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CONTROL MECHANISMS Of HUMAN BASOPHIL RELEASABILITY

JOURNAL Of ALLERGY AND CLINICAL IMMUNOLOGY 1986年第5期78卷 974-980页

作者： MARONE, G POTO, S GIUGLIANO, R CELESTINO, D BONINI, S UNIV NAPLES SCH MED 2DEPT CHEMI-80138 NAPLESITALY UNIV ROME LA SAPIENZA SCH MEDDEPT MEDI-00185 ROMEITALY UNIV ROME LA SAPIENZA SCH MEDDEPT INFECT & TROP DISI-00185 ROMEITALY

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fOLDING KINETICS Of MAMMALIAN RIBONUCLEASES

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fEBS LETTERS 1986年第1期204卷 135-139页

作者： LANG, K WRBA, A KREBS, H SCHMID, fX BEINTEMA, JJ UNIV REGENSBURG INST BIOPHYS & PHYS BIOCHEMD-8400 REGENSBURGFED REP GER STATE UNIV GRONINGEN BIOCHEM LAB9747 AG GRONINGENNETHERLANDS

The folding kinetics of seven different pancreatic ribonucleases are compared both under native conditions and within the unfolding transition. In general, the folding kinetics of these proteins are similar despite numerous amino acid substitutions. Ribonucleases with 4-6 proline residues show 80% slow-folding species. for three ribonucleases with 7 prolines this number increases to 90%. Porcine ribonuclease with a unique Pro 114-Pro 115 sequence folds significantly slower than other ribonucleases which do not show this sequence.

关键词： Protein folding RNase Stopped-flow kinetics Protein stability Proline isomerization RNase pancreatic ribonuclease (EC 3.1.27.5) N native protein U f , U s fast- and slow-refolding species of unfolded RNases, respectively GdmCl guanidinium chloride τ time constant of a chemical reaction or phase α relative amplitude of a kinetic phase

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MODIfICATION Of ROOM-TEMPERATURE PICOSECOND CHLOROPHYLL fLUORESCENCE KINETICS IN GREEN-ALGAE BY PHOTOSYSTEM-II TRAP CLOSURE

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fEBS LETTERS 1986年第2期198卷 256-262页

作者： MOYA, I BARBET, JC Laboratoire de Photosynthèse du CNRS 91190 Gif-sur- Yvette France

Room-temperature single-photon timing measurements on green algae at low excitation energies indicate at least three kinetic components of chlorophyll fluorescence when the reaction centres of photosystem (PS) II are open ( f o ) with lifetimes of approx. 50 ps (fast), 200 ps (middle) and 450 ps (slow). The absence, in green algae, of a long-lifetime component (1.4 ns) is at variance with previous reports. Closing the reaction centres gave rise to a similar increase in both the middle and slow lifetimes, by a factor of between 4 and 5, to produce lifetimes of approx. 850 ps and 2 ns, respectively, when all traps were closed ( f max ). The yield and lifetime of the fast component were found to be independent of PS II centre closure. The yields of the middle and slow components were both modified by reduction of the primary stable electron acceptor of PS II (Q a ) with the slow-component yield increasing by a greater degree than the middle-component yield. It was also observed that as PS II reaction centres were closed to photochemistry the fluorescence yield and average lifetime increased proportionally. This trend and the similar increases in the middle and slow lifetimes are indicative of a well-connected system of PS II units favouring exciton transfer between PS II centres. We suggest that the fast kinetic component arises mainly from PS I while the middle and slow components arise from different types of fluorescing unit located in the granal lamellae and associated with system of well-connected PS II.

