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PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS 1984年第5-6期8卷 305-313页

作者： BORNMAN, Jf BJORN, LO AKERLUND, HE UNIV LUND DEPT BIOCHEMS-22007 LUND 7SWEDEN

The effect of ultraviolet (UV) radiation (half-band width 10 nm) in the wavelength range 248-340 nm on chlorophyll fluorescence from a thin layer of spinach thylakoid suspension was investigated. It was found that the parameter most sensitive to UV radiation was the rise time of variable fluorescence. The increase in rise time was proportional to UV photon fluence and was used for the determination of an action spectrum. The action spectrum falls off from a maximum at ca. 275 nm towards longer wavelengths and rises from a minimum at 260 nm towards shorter wavelengths. The results also suggest that the UV inhibition is mainly on the PS II oxidizing side. Possibly damage is also inflicted to the PS II reaction center.

关键词： photosynthesis inhibition Spinacia oleracea UV-B UV-C UV inhibition variable fluorescence Chl chlorophyll DCMU 3-(3.4-dichlorophenyl)-1, 1-dimethylurea f max maximum fluorescence f 0 initial fluorescence f v variable fluorescence PQ plastoquinone PS I and II photosystems I and II Q quencher of fluorescence/a primary electron acceptor of PS II UV-B ultraviolet-B radiation (280-320 nm) UV-C uhraviolet-C radiation (< 280 nm)

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GLUCOCORTICOID RECEPTOR NUMBER AND INTRACELLULAR WATER SPACE

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LEUKEMIA RESEARCH 1984年第1期8卷 87-92页

作者： JUNKER, K RIGHOSP DEPT NUCL MED DK-2100 COPENHAGEN DENMARK

To elucidate the relationship between cell water content and number of glucocorticoid receptors, 11 normal and malignant lymphoid or myelomonocytic cell types originating from mouse, rat and man were investigated. Cellular water space was measured with 3H2O and glucocorticoid receptor number was measured in a whole-cell binding assay with [3H]dexamethasone at ***. and ***. C. Intracellular water phase concentration of glucocorticoid receptors (around 40 nmol/l cell water), and the dependence of receptor affinity on temperature were similar in normal and malignant rodent and human cells. Comparisons of glucocorticoid receptor levels are best made on the basis of intracellular receptor concentrations.

关键词： Glucocorticoid receptor dexamethasone leukemia-lymphoma cell lines Dexamethasone 9α-fluoro-11β 17α, 21-trihydroxy-16α-methyl-pregna-1, 4-diene-3,20-dione [Dex] concentration of free labelled plus unlabelled dexamethasone PBS phosphate-buffered saline K d dissociation constant of dexamethasone from its receptor B radioactivity of cell pellet from 300 μl cell suspension f radioactivity of 150 μl supernatant B/f nonspec nonspecifically bound fraction of dexamethasone

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MMPI SUBTYPES AND ANTISOCIAL BEHAVIORS IN ADOLESCENT ALCOHOL AND DRUG-ABUSERS

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DRUG AND ALCOHOL DEPENDENCE 1984年第3期13卷 235-244页

作者： SUTKER, PB MOAN, CE GOIST, KC ALLAIN, AN ORLEANS CLIN PSYCHOL NEW ORLEANS LA 70115 USA TULANE UNIV SCH MED DEPT PSYCHIAT & NEUROL NEW ORLEANS LA 70112 USA

Relationships between psychopathology and alcohol, drug abuse and other deviant behaviors were studied in a sample of 60 adolescents with exaggerated drug use patterns. Modal Minnesota Multiphasic Personality Inventory (MMPI) profile subtypes were identified using multivariate analyses, and adolescents defined by profile type were compared on background and antisocial history variables derived from retrospective self-reports. Data were collected reflecting a variety of drug categories, including alcohol and opiates. Three distinct patterns were found, with 97% of adolescents meeting criteria for profile type assignment. Adolescent subtypes were characterized by significant differences in degree and type of psychopathology but not in antisocial features. Adolescent alcohol and illicit drug use were associated with marked antisocial features, but the more exclusively antisocial drug-using adolescents tended to be involved in earlier and more extensive types of alcohol and drug activities.

