检索结果-内蒙古大学图书馆

Sugar nucleotide regeneration system for the synthesis of Bi- and triantennary N-glycans and exploring their activities against siglecs

引用

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 2022年 232卷 114146页

作者： Anwar, Mohammed Tarique Kawade, Sachin Kisan Huo, Yi-Ren Adak, Avijit K. Sridharan, Deepa Kuo, Yan-Ting Fan, Chen-Yo Wu, Hsin-Ru Lee, Yun-Sheng Angata, Takashi Lin, Chun-Cheng Natl Tsing Hua Univ Dept Chem Hsinchu 30013 Taiwan Acad Sinica Inst Biol Chem Taipei 11529 Taiwan Natl Tsing Hua Univ Instrumentat Ctr Hsinchu 30013 Taiwan Kaohsiung Med Univ Dept Med & Appl Chem Kaohsiung 80708 Taiwan

Enzymatic synthesis that is commenced by the sugar nucleotide regeneration system (SNRS) protocol can minimize 1) the consumption of exorbitant sugar nucleotides, 2) the amount of transferases required, and 3) byproduct feedback inhibition. In this study, LacNAc extensions/modifications of the N-linked mannose core were carried out efficiently with SNRS with high yields and purities on all branches in a uniform manner. In addition, we demonstrate that with SNRS, bacterial glycosyltransferases exhibit a wide acceptor tolerance for bi- and triantennary mannose core structures as substrates for target oligosaccharides. The synthesized small library of mannose core-based glycans and linear O-glycans were screened for their binding affinity against h-Siglecs 2, 4, 7, 9, 14, 15, and m-Siglec-15 to explore their structure-based binding preferences. microarray data revealed that each Siglec showed few distinct yet overlapping specificities. An increase in branching from mono to di or tri antennary did not necessarily lead to increasing affinity. glycans with the disialoside sequence alpha(2,3)alpha(2,8)/alpha(2,6)alpha(2,8) showed high specificity and affinity for Siglec-7, and sLex alpha(2,3) exhibited a strong affinity for Siglec-9. Explicit recognition of alpha(2,6) alpha(2,3)- linear and alpha(2,3)alpha(2,6)-branched glycans by Siglecs-2, 4, and 15, respectively, suggests that these structures can act as potential candidates for the further development of high-affinity ligands. (c) 2022 Elsevier Masson SAS. All rights reserved.

关键词： N-glycan Enzymatic synthesis Sialoside Siglec Sugar nucleotide regeneration glycan microarray

来源：评论

学校读者我要写书评

暂无评论

Simple and practical sialoglycan encoding system reveals vast diversity in nature and identifies a universal sialoglycan-recognizing probe derived from AB5 toxin B subunits

引用

GLYCOBIOLOGY 2022年第12期32卷 1101-1115页

作者： Sasmal, Aniruddha Khan, Naazneen Khedri, Zahra Kellman, Benjamin P. Srivastava, Saurabh Verhagen, Andrea Yu, Hai Bruntse, Anders Bech Diaz, Sandra Varki, Nissi Beddoe, Travis Paton, Adrienne W. Paton, James C. Chen, Xi Lewis, Nathan E. Varki, Ajit Univ Calif San Diego Glycobiol Res & Training Ctr La Jolla CA 92093 USA Univ Calif San Diego Dept Cellular & Mol Med La Jolla CA 92093 USA Univ Calif San Diego Dept Pediat La Jolla CA 92093 USA Univ Calif Davis Dept Chem Davis CA 95616 USA La Trobe Univ Dept Anim Plant & Soil Sci Bundoora Vic 3086 Australia La Trobe Univ Ctr AgriBiosci Bundoora Vic 3086 Australia Univ Adelaide Res Ctr Infect Dis Dept Mol & Biomed Sci Adelaide SA 5005 Australia Univ Calif San Diego Dept Med La Jolla CA 92093 USA

