项要素之和,人们认识到,要形成现实的生产力,必须使这些要素结合起来,没有实现结合的生产力要素,只是潜在的生产力,还不是现实的生产力。而这种理论又往往把劳动者和生产资料的结合方式唯一地作为生产资料所有制形式看待,放到生产关系中去研究。所以静观的生产力要素论没有考虑生产力要素的结合形式,它无法说明现实的生产力,当然它反

关键词：生产力要素论生产方式 E,K,R f

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fLUORESCENCE INDUCTION TRANSIENTS INDICATE DISSOCIATION Of PHOTOSYSTEM-II fROM THE PHYCOBILISOME DURING THE STATE-2 TRANSITION IN THE CYANOBACTERIUM SYNECHOCOCCUS 6301

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BIOCHIMICA ET BIOPHYSICA ACTA 1988年第1期934卷 96-107页

作者： MULLINEAUX, CW ALLEN, Jf Department of Pure and Applied Biology University of Leeds Leeds U.K.

Cells of Synechococcus 6301 were adapted to State 1 or to State 2. fluorescence induction transients on a millisecond time-scale were then recorded in the presence of DCMU, using excitation of different wavelengths primarily in order to excite either the phycobilisome or the chlorophyll a antenna of Photosystem II (PS II). The transients recorded with the phycobilisome-absorbed light indicate a reduction in PS II absorption cross-section during the transition to State 2. The transients recorded using chlorophyll a-absorbed light indicate increased energy transfer away from PS II in State 2, possibly due to increased spill-over of energy from PS II to PS I in State 2. We suggest that the basis of the State-2 transition in phycobilisome-containing organisms is a decreased excitation-energy transfer to PS II reaction centres that results from detachment of PS II from the phycobilisome.

关键词： Photosynthesis State transition Chlorophyll fluorescence induction Phycobilisome Cyanobacterium Light harvesting ( Synechococcus sp.) DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea λ ex excitation wavelength f 0 fluorescence yield when all PS II centres are open f m fluorescence yield when all PS II centres are closed LHC II light-harvesting chlorophyll a b- protein complex of PS II PS Photosystem

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MUTAGENIC AND CARCINOGENIC HETEROCYCLIC AMINES IN CHINESE COOKED fOODS

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MUTATION RESEARCH 1988年第1期201卷 181-188页

作者： ZHANG, XM WAKABAYASHI, K LIU, ZC SUGIMURA, T NAGAO, M NATL CANC CTR RES INST 1-1 TSUKIJI 5-CHOME CHUO KU TOKYO 104 JAPAN HARBIN MED UNIV SCH PUBL HLTH HARBIN PEOPLES R CHINA

Samples of 7 foods commonly eaten in the Northeast of China (i.e. fried and broiled fishes and broiled meat) were tested for mutagenicity on Salmonella typhimurium TA98 with S9 mix. The basic fractions of the samples were mutagenic, inducing 33-2930 revertants/g of cooked food. fried walleye pollack (a kind of cod fish heated on a stainless steel pan) showed the highest mutagenicity, so attempts were made to isolate mutagens from the basic fraction of this food. The mutagens were purified by treatment with blue cotton and HPLC on a semi-preparative ODS column and analytical cation exchange and ODS columns. 5 mutagens were isolated and identified as 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). 1 g of fried fish was estimated to contain 0.16 ng of IQ, 0.03 ng of MeIQ, 6.44 ng of MeIQx, 0.10 ng of 4,8-DiMeIQx and 69.2 ng of PhIP. MeIQx and PhIP accounted for 24% and 4.7%, respectively, of the total mutagenicity. The other 3 heterocyclic amines were each responsible for only 0.3-1.2% of the total mutagenicity.

