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STUDIES Of THE METATHORAX Of THE TROUT-STREAM BEETLE, AMPHIZOA-LECONTEI MATTHEWS (COLEOPTERA, AMPHIZOIDAE) - CONTRIBUTION TOWARDS CLARIfICATION Of THE SYSTEMATIC POSITION Of AMPHIZOIDAE

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INTERNATIONAL JOURNAL Of INSECT MORPHOLOGY & EMBRYOLOGY 1988年第1期17卷 63-81页

作者： BEUTEL, RG UNIV MANITOBA DEPT ENTOMOL WINNIPEG R3T 2N2 MANITOBA CANADA

Skeleton and musculature of the metathorax and the abdominal nerve cord of Amphizoa lecontei (Coleoptera:Amphizoidae) Matthews were examined and compared to those of other members of Adephaga (Coleoptera). The following characters may be important for phylogenetic analysis. The narrow prescutal area and moderately developed mesophragma with distinctly separated median phragmal lobes in Amphizoa is considered as plesiomorphic, whereas the median phragmal lobes lysing closely together or fused is a possible synapomorphy of Hygrobiidae and Dytiscidae. The short 1st axillary sclerite of Amphizoa may be an autapomorphy of the group. The weakly arched notum and the poorly developed metaphragma are obviously the result of tendency towards flightlessness. The anepisternum meets the middle coxal cavity. The posternal process does not reach the meta "sternum" ("sternum" = preepisternum + basisternum presternum + spinasternum 2). The specialized pro-meso-meta "sternal" articulation in Hygrobia and Dytiscidae is considered a synapomorphy of both groups. The amphizoid metafurca is distinctly reduced in size. Pouch-like anterolateral excavations are considered plesiomorphic. Strong dorsolateral and weak ventrolateral projections of the metafurca may be a synapomorphy of Hygrobiidae and Dytiscidae. The hind legs of Amphizoa are not adapted for swimming, except for very sparse fringes of thin setae. Swimming hind legs are considered as a synapomorphy of Hygrobiidae and Dytiscidae. The main flight muscles were degenerated in all specimens of Amphizoa examined. Absence of muscle (M) 65 may be autapomorphic for Amphizoidae. Presence of M 72 is a symplesiomorphy of Amphizoidae and Dytiscidae. M 81 and M 83 are absent. The strong M 82 is considered as plesiomorphic. The moderate size of M 85 is connected with the lack of swimming ability. The elongate abdominal nerve cord in Amphizoa points towards a sistergroup relationship between Hygrobiidae and Dytiscidae in both of which the abdomi

关键词： Skeleton musculature abdominal nerve cord Hydradephaga phylogeny Aes anepisternum Alc alacristae anp anterior notal process Apl anapleural suture Ax axillary sclerite (l, 2, 3) Bas basalare dSc dorsal sclerite of the epimeron Dshs dome shaped sclerite f metafurca fe metafemur G3 metathoracic ganglion + 1st abdominal ganglion G I, II subsequent abdominal ganglia Ics intercoxal septum Kes katepisternum Mbas muscle disc of the basalare Mc metacoxa mls median longitudinal suture mml median mesophragmal lobe Mstp metasternal process Pes preepisternum Plr pleural ridge Pls pleural suture pmnp postmedian notal process Pn postnotum pnp posterior notal process Prs prealar sclerite pwp pleural wing process Sa subalare Sc scutum Scl scutellum Sma semimembranous area of the epimeron Stg I first abdominal stigma Tr trochanter tr transverse ridge vSc ventral sclerite of the epimeron

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DIETARY INHIBITORS Of MUTAGENESIS AND CARCINOGENESIS

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MUTATION RESEARCH 1988年第2期202卷 429-446页

作者： HAYATSU, H ARIMOTO, S NEGISHI, T Faculty of Pharmaceutical Sciences Okayama University Japan.

