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IMMUNOLOGICAL AND RECONSTITUTION STUDIES ON THE ADENOSINE-TRIPHOSPHATASE COMPLEX fROM RHODOSPIRILLUM-RUBRUM

BIOCHIMICA ET BIOPHYSICA ACTA 1979年第1期545卷 77-85页

作者： MULLER, HW SCHMITT, M SCHNEIDER, E DOSE, K UNIV MAINZ INST BIOCHEMD-6500 MAINZFED REP GER

Studies on restoration of membrane-bound adenosinetriphosphatase (ATP phosphohydrolase, EC 3.6.1.3) from Rhodospirillum rubrum show that the δ-subunit is capable of binding to the f 1 factor or to the f 0 moiety of the f 0 -f 1 ATPase complex. This subunit is thus likely involved in linking the f 0 and f 1 factor. During solubilization of the oligomycin-sensitive f 0 -f 1 ATPase complex with Triton X-100 the detergent becomes specifically associated with the lipophilic f 0 part of the enzyme complex. Crossed immunoelectrophoresis, agglutination tests, and kinetic studies with anti-f 1 ATPase antibodies reveal a reaction of immunological identity of membrane-bound ATPase, f 0 -f 1 ATPase, and f 1 ATPase.

关键词： ATPase complex f 1 -ATPase Reconstitution (Rhodospirillum rubrum)

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STRUCTURE Of OXIDATIVE-PHOSPHORYLATION AND PHOTO-PHOSPHORYLATION COUPLING fACTOR COMPLEXES

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BIOCHIMICA ET BIOPHYSICA ACTA 1979年第1期549卷 31-53页

作者： BAIRD, BA HAMMES, GG CORNELL UNIV DEPT CHEM ITHACA NY 14853 USA

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INACTIVE TO ACTIVE TRANSITIONS Of THE MITOCHONDRIAL ATPASE COMPLEX AS CONTROLLED BY THE ATPASE INHIBITOR

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BIOCHIMICA ET BIOPHYSICA ACTA 1979年第2期547卷 252-257页

作者： GOMEZPUYOU, A DEGOMEZPUYOU, MT ERNSTER, L UNIV STOCKHOLM DEPT BIOCHEM ARRHENIUS LAB S-10691 STOCKHOLM SWEDEN

The hydrolytic and phosphorylation activities of the ATPase complex of bovine heart mitochondria are regulated by the ATPase inhibitor of Pullman and Monroy. The inhibiting action of the peptide on ATPase activity was overcome by a proton-motive force. Submitochondrial particles that contain the inhibitor, either intrinsically or externally added, show a lag that precedes phosphorylation. Particles devoid of the inhibitor or particles that are in an active state fail to present the lag. Prior to the onset of phosphorylation, the ATPase complex probably undergoes a transition to an active state through a process that involves the inhibitor. The transition depends on the concentration of ATP, 50 .mu.M ATP giving 50% inhibition of the proton-motive force-induced transition.

关键词： ATPase ATPase inhibitor Mitochondria Proton-motive force Submitochondrial particle f 1 -ATPase soluble mitochondrial ATPase fCCP carbonylcyanide p -trifluoromethoxy-phenylhydrazone MES 2-( N -morpholino)ethanesulfonic acid

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POWER DERIVATION IN AN ANOVA MODEL WHICH IS INTERMEDIATE BETWEEN THE fIXED-EffECTS AND THE RANDOM-EffECTS MODELS

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JOURNAL Of STATISTICAL PLANNING AND INfERENCE 1979年第2期3卷 127-134页

作者： GENIZI, A SOLLER, M HEBREW UNIV JERUSALEM DEPT GENETJERUSALEM 91000ISRAEL

Some ANOVA models are presented where the “treatments effects” can assume, under the alternative, a few discrete values with prescribed probabilities. The power function, of the test that all treatment effects are zero, is approximated by a Laguerre series using the methods of Tiku (1965). Some comparisons are shown between this approximation, a direct computation and the normal approximation to the direct computation. finally, a description of some genetical problems is given where such models are appropriate and where the power calculation may improve the planning of experiments.

关键词： Non-Central f -distribution Mixture of Distributions Power Calculations

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ELECTROCHEMICAL STUDY Of ENERGY-DEPENDENT POTASSIUM ACCUMULATION IN ESCHERICHIA-COLI .4. REGULATION Of THE H+-K+-PUMP OPERATION BY A PERIPLASMIC PROTEIN

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BIOELECTROCHEMISTRY AND BIOENERGETICS 1979年第3期6卷 315-321页

作者： MARTIROSOV, SM Department of Biophysics Biological Faculty of Yerevan State University Yerevan USSR

The principle of regulation of the H+-K+-pump operation in E. coli cells by a specific periplasmic protein was developed. The H+-K+-pump of the gram-negative bacterium consists of 3 basic parts: ATPase located on the inside of plasma membrane f1;proteoplipid which serves as a trans-membrane ionic channel and connects f1 with periplasmic space, and finally a protein-value [PV] which associates with the exterior of fo and falls between fo and the polysaccharide portion of a cell wall. The variation in a size of periplasmic space according to cell volume can compress and strain the PV opening and blocking the outdoor of fo. Such a regulation of pump operation is actuated by osmolality in the external medium. Some effects and experimental evidence are also discussed.

