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KINETIC AND SPECTRAL RESOLUTION Of CYTOCHROME-C-553 AND CYTOCHROME-f IN THE PHOTOSYNTHETIC ELECTRON-TRANSfER CHAIN Of HETEROCYSTS

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fEBS LETTERS 1983年第1-2期159卷 267-270页

作者： HOUCHINS, JP HAWKESfORD, MJ HIND, G Biology Department Brookhaven National Laboratory Upton NY 11973 USA

Oxidation of cytochrome c -553 and cytochrome f following single-turnover flashes was measured in isolated heterocysts. Half-times of oxidation for these two cytochromes were determined by measuring the rate of bleaching at 554 nm in heterocysts possessing cytochrome c -553, or in heterocysts in which cytochrome c -553 was replaced by plastocyanin. flash-induced difference spectra demonstrate that a fast bleaching ( t 0.5 = 20–35 μs) corresponds to the oxidation of cytochrome c -553, and a slower bleaching ( t 0.5 = 87 μs) corresponds to the oxidation of cytochrome f .

关键词： Heterocyst Cytochrome f Cytochrome c -553 Plastocyanin Anabaena Photosynthetic electron transfer

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LACK Of ABILITY Of TRYPSIN-TREATED MITOCHONDRIAL f1-ATPASE TO BIND THE OLIGOMYCIN-SENSITIVITY CONfERRING PROTEIN (OSCP)

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fEBS LETTERS 1983年第1期162卷 5-10页

作者： HUNDAL, T NORLING, B ERNSTER, L Department of Biochemistry Arrhenius Laboratory University of Stockholm S-106 91 Stockholm Sweden

Soluble beef-heart mitochondrial f1-ATPase modified in its alpha-subunit by mild trypsin treatment (alpha'-f1) can no longer bind oligomycin-sensitivity conferring protein (OSCP) but is still capable of binding to f1-depleted submitochondrial particles, giving rise to a maximally oligomycin-sensitive ATPase, provided the particles contain their native complement of OSCP. When OSCP is removed from the particles, alpha'-f1 can still bind to the particles, but added OSCP induces only a low degree of oligomycin sensitivity. The possible role of OSCP in the functional coupling of the catalytic (f1) and H+-translocating (fo) moieties of mitochondrial ATPase is discussed. The results suggest a functional similarity between the OSCP component of mitochondrial ATPase and the delta-subunit of E. coli ATPase, which is in accordance with the structural homology recently found to exist between the two polypeptides.

关键词： Oligomycin-sensitive ATPase f 1 -ATPase f 1 -subunit Oligomycin-sensitivity conferring protein Reconstitution Trypsin digestion f o and f 1 proton-translocating and catalytic moieties of the mitochondrial ATPase system OSCP oligomycin-sensitivity conferring protein f 6 coupling factor 6 SDS sodium dodecyl sulfate

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PHOTOAffINITY-LABELING Of CHLOROPLAST CYTOCHROME-B6-f COMPLEX BY AN INHIBITOR AZIDO-DERIVATIVE

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fEBS LETTERS 1983年第2期155卷 241-244页

作者： OETTMEIER, W MASSON, K OLSCHEWSKI, E Lehrstuhl Biochemie der Pflanzen Ruhr-Universität Postfach 10 21 48 4630 Bochum 1 FRG

The photoaffinity label 2-azido-2′,4,4′-trinitro-6- sec. butyldiphenylether inhibits photosynthetic electron transport in the thylakoid membrane as well as plastohydroquinone-plastocyanin-oxidoreductase activity in the isolated chloroplast cytochrome b 6 - f complex. In the latter, the 14 C-labeled inhibitor upon UV-irradiation binds covalently only to the cytochrome b 6 and the Rieske fe-S peptide subunit.

关键词： Photoaffinity label Cytochrome bin6 - f complex Inhibitor Plastoquinone Rieske fe-S protein Cytochrome b 6

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CYTOCHROME-B6 fROM ISOLATED CYTOCHROME-B6f COMPLEXES - EVIDENCE fOR 2 SPECTRAL fORMS WITH DIffERENT MIDPOINT POTENTIALS

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fEBS LETTERS 1983年第2期153卷 413-419页

作者： HURT, EC HAUSKA, G Universität Regensburg Institut für Botanik D-8400 Regensburg FRG

Cytochrome b 6 from spinach chloroplasts (either within the purified cytochrome b 6 f complex, or in its isolated form) exhibits two spectral species, which correspond to two midpoint potentials. This can be demonstrated by low temperature difference spectroscopy at fixed redox potentials. The high potential form of cytochrome b 6 has a split α-peak at 557.5 and 561.5 nm, the low potential form has a symmetrical α-peak at 560.5 nm. Similar results were obtained with cytochrome b 6 in the isolated cytochrome b 6 f complex from the cyanobacterium Anabaena variabilis .

