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HORMONE-RECEPTOR INTERACTIONS - ADRENOCORTICOTROPHIN-(7-24)-OCTADECAPEPTIDE STIMULATES ADIPOCYTE MEMBRANE ADENYLATE-CYCLASE WITHOUT CAUSING LIPOLYSIS IN fAT-CELLS

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fEBS LETTERS 1976年第2期66卷 246-249页

作者： LANG, U fAUCHERE, JL PELICAN, GM KARLAGANIS, G SCHWYZER, R ETH INST MOLEK BIOL & BIOPHYSCH-8093 ZURICHSWITZERLAND

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The interaction of α-1-antitrypsin with soluble and sepharose-bound elastase

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BBA - Protein Structure 1976年第2期453卷 344-356页

作者： Lo, Theresa N. Cohen, Allen B. James, Harold L. Department of Medicine San Francisco General Hospital the Pulmonary Specialized Center of Research and the Cardiovascular Research Institute University of California San Francisco Calif. U.S.A. Department of Medicine Temple University Health Sciences Center Philadelphia PA United States

The products resulting from the interaction of α-1-antitrypsin with elastase were examined with polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate, and by affinity chromatography. five products of the reaction can be identified by polyacrylamide disc gel electrophoresis. Two products are complexes between α-1-antitrypsin and elastase (73 800 and 58 300 daltons). Two additional products are identical to fragments of α-1-antitrypsin which can be washed from a column of Sepharose-bound elastase immediately after α-1-antitrypsin is applied to the column. The larger component is about 50 000 daltons, reacts with antiserum to α-1-antitrypsin, and does not inhibit enzymes. Together, these two products have an amino acid analysis similar to α-1-antitrypsin. These two fragments are probably hydrolytic products of the interaction of elastase with α-1-antitrypsin which is biologically inactive. The fifth product is probably a fragment of α-1-antitrypsin missing from the low molecular weight complex. The components of the complexes can be separated from each other by a mild nucleophilic attack. Small quantities of α-1-antitrypsin can be displaced from the elastase affinity column by phenyl methane sulfonyl fluoride. In conclusion, porcine pancreatic elastase forms two complexes with α-1-antitrypsin. One or both complexes can be split by alkali. © 1976.

关键词： BzArgNan N a - benzoyl- dl -arginine -p- nitroanilide-HCl TosArgOMe p- toluene-sulfonyl- l -arginine methyl ester AcTyrOEt N- acetyl- l -tyrosine ethyl ester Ac(Ala) 3 OMe N- acetyl- l -alanyl- l -alanyl- l -alanyl methyl ester TosPheCH 2 Cl l -1-tosylamide-2-phenyl-ethylchloromethyl ketone TosLysCH 2 Cl N-α-p- tosyl- l -lysine-chloromethyl ketone-HCl SDS sodium dodecyl sulfate TEMED N , N , N ′, N ′-tetramethylethylene diamine CNBr cyanogen bromide PhMeSO 2 f phenylmethane sulfonyl fluoride

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Studies of the hydrolyses of triacylglycerols in chylomicrons, very low- and low-density lipoproteins by C-I activated lipoprotein lipase from post-heparin plasma of normal human subjects

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fEBS Letters 1976年第1期64卷 163-168页

作者： Lee, Diana M. Ganesan, Devaki Bass, Helen B. Cardiovascular Research Program Oklahoma Medical Research Foundation Oklahoma City OK 73104 825 N.E. 13th Street United States

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An in vivo effect of the metabolites l-alanine and glycyl-l-leucine on the properties of lysyl-tRNA synthetase from Escherichia coli K-12 I. Influence on subunit composition and molecular weight distribution

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BBA Section Nucleic Acids And Protein Synthesis 1976年第3期435卷 290-305页

作者： Hirshfield, Irvin N. Yeh, fu-Meei Zamecnik, Paul C. The John Collins Warren Laboratories Collis P. Huntington Memorial Hospital Harvard University Boston MA 02114 United States