关键词： Adolescence Alcohol and drug abuse Antisocial characteristics Minnesota Multiphasic Personality Inventory D Depression f Validity Hs Hypochondriasis . Hy Hysteria K Correction L Lie Ma Hypomania MAC MacAndrew Alcoholism Scale Mf Masculinity-feminity MMPI Minnesota Multiphasic Personality Inventory Pa Paranoia Pd Psychopathic Deviate Pt Psychasthenia Sc Schizophrenia Si Social Introversion

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CATION REQUIREMENT fOR THE RECONSTITUTION Of OLIGOMYCIN-SENSITIVE ATPASE BY MEANS Of SOLUBLE f1-ATPASE AND f-1-DEPLETED SUBMITOCHONDRIAL PARTICLES

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第1期723卷 1-6页

作者： SANDRI, G SURANYI, E ERIKSSON, LEG WESTMAN, J ERNSTER, L UNIV STOCKHOLM ARRHENIUS LAB DEPT BIOCHEM S-10691 STOCKHOLM SWEDEN UNIV STOCKHOLM ARRHENIUS LAB DEPT BIOPHYS S-10691 STOCKHOLM SWEDEN

Bovine heart submitochondrial particles depleted of f1 by treatment with urea (f1-depleted particles) were incubated with soluble f1-ATPase. The binding of f1 to the particles and the concomitant conferral of oligomycin sensitivity on the ATPase activity required the presence of cations in the incubation medium. NH4+, K+, Rb+, Cs+, Na+ and Li+ promoted reconstitution maximally at 40-74 mM, guanidinium+ and Tris+ at 20-30 mM, and Ca2+ and Mg2+ at 3-5 mM. The particles exhibited a negative .zeta.-potential, as determined by microelectrophoresis, and this was neutralized by mono- and divalent cations in the same concentration range as that needed to promote f1 binding and reconstituion of oligomycin-sensitive ATPase. The cations act by neutralizing negative charges on the membrane surface, mainly negatively charged phospholipids. These results are discussed in relation to earlier findings reported in the literature with f1-depleted thylakoid membranes and with submitochondrial particles depleted of both f1 and the coupling proteins f6 and oligomycin sensitivity-conferring protein.

关键词： Cation requirement ATPase reconstitution f 1 -ATPase (Submitochondrial particle) f 1 coupling factor 1 f 6 coupling factor 6

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INTERACTION Of f1-ATPASE, fROM OX HEART-MITOCHONDRIA WITH ITS NATURALLY-OCCURRING INHIBITOR PROTEIN - STUDIES USING RADIOIODINATED INHIBITOR PROTEIN

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第1期724卷 128-141页

作者： POWER, J CROSS, RL HARRIS, DA UNIV LEEDS DEPT BIOCHEM LEEDS LS2 9JT W YORKSHIRE ENGLAND SUNY UPSTATE MED CTR SYRACUSE NY 13210 USA

The ox heart mitochondrial inhibitor protein may be iodinated with up to 0.8 mol 125I/mol inhibitor with no loss of inhibitory activity, with no change in binding affinity to submitochondrial particles and without alteration in the response of membrane-bound inhibitor to energization. Tryptic peptide maps reveal a single labeled peptide, consistent with modification of the single tyrosine residue of the protein. A single type of high-affinity binding site (Kd = 96 .cntdot. 10-9 M) for the inhibitor protein was measured in submitochondrial particles. The concentration of this site is proportional to the amount of membrane-bound f1, and there appears to be 1 such site per f1 molecule. The ATP hydrolytic activity of submitochondrial particles is inversely proportional to the occupancy of the high-affinity binding site for the inhibitor protein. No evidence is found for a non-inhibitory binding site on the membrane or on other mitochondrial proteins. In intact mitochondria from bovine heart, the inhibitor protein is present in an .apprx. 1:1 ratio with f1. Submitochondrial particles prepared by sonication of these mitochondria with MgATP contain about 0.75 mol inhibitor protein per mol f1, and show about 25% of the ATPase activity of inhibitor-free submitochondrial particles. Additional inhibitor protein can be bound to these particles to a level of 0.2 mol/mol f1, with consequent loss of ATPase activity. If MgATP is omitted from the medium, or inhibitors of ATP hydrolysis are present, the rate of combination between f1 and its inhibitor protein is very much reduced. The equilibrium level of binding is, however, unaltered. These results suggest the presence of a single, high-affinity, inhibitory binding site for inhibitor protein on membrane-bound f1. The energization of coupled submitochondrial particles by succinate oxidation or by ATP hydrolysis results in both the dissociation of inhibitor protein into solution, and the activation of ATP hydrolysis. At least 80% of