Dietary inhibitors of mutagenesis and carcinogenesis are of particular interest because they may be useful for human cancer prevention. Several mutagenesis inhibitors have been demonstrated to be carcinogenesis inhibitors also, e.g., ellagic acid, palmitoleic acid, and N-acetylcysteine. This means that the search for mutagenesis inhibitors may be useful for discovering anticarcinogenic agents. Many mutagenesis inhibitors have been discovered by the use of short-term assays, particularly the Ames Salmonella test. This simple in vitro system has provided opportunities to elucidate the mechanisms of inhibition. The elucidation of the mechanism may allow us to infer the possible anticarcinogenic activity of the reagent. In this chapter, inhibitors of mutagenesis and carcinogenesis that can arise as components of diet have been reviewed. Most of the inhibitors have been demonstrated to be effective against a specific class of mutagens or carcinogens. Therefore, it may be argued that these inhibitors are antagonistic only to those particular agents. Here again, understanding of the mechanisms of these inhibitions is necessary for the assessment. Dietary inhibitors reviewed in this article include: (1) as inhibitors of mutagenesis: porphyllins, fatty acids, vitamins, polyphenols, and sulfhydryl compounds, (2) as inhibitors of carcinogenesis: vitamins A, E and C, ellagic acid, sulfhydryl compounds, fats, selenium, calcium, and fiber. further studies in this area of science appear to help establish the recipe of a healthy diet.

关键词： Dietary inhibitors Vitamins Polyphenols Porphyllins Sulfhydryl compounds fiber Trp-P-1 3-amino1,4-dimethyl-5 H -pyrido[4,3- b ]indole Trp-P-2 3-amino-1-methyl-5 H -pyrido[4,3- b ]indole Trp-P-2(NHOH) 3-hydroxyamino-1-methyl-5 H pyrido[4,3- b ]indole Glu-P-1 2-amino-6-methyldipyridol[1,2- a ,3′,2′- d ]imidazole Glu-P-2 2-aminodipyridol[1,2- a ,3′2′- d ]imidazole Glu-P-1(NHOH) 2-hydroxyamino-6-methyldipyrido[1,2- a ,3′,2′- d ]imidazole IQ 2-amino-3-methylimidazo[4,5- f ]quinoline MeIQ 2-amino-3,4-dimethyllimidazo[4,5- f ]-quinoline MeIQx 2-amino-3,8-dimethyllimidazo[4,5- f ]-quinoxaline B(a)P benzo[ a ]pyrene B(a)P diol epoxide 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene DBMA 7,12-dimethylbenz[ a ]anthracene NDMA N -nitrosodimethylamine MNU N -methylnitrosourea MNNG N -methyl- N ′-nitro- N -nitrosoguanidine DMH 1,2-dimethyl-hydrazine Af-2 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide

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EffECT Of CONTINUOUS SHADING ON GROWTH, fLOWERING AND NUTRIENT-UPTAKE Of PASSIONfRUIT

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SCIENTIA HORTICULTURAE 1988年第1-2期35卷 77-88页

作者： MENZEL, CM SIMPSON, DR Maroochy Horticultural Research Station Queensland Department of Primary Industries P.O. Box 5083 Sunshine Coast Mail Centre Nambour Qld. 4560 Australia

Low irradiance (mean total shortwave radiation of 2.1 or 6.3 MJ m-2 day-1 compared to 10.5, 14.6 or 20.9 MJ m-2 day-1) increased shoot elongation and reduced leaf area, plant dry weight, and floral bud and open flower number in the Passiflora edulis f. edulis .times. P. edulis f. flavicarpa hybrid, E-23. Shading also increased the proportion of stem contributing to plant dry matter and reduced the proportion of root, and altered a range of plant morphological characteristics. The decline in shootweight at low irradiance was associated with a fall in the total shoot content of most nutrients. The shoot tissue levels of Ca, Mg and fe rose and those of P fell at lower irradiance. The levels of S and Zn rose as irradiance fell to 14.6 or 10.5 MJ m-2 day-1. The concentration of the other nutrients (N, K, Na, Mn, Cu, B and Al) was not consistently affected by shading. It was concluded that irradiance may be one of the major environmental factors contributing to seasonal fluctuations in passionfruit flowering and fruit set. Potential production was reduced by each reduction in irradiance below full sun. The effect of irradiance on vine development did not appear to involve changes in nutrient uptake.