关键词： Downshock will refer to a decrease and upshock to an increase in medium osmolality. f I : ATP-ase of a proton pump f o : proteolipid incorporated in a membrane PV is a protein-valve which regulates pump operation and is the third part of a pump in Gram-negative bacteria (see text) DCCD in N,N′-dicyclohexylcarbodiimide, the inhibitor of H + -pump MP - membrane potential.

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INSITU PHOSPHORYLATION Of THE ALPHA-SUBUNIT Of EUKARYOTIC INITIATION fACTOR-2 IN RETICULOCYTE LYSATES INHIBITED BY HEME DEfICIENCY, DOUBLE-STRANDED-RNA, OXIDIZED GLUTATHIONE, OR THE HEME-REGULATED PROTEIN-KINASE

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PROCEEDINGS Of THE NATIONAL ACADEMY Of SCIENCES Of THE UNITED STATES Of AMERICA 1979年第5期76卷 2118-2122页

作者： ERNST, V LEVIN, DH LONDON, IM HARVARD UNIV DEPT MED BOSTON MA 02115 USA

Protein synthesis initiation in [rabbit] reticulocyte lysates was inhibited by heme deficiency, low levels of double-stranded RNA (dsRNA), oxidized glutathione (GSSG) or the purified kinase (HRI) that acts on the .alpha. polypeptide of eukaryotic initiation factor 2 (***.). The phosphoprotein profiles produced in lysates in response to these various conditions were monitored directly in lysates after labeling for brief periods with pulses of [.gamma.-32P]ATP. The [32P]phosphoprotein profiles were analyzed by electrophoresis in sodium dodecyl sulfate/polyacrylamide slab gels under conditions where the HRI and ***. polypeptides were clearly distinguished. All 4 modes of inhibition produced a rapid phosphorylation of ***. compared to control lysates, which displayed little or no phosphorylation of ***.. In heme-deficient lysates, phosphorylation of ***. aoccurred rapidly before and after the shut-off of protein synthesis. The delayed addition of hemin to these lysates resulted in a decrease in the phosphorylation of ***. and the subsequent restoration of protein synthesis. Rapid turnover of phosphate apparently occurs at the site(s) of ***. phosphorylation. In lysates inhibited by heme deficiency, GSSG or added HRI, the phosphorylation of ***. was accompanied by the rapid in situ phosphorylation of HRI. The inhibition of initiation induced by dsRNA was accompanied by the phosphorylation of ***. and a 67,000 dalton polypeptide but not HRI. These observations in situ indicate that the phosphorylation of ***. is the critical event in these inhibitions of protein chain initiation, and the phosphorylation of HRI is associated with its activation in heme deficiency.

关键词： protein synthesis regulation Met-tRNA f Met binding factor inhibition of initiation [ 32 P]phosphoprotein profiles

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INHIBITION Of INSULIN RECEPTOR-BINDING BY DIMETHYL-SULfOXIDE

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BIOCHIMICA ET BIOPHYSICA ACTA 1979年第3期582卷 486-495页

作者： VANOBBERGHEN, E DEMEYTS, P ROTH, J Diabetes Branch National Institute of Arthritis Metabolism and Digestive Diseases National Institutes of Health Bethesda MD 20014 U.S.A.

Little is known of the effects of the solvent on hormone-receptor interactions. In the present study the effect of the polar solvent dimethyl sulfoxide on the binding of insulin to its surface receptors on cultured human lymphocytes of the IM-9 line was investigated. At concentrations exceeding 0.1% (v/v), dimethyl sulfoxide produced a dose-related inhibition of 125 I-labeled insulin binding. Insulin binding was totally abolished in 20% dimethyl sulfoxide. This inhibition was immediately present and was totally reversible. Analysis of the data of binding at steady state indicated that the decrease in binding of 125 I-labeled insulin was due to a reduced affinity of the insulin receptor without noticeable change in the concentration of receptor sites. Kinetic studies showed that the decreased affinity could largely be accounted for by a decreased association rate constant; effects on dissociation and negative cooperativity of the insulin receptor were affected to a much lesser extent.

关键词： Insulin Receptor binding Dimethyl sulfoxide (Negative cooperativity) Me 2 SO, dimethyl sulfoxide, HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid B / f bound/free R 0 total concentration of receptor sites per cell K average affinity K e limiting high affinity state K f limiting low affinity state

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BINDING Of INULO-OLIGOSACCHARIDES TO HOMOGENEOUS IMMUNOGLOBULINS-E109 AND IMMUNOGLOBULIN-A47N

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MOLECULAR IMMUNOLOGY 1979年第2期16卷 97-100页

作者： DAS, MK STREEfKERK, DG GLAUDEMANS, CPJ NIAMDD BETHESDAMD 20014

Inulo-biose, -triose and -tetraose have been separated from, respectively, sucrose, 1-kestose and nystose by chromatography. The binding of these oligosaccharides has been studied with murine anti-inulin myeloma immunoglobulins E 109 and A47N. from these and previously obtained data it is proposed that there is a sub-site in these two anti-inulins which binds a fructose unit much more strongly than any other sub-site, and that any oligosaccharide ligand will fill that sub-site at a higher rate.