关键词： Photon counting Chlorophyll fluorescence lifetime Photosystem II Photosynthesis Photosystem I Exciton transfer PS, photosystem LHC, light-harvesting Chl a / b -protein complex DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea Q a , primary stable electron acceptor of PS II f max ( f o , maximum (minimum) chlorophyll fluorescence

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IMMUNOMODULATION BY TAMOXIfEN AND PERGOLIDE

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IMMUNOPHARMACOLOGY 1986年第2期12卷 145-153页

作者： NAGY, E BERCZI, I UNIV MANITOBA FAC MED DEPT IMMUNOL 795 MCDERMOT AVE WINNIPEG R3E 0W3 MANITOBA CANADA

Treatment of rats with tamoxifen citrate or pergolide mesylate was as effective in inhibiting antibody formation and contact sensitivity skin reactions as was hypophysectomy. The immunocompetence of tamoxifen citrate- and pergolide mesylate-suppressed animals could be fully restored by additional treatment with prolactin or growth hormone.

关键词： Cell-mediated immunity Humoral immunity Estrogen Prolactin Pergolide Tamoxifen Growth hormone BPRL bovine prolactin BGH bovine growth hormone DNCB dinitrochlorobenzene f fischer GH growth hormone PGL pergolide mesylate PRL prolactin PBS phosphate-buffered saline SRBC sheep red blood cells TX tamoxifen citrate W Wistar-furth

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THE INTERACTION Of ENfLURANE, HALOTHANE AND THE HALOTHANE METABOLITE TRIfLUOROACETIC-ACID WITH THE BINDING Of ACIDIC DRUGS TO HUMAN-SERUM ALBUMIN - AN INVITRO STUDY

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BIOCHEMICAL PHARMACOLOGY 1986年第4期35卷 557-561页

作者： DALE, O UNIV TRONDHEIM FAC MED DEPT PHARMACOL & TOXICOL EIRIK JARLSGT 10 N-7000 TRONDHEIM NORWAY

The interaction of the volatile anesthetics enflurane, halothane and the halothane metabolite trifluoroacetic acid with the binding of two highly bound acidic drugs (warfarin, phenytoin) to albumin has been studied in vitro by equilibrium dialysis. Trifluoroacetic acid (TfA) inhibited the binding of both drugs to human serum albumin (HSA). Halothane, on the other hand, increased the binding of warfarin to HSA, while enflurane inhibited only the binding of phenytoin. It seems that the binding of the acidic drugs warfarin and phenytoin to HSA is more sensitive to the structures of the gases than for the basic drug diazepam which was previously shown to be equally affected by both gases. furthermore, it seems that drugs competing for the same binding site (warfarin, phenytoin) may respond differently to conformation changes of the site. It is suggested that drugs bound to the "diazepam site" are more easily affected by the volatile anaesthetics than drugs bound to the "warfarin site".

关键词： HSA, human serum albumin K D , dissociation constant n , number of binding sites n/K D , binding capacity B , molar concentration of bound drug f , molar concentration of unbound drug P, molar concentration of protein MAC, minimum alveolar concentration TLC, thin layer chromatography TfA, tri-fluoroacetic acid

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fLOW-CYTOMETRY Of ISOLATED-CHLOROPLASTS AND THYLAKOIDS

PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS

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PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS 1986年第1-2期13卷 1-14页

作者： ASHCROfT, RG PRESTON, C CLELAND, RE CRITCHLEY, C ROYAL MELBOURNE HOSP LUDWIG INST CANC RES PARKVILLE VIC 3050 AUSTRALIA AUSTRALIAN NATL UNIV THE FAC DEPT BOT CANBERRA ACT 2601 AUSTRALIA

This paper describes the first flow cytometric measurements on plant leaf subcellular organelles. Intact chloroplasts and thylakoid membrane preparations from spinach and maize were assayed for forward and oblique light scatter and fluorescence emission. Chloroplast preparations showed at least two subgroups distinguished on light scatter and fluorescence, but thylakoid preparations had distinctly different properties. The fluorescence measured with single laser excitation was the constant yield component, f0. The variable fluorescence, fv, was detected by a dual laser technique. While the two types of spinach chloroplasts were almost identical in pigmentation, two of the maize mesophyll subgroups were quite different showing well separated emission maxima in the 685 and 710 nm bands.

