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fRACTIONATION Of PHOTOCHEMICAL SYSTEMS Of PHOTOSYNTHESIS .2. CYTOCHROME AND CAROTENOID CONTENTS Of PARTICLES ISOLATED fROM SPINACH CHLOROPLASTS

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BIOCHIMICA ET BIOPHYSICA ACTA 1967年第1期143卷 187-+页

作者： BOARDMAN, NK ANDERSON, JM C.S.I.R.O. Division of Plant Industry Canberra Australia

Spinach chloroplasts were fragmented by incubation with digitonin, and the resulting particles examined for their cytochrome and carotenoid contents. Cytochrome difference spectra were recorded both at 20[degree]C and 77[degree]K, usually without prior extraction of the chlorophylls. It is concluded that at least three cytochromes are localized in the chloroplast;cytochromes f and bg with [alpha] bands at 554 and 563 m[mu] respectively, and another cytochrome with an a band at about 559 mu[mu] at 20[degree]C and 557 mu at 77[degree]K. The cytochromes are present in the approximate molar ratio of 1:1:2, respectively. It is assumed tentatively that cytochrome 559 (chloroplasts) is a b-type cytochrome. The chloroplast contains 430 molecules of chlorophyll per molecule of cytochrome f and 118 molecules of chlorophyll per molecule of cytochrome b (cytochrome b6 plus cytochrome 559). The results presented for the particles prepared by digitonin treatment of chloroplasts show that cytochromes f and bg are associated with Photosystem 1 and cytochrome 559 with Photosystem 2. Cytochromes f and bg are partly solubilized from the Photosystem 1 particle by the digitonin, and the method of preparation of the particles was modified to minimize this loss. Cytochrome 559 appears to be tightly bound to the Photosystem 2 particle. The four major carotenoids, [beta] -carotene, lutein, violaxanthin and neoxanthin were found to be present in both types of particles, but in varying proportions. The particles representative of Photosystem 1 were enriched in [beta] -carotene.

关键词： cyt f cytochrome f cyt b 6 cytochrome b 6 cyt b 3 cytochrome b 3 cyt 559 cytochrome 559 (chloroplasts) cyt b total cytochrome b (cyt b 6 plus cyt 559) chl a chlorophyll a chl b chlorophyll b

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The adsorption of sulphide ions at a mercury electrode

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Journal of Electroanalytical Chemistry and Interfacial Electrochemistry 1968年第2期16卷 219-238页

作者： R.D. Armstrong D.f. Porter H.R. Thirsk Department of Physical Chemistry University of Newcastle upon Tyne Newcastle upon Tyne 1 England

The impedance of a mercury electrode in sulphide ion solution of pH 9.5 has been studied using a.c. impedance techniques. Sulphide ions are very strongly specifically adsorbed at the electrode over a short potential range in the region of the e.c.m., leading to very high double-layer capacities. The potential-dependence of the capacity exhibits a maximum, followed at more anodic potentials by a discontinuity corresponding to the formation of a solid phase monolayer of HgS on the electrode. The frequency-dependence of the impedance in the potential region of adsorption indicates a relaxation process at a frequency of ≈7 kc/sec, and this is attributed to the slow dissociative adsorption of the HS − species according to the reaction scheme HS − agH + S 2− .