关键词： flowering growth irradiance nutrient uptake Passiflora edulis f edulis × P. edulis f flavicarpa passionfruit

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THE GAMMA-SUBUNIT Of ATP SYNTHASE fROM SPINACH-CHLOROPLASTS - PRIMARY STRUCTURE DEDUCED fROM THE CLONED CDNA SEQUENCE

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fEBS LETTERS 1988年第1期232卷 221-226页

作者： MIKI, J MAEDA, M MUKOHATA, Y fUTAI, M OSAKA UNIV FAC SCIDEPT BIOLTOYONAKAOSAKA 560JAPAN

cDNA clones encoding the gamma-subunit of chloroplast ATP synthase were isolated from a spinach library using synthetic oligonucleotide probes. The predicted amino acid sequence indicated that the mature chloroplast gamma-subunit consists of 323 amino acid residues and is highly homologous (55% identical residues) with the sequence of the cyanobacterial subunit. The positions of the four cysteine residues were identified. The carboxyl-terminal region of the chloroplast gamma-subunit is highly homologous with those of the gamma-subunits from six other sources (bacteria and mitochondria) sequenced thus far.

关键词： H + -ATPase f 1 γ-Subunit Cyanobacteria cDNA (Spinach chloroplast)

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IDENTIfICATION Of NUCLEUS-ENCODED fOI PROTEIN Of BOVINE HEART MITOCHONDRIAL H+-ATPASE AS A fUNCTIONAL PART Of THE fO MOIETY

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fEBS LETTERS 1988年第1-2期237卷 9-14页

作者： ZANOTTI, f GUERRIERI, f CAPOZZA, G HOUSTEK, J RONCHI, S PAPA, S UNIV MILAN INST PHYSIOL VET & BIOCHEMI-20122 MILANITALY

The f 0 I protein of apparent M r 27000, previously characterized [(1988) Eur. J. Biochem. 173, 1–8] as a genuine component of bovine heart f 0 , has been sequenced and shown to be identical with the nucleus encoded 24668 Da protein characterized earlier [(1987) J. Mol. Biol. 197, 89–100]. It is directly shown by proteolytic cleavage and reconstitution experiments that this protein, denoted here as PVP from the single-letter codes of the last three residues of the N-terminus, is involved in proton conduction by f 0 and in its sensitivity to oligomycin.

关键词： H + -ATPase f 0 complex Oligomycin H + conduction f 1 , catalytic part of mitochondrial H + -ATPase f 0 , membranous sector of mitochondrial H + -ATPase f 6 , coupling factor 6 involved in the binding of f 1 to f 0 OSCP, oligomycin-sensitivity-conferring protein ESMP, submitochondrial particles prepared in the presence of EDTA USMP, submitochondrial particles devoid of f 1 (see section 2) PAGE, polyacrylamide gel electrophoresis CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate

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INTERACTIONS BETWEEN A PARAMAGNETIC ANALOG Of CHOLESTEROL AND fILIPIN

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BIOCHIMICA ET BIOPHYSICA ACTA 1988年第1期939卷 102-110页

作者： MAURIN, L BANCEL, f MORIN, P BIENVENUE, A UNIV MONTPELLIER 2 BIOL PHYS CHIM LAB CNRS UA 530 F-34060 MONTPELLIER FRANCE

A paramagnetic analogue of cholesterol (called 25-doxyl-27-norcholesterol (CNO)), labeled near the w-end of the hydrophobic tail, was used to study interactions of cholesterol with filipin. We observed by electron microscopy that CNO- and cholesterol-filipin complexes are structurally equivalent. Two kinds of complexes were seen by ESR spectroscopy and electron microscopy, depending on the stoichiometric R ratio between the antibiotic and sterol. When R was high, an immobilized ESR spectrum appeared, showing strong imbrication between CNO and filipin. When R was nearer to unity, an exchange-broadened spectrum emerged, corresponding to a new phase that was very rich in CNO (a fast exchange between spins could occur by nearest contacts). CNO was easily displaced from its complex (i) by gradual addition of genuine cholesterol;and (ii) by an excess of phospholipids, owing to the very poor affinity of CNO (and cholesterol, by extension) for filipin in the lipidic phase. Almost no difference appeared between the ESR spectra of oriented samples, i.e. the probe showed no long-range order in any complex of CNO with filipin.

