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INfINITE-VARIATE WIDE-SENSE MARKOV-PROCESSES AND fUNCTIONAL-ANALYSIS fOR BOUNDED OPERATOR-fORMING VECTORS

JOURNAL Of MULTIVARIATE ANALYSIS 1978年第2期8卷 295-316页

作者： ROSENBERG, M Rockaway Beach New York USA

Let p , q be arbitrary parameter sets, and let H be a Hilbert space. We say that x = ( x i ) iϵq , x i ϵ H , is a bounded operator-forming vector (ϵ H f q ) if the Gram matrix 〈 x , x 〉 = [( x i , x j )] iϵq , jϵq is the matrix of a bounded (necessarily ≥ 0) operator on l q 2 , the Hilbert space of square-summable complex-valued functions on q . Let A be p × q , i.e., let A be a linear operator from l q 2 to l p 2 . Then exists a linear operator Ǎ from (the Banach space) H f q to H f p on D (A) = {x:x ϵ H f q , A〈x, x〉 1 2 is p × q bounded on l q 2 } such that y = Ǎx satisfies y j ϵ σ ( x ) = {space spanned by the x i }, 〈 y , x 〉 = A 〈 x , x 〉 and 〈y, y〉 = A〈x, x〉 1 2 (A〈x, x〉 1 2 ) ∗ . This is a generalization of our earlier [ J. Multivariate Anal. 4 (1974), 166–209; 6 (1976), 538–571] results for the case of a spectral measure concentrated on one point. We apply these tools to investigate q-variate wide-sense Markov processes.

关键词： 60J99 46C10 46B99 47A60 60G10 47D05 60J05 60J25 28A45 60G25 H f q p × qL 2. x f bounded operator-forming vectors p × q stochastic integral p × q spectral integral p × q correlation operator p × q transition operator q -variate wide-sense Markov process q -variate stationary process semigroup contraction operator semigroup q × q positive-definite function associated discrete parameter process q × q spectral measure nondeterministic process Wold decomposition

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USE Of TERT-STATISTIC IN PRELIMINARY TESTING PROCEDURES fOR BEHRENS-fISHER PROBLEM

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JOURNAL Of STATISTICAL PLANNING AND INfERENCE 1978年第1期2卷 15-25页

作者： WEHRHAHN, K OGAWA, J UNIV CALGARY DEPT MATH & STATCALGARY T2N 1N4ALBERTACANADA

Preliminary testing procedures for the two means problem traditionally employ the pooled variance t -statistic. In this paper we show that bias of the t -statistic under conditions of heterogeneity of variance may be increased if use of the t -statistic is conditional on an affirmative f -test. for this reason we conclude that use of the t -statistic in preliminary testing procedures is inappropriate.

关键词： 62f05 Preliminary testing procedures t -statistic, Heterogeneity of variances Behrens-fisher problem Preliminary f -test

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IMMUNOGEN STRUCTURE AND CELL SELECTION .2. PREPARATION Of A MINIMAL SOLID-PHASE IMMUNOGEN fOR USE IN MINI-CULTURES

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JOURNAL Of IMMUNOLOGICAL METHODS 1978年第3-4期23卷 327-334页

作者： TRUMP, GN UNIV SO CALIF SCH DENT DEPT MICROBIOL & IMMUNOL LOS ANGELES CA 90007 USA

A solid-phase haptenic immunogen, DNP-PAB [dinitrophenyl-polyacrylamide beads], was prepared through the reaction of the hydrazide derivative of PB with dinitrobenzenesulfonic acid. A new method was presented for quantifying the number of haptenic groups. DNP-PAB is very immunogenic for dissociated mouse spleen cells in culture, and the anti-hapten response is highly specific.