关键词： f 1 -ATPase Inhibitor protein Enzyme-inhibitor interaction, (Bovine heart mitochondria) Ado PP [NH] P adenosine 5′-[β, γ-imido]triphosphate fCCP carbonyl cyanide p -trifluoromethoxy-phenylhydrazone Hepes N -2-hydroxyethylpiperazine- N ′-2-ethanesulphonic acid Nbf-Cl 4-chloro 7-nitrobenzofurazan Mops 3-( N -morpholino)propanesulphonic acid MgATP particles phosphorylating particles prepared by sonication of ox heart mitochondria in the presence of 15 mM MgCl 2 and 1 mM ATP state III particles MgATP particles treated by sedimentation in the presence of 5 mM succinate, 10 mM potassium phosphate, 5 mM MgCl 2 and 1 mM ADP (pH 7.4 with KOH) AS particles non-phosphorylating particles prepared by sonication of ox heart mitochondria in the presence of 2 mM EDTA (pH 9.2 with ammonia), followed by passage through a Sephadex G50 column

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PROTEINS CONTAINING THE fACTOR-f430 fROM METHANOSARCINA-BARKERI AND METHANOBACTERIUM-THERMOAUTOTROPHICUM - ISOLATION AND PROPERTIES

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第1期742卷 84-90页

作者： MOURA, I MOURA, JJG SANTOS, H XAVIER, AV BURCH, G PECK, HD LEGALL, J INST SUPER TECN CTR QUIM ESTRUTURAL COMPLEXO I AVE ROVISCO PAIS P-1000 Lisbon PORTUGAL UNIV MINNESOTA GRAY FRESHWATER BIOL INST NAVARRE MN 55392 USA UNIV GEORGIA DEPT BIOCHEM ATHENS GA 30602 USA

for the 1st time, the unique Ni-containing cofactor (compound f430) has been shown to exist in a protein-bound form. The proteins isolated from Methanosarcina barkeri and Methanobacterium thermoautotrophicum were designated P425''(445'') on the basis of their spectral characteristics. The proteins contained 3 different subunits and had MW of .apprx. 300,000. Heat treatment or acidification of the proteins releases an f430 cofactor identical to the one isolated in Methanobacterium bryantii and Methanobacterium thermoautotrophicum.

关键词： Methanogenic bacterium f 430 Nickel protein

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A COMPACT f-SPACE NOT CO-ABSOLUTE WITH BETA-N-N

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TOPOLOGY AND ITS APPLICATIONS 1983年第1期15卷 59-64页

作者： VANMILL, J WILLIAMS, SW SUNY BUFFALO BUFFALONY 14214

We show that if every Parovičenko space of weight c is co-absolute with β N − N , then c <2 ℵ 1 .

关键词： 54G05, 54D40, 54A35, 06E99 f -space absolute β N − N Boolean alegbra Continuum Hypothesis

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MECHANISM Of THE LIGHT STATE TRANSITION IN PHOTOSYNTHESIS .1. ANALYSIS Of THE KINETICS Of CYTOCHROME-f OXIDATION IN STATE-1 AND STATE-2 IN THE RED ALGA, PORPHYRIDIUM-CRUENTUM

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第1期724卷 111-117页

作者： BIGGINS, J BROWN UNIV DIV BIOL & MED PROVIDENCE RI 02912 USA

The kinetics of photooxidation and reduction of cytochrome [cyt] f were examined spectrophotometrically in the red alga P. cruentum in light state 1 and light state 2. Experiments were performed on intact cells that had been chemically fixed and stabilized in the light states. The cyt f turnover was measured during conditions of linear electron transport driven by both photosystems and during several cyclic reactions mediated by the long-wavelength photosystem (PS) I. The rate of photooxidation of cyt f increased in state 2 when the cells were activated by subsaturating intensities of green light absorbed primarily by the phycobilisome. No differences in kinetics were found between algae in state 1 or state 2 when they were activated by light absorbed primarily by the chlorophyll of PS I. Changes in energy distribution between the 2 photosystems occur as a result of the light state transition and verify that the redistribution of excitation results in the predicted changes in electron transport.