Vertebrate sialic acids (Sias) display much diversity in modifications, linkages, and underlying glycans. Slide microarrays allow high-throughput explorations of sialoglycan-protein interactions. A microarray presenting similar to 150 structurally defined sialyltrisaccharides with various Sias linkages and modifications still poses challenges in planning, data sorting, visualization, and analysis. To address these issues, we devised a simple 9-digit code for sialyltrisaccharides with terminal Sias and underlying two monosaccharides assigned from the nonreducing end, with 3 digits assigning a monosaccharide, its modifications, and linkage. Calculations based on the encoding system reveal >113,000 likely linear sialyltrisaccharides in nature. Notably, a biantennary N-glycan with 2 terminal sialyltrisaccharides could thus have >10(10) potential combinations and a triantennary N-glycan with 3 terminal sequences, >10(15) potential combinations. While all possibilities likely do not exist in nature, sialoglycans encode enormous diversity. While glycomic approaches are used to probe such diverse sialomes, naturally occurring bacterial AB(5) toxin B subunits are simpler tools to track the dynamic sialome in biological systems. Sialoglycan microarray was utilized to compare sialoglycan-recognizing bacterial toxin B subunits. Unlike the poor correlation between B subunits and species phylogeny, there is stronger correlation with Sia-epitope preferences. Further supporting this pattern, we report a B subunit (YenB) from Yersinia enterocolitica (broad host range) recognizing almost all sialoglycans in the microarray, including 4-O-acetylated-Sias not recognized by a Yersinia pestis orthologue (YpeB). Differential Sia-binding patterns were also observed with phylogenetically related B subunits from Escherichia coli (SubB), Salmonella Typhi (PltB), Salmonella Typhimurium (ArtB), extra-intestinal *** (EcPltB), Vibrio cholera (CtxB), and cholera family homologue of E. coli (EcxB

关键词： glycan microarray glycan coding bacterial toxin toxin B subunit Yersinia enterocolitica

来源：评论

学校读者我要写书评

暂无评论

SugarArray: A User-centred-designed Platform for the Analysis of Lectin and glycan microarrays 13

SugarArray: A User-centred-designed Platform for the Analysi...

引用

13th International Joint Conference on Biomedical Engineering Systems and Technologies

作者： Sucre, Aurora Pazos, Raquel Reichardt, Niels-Christian Garin-Muga, Alba Biodonostia eHlth Grp Donostia San Sebastian 20014 Spain Vicomtech eHlth & Biomed Applicat Donostia San Sebastian 20014 Spain Glycotechnol Lab CIC BiomaGUNE Paseo Miramon 182 San Sebastian 20014 Spain CIBER BBN Paseo Miramon 182 San Sebastian 20014 Spain

ISBN: (纸本)9789897583988

glycan and lectin microarrays are two arising technologies, very important to the glycomics field. Glycomics is the science that focuses on defining the structures and functions of carbohydrates in nature. These microarrays provide information regarding the interactions between specific carbohydrates and proteins, and it has many applications in clinical and research settings. Nevertheless, the availability of analytical software for these types of arrays is very limited, so researchers usually perform data processing and analytical pipelines manually, which is very time consuming and prone to error. SugarArray was born as a user-friendly and intuitive stand-alone solution that process the intensity data generated from glycan or lectin array studies, and displays the results to the user in an understandable manner The solution also allows the users to manage the data as needed, create data plots and automatically generate reports. This tool was intended to simplify the processing steps of the analytical pipeline, so the users can focus on what really matters: understanding the results.

关键词： Glycomics Lectin microarray glycan microarray microarray Data Analysis Data Visualization

来源：评论

学校读者我要写书评

暂无评论

Tumor cells express pauci- and oligomannosidic N-glycans in glycoproteins recognized by the mannose receptor (CD206)

引用

CELLULAR AND MOLECULAR LIFE SCIENCES 2021年第14期78卷 5569-5585页

作者： Stavenhagen, Kathrin Laan, Lisa C. Gao, Chao Mehta, Akul Y. Heimburg-Molinaro, Jamie Glickman, Jonathan N. van Die, Irma Cummings, Richard D. Harvard Med Sch Beth Israel Deaconess Med Ctr Dept Surg CLS 11087 3 Blackfan Circle Boston MA 02115 USA Vrije Univ Amsterdam Med Ctr Amsterdam UMC Dept Mol Cell Biol & Immunol Amsterdam Netherlands Harvard Med Sch Beth Israel Deaconess Med Ctr Dept Pathol Boston MA 02115 USA

The macrophage mannose receptor (CD206, MR) is an endocytic lectin receptor which plays an important role in homeostasis and innate immunity, however, the endogenous glycan and glycoprotein ligands recognized by its C-type lectin domains (CTLD) have not been well studied. Here we used the murine MR CTLD4-7 coupled to the Fc-portion of human IgG (MR-Fc) to investigate the MR glycan and glycoprotein recognition. We probed 16 different cancer and control tissues using the MR-Fc, and observed cell- and tissue-specific binding with varying intensity. All cancer tissues and several control tissues exhibited MR-Fc ligands, intracellular and/or surface-located. We further confirmed the presence of ligands on the surface of cancer cells by flow cytometry. To characterize the fine specificity of the MR for glycans, we screened a panel of glycan microarrays. Remarkably, the results indicate that the CTLD4-7 of the MR is highly selective for specific types of pauci- and oligomannose N-glycans among hundreds of glycans tested. As lung cancer tissue and the lung cancer cell line A549 showed intense MR-Fc binding, we further investigated the MR glycoprotein ligands in those cells by immunoprecipitation and glycoproteomic analysis. All enriched glycoproteins, of which 42 were identified, contained pauci- or oligomannose N-glycans, confirming the microarray results. Our study demonstrates that the MR CTLD4-7 is highly selective for pauci- and oligomannosidic N-glycans, structures that are often elevated in tumor cells, and suggest a potential role for the MR in tumor biology.

