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OPIOID-PEPTIDES AND RECEPTORS - LOCALIZATION, INTERACTIONS AND RELATIONSHIPS TO OTHER MOLECULES IN THE RODENT BRAIN, ESPECIALLY THE HIPPOCAMPAL-fORMATION

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PROGRESS IN HISTOCHEMISTRY AND CYTOCHEMISTRY 1989年第3期20卷 1-&页

作者： STENGAARDPEDERSEN, K AARHUS UNIV INST MED BIOCHEMHISTOCHEM UNITDK-8000 AARHUS CDENMARK UNIV COPENHAGEN INST PATHOLHISTOCHEM UNITDK-1168 COPENHAGEN KDENMARK

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MODIfICATIONS Of THE RELATIVE PROTEOLIPID COMPOSITION IN THE ATP SYNTHASE Of A RESPIRATORY COMPETENT MUTANT Of SACCHAROMYCES-CEREVISIAE

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BIOCHIMICA ET BIOPHYSICA ACTA 1989年第2期985卷 127-132页

作者： MANON, S GUERIN, M CNRS INST BIOCHIM CELLULAIRE & NEUROCHIM 1 RUE CAMILLE ST SAENS F-33077 BORDEAUX FRANCE

A comparative study of the proteolipid composition of the f0-sector of the ATP synthase of wild-type strain of Saccharomyces cerevisiae and of nuclear mutants, modified at the level of the oxidative phosphorylation due to an enhanced proton permeability of the inner membrane, was carried out. Analysis of the crude proteolipid extract by electrophoresis and high liquid performance chromatography showed some differences at the level of mitochondrial DNA encoded proteolipids. Subunit 6 and in particular subunit 8 were present in reduced amounts, whereas subunit 9 was present in equal amounts in both types of strain. However, the phosphate binding affinity of subunit 8 was the same in wild-type and mutant strains. The fact that subunit 6 and subunit 8 are cotranscripted on a single mRNA led to the problem of the regulation of the mitochondrial synthesis of these two proteins by a nuclear gene.

关键词： Proteolipid composition Respiratory mutant ATPase, subunit 8 ( S. cerevisiae mitochondria) BSA bovine serum albumin DCCD dicyclohexyl-carbodiimide CCCP p -carbonylcyanide m -chlorophenylhydrazone. Enzyme ATP synthase ATP phosphohydrolase EC 3.6.1.34.f 0 f 1 integral membrane and peripheral portions of ATP synthase

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A RAPID METHOD fOR QUANTITATION Of ANTIVIRAL ANTIBODIES

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JOURNAL Of IMMUNOLOGICAL METHODS 1989年第1期119卷 83-94页

作者： PETERSON, JD KIM, JY MELVOLD, RW MILLER, SD WALTENBAUGH, C NORTHWESTERN UNIV SCH MED DEPT MICROBIOL IMMUNOL 6-693 MORTON BLDG 303 E CHICAGO AVE CHICAGO IL 60611 USA NORTHWESTERN UNIV SCH MED GRAD PROGRAM NEUROSCI CHICAGO IL 60611 USA

This paper examines the parameters necessary for the efficient measurement of anti-Theiler''s murine encephalomyelitis virus (TMEV) antibodies in an affinity-dependent manner using a variation of a solid-phase particle concentration fluorescence immunoassay (PCfIA). By allowing antibody to react with fluorochrome-labelled virus in fluid phase and subsequently capturing the resulting virus-antibody complexes with anti-immunoglobulin coated polystyrene particles (fluid-phase PCfIA), the present assay allows for both greater sensitivity, specificity and preservation of conformational viral epitopes than do solid-phase immunoassays. fluid-phase PCfIA PCfIA proved to be a more rapid quantitative assay than ELISA and significantly diminished non-specific binding by both untreated and heat-inactivated normal mouse sera. This methodology also allowed us to perform competition assays and to determine the dissociation kinetics of anti-viral antibody preparations, investigations which cannot generally be performed as solid-phase immunoassays. Thus fluid-phase PCfIA is a rapid and efficient immunoassay with excellent reproducibility and great versatility.