关键词： Cytochrome b 6 Cytochrome b 6 f complex Chloroplast electron transport Cyanobacteria Low temperature difference spectroscopy Redox potential MOPS 3-( N -morpholino)propanesulfonic acid MES 2-( N -morpholino)ethanesulfonic acid Tris Tris(hydroxymethyl) aminomethane

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由1/f=1/u+1/v 所联想到的平面几何作图题

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中等数学 1983年第1期 40-42页

作者：韩健南开中学82届毕业生

在学习光学时,我们遇到这样一个公式——1/f=1/u+1/v,其中 f 为焦距,u、v 分别为物距和象距。若己知主光轴、透镜的位置,以及物距 u 和象距 v,可以通过作图求出焦点和焦距,作法见图1。由此物理题出发,可以用这种作图方法解决下面的平面... 详细信息

在学习光学时,我们遇到这样一个公式——1/f=1/u+1/v,其中 f 为焦距,u、v 分别为物距和象距。若己知主光轴、透镜的位置,以及物距 u 和象距 v,可以通过作图求出焦点和焦距,作法见图1。由此物理题出发,可以用这种作图方法解决下面的平面几何作图题。

关键词：平面几何 f=1/u+1/v f 作图题

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PRODUCTION Of AUTO-ANTI-IDIOTYPIC ANTIBODY DURING THE NORMAL IMMUNE-RESPONSE .6. HAPTEN AUGMENTATION Of PLAQUE-fORMATION AND HAPTEN-REVERSIBLE INHIBITION Of PLAQUE-fORMATION AS ASSAYS fOR ANTI-IDIOTYPE ANTIBODY

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JOURNAL Of IMMUNOLOGICAL METHODS 1983年第1-2期58卷 1-17页

作者： GOIDL, EA HAYAMA, T SHEPHERD, GM SISKIND, GW THORBECKE, GJ NYU DEPT PATHOL NEW YORK NY 10016 USA CORNELL UNIV MED CTR COLL MED DIV ALLERGY & IMMUNOL NEW YORK NY 10021 USA

Evidence was presented that the detection of hapten-augmentable plaques indicates cells in which secretion of antibody had been blocked by the binding of auto-anti-id [anti-idiotype antibody] to cell surface idiotypes. Because of the dependence of the assay on the affinities of the various species for one another, the number of hapten-augmentable plaques detected should be regarded as a minimal estimate of the number of cells in which secretion of antibody is inhibited by auto-anti-id. for confirmation that hapten-augmentable PfC [plaque forming cells] are due to auto-anti-id, 2 principal controls are important: incubation of the spleen cell population with hapten prior to plaquing should remove the hapten-augmentable PfC;and the dialyzed supernate from hapten incubated cells should inhibit plaque formation in a hapten-reversible manner. Evidence also is presented that hapten-reversible inhibition of plaque formation can serve as an assay for anti-id. Apparent false-positive assays can result from the presence of anti-hapten antibody or antigen-antibody complexes;these apparent false positives are rarely reversed by hapten. Removal of anti-hapten antibody, by passage over an antigen immunoadsorbent, will eliminate this source of false positives, and the procedure is recommended. false-negative results can arise from mismatching of the anti-id in the sample to be assayed and the idiotypes of the target cells used in the assay. This can result from shifts in idiotype expression related to age and time after antigen injection. false negatives can also result from the presence of idiotype-anti-id complexes in the sample to be assayed. This source of false negatives can sometimes be eliminated by passage of the sample through an antigen immunoadsorbent. The existence of a high degree of hapten-augmentable PfC introduces complexities into the interpretation of hapten inhibition of plaque formation in terms of the affinity of the antibody secreted by the PfC. In such cases

