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Role of biotin carboxyl carrier protein subunit 2 (BCCP2) in resistance to multiple stresses in Arabidopsis thaliana

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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2025年 745卷 151232页

作者： Wang, Yao Wang, Min Ye, Xiaoxue Shen, Huifang Zhao, Rui Wu, Jiawu Wang, Fei Shen, Xinting Li, Zhebin Zhou, Ye Li, Bo Yao, Xinmiao Lu, Shuwen Heilongjiang Acad Agr Sci Food Proc Res Inst Harbin 150086 Peoples R China Heilongjiang Prov Key Lab Food Proc Harbin 150086 Peoples R China Heilongjiang Acad Agr Sci Inst Agr Remote Sensing & Informat Harbin 150086 Peoples R China Chinese Acad Trop Agr Sci Inst Trop Biosci & Biotechnol Sanya Res Inst Haikou 570102 Peoples R China Heilongjiang Acad Agr Sci Harbin 150086 Peoples R China

Abiotic stresses, including drought, salinity, and temperature extremes, are serious constraints to plant growth and agricultural development. These stresses that plants face in nature are often multiple and complex. Biotin carboxyl carrier protein subunit 2 (BCCP2) is one of the two subunits of biotin carboxyl carrier protein, which is a functional subunit of acetyl coenzyme A carboxylase, primarily studied for its role in fatty acid synthesis. In this study, we identified the expression pattern of AtBCCP2 under various stress conditions, including 4 mM NaHCOs, 2 mM NaaCOs, 150 mM NaCl, 300 mM D-mannitol, 100 mu M ABA, 5 mM HaOa, 4 degrees C, and 37 degrees C. It was determined that AtBCCP2 is positively regulated by NaHCOs, NaaCOs, NaCl, and ABA, but negatively regulated by Dmannitol. Phenotypic experiment confirmed that the AtBCCP2 transgenic overexpression plants exhibited increased resistance to NaHCOs, NaaCOs, NaCl, and ABA stresses, but more sensitive to drought stress simulated by D-mannitol. In contrast, mutant plants showed the opposite phenotypes. Additionally, AtBCCP2 transgenic overexpression plants demonstrated stronger antioxidant activity and lower MDA content under stresses such as NaHCOs, NaaCOs, NaCl, and ABA, in contrast to the mutant plants. In response to D-mannitol simulated drought stress, AtBCCP2 transgenic overexpression plants showed lower antioxidant activity and higher MDA content, while mutant plants exhibited the opposite trend. In conclusion, this study provides a theoretical basis for understanding the role of AtBCCP2 in response to multiple stresses and contributes a new gene to the pool of those involved in abiotic stress responses.

关键词： Arabidopsis thaliana Multiple stresses gene expression pattern Plant phenotype Antioxidant enzyme activity

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Acyl-CoA-binding protein (ACBP) genes involvement in response to abiotic stress and exogenous hormone application in barley (Hordeum vulgare L.)

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BMC PLANT BIOLOGY 2024年第1期24卷 236页

作者： Chang, Huayu Ma, Minhu Gu, Mingzhou Li, Shanshan Li, Mengrun Guo, Ganggang Xing, Guofang Shanxi Agr Univ Coll Agr Hou Ji Lab Shanxi Prov Taiyuan 030031 Shanxi Peoples R China Chinese Acad Agr Sci ICS CAAS Inst Crop Sci Key Lab Grain Crop Genet Resources Evaluat & Utili State Key Lab Crop Gene Resources & Breeding Beijing 100081 Peoples R China

Background Acyl-CoA-Binding proteins (ACBPs) function as coenzyme A transporters and play important roles in regulating plant growth and development in response to abiotic stress and phytohormones, as well as in membrane repair. To date, the ACBP family has not been a comprehensively characterized in barley (Hordeum vulgare L.). Results Eight ACBP genes were identified in the barley genome and named as HvACBP1-8. The analysis of the proteins structure and promoter elements of HvACBP suggested its potential functions in plant growth, development, and stress response. These HvACBPs are expressed in specific tissues and organs following induction by abiotic stressors such as drought, salinity, UV-B exposure, temperature extremes, and exposure to exogenous phytohormones. The HvACBP7 and HvACBP8 amino acid sequences were conserved during the domestication of Tibetan Qingke barley. Conclusions Acyl-CoA-binding proteins may play important roles in barley growth and environmental adaptation. This study provides foundation for further analyses of the biological functions of HvACBPs in the barley stress response.

