目的探讨18f-Na f PET/CT在前列腺癌骨转移中的诊断价值及影像学特征。方法回顾性分析42例经病理证实为前列腺癌的患者的临床资料,均行18f-Na f PET/CT骨显像。比较18f-Na f PET/CT、18f-Na f PET和单独CT诊断前列腺癌骨转移灶的灵敏性...
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目的探讨18f-Na f PET/CT在前列腺癌骨转移中的诊断价值及影像学特征。方法回顾性分析42例经病理证实为前列腺癌的患者的临床资料,均行18f-Na f PET/CT骨显像。比较18f-Na f PET/CT、18f-Na f PET和单独CT诊断前列腺癌骨转移灶的灵敏性、准确性。结果18f-Na f PET/CT、18f-Na f PET、单独CT诊断前列腺癌骨转移瘤的灵敏度分别为100%、94.4%、77.8%,准确性分别为95.2%、87.0%、71.4%。18f-Na f PET/CT骨显像的准确性显著高于18f-Na f PET和单独CT(P<0.05)。结论18f-Na f PET/CT对前列腺癌骨转移诊断的灵敏度、特异性、准确性较高。
目的探讨白细胞介素-33(IL-33)在粉尘螨1类变应原(Der f 1)诱导的过敏性哮喘小鼠中的变化及作用。方法将40只雌性BALB/c小鼠用随机数字表法分为哮喘组、免疫治疗组、抑制剂组和阴性对照组(PBS组),每组10只。哮喘组、免疫治疗组和抑制剂...
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目的探讨白细胞介素-33(IL-33)在粉尘螨1类变应原(Der f 1)诱导的过敏性哮喘小鼠中的变化及作用。方法将40只雌性BALB/c小鼠用随机数字表法分为哮喘组、免疫治疗组、抑制剂组和阴性对照组(PBS组),每组10只。哮喘组、免疫治疗组和抑制剂组分别在第0、7和14天腹腔注射100μl粉尘螨变应原提取液(含100μg/ml Der f 1),抑制剂组小鼠同时注射抑制剂(可溶性ST2 100μl,用于抑制IL-33),PBS组注射等量PBS。第21天起,各组小鼠雾化吸入粉尘螨变应原提取液(0.5μg/ml),30 min/次,1次/d,连续7 d。免疫治疗组小鼠第25天起于雾化前0.5 h,分别用200μl的Der f 1蛋白溶液(100μg/ml)腹腔注射进行免疫治疗,PBS组用PBS代替粉尘螨变应原。第27天雾化吸入后24 h处死小鼠,收集小鼠支气管肺泡灌洗液(BALf)和眼球血,并对BALf中嗜酸粒细胞进行计数,制作肺组织病理切片。ELISA法检测BALf中IL-5、IL-13和γ干扰素(IfN-γ)的含量及血清中特异性Ig E和Ig G2a水平。结果除PBS组小鼠外,其他组小鼠自第21天开始出现不同程度的哮喘症状。自第25天开始,免疫治疗组小鼠的哮喘症状开始缓解。哮喘组和PBS组小鼠BALf中嗜酸粒细胞总数分别为(4.41±0.36)×10~5/ml和(0.37±0.08)×10~5/ml,两组差异有统计学意义(t=24.50,P<0.01);免疫治疗组和抑制剂组小鼠的嗜酸粒细胞总数分别为(1.43±0.14)×10~5/ml和(2.73±0.33)×10~5/ml,均明显低于哮喘组(f=129.72,P<0.01)。ELISA检测结果显示,哮喘组、免疫治疗组、抑制剂组和PBS组BALf中IfN-γ的水平分别为(83.06±11.38)、(277.97±22.46)、(175.13±13.41)和(224.77±19.97)pg/ml,免疫治疗组和抑制剂组均明显高于哮喘组(t=17.31,t=11.71,P<0.01)。哮喘组、免疫治疗组、抑制剂组和PBS组小鼠BALf中IL-5水平分别为(208.64±11.55)、(106.87±11.39)、(140.71±14.58)和(90.15±9.49)pg/ml,哮喘组的水平明显高于其他各组(f=97.19,P<0.01)。哮喘组和免疫治疗组BALf中IL-13水平分别为(308.37±13.67)pg/ml和(175.66±11.79)pg/ml,两者差异有统计学意义(P<0.01);抑制剂组小鼠的为(221.12±21.08)pg/ml,明显低于哮喘组(t=16.44,P<0.01);PBS组为(97.57±18.38)pg/ml。免疫治疗组与哮喘组相比,小鼠肺部炎性症状均减轻,几乎无炎症细胞。抑制剂组小鼠的支气管周围也有嗜酸粒细胞增多症、上皮细胞脱落和支气管上皮细胞肥大,但较哮喘组要减轻许多。血清Ig E抗体水平的检测结果显示,哮喘组、免疫治疗组、抑制剂组和PBS组分别为(31.97±3.48)、(12.86±2.22)、(18.43±2.30)和(9.68±1.27)IU/ml,免疫治疗组和抑制剂组均低于哮喘组(t=-7.77,P<0.01)。Ig G2a抗体水平的变化和Ig E相反,哮喘组、免疫治疗组和PBS组分别为(26.94±2.96)、(35.06±2.57)和(10.31±1.48)μg/ml,免疫治疗组高于哮喘组(t=6.55,P<0.01)。结论 ELISA检测结果表明IL-33/ST2信号通路在小鼠过敏性哮喘中发挥着重要的作用。
Embolization of amniotic fluid (Af) into the blood circulation leads to disseminated intravascular coagulation (DIC). Procoagulant phosphatidylserine (PS)- and tissue factor (Tf)-exposing extracellular vesicles (EVs) ...
