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BINDING Of INULO-OLIGOSACCHARIDES TO HOMOGENEOUS IMMUNOGLOBULINS-E109 AND IMMUNOGLOBULIN-A47N

MOLECULAR IMMUNOLOGY 1979年第2期16卷 97-100页

作者： DAS, MK STREEfKERK, DG GLAUDEMANS, CPJ NIAMDD BETHESDAMD 20014

Inulo-biose, -triose and -tetraose have been separated from, respectively, sucrose, 1-kestose and nystose by chromatography. The binding of these oligosaccharides has been studied with murine anti-inulin myeloma immunoglobulins E 109 and A47N. from these and previously obtained data it is proposed that there is a sub-site in these two anti-inulins which binds a fructose unit much more strongly than any other sub-site, and that any oligosaccharide ligand will fill that sub-site at a higher rate.

关键词： H heavy L light fruƒ fructofuranosyl fG 1 sucrose me fruƒ methyl β- d -fructofuranoside me Glc p methyl α- d glucopyranoside f 4 g 1 1 β- d -fruƒ (2[→1)-β- d -fruƒ(2] 3 →1)-α- d -Glc p Tris trishydroxymethylamino methane

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DIffERENTIAL SENSITIVITY Of ISOLATED RAT THYMOCYTES TO GLUCOCORTICOIDS fOLLOWING CONCANAVALIN-A STIMULATION

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JOURNAL Of STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 1979年第6期10卷 581-585页

作者： CIDLOWSKI, NB CIDLOWSKI, JA Department of Biochemistry University of Vermont College of Medicine Burlington VT 05405 U.S.A.

Concanavalin A (Con A) rapidly stimulates Leu incorporation into trichloroacetic acid (TCA) precipitable, but not TCA soluble material in isolated rat thymocytes. This stimulation of Leu incorporation by Con A is dose dependent, time dependent and is inhibited by the addition of cycloheximide. In non-mitogen stimulated thymocytes, cortisol (10-6 M) inhibits Leu incorporation into protein by 30%, whereas in Con A-stimulated cells, cortisol inhibits Leu incorporation by 50%. The cortisol effect in Con A-stimulated cells is present when cortisol and Con A are added simultaneously, is diminished when cortisol is added at intervals after Con A and is dependent on the concentration of cortisol administered. The inhibitory effect of cortisol on Leu incorporation is dose and time dependent in both control and Con A treated cells. The magnitude of the cortisol effect does not differ in control and Con A-stimulated thymocytes for the first 3 h of steroid exposure, after which time a significant increase in the glucocorticoid effect occurs only in the Con A-stimulated cells. This increased response to cortisol occurs at a time which is coincident with the mean maximal response of these cells to Con A alone. Both progesterone and cortexolone (10-6 M) inhibit Leu incorporation in control cells only slightly. The magnitude of these effects does not change by stimulation of the thymocytes with Con A. Under the conditions of these experiments, the rate of accumulation and the levels of nuclear glucocorticoid receptors are the same in both and Con A-stimulated thymocytes. Thymocyte responsiveness to glucocorticoid is evidently increased following exposure to Con A. This enhanced response to glucocorticoid probably occurs subsequent to nuclear accumulation of steroid receptor complexes.

关键词： f cortisol (11β 17α 21 trihydroxy-4-pregnene-3 20-dione) cortisone (17α 21-dihydroxy-4-pregnene-3 11 20 trione) corticosterone (11β 21-dihydroxy-4-pregnene-3 20 dione) cortexolone (17α 21-dihydroxy-4-pregnene-3 20-dione) progesterone (4-pregnene-3 20-dione) MEM-L minimum essential medium without leucine Con A concanavalin A

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PHOTOSYNTHESIS Of IVY LEAVES (HEDERA-HELIX L) AfTER HEAT-STRESS .3. STOMATAL BEHAVIOR

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ZEITSCHRIfT fUR PfLANZENPHYSIOLOGIE 1979年第3期92卷 277-283页

作者： BAUER, H Institut für Allgemeine Botanik der Universität Innsbruck Austria

Stomatal responses to light/dark-changes and exposure to various ambient CO 2 -concentrations of previously heat-stressed (30 min at 44, 46, or 48 °C) leaves of Hedera helix L. were studied. Stomatal conductance (k′ s ) and the intercellular CO 2 -concentration (c′ i ) were calculated from simultaneous measurements of transpiration and CO 2 -gas exchange in an open system. Stomatal responses to changes in c′ i were delayed during the first hours after heat stress and stomatal opening in light and closure in darkness were incomplete. 24 hours after stress, the stomata were able to react again as fast as before the stress, but k− s in light was still reduced. When the leaves were flushed with CO 2 poor air, however, the stomata opened almost completely. This indicates that the reduction of k− s after heat stress was caused by the raised c′ i due to reduced photosynthetic CO 2 uptake. As long as photosynthesis was impaired after heat stress, the CO 2 -sensitivity of the stomata was abnormal: related to the same c′ i , the stomata were open wider in heat-stressed leaves than in non-stressed ones.

