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RANDOMIZATION Of THE Ef PARTICLES IN THYLAKOID MEMBRANES Of SYNECHOCYSTIS-6714 UPON TRANSITION fROM STATE-I TO STATE-II

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fEBS LETTERS 1986年第2期208卷 308-312页

作者： OLIVE, J MBINA, I VERNOTTE, C ASTIER, C WOLLMAN, fA CNRS PHOTOSYNTH LABER 307F-91190 GIF SUR YVETTEFRANCE INST BIOL PHYS CHIM SERV PHOTOSYNTHF-75005 PARISFRANCE

In the cyanobacterium Synechocystis 6714 we show that changes in light energy distribution are controlled by the redox state of the PQ pool and we report on the deep ultrastructural modifications caused by these changes. We conclude that State I-State II transitions correspond to an increased energy transfer between PS II and PS I through a randomization of the Ef particles of the thylakoid membranes. This reorganization could involve a change in the interaction between PS II centers and the phycobilisomes.

关键词： Cyanobacteria State I-State II transition Thylakoid chlorophyll fluorescence freeze-fracture PQ, plastoquinone PS, photosystem LHC, light-harvesting complex Chl, chlorophyll DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea f v , variable fluorescence Ef, exoplasmic fracture face

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fLUORESCENCE INDUCTION TRANSIENTS INDICATE ALTERED ABSORPTION CROSS-SECTION DURING LIGHT-STATE TRANSITIONS IN THE CYANOBACTERIUM SYNECHOCOCCUS 6301

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BIOCHIMICA ET BIOPHYSICA ACTA 1986年第1期851卷 147-150页

作者： MULLINEAUX, CW BOULT, M SANDERS, CE ALLEN, Jf Department of Plant Sciences University of Leeds Leeds LS2 9JT U.K.

State 1-State 2 transitions in the cyanobacterium Synechococcus 6301 were observed using a lock-in amplifier to detect the fluorescence generated by a modulated excitation beam. Millisecond fluorescence induction transients were recorded for cells in State 1 and State 2. Comparison of the transients suggest that excitation energy distribution in this cyanobacterium is regulated by changes in the absorption cross-section of Photosystem II.

关键词： Photosynthesis State 1–State 2 transition Chlorophyll fluorescence Light-state adaptation ( Synechococcus sp.) DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea LHC II light-harvesting chlorophyll a b protein complex PS Photosystem PQ plastoquinone f 0 initial level of fluorescence f m maximal level of fluorescence f t fluorescence at time t light 1 a light preferentially exciting PS I light 2 a light preferentially exciting PS II

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LATERAL DISTRIBUTION Of THE MAIN PROTEIN COMPLEXES Of THE PHOTOSYNTHETIC APPARATUS IN CHLAMYDOMONAS-REINHARDTII AND IN SPINACH - AN IMMUNOCYTOCHEMICAL STUDY USING INTACT THYLAKOID MEMBRANES AND A PS-II ENRICHED MEMBRANE PREPARATION

PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS

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PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS 1986年第3-4期12卷 203-220页

作者： VALLON, O WOLLMAN, fA OLIVE, J UNIV PARIS 07 CNRSINST JACQUES MONODMICROSCOPIE ELECTR LAB 2 PL JUSSIEU F-75251 PARIS 05 FRANCE INST BIOL PHYS CHIM F-75231 PARIS 05 FRANCE

The localization of several polypeptides of the thylakoid membrane was investigated, using immunogold labeling of Lowicryl sections of spinach chloroplast and broken cells of Chlamydomonas reinhardtii. The herbicide receptor protein D1 and polypeptide D2, both associated with the Photosystem II reaction center were mostly found in stacked membranes (90%), as was the case for the main subunit of the light-harvesting complex. Antigens belonging to Photosystem I or to the coupling factor 1 of the ATPase were located exclusively in unstacked regions, whereas ferredoxin NADP reductase was found not totally restricted to these regions. Immunogold labeling of a Photosystem II preparation from spinach (Berthold, D.A., G.T. Babcock and C.f. Yocum (1981) fEBS Lett. 134, 231-234) showed contamination by Photosystem I only at the periphery of the stacked membrane sheets. In contrast, all the other protein constituents examined, including the cytochrome b6/f complex, were distributed all over the fragments.