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Embolization of amniotic fluid (Af) into the blood circulation leads to disseminated intravascular coagulation (DIC). Procoagulant phosphatidylserine (PS)- and tissue factor (Tf)-exposing extracellular vesicles (EVs) might play an important role in Af embolism-induced DIC. It was the aim of the present study to perform analyses of the procoagulant properties of Af with a panel offunctional coagulation assays and flow cytometry. We applied a prothrombinase assay (that quantifies PS exposure on EVs), an EV-associated Tf activity assay, a fibrin generation assay, a thrombin generation assay, a whole blood clotting model, and flow cytometry in Af and control plasma. We found that PS exposure on EVs was 21-fold increased in Af compared with plasma. Also, EV-associated Tf activity was highly increased in Af compared with plasma. Af-derived EVs activated the blood coagulation cascade via PS and Tf in the fibrin and thrombin generation assays. In a whole blood clotting model, Af-derived EVs significantly shortened the clotting time from 734 139 seconds in the presence to 232 139 seconds in the absence of an anti-If antibody. The contact activation pathway via factor XII (fXII) was not affected. Applying flow cytometry, a sub population of PS+ and Tf+ EVs was identified in Af but not in control plasma. In conclusion, we investigated the effect of Af on blood coagulation and found that PS+ and Tf+ EVs determine their procoagulant potential. Taken together, our data further delineate the pathomechanisms underlying Af-induced coagulopathy.
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As shown in the previous article, two distinct conjugative plasmids sometimes interact within bacterial cells, implicating changes of transfer rates. In most cases of interactions within bacteria, the transfer of one ...
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As shown in the previous article, two distinct conjugative plasmids sometimes interact within bacterial cells, implicating changes of transfer rates. In most cases of interactions within bacteria, the transfer of one of the plasmids decreases. Less frequently, the transfer rate of one of the plasmids increases. Here we analyse what happens if three distinct conjugative plasmids colonize the same bacterial cell. Our aim is to understand how interactions between two plasmids affect the transfer rate of the third plasmid. After showing that plasmids interact in 59 out of 84 possible interactions we show that, with some exceptions, if the transfer rate of a plasmid decreases in the presence of a second plasmid, a decrease is also observed in the presence of a third plasmid. Moreover, if the conjugation rate of a plasmid increases in the presence of another, an increase is also observed if there is a third plasmid in the cell. Both types of interactions are mostly independent of the third plasmid's identity, even if sometimes the third plasmid quantitatively distorts the interaction of the other two plasmids. There is a bias towards negative intensifying interactions, which provide good news concerning the spread conjugative plasmids encoding antibiotic-resistance genes and virulence factors.
The ability to recognize faces varies considerably between individuals, but does performance co-vary for tests of different aspects offace processing? for 397 participants (of whom the majority were university studen...
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The ability to recognize faces varies considerably between individuals, but does performance co-vary for tests of different aspects offace processing? for 397 participants (of whom the majority were university students) we obtained scores on the Mooney face Test, Glasgow face Matching Test (GfMT), Cambridge face Memory Test (CfMT) and Composite face Test. Overall performance was significantly correlated for each pair of tests, and we suggest the term ffor the factor underlying this pattern of positive correlations. However, there were large variations in the amount of variance shared by individual tests: The GfMT and CfMT are strongly related, whereas the GfMT and the Mooney test tap largely independent abilities. We do not replicate a frequently reported relationship between holistic processing (from the Composite test) and face recognition (from the CfMT)-indeed, holistic processing does not correlate with any of our tests. We report associations of performance with digit ratio and autism-spectrum quotient (AQ), and from our genome-wide association study we include a list of suggestive genetic associations with performance on the four face tests, as well as with f. (C) 2017 Elsevier Ltd. All rights reserved.
目的对粉尘螨的过敏原蛋白之一的der f 28的结构与性质进行生物信息方面的分析,为粉尘螨所导致的变态反应性疾病的诊断和治疗提供线索。方法通过对der f 28进行BLAST得到的相似序列进行构建进化树,同时对der f28的二级结构、亲水性区域...