Lysyl-tRNA synthetase was purified to 70-90% of homogeneity from Escherichia coli K-12. The enzyme was purified from wild-type cells grown in minimal medium, or minimal medium containing either 20 mM l-alanine or 3 mM glycyl-l-leucine. The synthetase was similarly purified from a mutant strain grown in minimal medium plus 20 mM l-alanine. Results based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gel filtration, and trypsin inactivation studies indicate (A) that the presence of l-alanine or glycyl-l-leucine in the culture medium alters the properties of the wild-type enzyme;(B) that the alteration of the synthetase by l-alanine and glycyl-l-leucine is different;and (C) that the molecular weight of lysyl-tRNA synthetase is at least 135 000-140 000. The results suggest that most likely the metabolites modify the structure of lysyl-tRNA synthetase, but the possibility that the metabolites induce the synthesis of a new lysyl-tRNA synthetase cannot be completely eliminated. © 1976.

关键词： TEMED N N N′ N′- tetramethylethylenediamine Ipr 2 P- f diisopropylfluorophosphate

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The effect of magnesium ions on action spectra for reactions mediated by Photosystems I and II in spinach chloroplasts

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BBA - Bioenergetics 1976年第2期440卷 314-321页

作者： Loos, Eckhard Fachbereich Biologie der Universität 84 Regensburg Universitatsstr. 31 Germany

Action spectra were measured for positive changes in variable fluorescence (emission > 665 nm) excited by a beam of 485 nm chopped at 75 Hz. The action of two further beams was compared, one being variable, the other (reference) constant with respect to wavelength and intensity. Comparison was achieved by alternating the reference and the variable wavelength beams at 0.3 Hz and adjusting the intensity of the latter such as to cancel out any 0.3 Hz component in the 75 Hz fluorescence signal. The relative action then was obtained as the reciprocal of the intensity of the variable wavelength beam. Similarly, action spectra were measured for O₂ evolution with ferricyanide/p-phenylenediamine as electron acceptor, and for O₂ uptake mediated by methyl viologen with ascorbate 3-(p-chlorophenyl)-1,1-dimethylurea as electron donor in the presence of 2,6-dichlorophenolindophenol. Addition of 5 mM MgCl₂ increases the relative action around 480 nm for the change in variable fluorescence and p-phenylenediamine-dependent O₂ evolution, and decreases it for methyl viologen-mediated O₂ uptake with 2,6-dichlorophenolindophenol/ascorbate as electron donor in the presence of 3-(p-chlorophenyl-1,1-dimethylurea. The change in variable fluorescence and O₂ evolution are stimulated by MgCl₂, whereas O₂ uptake is inhibited by it. The results are discussed in terms of a model assuming a tripartite organization. of the photosynthetic pigments (Thornber, J. P. and Highkin, H. R. (1974) Eur. J. Biochem. 41, 109-116;Butler, W. L. and Kitajima, M. (1975) Biochim. Biophys. Acta 396, 72-85). MgCl₂ is thought to promote energy transfer to Photosystem II from a light-harvesting pigment complex serving both photosystems. © 1976.

关键词： DCIP 2,6-dichlorophenolindophenol CMU 3-( p -chlorophenyl)-1,1-dimethylurea DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea f v fluorescence of variable yield

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ISOLATION AND CHARACTERIZATION Of DINOfLAGELLATE AND CHRYSOPHYTE CYTOCHROME-f (553-4)

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PHYTOCHEMISTRY 1975年第11期14卷 2379-2382页

作者： MEHARD, CW PREZELIN, BL HAXO, fT UNIV CALIF SAN DIEGO SCRIPPS INST OCEANOG LA JOLLA CA 92093 USA

Cytochrome- f (553–4) was isolated from mass cultures of the six dinoflagellates, Amphidinium carterae (2 strains), Cachonina niei , Glenodinium sp., Peridinium foliaceum , Gonyaulax polyedra , and one chrysophyte, Cricospherae carterae . Sonication of whole cell suspensions released the water-soluble protein, which was then purified by vacuum dialysis, salt fractionation and column chromatography. Reduced forms of isolated cytochromes had absorption maxima at 270–6, 316–8, 415–6, 522–3 and 553–4 rim. The α-absorption peak was asymmetrical. MW's, as determined by SDS polyacrylamide gel electrophoresis, ranged from 10 700 to 13 500. Amino acid analysis of C. niei cytochrome- f revealed 102 residues, with a composite MW of 10836. Purified cytochromes had isoelectric points ranging from 3·45 to 4·25 and oxidation-reduction potentials ranging from +0·374 to +0·351 V.