关键词： B-Cell: Bursal-equivalent small lymphocyte B 6 D 2 f 1 Mice: The f 1 hybrid C57B1/6 × DBA mice DNBS: Dinitrobenzenesulphonic acid DNP: 2,4-dinitrophenol DNP-O-PAB: Dinitrophenyl-ornithine-polyacrylamide beads DNP-PAB: Dinitrophenyl-polyacrylamide beads Hz: Hydrazide PAB: Polyacrylamide beads PfC: Plaque forming cells SRBC Sheep red blood cells SucA: Succinic anhydride TNP: 2,4,6-trinitrophenol

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MASTOPHORUS-MURIS (NEMATODA SPIRURINA) - ULTRASTRUCTURE Of SOMATIC MUSCLE DEVELOPMENT

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INTERNATIONAL JOURNAL fOR PARASITOLOGY 1978年第5期8卷 405-414页

作者： HAMADA, GS WERTHEIM, G Laboratory of Helminthology The Hebrew University-Hadassah Medical School Jerusalem Israel

The ultrastructure of the somatic muscle cells of the adult and 6 developmental stages of M. muris were studied. In all stages the cells consisted of a contractile region containing myofibrils separated by dense bands and a noncontractile region with nuclei, mitochondria, glycogen, lipid droplets and vesicles. Two sizes of myofilaments were present. The dense band contained T[transverse]-tubules and sarcoplasmic reticulum, and, in more advanced stages, support filaments, glycogen and dense bodies. The contractile region of the adult muscle cell consisted of several hundred irregularly shaped myofibrils arranged in a random pattern. This pattern of myofibrils was defined as irregular-coelomyarian. The 3rd stage larva had a shallow-coelomyarian myofibril configuration, which changed to coelomyarian in the late 3rd stage through the addition of new myofibrils at the apical contractile border. In the 4th stage larvae, the subdivision of existing myofibrils changed the pattern to irregular-coelomyarian.

关键词： Mastophorus muris muscle development nematode somatic muscle ultrastructure c contractile region d dense band db membrane bound dense body f fibrillar dense bodies g glycogen h hypodermis m myofibril mT thick myofilaments mt thin myofilaments n noncontractile region sf support filaments sr sarcoplasma reticulum t T tubule

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INTERACTIONS BETWEEN PHOTOSYSTEM-II COMPONENTS IN CHLOROPLAST MEMBRANES - CORRELATION BETWEEN EXISTENCE Of A LOW POTENTIAL SPECIES Of CYTOCHROME-B-559 AND LOW CHLOROPHYLL fLUORESCENCE IN INHIBITED AND DEVELOPING CHLOROPLASTS

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第2期503卷 274-286页

作者： HORTON, P CROZE, E SMUTZER, G SUNY BUFFALO DIV CELLULAR & MOLEC BIOL BUFFALO NY 14214 USA

Chloroplasts [pea] inhibited by incubation with h-droxylamine in the light exhibit a low fluorescence yield upon illumination in the presence of dithionite sufficient to completely reduce the primary acceptor, Q. In the absence of Mg ions, the fluorescence yield is the same as in control chloroplasts, suggesting that the reason for the low yield is a defect in the mechanism by which Mg2+ enhances the fluorescence. These chloroplasts were previously shown to contain only low potential (Em7.8 = +80 mV) cytochrome b-559. In Photosystem II particles, in heat-treated chloroplasts and in trypsin-digested chloroplasts, high potential cytochrome b-559 is absent and the variable fluorescence yield is again low. Peas grown under intermittent light contain only 1/5 of the content of high potential cytochrome b-559 seen in fully greened plants, yet show high rates of water to methyl viologen electron transport. Aquisition of the high potential cytochrome b-559 accompanies synthesis of chlorophyll b, the onset of Mg-stimulated fluorescence and an increased variable yield of fluorescence. A similar correlation was seen during greening of dark-grown barley. Apparently the high potential state of cytochrome b-559 is due to the same membrane properties which allow cation enhanced variable fluorescence, so that the presence of low potential cytochrome b-559 is accompanied by a decrease in variable fluorescence yield.

关键词： f 0 initial fluorescence yield f v variable fluorescence yield NH 2 OH light chloroplasts chloroplasts inhibited with hydroxylamine plus light [2] NH 2 OH dark chloroplasts chloroplasts inhibited with hydroxylamine in darkness [2] E m midpoint oxidation reduction potential HEPES N-2- hydroxy-ethylpiperazine -N′-2- ethanesulfonic acid

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INTERACTIONS Of ACETYLCHOLINE-RECEPTOR AND ACETYLCHOLINESTERASE WITH LIPID MONOLAYERS