关键词： fluorescence immunoassay Viral antibody Particle concentration fluorescence immunoassay Theiler's murine encephalomyelitis virus CV coefficient of variation ELISA enzyme-linked immunosorbent assay fIA fluorescence immunoassay fITC fluorescein isothiocyanate f / P fluorochrome: protein ratio GAMI goat anti-mouse immunoglobulin IBS isotonic buffered saline IBST-BSA isotonic buffered saline, Tween 20, bovine serum albumin containing buffer MEf1 type 2 poliovirus OPD o -phenylenediamine PCfIA particle concentration fluorescence immunoassay PEG polyethylene glycol RfU relative fluorescence unit RIA radio-immunoassay TMEV Theiler's murine encephalomyelitis virus TMEV-fITC Theiler's murine encephalomyelitis virus conjugated with fluorescein isothiocyanate TNE Tris sodium chloride EDTA buffer TxRd Texas Red sulforhodamine 101 monosulfonyl chloride

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THE PRESENT LACK Of EVIDENCE fOR UNIQUE RODENT GERM-CELL MUTAGENS

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MUTATION RESEARCH 1989年第1期212卷 55-66页

作者： ADLER, ID ASHBY, J ICI PLC CENT TOXICOL LAB MACCLESFIELD SK10 4TG CHESHIRE ENGLAND

Six chemicals, diethylhexyl phthalate (DEHP), ethanol, cyclohexylamine (CHA), sodium saccharin (NaS), cadmium chloride (CdCl2) and triflupromazine (TfP), were suggested to be unique germ-cell mutagens (Auletta and Ashby, 1988) by the GeneTox Workgroups of the U.S. Environmental Protection Agency (EPA). If this is a correct classification it would have major consequences when screening for mutagenicity and when labelling genotoxic substances. However, our re-evaluation of the GeneTox literature, including some more recent publications, has failed to find substantive evidence that any of these chemicals have been unequivocally established as having unique mutagenic activity in germ cells. for DEHP, NaS and TfP the evidence for genotoxic/mutagenic effects is questionable, in both germinal and somatic cells. Ethanol and CdCl2 showed clastogenic activity, but it was not restricted to germ cells. Both, ethanol and cadmium salts, appear to induce aneuploidy. The unconfirmed clastogenic effect of CHA was restricted to rats, but it occurred in both bone marrow and spermatogonia. Therefore, the general observation that rodent germ-cell mutagens are also genotoxic in somatic cells in vivo (Brusick, 1980; Holden, 1982) remains valid.

关键词： Chemical mutagens Genotoxicity Germ cells Somatic mutations AT ascites tumour cells BLA blastocysts BL-MN blood micronucleus test BM bone marrow b.o. before ovulation C-Mit colchicine-like mitoses CA chromosomal aberrations CdCl 2 cadmium chloride CHA cyclohexylamine Chin H Chinese hamsters DEHP diethylhexyl phthalate DLT dominant lethal test d.w. drinking water f females fS fertility study Golden H golden hamsters HCG human chorionic gonadotropin HMA host-mediated assay HPL human peripheral lymphocytes HTT herritable translocation test i.m. intramuscular injection i.p. intraperitoneal injection l.d. liquid diet LD late fetal death m males MI first meiotic metaphasel MII MI second meiotic metaphase MNT micronucleus test MST mouse spot test NA numerical aberrations NaS sodium saccharin p.c. post conception PN pronuclear stages p.o. gavage RT reciprocal translocations s.c. subcutaneous injection SCE sister-chromatid exchanges SPA sperm-head abnormalities SG spermatogonial stem cells TfP triflupromazine

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ULTRASTRUCTURAL-STUDY Of TROGLODYTELLA-GORILLAE, A CILIATE fROM THE INTESTINE Of THE GORILLA - SYSTEMATICS Of ENTODINIOMORPHID CILIATES

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EUROPEAN JOURNAL Of PROTISTOLOGY 1989年第3期24卷 225-237页

作者： BONHOMME, A GRAIN, J COLLET, JY ZOOL UER SCI REINS FRANCE CTR INT RECH MED FRANCEVILLE GABON

This ultrastructural study of the entodiniomorphid Troglodytella gorillae provides information on the constitution of the cortex, which is composed of the plasma membrane, a thick epiplasm, microtubules, and a microfibrallar ecto-endoplasmic limit (cortex type I). This observation allows us to group the family Troglodytellidae with the families Cycloposthiidae and Ophryoscolecidae. These 3 families should be separated from the families Tripalmariidae and Spirodiniidae which possess a more complex cortex, with longitudinal dense tracts inserted between the microtubular layer and the microfibrillar ecto-endoplasmic limit (type II). Terminology is discussed.