关键词： heat stress stomatal conductance intercellular CO 2 -Concentration k a , k′ a conductance of the boundary layer for water vapour and CO 2 , respectively (cm S −1 ) k s , k′ s stomatal conductance for water vapour and CO 2 , respectively (cm s −1 ) c a , c− a concentration of water vapour and CO 2 , respectively, in the ambient air (μg cm −3 ) c i , c′ i concentration of water vapour and CO 2 , respectively, in the intercellular spaces (μg cm −3 ) Tr transpiration (μg cm −2 s −1 ) f n net photosynthesis (μg cm −2 s −1 )

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The biogenesis of rat liver mitochondrial ATPase. Subunit composition of the normal ATPase complex and of the deficient complex formed when mitochondrial protein synthesis is blocked.

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Biochimica et biophysica acta 1979年第1期548卷 48-62页

作者： de Jong L. Holtrop M. Kroon A.M. Laboratory of Physiological Chemistry State University Bloemsingel 10 9712 KZ Groningen The Netherlands

1. An ATPase complex containing 12 subunits was isoalted from rat liver mitochondria. 2. In vivo inhibition of mitochondrial protein synthesis by the chloramphenicol analogue thiamphenicol leads to the formation of an oligomycin-insensitive membrane-bound ATPase complex in mitochondria of regenerating rat liver. 3. This oligomycin-insensitive, membrane-bound ATPase was isolated by the same procedure as the ATPase complex from regenerating livers of untreated animals. 4. SDS-polyacrylamide gel electrophoresis of in vivo labelled ATPase complexes from control and from thiamphenicol-treated rats reveals that three subunits out of the 12 are not synthesized or assembled when the mitochondrial translation activity is blocked. 5. from the subunits synthesized and assembled when mitochondrial pror (fo) of the ATPase complex (subunit 5). 6. The oligomycin sensitivity-conferring protein seems absent in the ATPase complex formed in the presence of thiamphenicol.

关键词： Mitochondrial ATPase Regeneration Oligomycin-sensitive Thiamphenicol Subunit composition Protein synthesis inhibition (Rat liver) f 1 coupling factor I (soluble oligomycin-insensitive ATPase) f o coupling factor o (the membrane part of oligomycin-sensitive ATPase) f c2 coupling factor c 2 (part of f o ) OSCP oligomycin sensitivity-conferring protein (part of f o ) Hepes N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid DCCD N , N ′-dicyclohexylcarbodiimide SDS sodium dodecyl sulfate

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The amino-terminal sequence of the 40 000 molecular weight subunit of the acetylcholine receptor protein from Torpedo marmorata

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fEBS Letters 1979年第1期104卷 99-105页

作者： Devillers-Thiery, Anne Changeux, Jean-Pierre Paroutaud, Pierre Strosberg, A.Donny Neurobiologie Moléculaire and Laboratoire Associé au Centre National de la Recherche Scientifique Interactions Moléculaires et Cellulaires Paris France Unité d'Immunologie Moléculaire IRBM Université Paris VII France Biochemical Pathology Free University Brussels Belgium

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The hydroxylapatite-column assay of estrogen receptors: The routine analyses of many samples and the calculation of the equilibrium association constant

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Journal of Steroid Biochemistry 1979年第3期10卷 267-275页

作者： Erdos, T. Bessada, R. Laboratoire d'Enzymologie C.N.R.S. 91190 Gif-sur-Yvette France

The hydroxylapatite-column assay of estrogen receptors, described previously [Erdos et al. Analyt. Biochem. 37 244 (1970)], has been modified for the routine analyses of many samples. Sixty assays can be performed in 4h with the help of home-made filtering devices or even faster on commercial filter-racks. The method is at least as simple and rapid as its "batch" variant, it is particularly fitted for kinetic studies (the adsorption of protein and its elimination of hormone not bound to the receptor being very rapid) and allows for working with samples of large volume and low protein content (because protein is quantitatively and rapidly adsorbed even from very dilute solutions). The equilibrium association constant of the receptor can be calculated from data of experiments carried out by the hydroxylapatite-column method by the use of a correction factor, or directly, if the extract is diluted to contain less than 0.25 mg protein/ml. © 1979.

关键词： R uterine estradiol receptor X other estradiol-binding macromolecules than R in a crude uterine extract E estradiol [ 3 H]-E tritiated estradiol E t total E in a system E f free E E bR E bound to R e bx E bound to X E b E bound to macromolecules under equilibrium conditions (E b = E bR + E bx ) E u E unbound to R (E u = E f + E bx ) E bnonspec E bound “non specifically” to the HTP column HTP hydroxylapatite EDIAL equilibrium dialysis α the value of the ratio E bX /E f , when the latter is constant under equilibrium conditions K A equilibrium constant of association

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Photoreduction Reactions of flavin Coenzymes - A Laser flash Photolysis Study

Photobiochemistry and Photobiophysics

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Photobiochemistry and Photobiophysics 1979年第1期1卷 63-70页

作者： Paul f. Heelis Glynn O. Phillips The North East Wales Institute of Higher Education Kelsterton College Connah's Quay Clwyd Wales

The primary reactions of the neutral and protonated riboflavin triplet states with phenylacetic acid, indole-acetic acid and several similar derivatives have been examined by laser flash photolysis. The results indicate that one-electron transfer to the flavin triplet state(s) is the major reaction of all the substrates studied, two electron processes (group transfer) are not significant. The observed difference in reactivity of each substrate with the neutral and protonated triplet states is discussed in terms of the pH dependence of photoreduction, previously reported in the literature.