关键词： C Concentration of species S 1 (mole cm −3 ) C′ Concentration of species S 2 (mole cm −3 ) C 0 Concentration of species S 1 as x →∞ (mole cm −3 ) C′ 0 Concentration of species S 2 as x →∞ (mole cm −3 ) C b Series equivalent electrode capacity in base solution (μf cm −2 ) C i Differential capacity of the inner layer (μf cm −2 ) C p Parallel equivalent electrode capacity (μf cm −2 ) ∞ C p Parallel equivalent electrode capacity as ω→∞ (μf cm −2 ) C p = C p − ∞ C p (μf cm −2 ) C s Measured series capacity (μf cm −2 ) C s ( f →0) Series equivalent electrode capacity at low frequencies (μf cm −2 ) D Diffusion coefficient of species S 1 (cm 2 sec −1 ) D′ Diffusion coefficient of species S 2 (cm 2 sec −1 ) E Electrode potential (volts relative to Hg/HgO in 1 M NaOH solution) f frequency (c/sec) f faraday i Current density (A cm −2 ) j √−1 k, k′ first-order homogeneous rate constants (sec −1 ) k h first-order heterogeneous rate constant (cm sec −1 ) P Amplitude of sinusoidal perturbation (V) q m Charge in metal electrode (μC cm −2 ) q 1 Specifically adsorbed charge (μC cm −2 ) R p Parallel equivalent electrode resistance (Ω cm 2 ) R s Measured series resistance (Ω cm 2 ) R SO Series equivalent solution resistance (Ω) t Time (sec) V Rate of interfacial reaction (mole cm −2 sec −1 ) x Distance into solution from electrode (cm) Z Electrode impedance (Ω cm 2 ) Γ Concentration of ions adsorbed on electrode (mole cm −2 ) tan δ Loss tangent (=ω C s [ R s − R so [) tan δ Loss tangent (=[ω C p R p ] −1 ) μ Reaction zone thickness (cm) τ Life time of species S 1 (sec) Φ, Φ m Potential of metal electrode relative to bulk solution (V) Φ m −2 Potential of metal electrode relative to outer Helmholtz plane (V) Φ 0 D.c. compoentn of Φ (V) Φ 1 Potential of inner Helmholtz plane relative to bulk solution (V) ω Angular frequency (sec −1 ) ω Angular frequency at (tan δ ) −1 =1 (sec −1 )

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Potenciální náhrada vysokopecní strusky ve směsných portlandských cementech

Potenciální náhrada vysokopecní strusky ve směsných po...

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作者： Hlavinková, Eva Brno University of Technology

Předkládaná bakalářská práce se zabývá možnostmi substituce vysokopecní strusky při výrobě směsných portlandských cementů. Pozornost je zaměřena na druhotn... 详细信息

Předkládaná bakalářská práce se zabývá možnostmi substituce vysokopecní strusky při výrobě směsných portlandských cementů. Pozornost je zaměřena na druhotné suroviny, primárně na prachové podíly z výroby drceného kameniva, přičemž je posuzován jejich vliv na vlastnosti výsledného směsného pojiva.

关键词： Portlandský cement směsný cement vysokopecní struska fluidní popílek vysokoteplotní popílek drcené kamenivo odprašky provzdušňovací přísada plastifikační přísada Portland cement blended cement blast furnace slag fluidized bed combustion ash f

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STUDIES ON ACTIN-ACTIN AND ACTIN-MYOSIN INTERACTION

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BIOCHIMICA ET BIOPHYSICA ACTA 1967年第2期140卷 208-+页

作者： BAILIN, G BARANY, M Institute for Muscle Disease New York N.Y. U.S.A.

The reagents hydrogen peroxide, iodine and l,5-difluoro-2,4-dinitrobenzene did not affect the bound nucleotide content of f-actin or G-actin at all when the viscosity of the actins and their ability to interact with myosin were greatly decreased. Separation of actin-actin interaction from actin-myosin interaction was obtained by treatment of actin with hydrogen peroxide, iodine, or iodoacetamide. Iodoacetamide reacted only with cysteine residues whereas the other reagents modified cysteine, methionine and tyrosine residues in actin. When f-actin was combined with myosin forming actomyosin, methionine and tyrosine residues of f-actin were not protected in actomyosin treated with hydrogen peroxide or iodine. However, -SH groups of f-actin were protected by myosin when actomyosin was treated with these reagents. Under these conditions, f-actin isolated from actomyosin maintained its ability to interact with myosin at high and low ionic strength. The role of -SH groups of actin in the interaction of actin and myosin is discussed. An improved method is described for the isolation of actin from actomyosin.

关键词： DfDNB 1,5-difluoro-2,4-dinitrobenzene AM f actomyosin formation AM-ATPase actin-activated myosin ATPase

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Vliv obsahu pojiva na vlastnosti asfaltové směsi s CRmB

Vliv obsahu pojiva na vlastnosti asfaltové směsi s CRmB

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作者： Sychra, Martin Brno University of Technology

V diplomové práci je navržena a vyrobena asfaltová směs BBTM 5 A s asfaltem modifikovaným pryžovým granulátem (CRmB). Na této asfaltové směsi je určen vliv obsahu CRmB na n... 详细信息

V diplomové práci je navržena a vyrobena asfaltová směs BBTM 5 A s asfaltem modifikovaným pryžovým granulátem (CRmB). Na této asfaltové směsi je určen vliv obsahu CRmB na nízkoteplotní vlastnosti, moduly tuhosti a únavu asfaltové směsi BBTM 5 A. Dále byl hodnocen vliv simulace stárnutí asfaltové směsi na změnu nízkoteplotních vlastností.