关键词： A amidon Am amylopectine AV avant de la cinétie Ax axosome B bouchon dense Ba bactéries Cl-4 bandes ciliées Ca caryophore Ci cil Cil ciliophore Cp cytoprocte Cph cytopharynx Cst cytostome D côté droit des cinéties E épiplasme En endoplasme f couche fibreuse externe de la nasse pharyngienne fa fibres axiales fp fibres périphériques G côté gauche des cinéties Ga granule axial Hy hydrogénosome I inclusion dense Kd fibre cinétodesmale Ki cinétie Ks cinétosome LEE limite ecto-endoplasmique LM liaisons longitudinales moyennes LP liaisons longitudinales profondes M membrane cellulaire Ma macronoyau Mc membrane cellulaire Mi micronoyau Mt microtubules Nd némadesme Np nasse pharyngienne Ps plaques squelettiques R rideaux de microtubules S syncils Se septum T fibres transverses Tr travées de matériel diffus V1–4 vacuoles digestives Vb ouverture vestibulaire Ve vésicules ergastoplasmiques Troglodytella Ciliés Entodiniomorphes Systématique Cortex

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THE MULTIELEMENTAL ANALYSIS Of BONE - A REVIEW

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BIOLOGICAL TRACE ELEMENT RESEARCH 1989年第3期19卷 195-232页

作者： ZWANZIGER, H 1. Section of Chemistry Karl Marx University Leipzig German Democratic Republic

Numerous elements, especially metals, possess biological activity. But until now, they were of interest mainly as ingredients of foods.

关键词： Bones metals in elements essential and nonessential Co Cu fe Ni Pb f Mg Na

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CORRELATIONS BETWEEN LEVELS Of MITE AND CAT ALLERGENS IN SETTLED AND AIRBORNE DUST

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JOURNAL Of ALLERGY AND CLINICAL IMMUNOLOGY 1989年第4期83卷 776-783页

作者： SWANSON, MC CAMPBELL, AR KLAUCK, MJ REED, CE MAYO CLIN & MAYO FDN ALLERG DIS & INTERNAL MED ROCHESTER MN 55905 USA

Thirty homes in Rochester, Minn., 20 of which housed cats, were studied to compare cat and mite allergen concentrations in airborne and settled dust. With Air Aentinels in the bedroom and living room for airborne collections, and a Sample Vac for collections from living room carpet and bedroom mattress, immunochemical quantifications of each were made with various radiometric assays with polyclonal and monoclonal antibodies. The most significant correlations were found between airborne mite in the bedroom and living room (p < 0.001) and airborne mite in the bedroom and dust from the bedroom mattress (p < 0.001). Most houses had specific epitopes of both Dermatophagoides pteronyssinus and D. farinae, but D. farinae was present in greater amounts. In seven houses we found > 10 .mu.g of group I mite allergen per gram of settled dust, indicating that some houses in Minesota have concentrations of mite allergens hich enough to cause allergic disease, even in the winter. Clinical interpretation of these data on air levels in hampered by uncertainty as to whether symptoms are more closely related to average steady-state exposure, which we measured, or to brief heavy concentrations. further epidemiologic studies are needed to define the concentration of mite and cat allergens in the air that are risk factors for allergic disease. The concentration of cat allergen correlated with mite allergen in the air, but not in settled dust, presumably reflecting the fact that both are influenced by ventilation rate.

关键词： Der p I Group I allergen from Dermatophagoides pteronyssinus (Dp) Der f I Group I allergen from D. farinae (Df) Der p I equivalents Allergens in whole mite extract containing stated amount of Der p I measured by an antibody that cross-reacts with Der f I fel d I Major allergen from cat felis domesticus MAb Monoclonal antibody

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THE ABSOLUTE SIZE Of A PHOTOSYNTHETIC UNIT

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BIOCHIMICA ET BIOPHYSICA ACTA 1989年第2期974卷 119-140页

作者： MAUZERALL, D GREENBAUM, NL The Rockefeller University New York NY (U.S.A.)

The photosynthetic unit is a complex of pigments and proteins coupled to a reaction center where the initial light-driven charge separation of photosynthetic reactions takes place. The ‘size’ of the photosynthetic unit is defined as the number of light-absorbing pigment molecules contributing excitation energy to a reaction center. It is an important parameter in the study of the structure and function of photosynthetic membranes. Several approaches used for relative and absolute measures of photosystem stoichiometry and size are evaluated, and results achieved by these methods are compared. The concept of the absolute size of a photosynthetic unit leads to a simple relation between the size of the oxygen forming unit, the total chlorophyll per O 2 (the classical Emerson-Arnold unit), and the quantum requirement for O 2 formation. These measurements lead to further understanding of the mechanisms of energy transfer and of the adaptive mechanisms of plants to varied environments.