关键词： hapten augmentation of plaque forming cells auto-anti-idiotypic antibodies hapten reversible inhibition of plaque forming cells anti-id anti-idiotype antibody Ars arsonate group CRI cross-reactive idiotype DNP 2,4-dinitrophenyl group f ficoll HBSS Hanks' balanced salt solution Ig immunoglobulin PfC plaque forming cell(s) SRBC sheep erythrocytes TNP 2,4,6-trinitrophenyl group TNP-f trinitrophenylated-lysyl-ficoll

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ANALYSIS Of CORTICOSTEROIDS IN HUMAN ADRENAL-TISSUE BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

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CLINICA CHIMICA ACTA 1983年第3期131卷 239-246页

作者： SAITO, Z MIMOH, N HIfUMI, S TAKEDA, R Second Department of Internal Medicine School of Medicine Kanazawa University 13-1 Takara Machi Kanazawa City 920 Ishikawa Prefecture JapanReceived 12 October 1982 Revised 3 March 1983 Available online 15 January 2003 Show moreChoose an option to locate/access this article:Check if you have access through your login credentials or your institution. Check access Sign in using your ScienceDirect credentialsUsername:Password: Remember meNot Registered? Forgotten username orpassword? OpenAthens login Login via your institution Other institution login Remote access activation Click here to activate

High pressure liquid chromatography (HPLC) was demonstrated to be a goood tool for the separation, identification and quantitation of corticosteroids (CS) extracted from homogenized tissue of adrenal glands and adrenal tumors in patients with hypercorticism. A chromatographic system consisting of Sorbax-SIL and Sorbax-CN columns, organic solvent extraction and a UV detector was used to analyze both more polar and less polar corticosteroids in the adrenal tissue.

关键词： P progesterone DOC 11-deoxycorticosterone S 11-deoxycoitisol E cortisone A aldosterone f cortisol 18-OH-B, 18-hydroxy-corticosterone 6β-OH-f 6β-hydroxycortisol 20-OH-f 20-hydroxycortisol and 20-epi-OH-f 20-epi-hydroxycortisol

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ENHANCEMENT Of CHEMOTACTIC MIGRATION BY THE LOCAL-ANESTHETIC TETRACAINE

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IMMUNOBIOLOGY 1983年第1期165卷 97-106页

作者： KOWNATZKI, E UHRICH, S Abteilung Experimentelle Dermatologie Universit?ts-Hautklinik Freiburg Federal Republic of GermanyReceived 2 February 1983 Accepted 22 March 1983 Available online 2 November 2011

The local amine anesthetic tetracaine added to a suspension of guinea pig or human neutrophilic granulocytes inhibited their random migration in Boyden chambers but increased their chemotactic migration towards the chemotactic tripeptide f-Met-Leu-Phe, complement-activated normal guinea pig serum and the eosinophil chemotactic factor (ECf). Tetracaine not only increased the distance migrated by the leading cells but also caused more cells to leave the upper filter surface and to migrate into the filter. The effect required the presence of the drug;cells preincubated with tetracaine and washed did not differ from control cells. Tetracaine may specifically enhance a mechanism operative in a cell''s response to a concentration gradient of a chemotactic factor.

关键词： BSA bovine serum albumin f - MLP formyl-methionyl-leucyl-phenylalanine ECf eosinophil chemotactic factor HBSS Hanks' balanced salt solution HBSS - BSA HBSS containing 5 mg/ml BSA NGS normal guinea pig serum NGS - Y yeast-activated NGS PMN polymorphonuclear granulocytes

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CHARACTERIZATION Of IDIOTOPES ON MOPC-315 IGA USING MONOCLONAL ANTIIDIOTYPIC ANTIBODIES

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MOLECULAR IMMUNOLOGY 1983年第5期20卷 537-547页

作者： NUSAIR, T BAUMAL, R ROSENSTEIN, R JORGENSEN, T MARKS, A UNIV TORONTO CHARLES H BEST INST BANTING & BEST DEPT MED RES TORONTO M5G 1L6 ONTARIO CANADA HOSP SICK CHILDREN DEPT PATHOL TORONTO M5G 1X8 ONTARIO CANADA YALE UNIV SCH MED DEPT PATHOL NEW HAVEN CT 06510 USA UNIV TROMSO SCH MED INST MED BIOL N-9001 TROMSO NORWAY