关键词： Amphidinium carterae Cachonina niei Glenodinium sp Peridinium foliaceum Gonyaulax polyedra dinoflagellate Cricospherae carterae chrysophyte cytochrome- f (553–4) isolation and characterization.

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AffINITY-LABELING Of ESCHERICHIA-COLI RIBOSOMES BY A DERIVATIVE Of PHENYLALANYL-TRANSfER-RNA - CRITICAL TEST fOR SPECIfICITY

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BIOCHIMICA ET BIOPHYSICA ACTA 1975年第2期395卷 146-151页

作者： BAUER, K CZERNILOfSKY, AP KUECHLER, E UNIV VIENNA INST BIOCHEMVIENNAAUSTRIA

Under nonenzymatic binding conditions p- nitrophenoxycarbonyl-phenylalanyl-tRNA can be bound to the donor as well as the acceptor site on the Escherichia coli ribosome. Precharging ribosomes with phenylalanyl-tRNA in the presence of elongation factor Tu and GTP blocks both the donor- and the acceptor site against affinity labelling by p- nitrophenoxycarbonyl-phenylalanyl-tRNA .

关键词： PNPC-Phe-tRNA p- nitrophenoxycarbonyl-phenylalanyl-tRNA PNPC-Met-tRNA Met f p- nitrophenoxycarbonyl-methionyl-tRNA Met f (formylatable, methionine specific) poly(U) poly(uridylic acid) EfTu elongation factors Tu EfTs selongation factors Ts EfG elongation factors G EfT mixture of EfTu and EfTs

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SIGNIfICANCE Of ALVEOLAR GEOMETRY AND SURfACE-TENSION IN RESPIRATORY MECHANICS Of LUNG

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RESPIRATION PHYSIOLOGY 1975年第2期24卷 115-137页

作者： REIfENRATH, R MAX PLANCK INST EXPTL MED DEPT PHYSIOL3400 GOTTINGENFED REP GER

It is customary to describe alveolar respiratory mechanics in terms of a bubble-shaped model alveolus, using the Laplace equation. The coexistence of alveoli of different radii cannot be satisfactorily explained by this model, even if hypotheses as yet unconfirmed by experiment are taken into account. Moreover, this model requires the assumption of extremely low surface tensions, near 0 dyn/cm, to explain the absence of atelectasis with very small alveolar radii and the maintenance of alveolar fluid balance. On the basis of investigations of the dynamic surface tension of lung alveolar surfactant from rat lungs, however, the minimal surface tension of alveoli is 18–20 dyn/cm. In addition, the lung does not consist of isolated and spherical alveoli but a dense packing of polyhedral air spaces separated by alveolar septa. This paper is an attempt to analyze the recoil forces of the lung due to surface tension on the basis of the polyhedral alveolar structure and the minimum surface tension mentioned above. It is demonstrated that several geometrical parameters are able to guarantee the stability of the alveolar structure of the lung to a greater extent than a variable surface tension. It is concluded that not a single (and fictive) alveolus but the septal intersections and the ‘peripheral’ septa (limiting only one air space) are the smallest morphological units of the respiratory mechanics. Some consequences concerning the pressure-volume behavior of the whole lung, the above mentioned coexistence problem and the alveolar fluid balance are discussed.