关键词： Optical cross section Poisson distribution Trap Reaction center Single turnover flash Quantum requirement Energy transfer fluorescence induction Antenna, light-harvesting Pigment protein RC reaction center PSU photosynthetic unit PSU( z ) photosynthetic unit defined by product ( z ) RC I (II) reaction center of Photosystem I (II) PS I (II) Photosystem I (II) N I ( N II) number of pigment molecules in PS I (PS II) n I ( n II) number of RC I (RC II) Chl chlorophyll σ( z ) optical cross section of PSU ( z ) measured via component z σ(pig) optical cross section of pigment in vivo Q A quinone with maximum absorption at 325 nm, primary electron acceptor of reaction center II P-700 chlorophyll with maximum absorption at 700 nm, primary electron donor of reaction center I Y ( z ) yield of product z per hit of an RC f 0 initial fluorescence (fluorescence induction curve) f v variable fluorescence (fluorescence induction curve) f max maximal fluorescence (fluorescence induction curve) LHC II (I) light-harvesting complex of Photosystem II (I) DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea DCIP 2,6-dichloroindophenol ATP adenosine 5′-triphosphate Δε difference extinction coefficient

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CHARACTERIZATION Of AN INHIBITOR Of PROTEIN-SYNTHESIS INITIATION fROM MOUSE ERYTHROLEUKEMIA-CELLS

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BIOCHIMICA ET BIOPHYSICA ACTA 1989年第1期1009卷 61-69页

作者： BADER, M SARRE, Tf INST BIOL 3 SCHANZLESTR 1 D-7800 FREIBURG GERMANY

This study describes the partial purification of a translational inhibitor from mouse erythroleukemia (MEL) cells. It is present in MEL cells induced to erythroid differentiation and in uninduced cells in approximately equal amounts. The inhibitor blocks initiation but not elongation of in vitro protein synthesis in the rabbit reticulocyte lysate and in extracts prepared from induced or uninduced MEL cells. Nuclease-resistance, heat-sensitivity and the chromatographic behaviour of the inhibitor indicate that it is a protein with a relative molecular mass of approx. (45-70) .cntdot. 103. The inhibitor has no ***. phosphorylating activity and does not affect the formation of the ternary complex [eIf-2 .cntdot. GTP .cntdot. Met-tRNAf] nor the binding of Met-tRNAf to the 40 ribosomal subunit. The inhibitor interferes with the binding of mRNA to the 43 S preinitiation complex, independent of the presence of the m7GTP cap of the mRNA.

关键词： Protein synthesis In vitro translation mRNA binding Inhibition (Mouse erythroleukemia cell) MEL mouse erythroleukemia eIf eukaryotic protein synthesis initiation factor CTAB cetyltrimethylammonium bromide GDPCP guanyl(β,γ-methylene)diphosphonate TCA trichloroacetic acid SDS sodium dodecylsulfate PAGE polyacrylamide gel electrophoresis Met-tRNA f eukaryotic initiator methionyl-tRNA a.u. arbitrary unit

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IGG ANTIBODY AGAINST fORMALDEHYDE HUMAN-SERUM PROTEINS - A COMPARISON WITH OTHER IGG ANTIBODIES AGAINST INHALANT PROTEINS AND REACTIVE CHEMICALS

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JOURNAL Of ALLERGY AND CLINICAL IMMUNOLOGY 1989年第3期84卷 359-366页

作者： PATTERSON, R DYKEWICZ, MS EVANS, R GRAMMER, LC GREENBERGER, PA HARRIS, KE LAWRENCE, ID PRUZANSKY, JJ ROBERTS, M SHAUGHNESSY, MA ZEISS, CR NORTHWESTERN UNIV SCH MED DEPT MED ALLERGY IMMUNOL SECT 303 E CHICAGO AVE CHICAGO IL 60611 USA

Immune responses to formaldehyde (f) have been recorded for seven decades. More recently, sensitive assays for antibody against f-human serum albumin (HSA) have been reported. IgG antibody against f-HSA has been said to correlate with symptoms against f-HSA. We report on 61 serum samples analyzed for IgG antibodies against f-HSA. IgG antibodies against f-HSA were most prevalent in subjects who had received intravenous f. In no case (either presumed symptomatic to f or with IgG antibody against f-HSA) was there a correlation of serologic results with symptoms. We also reviewed inhalation disease caused by chemicals and proteins acting as immunogens and report that at this time there is no evidence that gaseous f meets the criteria for causation of inhalational IgG-mediated lung disease by clinical or serologic studies. Very high IgG antibody levels occur in respiratory immunologic inhalational disease, and the absence of these high IgG levels against f is strong evidence against f or f proteins being an inhalational antigen of significance.

关键词： f formaldehyde HSA Human serum albumin MDI Diphenylmethane diisocyanate TMA Trimellitic anhydride

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