关键词： Cytochrome f oxidation Photosynthesis Light state transition Electron transport fluorescence (Porphyridium cruentum) PS photosystem LHC light-harvesting chlorophyll a b protein DCMU 2-(3,4-dichlorophenyl)-1,1-dimethylurea TMPD N , N , N ′, N ′-tetramethyl- p -phenylenediamine DCIP 2,6-dichlorophenolindophenol

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INTERACTION Of ESCHERICHIA-COLI f1-ATPASE WITH DICYCLOHEXYLCARBODIIMIDE-BINDING POLYPEPTIDE

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第2期733卷 274-282页

作者： LOO, TW STANLOTTER, H MACKENZIE, D MOLDAY, RS BRAGG, PD UNIV BRITISH COLUMBIA DEPT BIOCHEM VANCOUVER V6T 1W5 BC CANADA

Antibody raised against the N,N''-dicyclohexylcarbodiimide (DCCD)-binding polypeptide of E. coli bound to the cytoplasmic surface of the cell membrane. A weak reaction was seen with everted vesicles of the thermophile PS3. Rat liver mitochondrial membranes did not react with the antibody. Reaction of the isolated DCCD-binding polypeptide with the antibody was prevented by oxidation of methionine residues or cleavage of the polypeptide with CNB. Modification of the arginine residues of the DCCD-binding polypeptide did not affect interaction with the antibody. Purified f1-ATPase of E. coli bound to the isolated DCCD-binding polypeptide as shown by solid-phase radioimmune assay. Binding involved the .alpha. and/or .beta. subunits of f1 and the arginine residues of the polar central region of the DCCD-binding polypeptide. The results are consistent with a looped arrangement of the DCCD-binding polypeptide in the membrane in which the carboxyl- and amino-terminal regions of the molecule are at the periplasmic surface and the polar central region, interacting with f1, is at the cytoplasmic surface of the cell membrane.

关键词： f 1 -ATPase Dicyclohexylcarbodiimide-binding polypeptide Protein orientation ( E. coli ) DCCD N,N 1 - dicyclohexylcarbodiimide f 1 f 1 -ATPase protein of the ATPase complex f 0 f 0 protein of the ATPase complex

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IMMUNOCHEMICAL ANALYSIS Of MICROCOCCUS-LYSODEIKTICUS (LUTEUS) f1-ATPASE AND ITS SUBUNITS

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BIOCHIMICA ET BIOPHYSICA ACTA 1983年第2期724卷 230-240页

作者： URBAN, C SALTON, MRJ NYU MED DEPT MICROBIOL NEW YORK NY 10016 USA

The f1-ATPase from M. lysodeikticus [M. luteus] was purified to 95% homogeneity;as do all other bacterial f1-ATPases, it possesses 5 distinct subunits with MW ranging from 60,000-10,000. The immunochemical reactivities of antibodies to native and sodium dodecyl sulfate (SDS)-dissociated subunits with the native and dissociated f1-ATPase were studied. Antibodies generated to native or SDS-dissociated subunits reacted with the native molecule. All of the subunits were antigenically unique by crossed immunoelectrophoresis and Ouchterlony double-diffusion. Antibodies to SDS-denatured individual .delta.- and .epsilon.-subunits destabilized the interaction of these subunits with the rest of the native f. All subunit antibodies and anti-native f1 inhibited ATPase activity to varying degrees, the strongest inhibition being seen with antibodies to the total f1 and anti-.alpha.- and anti-.beta.-subunit antibodies. The interaction of specific subunit antibodies may provide a new way to study the catalytic portions of f1-ATPases and may offer an additional method for the examination of multimeric proteins.

关键词： f 1 -ATPase Immunoelectrophoresis (Micrococcus lysodeikticus)

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