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目的对粉尘螨的过敏原蛋白之一的der f 28的结构与性质进行生物信息方面的分析,为粉尘螨所导致的变态反应性疾病的诊断和治疗提供线索。方法通过对der f 28进行BLAST得到的相似序列进行构建进化树,同时对der f28的二级结构、亲水性区域、可塑性区域、抗原性区域进行分析。结果粉尘螨与其他微生物物种中的热休克蛋白70（HSP70）在进化上相似性不高。der f 28的主要抗原位点为24~39、93~103、150~163、202~205、244~281、414~434、450~484、551~605。Motif的预测表明der f 28具有热休克蛋白70家族的特征。其预测的三维结构基本能反应der f 28真实的空间构象。结论掌握der f 28结构和功能的的关系,对粉尘螨所引起的变态反应性疾病的诊断和治疗有临床意义。
Conjugative plasmids encode the genes responsible for the synthesis of conjugative pili and plasmid transfer. Expression of the conjugative machinery (including conjugative pili) may be costly to bacteria, not only du...
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Conjugative plasmids encode the genes responsible for the synthesis of conjugative pili and plasmid transfer. Expression of the conjugative machinery (including conjugative pili) may be costly to bacteria, not only due to the energetic/metabolic cost associated with their expression but also because they serve as receptors for certain viruses. Consequently, the presence of two plasmids in the same cell may be disadvantageous to each plasmid, because they may impose a higher fitness cost on the host. Therefore, plasmids may encode mechanisms to cope with co-resident plasmids. Moreover, it is possible that the transfer rate of a plasmid is affected by the presence of a distinct plasmid in the recipient cell. In this work, we measured transfer rates of twelve natural plasmids belonging to seven incompatibility groups in three situations, namely when: (i) donor cells contain a plasmid and recipient cells are plasmid-free;(ii) donor cells contain two unrelated plasmids and recipient cells are plasmidfree;and (iii) half of the cells contain a given plasmid and the other half contain another, unrelated, plasmid. In the third situation, recipient cells of a plasmid are the donor cells of the other plasmid. We show that there are more negative interactions (reduction of a plasmid's conjugative efficiency) between plasmids if they reside in the same cell than if they reside in different cells. However, if plasmids interacted intercellularly, the transfer rate of one of the plasmids was often higher (when the unrelated conjugative plasmid was present in the recipient cell) than if the recipient cell was plasmid-free a positive effect. Experimental data retrieved from the study of mutant plasmids not expressing conjugative pili on the cell surface suggest that positive effects result from a higher efficiency of mating pair formation. Overall, our results suggest that negative interactions are significantly more frequent when plasmids occupy the same cell. Such interactions may
目的:制备有生物学活性的粉尘螨过敏原蛋白Der f 3。方法:构建带有StrepⅡ标签的原核表达体系Rosetta-p ET44a-Der f 3高效表达目的蛋白,分别用梯度透析法和亲和层析法对其进行复性纯化,通过Western Blot试验验证目的蛋白的有效表达,最...
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目的:制备有生物学活性的粉尘螨过敏原蛋白Der f 3。方法:构建带有StrepⅡ标签的原核表达体系Rosetta-p ET44a-Der f 3高效表达目的蛋白,分别用梯度透析法和亲和层析法对其进行复性纯化,通过Western Blot试验验证目的蛋白的有效表达,最后用丝氨酸蛋白酶特异荧光底物及粉尘螨过敏病人血清分别鉴定目的蛋白的酶学活性及免疫学活性。结果:利用原核表达体系成功表达了目的蛋白Der f 3,Western Blot结果显示有目的条带出现。复性纯化后的过敏原蛋白Der f 3能够成功降解丝氨酸蛋白酶特异性荧光底物,ELISA试验结果显示该蛋白血清特异性Ig E在4级以上粉尘螨过敏病人中阳性率为4%。结论:成功获得有酶学活性及免疫学活性的粉尘螨过敏原蛋白Der f 3,为生产高质量的诊断试剂和疫苗奠定了基础。
Ostracods are described for the first time from the Late Devonian of western Junggar in Xinjiang, NW China. fifty-two species belonging to 30 genera are recognized, and seven are new: Arcuaria hebukesarensis sp. nov.,...
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Ostracods are described for the first time from the Late Devonian of western Junggar in Xinjiang, NW China. fifty-two species belonging to 30 genera are recognized, and seven are new: Arcuaria hebukesarensis sp. nov., Bairdia shaerbuertiensis sp. nov., Cribroconcha honggulelengensis sp. nov., Microchelinella bulongourensis sp. nov., M. hoxtolgayensis sp. nov., Pribylites wulankeshunensis sp. nov. and P. junggarensis sp. nov. The ostracod fauna indicates a probable late frasnian age for the lower member of the Hongguleleng formation, and the frasnian/famennian boundary may exist in the basal part of the formation. The ostracod assemblages are referable to the Eifelian Mega-Assemblage, incorporating both the palaeocopid and smooth-podocopid associations. The fauna implies deposition in a nearshore-offshore environment during a transgression when the lower member of the Hongguleleng formation was being deposited in western Junggar.
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