关键词： Barley stress response Acyl-CoA-binding protein (ACBP) gene expression pattern

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Genome-Wide Identification and expression Analysis of the HSF gene Family in Poplar

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FORESTS 2023年第3期14卷 510页

作者： Zhao, Kai Dang, Hui Zhou, Lieding Hu, Jia Jin, Xia Han, Youzhi Wang, Shengji Shanxi Agr Univ Coll Forestry Jinzhong 030801 Peoples R China Inner Mongolia Agr Univ Coll Forestry Hohhot 010018 Peoples R China

The Heat Shock Factor (HSF) transcription factor family plays crucial roles in plant growth and development, as well as in protecting against adverse stresses. However, studies on the functions and regulatory mechanisms of the HSF genes are limited in poplar. Here, we identified and classified 30 HSF transcription factors in Populus trichocarpa based on recent genomic data and annotation information and conducted a comprehensive analysis of these proteins, including phylogenetic and physicochemical properties analysis, domain characterization, subcellular localization prediction, cis-acting elements analysis, sequence structure analysis, and chromosomal distribution. Our analysis revealed that segmental duplication events may be the main driving force behind the expansion of the poplar HSF gene family, and we explored the collinearity between poplar HSF genes and those of six other representative species. We also analyzed the tissue-specific and hormonal responses of the HSF genes in poplar and conducted gene co-expression network analysis, which revealed important molecular functions and biological processes related to growth and development, biotic and abiotic stress response, and epigenetic modification. These results provide significant insights into the functions and regulatory mechanisms of the HSF genes in poplar.

关键词： poplar HSF gene family gene expression pattern phytohormones co-expression analysis

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Genome-wide identification of PYL/PYR-PP2C (A)-SnRK2 genes in Eutrema and their co-expression analysis in response to ABA and abiotic stresses

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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2023年第Part2期253卷 126701页

作者： Li, Chuanshun Zhang, Hengyang Qi, Yuting Zhao, Yaoyao Duan, Chonghao Wang, Yujiao Meng, Zhe Zhang, Quan Shandong Normal Univ Coll Life Sci Shandong Prov Key Lab Plant Stress Res Jinan Peoples R China Shandong Normal Univ Coll Life Sci Res Team Plant Pathogen Microbiol & Immunol Jinan Peoples R China

ABA signaling core components PYR/PYL, group A PP2C and SnRK2 play important roles in various environmental stress responses of plants. This study identified 14 PYR/PYL, 9 PP2C (A), and 10 SnRK2 genes from halophytic Eutrema. Phylogenetic analysis showed 4 EsPYR/PYL, 4 EsPP2C (A) and 3 EsSnRK2 subfamilies characterized, which was supported by their gene structures and protein motifs. Large-scale segmental duplication event was demonstrated to be a major contributor to expansion of the EsPYL-PP2C (A)-SnRK2 gene families. Synteny relationship analysis revealed more orthologous PYL-PP2C (A)-SnRK2 gene pairs located in collinear blocks between Eutrema and Brassica than that between Eutrema and Arabidopsis. RNA-seq and qRT-PCR revealed EsABI1, EsABI2 and EsHAL2 showed a significantly up-regulated expression in leaves and roots in response to ABA, NaCl or cold stress. Three markedly co-expression modules of ABA/R-brown, NaCl/L-lightsteelblue1 and Cold/R-lightgreen were uncovered to contain EsPYL-PP2C (A)-SnRK2 genes by WGCNA analysis. GO and KEGG analysis indicated that the genes of ABA/R-brown module containing EsHAB1, EsHAI2 and EsSnRK2.6 were enriched in proteasome pathway. Further, EsHAI2-OE transgenic Arabidopsis lines showed significantly enhanced seeds germination and seedlings growth. This work provides a new insight for elucidating potential molecular functions of PYL-PP2C (A)-SnRK2 responding to ABA and abiotic stresses.