关键词： flow cytometry Chloroplast Thylakoid Photosynthesis Wastewater Chlorophyll fluorescence Light scattering feCy ferricyanide pBQ phenyl-p-benzoquinone Chi chlorophyll f o constant yield fluorescence f v variable fluorescence fCM laser-activated flow cytometry PS II Photosystem II

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A TENTATIVE MODEL Of fORMATION Of STRUCTURAL PROTEINS Of TICK-BORNE ENCEPHALITIS-VIRUS (fLAVIVIRUS)

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fEBS LETTERS 1986年第2期200卷 314-316页

作者： LYAPUSTIN, VN SVITKIN, YV UGAROVA, TY LASHKEVICH, VA AGOL, VI ACAD MED SCI USSR INST POLIOMYELITIS & VIRAL ENCEPHALITISMOSCOWUSSR MOSCOW STATE UNIV MOSCOW 119899USSR

The time course of tick-borne encephalitis virus cell-free protein synthesis was studied by using either [35S]-methionine or formyl[35S]methionyl-tRNAMet/f as substrates, and the [35S]methionine-labelled products were compared by fingerprinting tryptic peptides. An intermediate in the protein processing, the polypeptide doublet p36/33, was characterized and a tentative model for flavivirus structural protein synthesis and processing was proposed.

关键词： flavivirus protein Cell-free protein synthesis Processing formyl 35 S methionyl-tRNA f Met

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IDENTIfICATION Of A 68-KDA PROTEIN WHICH COPURIfIES WITH TYPE-1 PROTEIN PHOSPHATASE AS ALBUMIN

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fEBS LETTERS 1986年第1期202卷 49-53页

作者： VILLAMORUZZI, E MEYER, HE HEILMEYER, LMG RUHR UNIV BOCHUM INST PHYSIOL CHEM 1D-4630 BOCHUMFED REP GER

Proteins of 60-70 kDa copurify with some preparations of type-1 or type-2 phosphatases. In our system chromatography on polylysine-Affi-Gel 10 separates a 68 kDa protein from rabbit muscle glycogen particle phosphorylase phosphatase. The separation affects neither the activity nor the size of the phosphatase. The 68 kDa protein, although pure by SDS gel electrophoresis criteria, still displays phosphatase activity of approx. 6-8 U/mg. However, rechromatography either on Bio-Gel A-0.5 m or on Blue Sepharose CL-6B followed by gel filtration shows that the activity is due to a contamination with phosphatases of type 1 and type 2, displaying a molecular mass of 35 kDa, which can be totally removed from the 68 kDa protein. The amino acid composition of the 68 kDa protein is identical to that of rabbit serum albumin, within the limits of variation for the method. furthermore, the sequence of the 38 N-terminal amino acids is the same in the isolated 68 kDa protein and in rabbit serum albumin.

关键词： Protein phosphatase Phosphorylase phosphatase Glycogen metabolism ( Muscle ) Albumin Amino acid composition N-terminal sequence analysis BSA, bovine serum albumin RSA, rabbit serum albumin PITC, phenylisothiocyanate f A , protein kinase that activates type-1 phosphatase DTT, dithiothreitol

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CORRELATION BETWEEN LINOLENIC-ACID DEfICIENCY IN CHLOROPLAST MEMBRANE-LIPIDS AND DECREASING PHOTOSYNTHETIC ACTIVITY IN BARLEY

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BIOCHIMICA ET BIOPHYSICA ACTA 1986年第1期849卷 77-84页