关键词： Asfalt pryžový granulát asfalt modifikovaný pryžovým granulátem asfaltová směs nízkoteplotní vlastnosti modul tuhosti únava. Bitumen rubber granulate crumb rubber modified bitumen asphalt mixture low temperature properties stiffness modulus f

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Provozně technický stav objektů separace na úpravnách vody

Provozně technický stav objektů separace na úpravnách v...

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作者： Pešout, Jakub Brno University of Technology

Úkolem této diplomové práce bylo stanovení provozně technických parametrů separačních objektů na úpravnách vody. V první části se nachází teoretick... 详细信息

Úkolem této diplomové práce bylo stanovení provozně technických parametrů separačních objektů na úpravnách vody. V první části se nachází teoretická část poskytující základní informace k této problematice. V druhé části se nachází stanovená hodnotící kritéria podle získaných informací k jednotlivým objektům a získaných v praxi prostřednictvím osobních prohlídek na vybraných úpravnách vody.V poslední části se nachází aplikace těchto kritérií na úpravnách vody v praxi s celkovým hodnocením jejich stavu.

关键词： Úpravna vody sedimentace filtrace flotace provozně technické parametry koagulace flokulace pitná voda podzemní vody povrchové vody. Water treatment plant sedimentation filtration flotation operational and technical parameters coagulation f

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SOME PHYSICAL AND CHEMICAL PROPERTIES Of THE LIPOPROTEINS PRODUCED BY LIPOLYSIS Of HUMAN SERUM Sf20-400 LIPOPROTEINS BY POST-HEPARIN PLASMA

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JOURNAL Of ATHEROSCLEROSIS RESEARCH 1962年第1-2期2卷 104-114页

作者： SHORE, B SHORE, V Department of Physiology Washington University School of Medicine St. Louis Missouri (U.S.A.)

After human serum S f 20–400 lipoproteins were incubated with post-heparin plasma, the principal product of lipolysis was S f 11–20 (density D = 1.006–1.022 g/ml), lipoproteins. Their properties closely resembled those of molecules of comparable density among the normal human serum low density lipoproteins ( D < 1.065 g/ml). It is likely that each NH 2 -terminal amino-acid of the substrate represents the protein moiety of a lipoprotein or family of lipoproteins varying in lipid content, and that all these lipoproteins of the substrate contain triglyceride in a form susceptible to hydrolysis by post-heparin plasma. The S f 11–20 ( D = 1.006–1.022 g/ml), the S f 0–3 ( D = 1.045–1.065 g/ml), and perhaps the S f 20–400 ( D = 0.98–0.99 g/ml) lipoproteins of human serum contain molecules with the electrophoretic mobility of α 2 -globulins, whereas the S f 4–8, ( D = 1.031–1.035 g/ml) lipoproteins contain molecules with the mobility of β-globulins. Aprés incubation de lipoprotéines S f 20–400 provenant du sérum humain avec du plasma posthéparinique le produit principal de la lypolyse était des lipoprotéines S f 11–20 (densité, D = 1.006–1.022 g/ml). Leurs propriétés ressemblent bien à celles de molécules à densité comparable parmi les lipoprotéines à densité basse ( D < 1.065 g/ml) du sérum humain normal. Probablement, chaque aside aminé NH 2 -terminal du substrat représente la part des protéines d'une lipoprotéine ou d'une famille de lipoprotéines à teneur variable en lipides, et toutes ces lipoprotéines du substrat contiennent de la triglycéride sous une forme susceptible d'être hydrolysée par du plasma posthéparinique. Les lypoprotéines du sérum humain S f 11–20 (D = 1.006–1.022 g/ml), S f 0–3 ( D = 1.045–1.065 g/ml), et peut-être S f 20–400 ( D = 0.98–0.99 g/ml) contiennent des molecules ayant la mobilite électrophorétique de globulines-α 2 , tandis que les lipoprotéines S f 4–8 ( D = 1.031–1.035 g/ml) renferment des molécules ayant la mobilité de globulines-β. Nach In

关键词： S f flotation coefficient, the negative of the sedimentation coefficient in Svedberg units of species undergoing centripetal migration in a NaCl solution of density 1.0653 g/ml at 20°C LDL low density lipoproteins, lipoproteins of hydrated density less than 1.065 g/ml HDL high density lipoproteins, lipoproteins of hydrated density 1.065-1.20 g/ml. D density tris tris (hydroxymethyl)-aminomethane EDTA ethylenediamine tetraacetic acid

