Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening...
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Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening method based on a high-throughput optical array system assembled by multiple cholinesterases (ChE) and thi-ocholine (TCh) sensors is described. The detection mechanism is that the inhibition of ChE activity by pesticides reduces the TCh produced by the hydrolysis of butyryl/acetylthiocholine iodide, thus changing the fluorescence intensity of TCh sensor. The diverse response of ChEs to pesticides and different affinity of sensors to TCh ensure the high-throughput and distinguishable signal output, which allow the establishment of high discrete pesticide database with intra-cluster agglomeration and inter-cluster dispersion. By using the database, the screening of unknown real contaminated samples were successfully operated, and the screened pesticide species and concentrations were consistent with high-performance liquid chromatography. This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.
The precise recognition and sensing of steroids, a type of vital biomolecules, hold immense practical value across various domains. In this study, we introduced corral[4]BINOLs ( C[4]BINOL s), a pair of enantiomeric c...
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The precise recognition and sensing of steroids, a type of vital biomolecules, hold immense practical value across various domains. In this study, we introduced corral[4]BINOLs ( C[4]BINOL s), a pair of enantiomeric conjugated deep-cavity hosts, as novel synthetic receptors for binding steroids. Due to the strong hydrophobic effect of their deep nonpolar, chiral cavities, the two enantiomers of C[4]BINOL s demonstrated exceptionally high recognition affinities (up to 10 12 M −1 ) for 16 important steroidal compounds as well as good enantioselectiviy (up to 15.5) in aqueous solutions, establishing them as the most potent known steroid receptors. Harnessing their ultrahigh affinity, remarkable enantioselectivity, and fluorescence emission properties, the two C[4]BINOL enantiomers were employed to compose a fluorescent sensor array which achieved discrimination and sensing of 16 structurally similar steroids at low concentrations.
Enormous efforts have been paid to detection of various biothiols due to their involvements in many physiological processes. In the present work, we reported a simple fluorescent sensor array for fingerprinting four d...
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Enormous efforts have been paid to detection of various biothiols due to their involvements in many physiological processes. In the present work, we reported a simple fluorescent sensor array for fingerprinting four different biothiols based on the same probe. The probe was a dual-fluorophore containing pyrene-pyronine FRET pair and its fluorescence emission could be easily modulated by CTAB surfactant assemblies. The three-element sensorarray was built by simply varying CTAB concentration in the sensor elements that contained the same probe. The array can produce unique fluorescence fingerprints for four different biothiols and be applied to discriminate them even in biofluids like serum and urine. This study demonstrates the surfactant modulation strategy associated with using a FRET dual-fluorophore can offer an opportunity to build simple and effective fingerprinting sensing platform for multiple biothiols.
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