关键词： Eutrema salsuginea Abscisic acid EsPYL-PP2C (A)-SnRK2 Abiotic stress response Collinearanalysis gene expression pattern gene co-expression network Proteasome pathway

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Spatiotemporal expression patterns of cytosolic AtHSP90-2 in Arabidopsis seedlings

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PLANT SIGNALING & BEHAVIOR 2023年第1期18卷

作者： Kozeko, Liudmyla Kordyum, Elizabeth Natl Acad Sci Ukraine MG Kholodny Inst Bot Dept Cell Biol & Anat Tereshchenkivska Str 2 UA-01601 Kiev Ukraine

Heat shock protein AtHSP90-2 is one of the three constitutive cytosolic HSP90s of Arabidopsis thaliana, which are highly homologous and show mild expression activation in response to stressful impacts. To characterize the functioning of AtHSP90-2, we have analyzed tissue-specificity of its expression during seedling development using a DsG transgenic line carrying a loss-of-function mutation of AtHSP90-2 via translational fusions with the beta-glucuronidase reporter gene (GUS). Histochemical analysis during the first two weeks of seedling growth revealed AtHSP90-2 expression in all organs, as well as differences in its intensity between tissues and showed its dynamics. The tissue-specific AtHSP90-2-GUS expression pattern was shown to be maintained under heat shock and water deficit. The most prominent GUS staining was detected in the vascular system and hydathodes of cotyledons, and stipules. The basipetal gradient of AtHSP90-2 expression during leaf formation, its dynamics in developing stipules, and the high level of its expression in cells with active transport function suggest a special role for the gene in certain cellular processes.

关键词： Arabidopsis AtHSP90-2 gene expression pattern developmental regulation stress

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A Small Change With a Twist Ending: A Single Residue in EGF-CFC Drives Bilaterian Asymmetry

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MOLECULAR BIOLOGY AND EVOLUTION 2023年第2期40卷 msac270-msac270页

作者： Truchado-Garcia, Marta Perry, Kimberly J. Cavodeassi, Florencia Kenny, Nathan J. Henry, Jonathan Q. Grande, Cristina Univ Autonoma Madrid Fac Ciencias Dept Biol Madrid Spain Univ Calif Berkeley Dept Mol & Cell Biol 571 Weill Hall Berkeley CA 94702 USA Univ Illinois Dept Cell & Dev Biol Urbana IL 61801 USA St Georges Univ London Inst Med & Biomed Educ Cranmer Terrace London SW17 0RE England Nat Hist Museum Cromwell Rd London England Univ Otago Dept Biochem Te Tari Matu Koiora Dunedin New Zealand Marine Biol Lab Woods Hole MA 02543 USA

Asymmetries are essential for proper organization and function of organ systems. genetic studies in bilaterians have shown signaling through the Nodal/Smad2 pathway plays a key, conserved role in the establishment of body asymmetries. Although the main molecular players in the network for the establishment of left-right asymmetry (LRA) have been deeply described in deuterostomes, little is known about the regulation of Nodal signaling in spiralians. Here, we identified orthologs of the egf-cfc gene, a master regulator of the Nodal pathway in vertebrates, in several invertebrate species, which includes the first evidence of its presence in non-deuterostomes. Our functional experiments indicate that despite being present, egf-cfc does not play a role in the establishment of LRA in gastropods. However, experiments in zebrafish suggest that a single amino acid mutation in the egf-cfc gene in at least the common ancestor of chordates was the necessary step to induce a gain of function in LRA regulation. This study shows that the egf-cfc gene likely appeared in the ancestors of deuterostomes and "protostomes", before being adopted as a mechanism to regulate the Nodal pathway and the establishment of LRA in some lineages of deuterostomes.