关键词： f, fH 2, HfR oxidised flavin 1,5-dihydroflavin and flavin adducts respectively 3 f and 3 fH + the neutral and protonated flavin triplet states respectively fH - f - the neutral and anionic flavin semiquinone radicals respectively RH R- RH + a substrate its neutral and cationic radicals respectively

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SOIL MARK STUDIES NEAR WINCHESTER, HAMPSHIRE

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JOURNAL Of ARCHAEOLOGICAL SCIENCE 1979年第1期6卷 93-100页

作者： TAYLOR, TP INST ARCHAEOL 31 34 GORDON SQLONDON WC1ENGLAND

The fate of artifacts incorporated into the ploughsoil as a result of plough damage is considered in terms of two soil mark sites near Winchester, Hampshire. Ploughsoil and subsoil analyses indicate that dispersal of soil material, as a result of ploughing, is limited at the present time and under mould board ploughing techniques. The origin of soil marks underlain only by bedrock is also considered and evidence indicates that such soil marks may appear as groundsurface expressions of ditches which have been removed by plough damage where dispersal of ploughsoil is minimal. Such soil marks may be only short lived if plough depth continues to increase.

关键词： Winchester Particle size analysis Dispersal f C ratio Permeability Plough damage Sand median Soil mark

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LMfBR pool plant: 1000 MWe. Volume 2. NSSS plant description. final report

LMFBR pool plant: 1000 MWe. Volume 2. NSSS plant description...

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作者： McDonald, J. S. Rockwell International Corp. Canoga Park CA (USA). Energy Systems Group

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REGULATION Of PROTEIN-SYNTHESIS IN RABBIT RETICULOCYTE LYSATES BY HEME-REGULATED PROTEIN-KINASE - INHIBITION Of INTERACTION Of MET-TRANSfER-RNA-f(MET) BINDING fACTOR WITH ANOTHER INITIATION-fACTOR IN fORMATION Of MET-TRANSfER-RNA-f(MET).40S RIBOSOMAL-SUBUNIT COMPLEXES

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PROCEEDINGS Of THE NATIONAL ACADEMY Of SCIENCES Of THE UNITED STATES Of AMERICA 1978年第2期75卷 745-749页

作者： RANU, RS LONDON, IM DAS, A DASGUPTA, A MAJUMDAR, A RALSTON, R ROY, R GUPTA, NK HARVARD UNIV MIT DIV HLTH SCI & TECHNOL CAMBRIDGE MA 02139 USA HARVARD UNIV SCH MED DEPT MED BOSTON MA 02115 USA UNIV NEBRASKA DEPT CHEM LINCOLN NE 68588 USA

Protein synthesis in reticulocytes and their lysates is regulated by heme. In heme deficiency a heme-regulated translational inhibitor (HRI) that blocks initiation of polypeptide chains is activated. HRI is a protein kinase (ATP: protein phosphotransferase, EC 2.7.1.37) that specifically phosphorylates the 38,000 dalton subunit of the Met-tRNAfMet binding factor (If), which forms a ternary complex with Met-tRNAfMet and GTP, a finding that suggests that the inhibition by HRI involves the phosphorylation of If. The effect of HRI in the partial reactions of protein chain initiation in which the If-promoted binding of Met-tRNAfMet to 40S ribosomal subunits is enhanced by another initiation factor [ternary complex dissociation factor (TDf)] and AUG was investigated HRI at very low concentrations markedly inhibits the bindings of Met-tRNAfMet to 40S subunits. The inhibitory effect of HRI requires ATP. Under these conditions HRI phosphorylates only the 38,000 dalton subunit of If The TDf preparations not only promote the binding of the ternary complex to 40S subunits but also promote the dissociation of the ternary complex in the presence of 5 mM Mg2+ at ***. C. The preincubation of purified If alone with low concentrations of HRI and ATP does not significantly affect its capacity to form the ternary complex;however, the TDf-promoted dissociation of the ternary complex is inhibited. The nonhydrolyzable analog adenosine 5''[.beta..gamma.-imido]triphosphate does not substitute for ATP. Phosphorylation apparently causes a conformational modification in If, which results in inhibition of the interaction between the ternary complex and TDf that is required for the binding of the ternary complex to 40S subunits.

关键词： phosphorylation of Met-tRNA f Met binding factor translational regulation inhibition of Met-tRNA f Met binding 40S ribosomal subunits protein—protein interaction

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