关键词： Heterocyclic amines Chinese cooked foods Amines, heterocyclic AαC, 2-amino-9 H -pyrido[2,3- b ]indole, 26148-68-5 4,8-DiMeIQx, 2-amino-3,4,8-trimethylimidazol[4,5- f ]quinoxaline 5896-78-9 Glu-P-1, 2-amino-6-methyldipyrido[1,2- a ,3′,2′- d ]imidazole 67730-11-4 Glu-P-2, 2-aminodipyrido[1,2- a :3′,2′- d ]imidazole, 67730-3 IQ, 2-amino-3methylimidazo[4,5- f ]quinoline, 76180-96-6 MeAαC, 2-amino-3-methyl-9 H -pyrido[2,3- b ]indole, 68006-83-7 MeIQ, 2-amino-3,4-dimethylimidazo[4,5- f ]quinoline, 77094-11-2 MeIQx, 2-amino-3,8-dimethylimidazo[4,5- f ]quinoxaline, 77500-04-0 PhIP, 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine, 105650-23-5 Trp-P-1, 3-amino-1,4-dimethyl-5 H -pyrido[4,3- b ]indole, 62450-06-0 Trp-P-2, 3-amino-1,4-demethyl-5H-pyridol[4,3- b ]indole, 62450-07-1.

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ELECTRON-TRANSfER COMPLEXES Of ASCARIS-SUUM MUSCLE MITOCHONDRIA .3. COMPOSITION AND fUMARATE REDUCTASE-ACTIVITY Of COMPLEX-II

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BIOCHIMICA ET BIOPHYSICA ACTA 1988年第2期935卷 130-140页

作者： KITA, K TAKAMIYA, S fURUSHIMA, R MA, YC SUZUKI, H OZAWA, T OYA, H NAGOYA UNIV FAC MED DEPT BIOMED CHEM NAGOYA AICHI 464 JAPAN

Complex II of the anaerobic respiratory chain in Ascaris muscle mitochondria showed a high fumarate reductase activity when reduced methyl viologen was used as the electron donor. The maximum activity was 49 .***/min per mg protein, which is much higher than that of the mammalian counterpart. The mitochondria of Ascaris-fertilized eggs, which require oxygen for its development, also showed fumarate reductase activity with a specific activity intermediate between those of adult Ascaris and mammals. Antibody against the Ascaris flavoprotein subunit reacted with the mammalian counterparts, whereas those against the Ascaris iron-sulfur protein subunit did not crossreact, although the amino acid compositions of the subunits in Ascaris and bovine heart were quite similar. Cytochrome b-558 of Ascaris complex II was separated from flavoprotein and iron-sulphur protein subunits by high performance liquid chromatography with a gel permeation system in the presence of Sarkosyl. Isolated cytochrome b-558 is composed of two hydrophobic polypeptides with molecular masses of 17.2 and 12.5 kDa determined by gradient gel, which correspond to the two small subunits of complex II. Amino acid compositions of these small subunits showed little similarity with those of cytochrome b-560 of bovine heart complex II. NADH-fumarate reductase, which is the final enzyme complex in the anaerobic respiratory chain in Ascaris, was reconstituted with bovine heart complex I, Ascaris complex II and phospholipids. The maximum activity was 430 nmol/mg per mg protein of complex II. Rhodoquinone was essential for this reconstitution, wehreas ubiquinone showed no effect. The results clearly indicate the unique role of Ascaris complex II as fumarate reductase and the indispensability of rhodoquinone as the low-potential electron carrier in the NADH-fumarate reductase system.