关键词： Nodal EGF-CFC cripto left-right asymmetry zebrafish EvoDevo gene expression pattern Spiralia Crepidula fornicata

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Genome-Wide Analysis, Identification, and Characterization of the PFK gene Family Members of Populus deltoides

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FORESTS 2023年第6期14卷 1104页

作者： Kim, Tae-Lim Denison, Michael Immanuel Jesse Lim, Hyemin Chung, Hoyong Oh, Changyoung Natl Inst Forest Sci Dept Forest Bioresources Suwon 16631 South Korea 3BIGS Co Ltd Hwaseong 18469 South Korea

Plants produce two phosphorylated enzymes: pyrophosphate-dependent fructose-6-phosphate phosphotransferase (PFP) and ATP-dependent phosphofructokinase (PFK). However, the characterization of phosphofructokinase (PFK) family members and their functions remains unexplored in Eastern Cottonwood (Populus deltoides). This study comprehensively analyzed 17 phosphofructokinase genes in the P. deltoides genome, including their gene structure, conserved domains, evolutionary relationships, and expression patterns. Genome-wide analysis of the poplar genome identified 11 genes belonging to PdPFK and 6 genes belonging to PdPFP. Phylogenetic, gene structure, and motif analyses revealed two main categories of PFK genes: PFK with eleven genes and PFP with six genes. Quantitative PCR (qPCR) analysis demonstrated that all 17 phosphofructokinase genes were induced in various tissues and developmental stages of P. deltoides. PFPs were strongly expressed throughout P. deltoides' growth and development, notably in the roots, according to the transcript analysis. This genome-wide analysis provides insights into PFK and PFP genes and establishes a foundation for future investigations into the role of genes in P. deltoids.

关键词： PFK gene family characterization phylogenetic analysis gene expression pattern Populus deltoides

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Genome-wide identification and characterization of SPX domain-containing proteins and their responses to phosphorus and/or nitrogen deficiency in apple (Malus domestica Borkh.)

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SCIENTIA HORTICULTURAE 2023年 310卷

作者： Xie, Bin An, Xiuhong Chen, Yanhui Cheng, Cungang Zhang, Yanzhen Yang, An Li, Xin Kang, Guodong Zhou, Jiangtao Chinese Acad Agr Sci Res Inst Pomol Key Lab Mineral Nutr & Efficient Fertilizat Decidu Liaoning Prov Key Lab Fruit Germplasm Resources Ut Xingcheng 125100 Liaoning Peoples R China Hebei Agr Univ Mountainous Areas Res Inst Natl Engn Res Ctr Agr Northern Mountainous Areas Baoding 071001 Peoples R China

Phosphate (Pi) and nitrate (Ni) deficiencies are the major constraints for apple, significantly impacting the fruit yield and quality. SPX domain-containing proteins play important roles in Pi homeostasis and Pi signaling pathway, as well as phosphorus (P) and nitrogen (N) nutrient balance in plants. However, SPX domain -containing proteins have not been analysed systematically in apple (Malus domestica Borkh.). In this study, a total of 25 MdSPXs were identified in M. domestica. According to phylogenetic relationships and C-terminal domains analyses, these MdSPXs were divided into four subfamilies: SPX (6), SPX-EXS (12), SPX-MFS (3), and SPX-RING (4).16 MdSPXs from these four subfamiles contain Phosphate starvation response 1-binding site (P1BS) in the promoter region. The expression profiles showed that 15 MdSPXs responded to P deficiency as well as N and P deficiencies, respectively, 13 MdSPXs responded to N deficiency in hydroponic 'M26' seedlings. The response of 11 common differentially expressed genes exhibited apparent tissue-specificity. Pi starvation response (PSR) involved in MdSPX1, MdSPX2, and MdSPX5 depended on the N availability. In the predicted protein-protein interaction network, MdSPXs interact with important components of the P signaling network such as MdPHO2, MdPHRs, MdSIZ1, and MdPHTs, etc., and components of the N signaling network such as with MdNRTs and MdNLP6, etc., in addition to several TFs such as bZIP TF MdTGA4, an important Ni-responsive TF. Overexpression of MdTGA4 changed the expression of MdSPX2, MdSPX4, MdPHO1;6, MdPHO1;7, MdPHO1;9 and MdMFS2 in the transgenic apple calluses, and these genes contain as-1, a special binding element of TGA, in the promoter region. These results preliminarily confirmed the key role of MdSPXs in Pi homeostasis and Pi signaling pathway as well as Ni signaling pathway, offered a valuable guide for integrating N-P interactive regulation pathways in apple, and provided important genetic resources for the

