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EUGLENA-GRACILIS N10-fORMYL-H4fOLATE - METHIONYL-TRANSfER RNA-f TRANSfORMYLASE - SITE Of SYNTHESIS AND REGULATION Of ENZYME-SYNTHESIS

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PLANT SCIENCE LETTERS 1978年第1期12卷 1-7页

作者： GAMBINI, A Centro di studio del CNR per la Biologia Cellulare e Molecolare delle Piante. Istituto di Scienze Botaniche dell 'Universita degli Studi di Milano. via G. Colombo 60 Milano Italy

The site of biosynthesis of transformylase, an enzyme of plastid and mitochondrial protein synthesizing system, was studied in autotrophic and heterotrophic E. gracilis cells. In light-grown cells and in resting dark-grown cells cultivated in light, chloramphenicol (CAP) didn''t modify the level of transformylase activity. The enzyme from dark-grown cells was unaffected by the addition of ethidium bromide to the medium, whereas transformylase accumulation was stimulated by addition of CAP. Apparently both plastidial and mitochondrial transformylase are synthesized on cytoplasmic ribosomes. A regulation mechanism for the etioplast transformylase is proposed.

关键词： CAP cloroamphenicol met-tRNA f formylatable methionyl-t-RNA

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HYPOTENSIVE EffECT INDUCED BY A CYCLIC DOPAMINE ANALOG, TRANS-4-METHYL-7,8-DIHYDROXY-1,2,3,4,4A,5,6,10B-OCTAHYDROBENZO[f]QUINOLINE

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JOURNAL Of PHARMACEUTICAL SCIENCES 1978年第11期67卷 1639-1641页

作者： SHARABI, fM LONG, JP CANNON, JG UNIV IOWA COLL MED DEPT PHARMACOL IOWA CITY IA 52242 USA UNIV IOWA COLL PHARM DIV MED CHEM & NAT PROD IOWA CITY IA 52242 USA

The antihypertensive effects of trans-4-methyl-7,8-dihydroxy-1,2,3,4,4a,5,6,10b-octahydrobenzo[f]quinoline, a dopamine agonist, were investigated in dogs and spontaneous hypertensive rats. I.v. administration of low doses of this cyclic analog of dopamine (0.25-1.00 .mu.g/kg) consistently reduced blood pressure and heart rate, concurrently recorded in the dog. This effect was antagonized by haloperidol, a specific dopamine antagonist. The dopamine analog also reduced systolic blood pressure of spontaneous hypertensive rats. The possibility that the decrease in blood pressure and heart rate elicited by the dopamine analog is attributable to an effect on specific dopaminergic receptors is confirmed.

关键词： trans -4-Methyl-7,8-dihydroxy-1,2,3,4,4a,5,6,1 Ob-octa- hydrobenzo[ f ]quinoline—hypotensive activity evaluated in dogs and spontaneous hypertensive rats Benzo[ f ]quinoline, substituted— hypotensive activity evaluated in dogs and spontaneous hypertensive rats Hypotensive activity—substituted benzo[ f ]quinoline evaluated in dogs and spontaneous hypertensive rats Dopamine analogs, cyclic—hypotensive activity evaluated in dogs and spontaneous hypertensive rats

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SPECIfIC LABELING Of (CA2+ + MG2+)-ATPASE Of ESCHERICHIA-COLI WITH 8-AZIDO-ATP AND 4-CHLORO-7-NITROBENZOfURAZAN

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第2期502卷 345-353页

作者： VERHEIJEN, JH POSTMA, PW VANDAM, K UNIV AMSTERDAM BCP JANSEN INST BIOCHEM LAB AMSTERDAM 1004 NETHERLANDS

8-Azido-ATP is a substrate for E. coli (Ca2+ + Mg2+)-ATPase (E. coli f1). Illumination of E. coli f1 in the presence of 8-azido-ATP causes inhibition of ATPase activity. The presence of ATP during illumination prevents inhibition. 8-Azido-ATP and 4-chloro-7-nitrobenzofurazan (NbfCl) bind predominantly to the .alpha. subunit of the enzyme, but also significantly to the .beta. subunit. The .alpha. subunit of E. coli f1 had some properties that in other f11-ATPases are associated with the .beta. subunit.