关键词： Complex II fumarate reductase Cytochrome b -558 Anaerobic respirationn ( Ascaris mitochondria) f p flavoprotein subunit of complex II I p Iron-sulphur protein subunit of complex II TTfA 2-thenoyltrifluoroacetone E m ′ oxidation-reduction potential at neutral pH SDS sodium dodecyl sulfate PAGE polyacrylamide gel electrophoresis QPs a protein complex that converts succinate dehydrogenase into succinate-ubiquinone reductase HPLC high-performance liquid chromatography

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fLUORINATED ALCOHOLS AND THEIR METAL-COMPLEXES

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COORDINATION CHEMISTRY REVIEWS 1988年第C期88卷 133-202页

作者： WILLIS, CJ Department of Chemistry University of Western Ontario London N6A 5B7 Canada

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TEMPERATURE-DEPENDENCE Of GLUCOSE-TRANSPORT IN ERYTHROCYTES fROM NORMAL AND ALLOXAN-DIABETIC RATS

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BIOCHIMICA ET BIOPHYSICA ACTA 1988年第2期938卷 222-230页

作者： ABUMRAD, NA BRISCOE, P BETH, AH WHITESELL, RR VANDERBILT UNIV MED CTR SCH MED DEPT MOLEC PHYSIOL & BIOPHYS NASHVILLE TN 37232 USA

Alloxan diabetes increased 3-O-methylglucose transport rates in rat red blood cells (RBC) at temperatures below ***.C and decreased them above ***.C. Preincubation of RBC from control rats with 20 mM glucose, 3-O-methylglucose, 2-deoxyglucose or xylose greatly elevated transport at ***.C by increasing Vmax. The effect was slight at ***.C. Preincubation with glucose or deoxyglucose alone caused a 50% depression of transport rates at ***.C as a result of a rise in the Km, which is similar to findings in cells from alloxan-diabetic rats. Measurement of intracellular glucose metabolites suggested inhibition of glycolysis in cells from diabetic rats and a positive correlation between the level of intracellular hexose monophosphates and transport inhibition. Membrane fatty-acid and cholesterol composition and membrane lipid-ordering as monitored by electron paramagnetic resonance were not altered by alloxan diabetes. It is concluded that intracellular sugar and sugar metabolism alter the temperature dependence of glucose transport kinetics. Glucose metabolism can feed back to inhibit transport by increasing the transport Km at physiological temperatures only.

关键词： Glucose transport Erythrocyte Alloxan diabetes Temperature dependence (Rat) KRH Krebs-Ringer solution buffered with Hepes MeGlc 3-O- methylglucose CVdGlc 2-deoxyglucose G 6P glucose 6-phosphate f 6P fructose 6-phosphate f 1,6P 2 fructose 1,6-diphosphate GAP glyceraldehyde 3-phosphate DHAP dihydroxyacetone phosphate RBC red blood cells dGlc 6P deoxyglucose 6-phosphate

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STIMULATION Of THE CONJUGATION Of LIPID DIENES IN HEPATIC MICROSOMES BY 3,3'-DICHLOROBENZIDINE

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BIOCHEMICAL PHARMACOLOGY 1988年第5期37卷 781-791页

作者： IBA, MM LANG, B Department of Pharmacology and Toxicology Rutgers University Piscataway NJ 08854.

Pretreatment of male rats with 3,3''-dichlorobenzidine (DCB) resulted in the accumulation of conjugated dienes in lipids from hepatic microsomes. In vitro, these microsomes had 2-fold the NADPH-dependent malondialdehyde (MDA)-forming capacity of microsomes from untreated rats. To determine the mechanism of the DCB-induced accumulation of diene conjugation, the effects of added DCB on NADPH- or iron + ascorbic acid- (fe2+-ascorbate-) dependent diene conjugation, oxygen uptake and MDA formation were examined in microsomes from untreated rats in vitro. In the presence of NADPH, added DCB stimulated diene conjugation in microsomal lipids as did in vivo DCB pretreatment but inhibited the uptake of oxygen and the formation of MDA. When fe2+-ascorbate was substituted for NADPH, the formation of diene conjugation, oxygen uptake, and MDA formation were inhibited by added DCB. The DCB-induced stimulation of diene conjugation, in addition to being strictly NADPH-dependent, was carbon monoxide sensitive and was concomitant with the binding of added DCB to microsomal lipids. It is postulated that a metabolite of DCB generated by cytochrome P-450 reacts with membrane lipids both in vivo and in vitro in a manner analogous to the initiation of lipid peroxidation but at the same time prevents the autocatalytic decomposition of the lipids. The DCB-induced diene conjugation is interpreted as predisposing to deleterious changes in microsomes.