关键词： Cholesterol analog filipin ESR Electron microscopy CNO 25-doxyl-27-norcholesterol fl filipin PC egg yolk phosphatidylcholine I immobilized spectrum component E exchange-broadened component f fast mobile component

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ALLERGY IN LONG-TERM HEMODIALYSIS .2. ALLERGIC AND ATOPIC PATTERNS Of A POPULATION Of PATIENTS UNDERGOING LONG-TERM HEMODIALYSIS

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JOURNAL Of ALLERGY AND CLINICAL IMMUNOLOGY 1988年第3期81卷 590-597页

作者： BOUSQUET, J MAURICE, f RIVORY, JP SKASSABROCIEK, W fLORENCE, P CHOUZENOUX, R MION, C MICHEL, fB CTR HEMODIALYSE LANGUEDOC MEDITERRANEEN MONTPELLIER FRANCE CLIN NEPHROL MONTPELLIER FRANCE

Maintenance hemodialysis is widely used throughout the world, and anaphylactic reactions appear to be increasing in number and severity. However, the exact incidence of sensitization and the role of atopy in these reactions are not yet fully understood. All of the 111 patients routinely dialyzed in a center were tested. All patients had a complete investigation of atopy, RAST to chemicals released during the procedure of dialysis (ethylene oxide (Eto), formaldehyde, phtalic anhydride, and toluene diisocyanate), skin tests with the effluent, and the titration of blood eosinophils. The incidence of atopy was found to be lower (13.5%) than in the normal population of the area. Skin tests with either histamine or allergens are significantly (p < 0.001) smaller than those of nondialyzed subjects, and this method does not appear to be ideal in this population of patients. Eto sensitivity ranked first (5.5%), followed by phthalic anhydride sensitivity (3.6%);5/6 patients who had a sensitivity to Eto and/ or phthalic anhydride present symptoms during dialysis, but they never were life threatening. formaldehyde RAST was only found in one patient who had a life-threatening reaction. finally, three patients presenting pruritus had positive skin prick tests with the effluent of the dialyzer. All patients having a first use syndrome and 80/81 symptom-free patients did not have serum specific IgE against the released chemicals, 5/17 patients who had a pruritus during dialysis had either positive RAST to released chemicals or skin tests to the effluent, 5/8 patients who suffered from anaphylaxis had positive RAST to released chemicals, but only those who had a positive RAST presented a severe reaction. Atopy appeared to predispose to the development of allergy to released chemicals, since 40% of atopic individuals were sensitized to them. Total serum IgE was significantly (p < 0.001) increased in allergic subjects. Blood eosinophils were significantly (p < 0.01) increased in aller

关键词： f PA TDI

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A SIMPLE METHOD fOR ASSESSMENT Of RAT CYTOCHROME-P-448 ISOZYMES RESPONSIBLE fOR THE MUTAGENIC ACTIVATION Of CARCINOGENIC CHEMICALS

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MUTATION RESEARCH 1988年第5期203卷 333-338页

作者： DEGAWA, M UENO, H MIURA, S OHTA, A NAMIKI, M Department of Hygienic Chemistry Tohoku University Sendai Japan.

Cytochrome P-448H/L-enriched and cytochrome P-448L-enriched microsomes were prepared from the livers of Sprague-Dawley rats treated with 3-methylcholanthrene (MC) and with a combination of MC and carbon tetrachloride, respectively, and their activities for mediating mutagenic activation of 9 carcinogenic aromatic amines and benzo[a]pyrene, which are found to be different from cyt. P-450 isozymes as to mutagenic activation, were compared on the basis of microsomal cytochrome P-450 content using Salmonella typhimurium TA98 as a tester bacterium. With regard to the substrate-specificity of cytochrome P-448 isozymes, the present results reflected the reported results with use of a cytochrome P-450-reconstituted system. These findings indicate that the mutation test with cytochrome P-448H/L-enriched and cytochrome P-448L-enriched microsomes could be used as a simple method for the determination of the cytochrome P-448 isozymes responsible for the mutagenic activation of carcinogens and mutagens without the use of a cytochrome P-450-reconstituted system.