关键词： f 1 ATPase from various energy-transducing membranes NbfCl 4-chloro-7-nitrobenzofurazan

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INTERACTION Of PEANUT AGGLUTININ WITH HUMAN LYMPHOCYTES - BINDING PROPERTIES AND TOPOLOGY Of RECEPTOR-SITE

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ZEITSCHRIfT fUR IMMUNITATSfORSCHUNG-IMMUNOBIOLOGY 1978年第5期154卷 451-462页

作者： NEWMAN, RA UHLENBRUCK, G SCHUMACHER, K MIL, AV KARDUCK, D UNIV COLOGNE MED KLIN IMMUNOBIOL ABT D-5000 COLOGNE 41 FED REP GER

The relationship between the mitogenic lectin PNA [peanut agglutinin] and other mitogenic and non-mitogenic lectins was investigated. PNA labeled with 125I bound equally well to T [thymus derived] and B [bone marrow derived] lymphocytes, after neuraminidase treatment, with 3.88 .times. 106 and 3.73 .times. 106 binding sites, respectively. Only the T cell fraction was stimulated and only after neuraminidase treatment. Preincubation of neuraminidase-treated cells with non-mitogenic lectins and antiserum, which appeared to bind to the same receptor as PNA, enhanced the latter''s stimulatory properties. Capping and co-capping techniques were used to examine the topology of lectin receptors on the lymphocyte surface. The receptor glycoprotein for the mitogenic PNA lectin was distinct from that possessing the Con A [concanavalin A] and PHA [phytohemagglutinin] receptors, and that possessing the receptor for the non-mitogenic lectin from Helix pomatia.

关键词： PNA Peanut Agglutinin ( Arachis hypogaea ) Con A Concanavalin A ( Canavalia ensiformis ) PHA Phytohaemagglutinin ( Phaseolus vulgaris ) Ab-L Mushroom lectin ( Agaricus bisporus ) HP Helix pomatia lectin SBA Soya bean agglutinin ( Soya hispida ) f fluorescein labelled TMB Tetramethyl-rhodamine labelled.

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PURIfICATION AND PROPERTIES Of A NUCLEOSIDE DIPHOSPHOSUGAR - NAD+ 2-HEXOSYL OXIDOREDUCTASE

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第1期523卷 9-18页

作者： JOHNSON, MT GANDER, JE UNIV MINNESOTA COLL BIOL SCI DEPT BIOCHEM ST PAUL MN 55108 USA

Penicillium charlesii contains a nucleoside diphosphosugar:NAD 2-hexosyl oxidoreductase that oxidizes UDPgalactose (UDP-Galp), ADPribose [1,2] and UDPglucose (UDP-Glc). Dithiothreitol, NAD and 0.25 M NaCl but not nucleoside diphosphates stabilize the enzyme activity. The enzyme was purified and separated on polyacrylamide disc gels by electrophoresis into 1 major and 8 minor bands of protein. Oxidoreductase activity was located in the major and 3 of the minor bands of protein. Each of these proteins catalyze the oxidation of UDPGalp, ADPribose and UDP-Glc.

关键词： UDP-Gal p uridine-5-(α- d -galactopyranosyl pyrophosphate) UDP-Gal f uridine-5′-(α- d -galactofuranosyl pyrophosphate) UDP-Glc uridine-5′-(α- d -glucopyranosyl pyrophosphate) ADPribose adenosine-5′-(ribose-5-pyrophosphate) ADP-3-keto-ribose adenosine -5′-( d -glycero- d -glycero- pentosulose -5- pyrophosphate ) UDP-2-keto-Gal p uridine -5′-( d -arabino-2- hexosulopyranosyl pyrophosphate )

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INfINITE-VARIATE WIDE-SENSE MARKOV-PROCESSES AND fUNCTIONAL-ANALYSIS fOR BOUNDED OPERATOR-fORMING VECTORS