关键词： Diene conjugation is used to refer to the isomerization of double bonds from the isolated to the conjugated configuration in microsomal polyunsatur MDA, malondialdehyde TBARS, thiobarbituric acid-reactive substances (used interchangeably with MDA) fe 2+ -ascorbate, iron plus ascorbic acid 3-MC (MC), 3-methylcholanthrene ADP, adenosine diphosphate CO, carbon monoxide R f , mobility of a Chromatographic spot relative to the solvent P, microsomes. Cytochrome P-450 is used generically without reference to specific isozymes.

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THE BINDING AND RELEASE Of THE INHIBITOR PROTEIN ARE GOVERNED INDEPENDENTLY BY ATP AND MEMBRANE-POTENTIAL IN OX-HEART SUBMITOCHONDRIAL VESICLES

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BIOCHIMICA ET BIOPHYSICA ACTA 1988年第1期933卷 12-21页

作者： LIPPE, G SORGATO, MC HARRIS, DA UNIV PADUA INST BIOCHEM I-35100 PADUA ITALY UNIV PADUA CNR STUDY MITOCHONDRIAL PHYSIOL UNIT I-35100 PADUA ITALY UNIV OXFORD DEPT BIOCHEM OXFORD ENGLAND

(1) The effects of membrane potential (.DELTA..psi.) and nucleotides on the interaction between the f1-ATP synthase and its natural inhibitor protein (If1) are studied in ox-heart submitochondrial vesicles. (2) Membrane potential causes displacement of If1 from submitochondrial vesicles, as shown by measuring both .DELTA..psi.-dependent stimulation of ATPase capacity and release of 125I-labelled If1 from the vesicles. These effects are abolished if ATP is included in the incubation. (3) There is a linear increase in the steady-state ATPase capacity of oxidising vesicles as .DELTA..psi. is increased from 100 mV to 135 mV. Increasing .DELTA..psi. above 140 mV leads to no further change. (4) At a constant membrane potential, ATP suppresses the increase in ATPase capacity, with a concentration for half maximal effect of 140 .mu.M. This value is close to the Km for ATP hydrolysis by membrane-bound f1. This suppression is related to ATP concentrations rather than to .*** or ATP/ADP ratio. (5) The unidirectional on- and off-rates of If1 were measured separately. The off-rate of If1 is increased by membrane potential but unaffected by ATP. The on-rate, conversely, is increased by ATP. Thus, the suppression of the potential-dependent net release of If1 from submitochondrial vesicles by ATP results from an increase of the If1 on-rate above the off-rate.

关键词： Inhibitor protein ATP synthase ATP synthase inhibitor protein interaction ATP Membrane potential Bovine heart submitochondrial vesicle If 1 naturally occurring inhibitor protein of mitochondrial ATPase [ 125 I]If 1 radioiodinated inhibitor protein MgATP SMP submitochondrial vesicles prepared in the presence of ATP and magnesium [ 125 I]If 1 SMP submitochondrial vesicles supplemented with [ 125 I]If 1 Δp protonmotive force = Δ∼μ H f = Δψ + (2.303 RT f ) ΔpH , where Δψ is the electrical component and ΔpH the chemical component of the transmembrane difference of the electrochemical potential of protons ( Δ ∼ μ H ) P i inorganic phosphate ΔG P = ΔG O′ + RT In ( [ATP] [ADP][P i ] ) Ap 5 A, P 1 ,P 5 -di(adenosine-5′-)pentaphosphate fCCP carbonylcyanide p -trifluoromethoxyphenylhydrazone CCCP carbonylcyanide m -chlorophenylhydrazone

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