关键词： H heavy L light fruƒ fructofuranosyl fG 1 sucrose me fruƒ methyl β- d -fructofuranoside me Glc p methyl α- d glucopyranoside f 4 g 1 1 β- d -fruƒ (2[→1)-β- d -fruƒ(2] 3 →1)-α- d -Glc p Tris trishydroxymethylamino methane

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DIffERENTIAL SENSITIVITY Of ISOLATED RAT THYMOCYTES TO GLUCOCORTICOIDS fOLLOWING CONCANAVALIN-A STIMULATION

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JOURNAL Of STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 1979年第6期10卷 581-585页

作者： CIDLOWSKI, NB CIDLOWSKI, JA Department of Biochemistry University of Vermont College of Medicine Burlington VT 05405 U.S.A.

Concanavalin A (Con A) rapidly stimulates Leu incorporation into trichloroacetic acid (TCA) precipitable, but not TCA soluble material in isolated rat thymocytes. This stimulation of Leu incorporation by Con A is dose dependent, time dependent and is inhibited by the addition of cycloheximide. In non-mitogen stimulated thymocytes, cortisol (10-6 M) inhibits Leu incorporation into protein by 30%, whereas in Con A-stimulated cells, cortisol inhibits Leu incorporation by 50%. The cortisol effect in Con A-stimulated cells is present when cortisol and Con A are added simultaneously, is diminished when cortisol is added at intervals after Con A and is dependent on the concentration of cortisol administered. The inhibitory effect of cortisol on Leu incorporation is dose and time dependent in both control and Con A treated cells. The magnitude of the cortisol effect does not differ in control and Con A-stimulated thymocytes for the first 3 h of steroid exposure, after which time a significant increase in the glucocorticoid effect occurs only in the Con A-stimulated cells. This increased response to cortisol occurs at a time which is coincident with the mean maximal response of these cells to Con A alone. Both progesterone and cortexolone (10-6 M) inhibit Leu incorporation in control cells only slightly. The magnitude of these effects does not change by stimulation of the thymocytes with Con A. Under the conditions of these experiments, the rate of accumulation and the levels of nuclear glucocorticoid receptors are the same in both and Con A-stimulated thymocytes. Thymocyte responsiveness to glucocorticoid is evidently increased following exposure to Con A. This enhanced response to glucocorticoid probably occurs subsequent to nuclear accumulation of steroid receptor complexes.

关键词： f cortisol (11β 17α 21 trihydroxy-4-pregnene-3 20-dione) cortisone (17α 21-dihydroxy-4-pregnene-3 11 20 trione) corticosterone (11β 21-dihydroxy-4-pregnene-3 20 dione) cortexolone (17α 21-dihydroxy-4-pregnene-3 20-dione) progesterone (4-pregnene-3 20-dione) MEM-L minimum essential medium without leucine Con A concanavalin A

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PHOTOSYNTHESIS Of IVY LEAVES (HEDERA-HELIX L) AfTER HEAT-STRESS .3. STOMATAL BEHAVIOR

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ZEITSCHRIfT fUR PfLANZENPHYSIOLOGIE 1979年第3期92卷 277-283页

作者： BAUER, H Institut für Allgemeine Botanik der Universität Innsbruck Austria

Stomatal responses to light/dark-changes and exposure to various ambient CO 2 -concentrations of previously heat-stressed (30 min at 44, 46, or 48 °C) leaves of Hedera helix L. were studied. Stomatal conductance (k′ s ) and the intercellular CO 2 -concentration (c′ i ) were calculated from simultaneous measurements of transpiration and CO 2 -gas exchange in an open system. Stomatal responses to changes in c′ i were delayed during the first hours after heat stress and stomatal opening in light and closure in darkness were incomplete. 24 hours after stress, the stomata were able to react again as fast as before the stress, but k− s in light was still reduced. When the leaves were flushed with CO 2 poor air, however, the stomata opened almost completely. This indicates that the reduction of k− s after heat stress was caused by the raised c′ i due to reduced photosynthetic CO 2 uptake. As long as photosynthesis was impaired after heat stress, the CO 2 -sensitivity of the stomata was abnormal: related to the same c′ i , the stomata were open wider in heat-stressed leaves than in non-stressed ones.

关键词： heat stress stomatal conductance intercellular CO 2 -Concentration k a , k′ a conductance of the boundary layer for water vapour and CO 2 , respectively (cm S −1 ) k s , k′ s stomatal conductance for water vapour and CO 2 , respectively (cm s −1 ) c a , c− a concentration of water vapour and CO 2 , respectively, in the ambient air (μg cm −3 ) c i , c′ i concentration of water vapour and CO 2 , respectively, in the intercellular spaces (μg cm −3 ) Tr transpiration (μg cm −2 s −1 ) f n net photosynthesis (μg cm −2 s −1 )

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