The isologous antiidiotypic response in BALB/c mice to immunization with the DNP[dinitrophenyl]-binding IgA myeloma protein, MOPC 315, alters the expression of the anti-DNP antibody repertoire and confers immunity against MOPC 315 myeloma tumors. To characterize the idiotopes on MOPC 315 IgA which elicit this response 4 monoclonal antiidiotypic antibodies (AIA), D10 (IgG2a), A2 (IgG1), G3 (IgG2b) and f1 (IgG2a), produced by splenocytes of BALB/c mice immunized with MOPC 315 IgA were isolated in 3 independent fusion experiments. These AIA react with MOPC 315 IgA, reassociated H315 L315 and .**GRAPHIC**. but not with free H315, L315, .**GRAPHIC**. or .**GRAPHIC**. The AIA do not react with the closely related DNP-binding IgA myeloma protein, MOPC 460, suggesting that they are directed against private idiotypes on MOPC 315 IgA. These idiotopes can be divided into 2 groups. Group I, defined by D10, A2 and G3, consists of 2 overlapping idiotopes, 1 of which is related to the hapten-binding site. The 2 idiotopes are formed by an interaction of amino acids in H315 and L315. Group II, defined by f1, consists of 1 idiotope which is related to the hapten-binding site. This idiotope is composed of an amino acid sequence on H315 which requires an interaction with either L315 or L460 for expression. A2 and G3 react identically with the same idiotope but were derived from 2 independent fusion experiments. An identity of AIA clonotypes among individual mice was indicated and the isologous AIA response to MOPC 315 IgA may be restricted.

关键词： AIA antiidiotypic antibody CfA complete freund's adjuvant DNP 2,4 dinitrophenyl DNP-cap N -2,4-DNP-ϵ-amino-caproic acid fCS fetal calf serum KLH keyhole limpet hemocyanin PBS phosphate buffered saline (140 m M NaCl, 3 m M KCl, 0.15 m M KH 2 PO 4 , 8 m M Na 2 HPO 4 ·7H 2 O, pH 7.4) Ig immunoglobulin H heavy L light L 315 L 460 free L chains of MOPC 315 460 IgA respectively H 315 H 460 free H chains of MOPC 315 460 IgA respectively f 315 V fragment consisting of V domains of MOPC 315 IgA V 315 2 fragment consisting of V domains of L 315 V 315 H fragment consisting of V domains of H 315

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EffECTS Of 3-METHYLCHOLANTHRENE ON COVALENT BINDING AND TOXICITY Of 4-IPOMEANOL IN INDUCIBLE AND NON-INDUCIBLE (B6D2) MICE

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TOXICOLOGY 1983年第3期28卷 183-191页

作者： JONES, RB STATHAM, CN BOYD, MR NCI DIV CANC TREATMENTDEV THERAPEUT PROGRAM EXPTL THERAPEUT & METABOL LABBLDG 37ROOM 5B22 BETHESDA MD 20205 USA

The effects of 3-methylchloanthrene (MC) on the covalent binding and toxicity of 4-ipomeanol (1-(3-furyl)-4-hydroxypentanone, IPO) were studied in (C57BL/6N)(DBA/2N)f1 x DBA/2N backcross (B6D2)D2 mice previously segregated into relatively inducible or noninducible groups based on zoxazolamine (2-amino-5-chlorobenzoxazole, ZOX) paralysis times following MC treatment. MC decreased the covalently bound IPO metabolite(s) in the lungs and kidneys of inducible and noninducible mice when compared to controls not pretreated with MC. Concentrations of covalently bound IPO metabolite(s) in liver were increased in inducible mice and decreased in noninducible mice by MC pretreatment when compared to nonpretreated heterogenous mice. Associated with MC pretreatment was a significant decrease in the acute lethality of IPO in the inducible and noninducible mice when compared to nonpretreated control animals (LD50: 213 .+-. 2, 140 .+-. 14 and 14 .+-. 4 mg/kg, respectively). Hepatic necrosis occurred frequently in the inducible mice and occasionally in the noninducible mice given large IPO doses near the respective LD50-values. Hepatic necrosis was never observed in nonpretreated mice receiving near lethal doses of IPO. These results supported previous studies indicating that reactive IPO metabolites binding to extrahepatic tissues were formed in situ and did not reflect binding of blood-borne metabolites formed in the liver.

关键词： (B6D2)D2 ( C 57 BL 6 N )( DBA 2 N ) f 1 × DBA 2 N backcross IPO 4-ipomeanol MC 3-methylcholanthrene ZOX zoxazolamine

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