关键词： thylakoid membrane Chlamydomonas Photosystem I Photosystem II light-harvesting complex ferredoxin-NADP reductase cytochrome b 6/ f chloroplastic ATPase immunocytochemistry α antibody to … BBY PS II enriched preparation according to Berthold, Babcock and Yocum Cfl ATPase coupling factor l CP chlorophyll-protein complex cyt b 6/ f cytochrome b 6/ f complex fNR ferredoxin-NADP reductase LHC light-harvesting complex PS I Photosystem I PS II Photosystem II TMBZ 3,3',5,5'-tetramethylbenzidine Tris tris-(hydroxymethyl)-amino methane SOS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis

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PROLINE ISOMERIZATION DURING REfOLDING Of RIBONUCLEASE-A IS ACCELERATED BY THE PRESENCE Of fOLDING INTERMEDIATES

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fEBS LETTERS 1986年第2期198卷 217-220页

作者： SCHMID, fX Institut für Biophysik & Physikalische Biochemie Universität Regensburg D-8400 Regensburg FRG

The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had been converted to a folding intermediate by a 15 s refolding pulse under strongly native conditions (0.8 M ammonium sulfate, 0 degree C). The results demonstrate that extensive folding is possible before Pro 93 isomerizes to its native cis state and that the presence of structural folding intermediates leads to a marked increase in the rate of subsequent proline isomerization.

关键词： Protein folding Ribonuclease A folding intermediate Proline isomerization RNase A, bovine pancreatic ribonuclease A (EC 3.1.27.5) N, native protein U f and U S species, fast- and slow-refolding species of unfolded RNase, respectively I 1 ,I N , folding intermediates GdmCl, guanidinium chloride

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EffECT Of HOMOGENEOUS CATALYTIC-HYDROGENATION Of MEMBRANE-LIPIDS ON LUMINESCENCE CHARACTERISTICS Of THE PHOTOSYSTEM-II ELECTRON-TRANSPORT

PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS

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PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS 1986年第3-4期12卷 221-230页

作者： HIDEG, E ROZSA, Z VASS, I VIGH, L HORVATH, G HUNGARIAN ACAD SCI BIOL RES CTR INST PLANT PHYSIOL POB 521 H-6701 SZEGED HUNGARY HUNGARIAN ACAD SCI BIOL RES CTR INST BIOCHEM H-6701 SZEGED HUNGARY

In situ homogenous catalytic hydrogenation of fatty acyl chains of membrane lipids was applied to study the effect of the saturation of lipids on photosynthetic electron transport within Photosystem II. We found that hydrogenation increased the level of intermediate fluorescence without affecting the maximal level of fluorescence. There was no significant change in the total intensity of thermoluminescence, however, the individual bands of thermoluminescence were altered by hydrogenation. Comparing the characteristics of fluorescence induction and thermoluminescence that catalytic hydrogenation decreases the electron transfer between the "primary" electron acceptor QA and the "secondary" acceptor QB.

关键词： chloroplast fluorescence induction homogeneous catalytic hydrogenation membrane-lipid photosynthesis Photosystem II thermoluminescence DCMU 3-(3', 4'-dichlorophenyl)-1,1-dimethylurea f 0 non-variable fluorescence f i intermediate fluorescence yield f max total fluorescence yield f var variable fluorescence yield pBQ p-benzoquinone Pd(QS) 2 Pd(II) sulphonated alizarine complex PS Photosystem Q A and Q B “primary” and “secondary” acceptors of Photosystem TL thermoluminescence

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Modelling the Characteristics of Thermal Power Plant Boiler furnaces

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IfAC Proceedings Volumes 1986年第16期19卷 201-207页

作者： L.N. Patel Department of Mechanical Engineering S. V. Regional College of Engineering & Technology Surat-395007 India

The performance of the thermal plant depends upon the efficiency of each component encountered in it. The furnace which is expected to transfer heat from hot gases to sink surface is the heart of such a plant. Efficient furnace can be designed on the basis of accurate estimation of heat transfer. Due to inadequate data on fluid flow, heat transfer and combustion processes, even today no complete analytical procedure has been established either to design a new furnace or investigate the performance characteristics in case of an existing furnace. To handle this complex problem, combustion engineer prefers to first model the furnace characteristics and then optimize the design parameter. It would be either too expensive or sometimes perhaps Impossible to obtain the same information experimentally. The objective of this article is to present a generalized procedure for modelling the characteristics of boiler furnace and compare the results obtained tram the model equations with the experimental data of the same furnace. Large number of furnace parameters such as shape and size of furnace, airfuel data, operating conditions, radiative properties of hot gases and those of tube walls, environmental conditions etc. make it too tedious to deal with the furnace model. To tackle the problem, a generalized computer program has been developed. The effects of various radiative properties on the performance of the model can be investigated conveniently by computer interaction.