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JOURNAL Of MULTIVARIATE ANALYSIS 1978年第2期8卷 295-316页

作者： ROSENBERG, M Rockaway Beach New York USA

Let p , q be arbitrary parameter sets, and let H be a Hilbert space. We say that x = ( x i ) iϵq , x i ϵ H , is a bounded operator-forming vector (ϵ H f q ) if the Gram matrix 〈 x , x 〉 = [( x i , x j )] iϵq , jϵq is the matrix of a bounded (necessarily ≥ 0) operator on l q 2 , the Hilbert space of square-summable complex-valued functions on q . Let A be p × q , i.e., let A be a linear operator from l q 2 to l p 2 . Then exists a linear operator Ǎ from (the Banach space) H f q to H f p on D (A) = {x:x ϵ H f q , A〈x, x〉 1 2 is p × q bounded on l q 2 } such that y = Ǎx satisfies y j ϵ σ ( x ) = {space spanned by the x i }, 〈 y , x 〉 = A 〈 x , x 〉 and 〈y, y〉 = A〈x, x〉 1 2 (A〈x, x〉 1 2 ) ∗ . This is a generalization of our earlier [ J. Multivariate Anal. 4 (1974), 166–209; 6 (1976), 538–571] results for the case of a spectral measure concentrated on one point. We apply these tools to investigate q-variate wide-sense Markov processes.

关键词： 60J99 46C10 46B99 47A60 60G10 47D05 60J05 60J25 28A45 60G25 H f q p × qL 2. x f bounded operator-forming vectors p × q stochastic integral p × q spectral integral p × q correlation operator p × q transition operator q -variate wide-sense Markov process q -variate stationary process semigroup contraction operator semigroup q × q positive-definite function associated discrete parameter process q × q spectral measure nondeterministic process Wold decomposition

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USE Of TERT-STATISTIC IN PRELIMINARY TESTING PROCEDURES fOR BEHRENS-fISHER PROBLEM

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JOURNAL Of STATISTICAL PLANNING AND INfERENCE 1978年第1期2卷 15-25页

作者： WEHRHAHN, K OGAWA, J UNIV CALGARY DEPT MATH & STATCALGARY T2N 1N4ALBERTACANADA

Preliminary testing procedures for the two means problem traditionally employ the pooled variance t -statistic. In this paper we show that bias of the t -statistic under conditions of heterogeneity of variance may be increased if use of the t -statistic is conditional on an affirmative f -test. for this reason we conclude that use of the t -statistic in preliminary testing procedures is inappropriate.

关键词： 62f05 Preliminary testing procedures t -statistic, Heterogeneity of variances Behrens-fisher problem Preliminary f -test

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IMMUNOGEN STRUCTURE AND CELL SELECTION .2. PREPARATION Of A MINIMAL SOLID-PHASE IMMUNOGEN fOR USE IN MINI-CULTURES

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JOURNAL Of IMMUNOLOGICAL METHODS 1978年第3-4期23卷 327-334页

作者： TRUMP, GN UNIV SO CALIF SCH DENT DEPT MICROBIOL & IMMUNOL LOS ANGELES CA 90007 USA

A solid-phase haptenic immunogen, DNP-PAB [dinitrophenyl-polyacrylamide beads], was prepared through the reaction of the hydrazide derivative of PB with dinitrobenzenesulfonic acid. A new method was presented for quantifying the number of haptenic groups. DNP-PAB is very immunogenic for dissociated mouse spleen cells in culture, and the anti-hapten response is highly specific.