关键词： Cytochrome P-448 isozymes, rat 3-Methylcholanthrene cyt cytochrome(s) MC 3-methylcholanthrene CCl 4 carbon tetrachloride B(a)P benzo[ a ]pyrene Trp P-1 3-amino-1,4-dimethyl-5 H -pyrido[4,3- b ]indole acetate Trp P-2 3-amino-1-methyl-5 H -pyrido[4,3- b ]indole acetate Glu P-1 2-amino-6-methyldipyrido[1,2- a :3′,2′- d ]imidazole hydrochloride Glu P-2 2-amino-dipyrido[1,2- a :3′,2′- d ]imidazole hydrochloride AαC 2-amino-9 H -pyrido[2,3- b ]indole acetate MeAαC 2-amino-3-methyl-9 H -pyrido[2,3- b ]indole acetate IQ 2-amino-3-methylimidazo[4,3- f ]quinoline MeIQ 2-amino-3,4-dimethylimidazo[4,5- f ]quinoline SKf 525A 2-diethylaminoethyl-2,2-diphenylvalerate hydrochloride Pb phenobarbital sodium salt 3-MeO-AAB 3-methoxy-4-aminoazobenzene MoAb monoclonal antibody ELISA protein A-enzyme-linked immunosorbent assay

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IMMUNOLOGICAL SUBTYPES Of ACUTE LYMPHOBLASTIC-LEUKEMIA IN NORTH INDIA

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LEUKEMIA RESEARCH 1988年第8期12卷 673-678页

作者： BHARGAVA, M KUMAR, R KARAK, A KOCHUPILLAI, V ARYA, LS MOHANAKUMAR, T ALL INDIA INST MED SCI DEPT MICROBIOL NEW DELHI 110029 INDIA ALL INDIA INST MED SCI DEPT MED ONCOL NEW DELHI 110029 INDIA ALL INDIA INST MED SCI DEPT PEDIAT NEW DELHI 110029 INDIA VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA DEPT SURG RICHMOND VA 23298 USA

Pretreatment immunologic marker analysis in 152 adult and childhood patients of ALL and ALL/lymphoma employing multiple monoclonal antibodies and hetero-antisera revealed three major subgroups, i.e. T-ALL (37.7%), N-ALL (33.1%) and C-ALL (21.5%). The early age peak was absent, males predominated in all the subgroups and T-ALL had increased incidence of thymic mass. Leucocyte counts of 50,000 .times. 106/l were equally frequent in the three groups. T-ALL showed marked heterogeneity by showing a variety of markers such as T-helper/inducer, T-suppressor/cytotoxic, p-24, Ia and CALLA. These results show a high prevalance of unfavorable prognostic factors in ALL in our geographic region which might be related to socioeconomic and/or environmental factors.

关键词： ALL C-ALL T-ALL immunophenotype leukemia socioeconomic status ALL acute lymphoblastic leukemia SBB Sudan black B PAS periodic acid Schiff NSE alpha naphthyl acetate esterase AP acid phosphatase MoAbs monoclonal antibodies SIg surface immunoglobulin CIg cytoplasmic immunoglobulin Tdt terminal deoxynucleotidyl transferase ER spontaneous rosetting with sheep red blood cells CALLA common ALL antigen T-ALL T-cell acute lymphoblastic leukemia C-ALL common acute lymphoblastic leukemia N-ALL null cell acute lymphoblastic leukemia B-ALL B-cell ALL AUL acute unclassified leukemia fAB french-American-British M male f female

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AN ELECTROCHEMICAL STUDY Of ENERGY-DEPENDENT POTASSIUM ACCUMULATION IN ESCHERICHIA-COLI .12. K+-DEPENDENT ATPASE ACTIVITY (ARGUMENTS fOR THE STRUCTURAL ASSOCIATION Of H+-ATPASE WITH K+-IONOPHORE)

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BIOELECTROCHEMISTRY AND BIOENERGETICS 1988年第3期19卷 353-357页

作者： MARTIROSOV, SM OGANDJANIAN, ES TRCHOUNIAN, AA Department of Biophysics Biological Faculty of Yerevan State University 375049 Yerevan U.S.S.R.

K+-dependent and DCC-sensitive ATPase activity is observed only in anaerobically grown E. coli and only under the structural integrity of both the H+-ATPase (ECf0f1) and K+-ionophore (the Trk system). The results confirm the data of the H+-K+-exchange analyses and indicate that the ECf0f1 and Trk systems in anaerobically grown bacteria work as a united supercomplex inside of which the direct energy transfer occurs without mediation by .DELTA..hivin..mu.H+.

关键词： EC refers to proteins from E. coli cells f 0 f 1 is H + -ATPase complex f 1 H + -ATPase, f 0 the proteolipid α K + is the external K + activity Δ μ H + the difference of the electrochemical potentials for H + f is faraday's constant U m the membrane potential DCC is N , N′ -dicyclohexylcarbodiimide P i inorganic phosphorus.

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