关键词： SPX Apple Phosphorus and nitrogen deficiencies gene expression pattern Interaction network

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De novo genes in Arachis hypogaea cv. Tifrunner: systematic identification, molecular evolution, and potential contributions to cultivated peanut

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PLANT JOURNAL 2022年第4期111卷 1081-1095页

作者： Song, Hui Guo, Zhonglong Zhang, Xiaojun Sui, Jiongming Qingdao Agr Univ Coll Grassland Sci Grassland Agrihusb Res Ctr Qingdao Peoples R China Peking Univ State Key Lab Prot & Plant Gene Res Peking Tsinghua Ctr Life Sci Sch Life Sci Beijing Peoples R China Peking Univ Sch Adv Agr Sci Beijing Peoples R China Qingdao Agr Univ Coll Agron Qingdao Peoples R China

De novo genes are derived from non-coding sequences, and they can play essential roles in organisms. Cultivated peanut (Arachis hypogaea) is a major oil and protein crop derived from a cross between Arachis duranensis and Arachis ipaensis. However, few de novo genes have been documented in Arachis. Here, we identified 381 de novo genes in A. hypogaea cv. Tifrunner based on comparison with five closely related Arachis species. There are distinct differences in gene expression patterns and gene structures between conserved and de novo genes. The identified de novo genes originated from ancestral sequence regions associated with metabolic and biosynthetic processes, and they were subsequently integrated into existing regulatory networks. De novo paralogs and homoeologs were identified in A. hypogaea cv. Tifrunner. De novo paralogs and homoeologs with conserved expression have mismatching cis-acting elements under normal growth conditions. De novo genes potentially have pluripotent functions in responses to biotic stresses as well as in growth and development based on quantitative trait locus data. This work provides a foundation for future research examining gene birth processes and gene function in Arachis and related taxa.

关键词： Arachis hypogaea cv Tifrunner de novo gene homoeolog gene expression pattern paralog quantitative trait loci selective pressure

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Evaluation of growth and gene expression patterns of different strains related to SMD utilization in largemouth bass

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AQUACULTURE 2020年 523卷 735214-000页

作者： Yi, Shao-Kui Wang, Han-Ping Xie, Peng Li, Xiao-Xia Yao, Hong Ohio State Univ Ohio Ctr Aquaculture Res & Dev South Ctr Piketon OH 45661 USA

In order to evaluate utilization variation of, and growth response to, soybean meal diets in largemouth bass for breeding programs, WA diets with 0%, 35%, and 50% fishmeal having been replaced by soybean meal (SBM;S0, S35, and S50) were administered to three northern strains of largemouth bass (LMB;Jones, Marlow, and Remlinger). In the S35 and S50 groups, the Marlow and Remlinger strains had much better growth response to soybean meal diet (SMD) than the Jones strain, indicating that these two strains can better utilize diets containing high level of SMD. The results suggest that genetic variation exists for growth and feed efficiency in LMB fed diets containing both SBM and fishmeal, and genetic improvement may be possible for the trait of feed efficiency. In all the three strains, the growth performance of individuals in the S35 and S50 groups declined when compared with the S0 group, and the specific growth rate and weight gain correlated negatively with dietary levels of SBM. genes that were differentially expressed between dietary SBM levels were identified. Transcripts of genes related to insulin-signaling pathway and fatty acid metabolism and biosynthesis were significantly downregulated in the S35 and S50 groups compared with the S0 group. Interestingly, several transcripts of genes (egfr, pla2g4, and ugt) involved in GnRH-signaling pathway and steroid hormone biosynthesis were also downregulated in the S35 and S50 groups, inferring that feeding with SMD could impair the sexual development of juvenile LMB. Likewise, the genes related to insulin signaling and fatty acid metabolism and biosynthesis were downregulated in the Jones strain compared to the Marlow and Remlinger strains in all three treatments. Overall, the Marlow and Remlinger strains exhibited better adaptation to SBM diets, and could be considered as potential breeding candidates. These results also suggest that insulin-signaling pathway and fatty acid metabolism and biosynthesis play important r

关键词： Largemouth bass Soybean meal utilization Growth performance gene expression pattern

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