关键词： A surface area E emissive power f shape factor H heat flux incident h enthalpy, heat transfer coefficient M number of surfaces forming a zone m mass N number of zones Q heat transfer rate T temperature W radiosity ∊ emissivity ϱ reflectivity τ transmissivity ϕ equivalence ratio 1 re-radiating surface 2 heat sink surface b burning, combustion, black surface bg black gas fL flame g gas i ith surface j jth surface ij between i and j i,j second order subscript (e.g. Q r, i means radiation heat transfer for ith surface) (m) mth zone (n) nth imaginary surface (n,m) nth imaginary surface of mth zone r radiation w wall

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MITOCHONDRIAL RESPIRATORY CONTROL IN THE MYOCARDIUM

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BIOCHIMICA ET BIOPHYSICA ACTA 1986年第2期853卷 135-151页

作者： HASSINEN, IE Department of Medical Biochemistry University of Oulu Oulu Finland

The heart muscle has proved to be a practical model for studying respiratory control in intact tissues. It also demonstrates that control at the level of the respiratory chain is augmented by metabolic control at the substrate level as exemplified by the very narrow range of changes in the redox state of the mitochondrial NADH/NAD couple even during extensive changes in ATP and oxygen consumption. The behaviour of mitochondria when isolated can largely be duplicated in the intact myocardium. Moreover, the high intracellular concentrations of enzymes, coenzymes and adenine nucleotides create conditions of high reaction rates, enabling the formation of a near equilibrium network of certain main pathways. This equilibrium network in connection with metabolic regulation of the hydrogen pressure upon the matrix NADH/NAD pool is a prerequisite for the regulation of cellular respiration at a high efficiency of energy transfer. Experimentation on the intact myocardium also seems to be capable of resolving some of the uncertainties about prevailing mechanisms for the regulation of cellular respiration.

关键词： Δϕ mitochondrial membrane potential free energy change in ATP hydrolysis electrochemical potential difference of protons across the mitochondrial inner membrane CrP creatine phosphate Cr creatine E h actual redox potential ΔE h actual potential difference between two redox couples ΔΦ diffusion potential imposed by metabolite distribution ΔG or free energy change in an oxidation-reduction reaction f the faraday constant R the gas constant K d binding site-ligand dissociation constant Glut glutamate Asp aspartate ETf electron-transferring flavoprotein

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RHODAMINE-123 - PHOTOPHYSICAL AND PHOTOCHEMICAL PROPERTIES

PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS

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PHOTOBIOCHEMISTRY AND PHOTOBIOPHYSICS 1986年第2期11卷 139-148页

作者： CHOW, A KENNEDY, J POTTIER, R TRUSCOTT, TG ROYAL MIL COLL CANADA DEPT CHEM & CHEM ENGN KINGSTON K7K 5LO ONTARIO CANADA QUEENS UNIV DEPT RADIAT ONCOL KINGSTON K7L 3N6 ONTARIO CANADA

The ground state absorbance and the fluorescence emission spectra of rhodamine 123 are reported as a function of pH and dye concentration. Corresponding results are presented on the triplet state using the laser flash photolysis technique. Complete conversion of the dye to the triplet state is observed at relatively low concentrations despite the high quantum yield of fluorescence (0.9) which is due to strong reabsorption. The relevance of these results to the recently reported anticarcinoma activity of this well-known laser dye is discussed.

关键词： rhodamine 123 photosensitization fluorescence triplets Rh 123 rhodamine 123 ∅ f fluorescence quantum yield ∅ T triplet quantum yield ΔOD difference optical density Δϵ difference absorption coefficient

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TAIL AREA REVISITED

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COMMUNICATIONS IN STATISTICS-SIMULATION AND COMPUTATION 1986年第4期15卷 1215-1234页

作者： AWAD, AM SHAYIB, MA KUWAIT UNIV DEPT MATHSAFAT 13060KUWAIT

This paper gives a wider generization to, and has evolved from, Andrews' article. The suggested approximating formulae are applicable on more distributions than was considered by Andrews. The family of distributions for which the approximated area equals to the exact area is included. Our numerical values are more precise than those of Andrews because his work was done in an era when computing facilities were not as well developed as they are now. The results derived here are applied to several examples. Moreover, some numerical calculations are given to compare the relative errors of the suggested approximating formulae.

关键词： approximations nonmal t chi-square f log-normal logistic extreme value stable log-Cauchy Cauchy exponential and Pareto distributions

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Topographic Histochemstry of the Cerebellum: 5′-Nucleotidase, Acetylcholinesterase, Immunology of fal

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Progress in Histochemistry and Cytochemistry 1986年第4期16卷 3-10页

作者： Marani, Enrico Anatomisch-Embryologisch Laboratorium Rijksuniversiteit Leiden Leiden 2300 RC Wassenaarseweg 62 Postbus 9602 Netherlands

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