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MEASUREMENTS Of LINEAR POLARIZATION Of ELASTICALLY SCATTERED GAMMA RAYS

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NUCLEAR PHYSICS 1965年第3期64卷 502-&页

作者： SINGH, M SOOD, BS Physics Department Punjabi University Patiala India

Measurements are described of the linear polarization of elastically scattered gamma rays from a lead scatterrer. The results indicate that an incomplete resolution of incoherent scattering from coherent scattering tends to lower the values of the observed polarization. The measured values of the polarization of the elastic scattering of 280, 662 and 1250 keV gamma rays at different scattering angles are found to agree with the theoretical values of Brown and Mayers. In contradiction with earlier suggestions, it is found that the effect of L shell contributions is small and that it can not account for the large deviations reported previously. The present results remove the discrepancy between the experiment and theory.

关键词： ATOMIC PHYSICS Pb(γ, γ), E = 280, 662, 1250 keV measured linear polarization as f ( θ )

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PURIfICATION Of fLUORESCEIN AND fLUORESCEIN DERIVATIVES BY CELLULOSE ION EXCHANGE CHROMATOGRAPHY

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IMMUNOCHEMISTRY 1967年第4期4卷 191-+页

作者： DANDLIKER, WB ALONSO, R Division of Biochemistry Scripps Clinic and Research Foundation La Jolla California USA

A method for the purification of ionic dyes by cellulose ion exchangers is presented. The underlying rationale is based upon approximate knowledge of the pK''s of the ionizing groups of the dye. fluorescein purified by DEAE cellulose chromatography has a molar extinction coefficient of 8.72 x 104 in 0.01 [image] NaOH at 491 m[mu]. This agrees with results obtained by other workers using different methods of purification. fluorescein amine is readily purified on a 10 g scale by a similar method and an improved procedure for converting the amine to the isothiocyanate is given. The amine is a convenient and stable compound which can be standardized, stored and converted to the isothiocyanate as needed for fluorescent labeling.

关键词： f fluorescein fNO 2 nitrofluorescein fNH 2 fluorescein amine fNCS fluorescein isothiocyanate DEAE diethylaminoethyl AcOH acetic acid NH 4 OAc ammonium acetate

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MULTIPLE MOLECULAR fORMS Of XANTHINE DEHYDROGENASE AND RELATED ENZYMES .2. CONVERSION Of ONE fORM Of XANTHINE DEHYDROGENASE TO ANOTHER BY EXTRACTS Of DROSOPHILA MELANOGASTER

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BIOCHIMICA ET BIOPHYSICA ACTA 1968年第2期160卷 178-&页

作者： SHINODA, T GLASSMAN, E Department of Biochemistry and The Genetics Curriculum The University of North Carolina Chapel Hill N.C. U.S.A.

1. 1. Xanthine dehydrogenase of Drosophila melanogaster exists predominantly as one form, XDH-I, in fresh homogenates. However, a second form, XDH-II, is found in partially purified extracts. The two forms of xanthine dehydrogenase are separable on DEAE-cellulose columns and by polyacrylamide gel electrophoresis indicating that each has a different charge. XDH-I sediments slightly faster than XDH-II during sucrose gradient centrifugation indicating a small difference in molecular weight or in conformation. Other properties of the molecules are not too different. 2. 2. XDH-I can be converted to a form like XDH-II by incubation with appropriate Drosophila extracts. Preliminary analysis indicates that the converter molecule has the properties of a large protein. 3. 3. The properties of XDH-II mimic the properties of xanthine dehydrogenase from flies grown on a high protein diet. These flies also have 3- to 5-fold levels of xanthine dehydrogenase activity but no increase in xanthine dehydrogenase antigenicity (cross-reacting material against xanthine dehydrogenase antibody). It is tempting to speculate that the conversion of XDH-I to XDH-II studied in this paper represents an process in vitro related to the regulation in vivo of xanthine dehydrogenase in Drosophila.

关键词： XDH-I one form of xanthine dehydrogenase XDH-II another form of xanthine dehydrogenase OR the Oregon-R wild type strain of D. melanogaster PAC the Pacific wild type strain of D. melanogaster Pm a strain of D. melanogaster containing Pm Sb/Xa ma − l a strain containing v f Bx 3 ma − l ry a strain containing st ry

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