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第2期506卷 161-172页

作者： WIEDMER, T BRODBECK, U ZAHLER, P fULPIUS, BW UNIV BERN INST MED CHEM CH-3000 BERN 9 SWITZERLAND INST BIOCHEM FREIESTR 3 CH-3000 BERN 9 SWITZERLAND DEPT BIOCHIM SCI 2 CH-1211 GENEVE 4 SWITZERLAND

The interaction of acetylcholine receptor [from the electric organ of Torpedo marmorata] and acetylcholinesterase [from the electric organ of Electrophorus electricus] with lipid monolayers was followed by measuring changes in surface pressure. When injected into the subphase of a lipid monolayer, the proteins caused increases in surface pressure from 5 to 10 dynes/cm, indicating a penetration of protein into the monolayer. At pH values below the isoelectric point of the proteins the incorporation was improved. The same was observed when Ca2+ (2 mM) was added. The presence of the enzyme in the mixed film was demonstrated by using diiso[3H]propyl fluorophosphate-labeled acetylcholinesterase and by measuring enzyme activity. Acetylcholine receptor was demonstrated in the mixed film by using a complex made of the receptor and .alpha.-[3H]neurotoxin.

关键词： BBOT 2,5-bis-[5′- tert -butylbenzoxazolyl-(2′)]thiophene iPr 2 P- f diisopropyl fluorophosphate Nbs 2 5,5′-dithiobis(2-nitro-benzoic acid) O-CH 3 -phosphatidylcholine 1,2- dipalmitoyl -3-P- methoxy-phosphatidylcholine

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A NOVEL METHOD fOR DETERMINING EQUILIBRIUM-CONSTANTS - CTP - PHOSPHORYLCHOLINE CYTIDYLTRANSfERASE

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第2期526卷 440-449页

作者： INfANTE, JP KINSELLA, JE CORNELL UNIV COLL AGR & LIFE SCI DEPT FOOD SCI ITHACA NY 14853 USA

A novel method for the determination of equilibrium constants for reversible reactions is described. The method is based on the measurement of initial velocities of isotope transfer for a given substrate-product pair of the forward and reverse reactions as a function of the mass action of reactants. The reciprocal values of these initial velocities are plotted against the mass action ratios of reactants. The observed Keq is the abscissa of the intersection point of these reciprocal plots, i.e., the mass action ratio at which the initial velocities of isotope transfer for both the forward and reverse reaction are identical, i.e., when isotope exchange is occurring. In this manner, an observed Keq of 0.2 was obtained from CTP:phosphorylcholine cytidyltransferase (CTP:cholinephosphate cytidyltransferase, EC 2.7.7.15) at ***. C and pH 7.5 under physiological conditions 1.0 mM free Mg2+ and 0.15 M salt concentration. A comparison of this value with the in vivo mass action of reactants calculated from published data indicates that this reaction is rate-limiting in the rat liver.

关键词： CDP-choline, cytidine 5′-diphosphocholine [Mg 2+ ] f , free magnesium concentration cytidyltransferase, CTP:cholinephosphate cytidyltransferase (EC 2.7.7.15) pyrophosphorylase CDP-choline pyrophosphorylase (EC 3.6.1.-) [R] t , total concentration of reactant R E, enzyme Γ, mass action ratio of reactants υ∗, initial velocity of isotope transfer, V f and V r , maximum forward and reverse velocities, respectively f , net flux of velocity through a metabolic step υ f and υ r , forward and reverse initial velocities of an enzymatic reaction

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REGULATION Of PROTEIN-SYNTHESIS - ACTIVATION BY DOUBLE-STRANDED-RNA Of A PROTEIN-KINASE THAT PHOSPHORYLATES EUKARYOTIC INITIATION fACTOR-2

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PROCEEDINGS Of THE NATIONAL ACADEMY Of SCIENCES Of THE UNITED STATES Of AMERICA 1978年第3期75卷 1121-1125页

作者： LEVIN, D LONDON, IM HARVARD UNIV MIT DIV HLTH SCI & TECHNOL CAMBRIDGE MA 02139 USA HARVARD UNIV DEPT MED CAMBRIDGE MA 02139 USA