关键词： B-Cell: Bursal-equivalent small lymphocyte B 6 D 2 f 1 Mice: The f 1 hybrid C57B1/6 × DBA mice DNBS: Dinitrobenzenesulphonic acid DNP: 2,4-dinitrophenol DNP-O-PAB: Dinitrophenyl-ornithine-polyacrylamide beads DNP-PAB: Dinitrophenyl-polyacrylamide beads Hz: Hydrazide PAB: Polyacrylamide beads PfC: Plaque forming cells SRBC Sheep red blood cells SucA: Succinic anhydride TNP: 2,4,6-trinitrophenol

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MASTOPHORUS-MURIS (NEMATODA SPIRURINA) - ULTRASTRUCTURE Of SOMATIC MUSCLE DEVELOPMENT

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INTERNATIONAL JOURNAL fOR PARASITOLOGY 1978年第5期8卷 405-414页

作者： HAMADA, GS WERTHEIM, G Laboratory of Helminthology The Hebrew University-Hadassah Medical School Jerusalem Israel

The ultrastructure of the somatic muscle cells of the adult and 6 developmental stages of M. muris were studied. In all stages the cells consisted of a contractile region containing myofibrils separated by dense bands and a noncontractile region with nuclei, mitochondria, glycogen, lipid droplets and vesicles. Two sizes of myofilaments were present. The dense band contained T[transverse]-tubules and sarcoplasmic reticulum, and, in more advanced stages, support filaments, glycogen and dense bodies. The contractile region of the adult muscle cell consisted of several hundred irregularly shaped myofibrils arranged in a random pattern. This pattern of myofibrils was defined as irregular-coelomyarian. The 3rd stage larva had a shallow-coelomyarian myofibril configuration, which changed to coelomyarian in the late 3rd stage through the addition of new myofibrils at the apical contractile border. In the 4th stage larvae, the subdivision of existing myofibrils changed the pattern to irregular-coelomyarian.

关键词： Mastophorus muris muscle development nematode somatic muscle ultrastructure c contractile region d dense band db membrane bound dense body f fibrillar dense bodies g glycogen h hypodermis m myofibril mT thick myofilaments mt thin myofilaments n noncontractile region sf support filaments sr sarcoplasma reticulum t T tubule

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INTERACTIONS BETWEEN PHOTOSYSTEM-II COMPONENTS IN CHLOROPLAST MEMBRANES - CORRELATION BETWEEN EXISTENCE Of A LOW POTENTIAL SPECIES Of CYTOCHROME-B-559 AND LOW CHLOROPHYLL fLUORESCENCE IN INHIBITED AND DEVELOPING CHLOROPLASTS

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BIOCHIMICA ET BIOPHYSICA ACTA 1978年第2期503卷 274-286页

作者： HORTON, P CROZE, E SMUTZER, G SUNY BUFFALO DIV CELLULAR & MOLEC BIOL BUFFALO NY 14214 USA

Chloroplasts [pea] inhibited by incubation with h-droxylamine in the light exhibit a low fluorescence yield upon illumination in the presence of dithionite sufficient to completely reduce the primary acceptor, Q. In the absence of Mg ions, the fluorescence yield is the same as in control chloroplasts, suggesting that the reason for the low yield is a defect in the mechanism by which Mg2+ enhances the fluorescence. These chloroplasts were previously shown to contain only low potential (Em7.8 = +80 mV) cytochrome b-559. In Photosystem II particles, in heat-treated chloroplasts and in trypsin-digested chloroplasts, high potential cytochrome b-559 is absent and the variable fluorescence yield is again low. Peas grown under intermittent light contain only 1/5 of the content of high potential cytochrome b-559 seen in fully greened plants, yet show high rates of water to methyl viologen electron transport. Aquisition of the high potential cytochrome b-559 accompanies synthesis of chlorophyll b, the onset of Mg-stimulated fluorescence and an increased variable yield of fluorescence. A similar correlation was seen during greening of dark-grown barley. Apparently the high potential state of cytochrome b-559 is due to the same membrane properties which allow cation enhanced variable fluorescence, so that the presence of low potential cytochrome b-559 is accompanied by a decrease in variable fluorescence yield.

关键词： f 0 initial fluorescence yield f v variable fluorescence yield NH 2 OH light chloroplasts chloroplasts inhibited with hydroxylamine plus light [2] NH 2 OH dark chloroplasts chloroplasts inhibited with hydroxylamine in darkness [2] E m midpoint oxidation reduction potential HEPES N-2- hydroxy-ethylpiperazine -N′-2- ethanesulfonic acid

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