关键词： Alveolar fluid balance Dynamic surface tension Alveolar geometry Lung alveolar surfactant Respiratory mechanics Ap curved area associated with the mean excess pressure p A ps curved area of a subpleural (more general: peripheral) septum associated with the excess pressure ps ASD area/surface tension diagram f n forces due to surface tension of plane surfaces f pn average forces due to surface tension at curved surfaces f ps average force associated with the excess pressure ps of the subpleural septa f ci force due to surface tension of plane surfaces directed inward perpendicular to the pleura f(co) force due to surface tension of plane surfaces directed outward perpendicular to the pleura fp n force corresponding to the negative pleural pressure PL σ f a sum of forces acting perpendicular ‘above’ to a given plane σ f b sum of forces acting perpendicular ‘below’ to a given plane σ f i sum of the inward directed forces f ci f p and f ps σ f o sum of the outward directed forces f Co and f PL H mean curvature P K excess pressure pressure due to curvature p n mean excess pressure p s mean excess pressure of the subpleural septa r n radii of the curved surfaces at the septal intersection r s mean radius of a subpleural septum γ surface tension

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SOME ASPECTS Of INITIATION Of PROTEIN-SYNTHESIS IN CRITHIDIA-fASCICULATA AND TETRAHYMENA-PYRIfORMIS

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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 1975年第4期52卷 537-539页

作者： KIDDER, GW DEWEY, VC AMHERST COLL BIOL LABAMHERSTMA 01002

1. 1. The growth of the ciliate, Tetrahymena pyriformis , is inhibited by a number of dipeptides in the presence of suboptimal concentrations of folate. 2. 2. Its growth is also sensitive to chloramphenicol and does not occur in the absence of folate. The reverse is true of the kinetoplastid flagellate, Crithidia fasciculata . 3. 3. It is suggested that the initiation of protein synthesis in the kinetoplast-mitochondrion does not require a formylated species of aminoacyl-tRNA or that the proteins of the organelle may be cytoplasmic in origin.

关键词： tRNA f met the methionine-accepting species of transfer ribonucleic acid which is enzymatically formylatable by 10-formyltetrahydrofolate ala alanine (or alanyl etc.) asp aspartate glu glutamate gly glycine his histidine ileu isoleucine leu leucine lys lysine met methionine norleu norleucine phe phenylalanine pro proline ser serine thr threonine trp tryptophan tyr tyrosine val valine p- ABG p- aminobenzoylglutamate Protein synthesis Crithidia fasciculata Tetrahymena pyriformis peptides folate deficiency

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Non-random intrachromosomal distribution of chromatid aberrations induced by X-rays, alkylating agents and ethanol in Vicia faba

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Mutation Research - fundamental and Molecular Mechanisms of Mutagenesis 1975年第1期27卷 69-79页

作者： Rieger, R. Michaelis, A. Schubert, I. Döbel, P. Jank, H.-W. Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR Gatersleben Krs. Ascherleben German Democratic Republic

A reconstructed karyotype of Vicia faba with all chromosomes individually distinguishable was treated with triethylene melamine (TEM), cytostasan (CYT) (a new benzimidazol nitrogen mustard), mitomycin C (MI), ethanol (EA) and X-rays. The distribution within chromosomes of induced chromatid abberations was non-random for all agents. The number of segments involved in aberration clustering corresponded to the number of sites representing constitutive heterochromatin, or the regions immediately adjacent to these, as evidenced by the position of Giemsa marker bands. Which of these potential regions of aberration clustering reacted with preferential involvement in aberrations was, in part at least, dependent upon the inducing agent used. It is argued that this may be due to differences in the base composition and/or molecular conformation of heterochromatic regions. Unexpectedly, the distribution pattern of chromatid aberrations induced by mitomycin C was found to be different from those after treatment with the alkylating agents TEM and cytostasan although mitomycin C is assumed to induce aberrations via alkylation. If mitomycin C-induced aberrations are indeed due to alkylation, this indicates that different alkylating agents do not necessarily result in identical patterns of abberation clustering. The other two alkylating agents and ethanol resulted in similar patterns of preferential distribution of abberations. X-Ray induced chromatid aberrations also showed a non-random intrachromosomal distribution, but the clustering was less pronounced than after treatment with the chemical agents. © 1975.

关键词： BudR 5-bromodeoxyuridine CYT cytostasan d intercalary deletion dd duplication deletion EA ethanol i isochromatid break MH maleic hydrazide MI mitomycin C MNU N -methyl- f -nitrosourethane t chromatid translocation TEM triethylene melamine

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