Incubation of reticulocyte lysates [rabbit] or isolated crude ribosomes with low levels of double-stranded RNA (0.1-10 ng/ml) induces the formation of an inhibitor of protein synthesis initiation similar to that observed in heme deficiency. The inhibitor is associated with a cyclic[c]AMP-independent protein kinase activity (ATP:protein phosphotransferase, EC 2.7.1.37) that phosphorylates the small polypeptide (38,000 daltons) of the eukaryotic initiation factor eIf-2. Activation of the inhibitor requires ATP in addition to double-stranded RNA and is accompanied by the phosphorylation of a 67,000 dalton polypeptide of unknown function. The inhibitor remains associated with the ribosomes during high-speed sedimentation. Once formed, the ribosome-associated inhibitor phosphorylates eIf-2 and inhibits protein synthesis in the absence of double-stranded RNA. Inhibition is prevented by exogenous eIf-2. The bound inhibitor can be solubilized by extraction with 0.5 M KCl. The soluble inhibitor preparation retains the ability to phosphorylate the small polypeptide of eIf-2 and to inhibit protein synthesis. Untreated crude ribosomes also contain cAMP-independent protein kinase activities that phosphorylate the middle polypeptide (49,000 daltons) of eIf-2 and several polypeptide subunits of eIf-3 (160,000, 125,000 and 65,000 daltons);these kinase activities are not affected by double-stranded RNA and do not inhibit protein synthesis.

关键词： initiation in reticulocyte lysates translational control Met-tRNA f Met

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ENHANCEMENT Of ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSIS Of SPECIfIC PARA-NITROANILIDE SUBSTRATES BY 4-PHENYLBUTYLAMINE DERIVATIVE Of HEN EGG-WHITE LYSOZYME

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第1期527卷 42-55页

作者： SHECHTER, Y GERTLER, A HEBREW UNIV JERUSALEM FAC AGR DEPT AGR BIOCHEM JERUSALEM 91000 ISRAEL WEIZMANN INST SCI DEPT ORGAN CHEM REHOVOT ISRAEL

Modification of hen egg-white lysozyme by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in presence of 4-phenylbutylamine yielded derivatives, which contained 0.6-0.7 modified residues and retained about 60% of the original activity. Kinetic studies revealed that the modified-lysozyme increases approx. 20-fold the kcat of hydrolysis of SucGly2Phe-4-nitroanilide by .alpha.-chymotrypsin, without changing the Km. The apparent dissociation constant of phenylbutylamine-modified lysozyme .cntdot. chymotrypsin complex was 0.03 mM and independent of substrate concentration. The accelerating effect of the modified lysozyme was also observed with other p-nitroanilide substrates of .alpha.-chymotrypsin. The hydrolysis of other substrates, acylation by active site titrant or inhibition by irreversible or competitive inhibitors were unaffected. The enhancing effect of the modified lysozyme seems to be very specific since other chymotrypsin-like enzymes, or serine proteinases except .delta.-chymotrypsin, were not influenced and phenylbutylamine derivatives of .alpha.-lactalbumin or ribonuclease were lacking any enhancing effect. Smaller, but significant enhancing effect was found also in lysozyme substituted by benzylamine, .beta.-phenylethylamine and tryptamine and in inactive derivatives of lysozyme substituted by phenylbutylamine. Competitive inhibitors of lysozyme such as N-acetyl-D-glucose amine oligomers, (GlcNAc)2 and (GlcNAc)3 abolished partially the accelerating effect of phenylbutylamine-modified lysozyme, indicating that the substituted group is located in the vicinity of the binding site.

关键词： TosPheCk L-(1-tosylamido-2-phenyl)-ethyl chloromethyl ketone Ac N -acetyl Bz N -benzoyl Suc N -succinyl PhCH 2 SO 2 f phenylmethanesulfonyl fluoride, OME, methyl ester OEt ethyl ester

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LIMITED COOPERATIVITY IN COUPLING BETWEEN ELECTRON fLOW AND PHOTOSYNTHETIC ATP SYNTHESIS - COMPARATIVE-STUDY IN CHROMATOPHORES PHOSPHORYLATING AT VERY DIffERENT RATES

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fEBS LETTERS 1978年第2期87卷 323-328页

作者： CASADIO, R BACCARINIMELANDRI, A MELANDRI, BA UNIV BOLOGNA INST BOTI-40126 BOLOGNAITALY

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