作者： LASKAY, G LEHOCZKI, E JOZSEF ATTILA UNIV DEPT BIOPHYS EGYETEM U2 H-6722 SZEGED HUNGARY

Barley plants having chloroplasts with a gradually diminished linolenic and a gradually increased linolenic acid content (a gradually reduced linolenic-to-linolenic acid ratio) were grown by the application of sublethal doses of the pyridazinone compound SAN 9785. The photosynthetic properties of the leaves and of the chloroplasts isolated from the leaves were studied. SAN 9785 caused only minor changes in the chlorophyll and total lipid contents of the chloroplasts. The liposomes prepared from the chloroplast membrane lipids exhibited a lower level of fluidity, as revealed by fluorescence polarization measurements using diphenylhexatriene. A strong correlation was established between the modified chemical composition of the thylakoid membranes (altered linoleic-to-linolenic acid ratio) and (i) the relative intensity of the short-wave-length fluorescence bands of the low-temperature emission spectra;(ii) the normalized increment of fluorescence (fi-fo)/(fm/fo) from reaction rate of isolated chloroplasts;and (v) the degree of coupling between electron transport and photophosphorylation. The results suggest that the presence of a sufficient amount of linolenic acid associated with chloroplast membrane lipid is essential for the functioning of the electron-transport chain and coupled phosphorylation. There seems to be a linolenic-to-linolenic acid ratio threshold value of 2 for satisfying coupling. SAN 9785 treatment also caused the chlorophyll-protein complex of the reaction centre of Photosystem I to decrease in quantity, suggesting a stabilizing role of the lipid environment in the assembly and organization of Photosystem-I particles.

关键词： Chloroplast fatty acid fluorescence induction Electron transport Membrane fluidity Pyridazinone (Barley chloroplast) SAN 9785 4-chloro-5-(dimethylamino)-2-phenyl-3(2H)-pyridazinone, also known as BASf 13.338 DPH 1,6-diphenyl-1,3,5-hexatriene 18:2 linoleic acid (number of carbon atoms/number of double bonds) 18:3 linolenic acid MGDG monogalactosyl diacylglycerol CP1 chlorophyll-protein complex of the reaction centre of Photosystem I CPa chlorophyll-protein complex of reaction centre of Photosystem II LHCP light-harvesting chlorophyll-protein complex PS I Photosystem I PS II Photosystem II f 0 fluorescence of constant yield f i initial yield of fluorescence f m maximal yield of fluorescence f -687 fluorescence bands at 687 nm f -695 fluorescence bands at 695 nm f -743 fluorescence bands at 743 nm Q first stable electron acceptor of Photosystem II PQ plastoquinone I 50 half-inhibitory concentration (in μM) D 9785 dose of SAN 9785 in mg/100 seedlings Chl chlorophyll

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EffECT Of TRIVALENT LANTHANIDE CATIONS ON CHLOROPHYLL fLUORESCENCE AND THYLAKOID MEMBRANE STACKING

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BIOCHIMICA ET BIOPHYSICA ACTA 1986年第2期851卷 322-326页

作者： KARUKSTIS, KK GRUBER, SM HARVEY MUDD COLL DEPT CHEM CLAREMONT CA 91711 USA

We have used trivalent lanthanide metal cations in the buffering media of pea chloroplast to probe the stacking arrangement of thylakoid membranes and the spatial distribution of chlorophyll-protein complexes of Photosystems I and II. Measurements of steady-state chlorophyll fluorescence emission spectra of pea chloroplasts at room temperature demonstrate that, within this tripositive valency group, the extent of membrane appression is a function of hydrated metal ionic radius. These results are in agreement with a recent investigation using monovalent and divalent metal cations (Karukstis, K.K. and Sauer, K. (1985) Biochim. Biophys. Acta 806, 374-389). In addition, the lanthanide cation concentration effective in producing the maximum chlorophyll fluorescence intensity upon grana formation is dependent on hydrated ionic size. The current investigation supports the proposed hypothesis that cation screening ability defines the extent of intermembrane separation as well as the extent of lateral distribution of chlorophyll-protein complexes.

关键词： Cation effect Chlorophyll fluorescence Thylakoid stacking (Pea chloroplast) Chl chlorophyll DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea f max maximum fluorescence level of chlorophyll fluorescence with Photosystem II electron acceptor Q reduced Hepes 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid LHC light-harvesting chlorophyll a b - protein complex PS I Photosystem I PS II Photosystem II

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