关键词： Mannose receptor glycans Cancer tissue Ligands glycan microarray Paucimannose Oligomannose Glycoproteomics C-type lectins glycan-binding proteins

来源：评论

学校读者我要写书评

暂无评论

Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-glycan Library for Functional Glycomics

引用

ADVANCED SCIENCE 2023年第30期10卷 e2303832页

作者： Ma, Wenjing Xu, Zhuojia Jiang, Yuhan Liu, Jialin Xu, Dandan Huang, Wei Li, Tiehai Chinese Acad Sci Shanghai Inst Mat Med State Key Lab Chem Biol Shanghai 201203 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Hangzhou Inst Adv Study Sch Pharmaceut Sci & Technol Hangzhou 310024 Peoples R China

N-Glycosylation, a main post-translational modification of Immunoglobulin G (IgG), plays a significant role in modulating the immune functions of IgG. However, the precise function elucidation of IgG N-glycosylation remains impeded due to the obstacles in obtaining comprehensive and well-defined N-glycans. Here, an easy-to-implement divergent approach is described to synthesize a 64-membered IgG N-glycan library covering all possible biantennary and bisected N-glycans by reprogramming biosynthetic assembly lines based on the inherent branch selectivity and substrate specificity of enzymes. The unique binding specificities of 64 N-glycans with different proteins are deciphered by glycan microarray technology. This unprecedented collection of synthetic IgG N-glycans can serve as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics to facilitate biomedical applications.

关键词： carbohydrates enzyme-catalyzed synthesis functional glycomics glycan microarray IgG N-glycan library

来源：评论

学校读者我要写书评

暂无评论

N-glycosylation of mannose receptor (CD206) regulates glycan binding by C-type lectin domains

引用

JOURNAL OF BIOLOGICAL CHEMISTRY 2022年第12期298卷 102591页

作者： Stavenhagen, Kathrin Mehta, Akul Y. Laan, Lisa Gao, Chao Heimburg-Molinaro, Jamie van Die, Irma Cummings, Richard D. Harvard Med Sch Beth Israel Deaconess Med Ctr Dept Surg Boston MA 02115 USA Amsterdam UMC VU Med Ctr Dept Mol Cell Biol & Immunol Amsterdam Netherlands

The macrophage mannose receptor (MR, CD206) is a transmembrane endocytic lectin receptor, expressed in selected immune and endothelial cells, and is involved in immunity and maintaining homeostasis. Eight of the ten extracellular do-mains of the MR are C-type lectin domains (CTLDs) which mediate the binding of mannose, fucose, and GlcNAc in a calcium-dependent manner. Previous studies indicated that self-glycosylation of MR regulates its glycan binding. To further explore this structure-function relationship, we studied herein a recombinant version of mouse MR CTLD4-7 fused to human Fc-portion of IgG (MR-Fc). The construct was expressed in different glycosylation-mutant cell lines to study the influence of differential glycosylation on receptor glycan-binding properties. We conducted site-specific N-and O-glycosylation analysis and glycosylation site characterization using mass spectrometry by which several novel O-glycosyla-tion sites were identified in mouse MR and confirmed in human full-length MR. This information guided experiments evaluating the receptor functionality by glycan microarray analysis in combination with glycan-modifying enzymes. Treatment of active MR-Fc with combinations of exoglycosi-dases, including neuraminidase and galactosidases, resulted in the loss of trans-binding (binding of MR CTLDs to non-MR glycans), due to unmasking of terminal, nonreducing GlcNAc in N-glycans of the MR CTLDs. Regalactosylation of N-glycans rescues mannose binding by MR-Fc. Our results indicate that glycans within the MR CTLDs act as a regulatory switch by masking and unmasking self-ligands, including terminal, nonreducing GlcNAc in N-glycans, which could control MR activity in a tissue-and cell-specific manner or which poten-tially affect bacterial pathogenesis in an immunomodulatory fashion.

关键词： mannose receptor glycosylation glycobiology mass spectrometry lectin N-linked glycosylation O-linked glycosylation glycan microarray glycoproteomics

来源：评论

学校读者我要写书评

暂无评论

Binding of the SARS-CoV-2 spike protein to glycans

引用

Science Bulletin 2021年第12期66卷 1205-1214,M0004页

作者： Wei Hao Bo Ma Ziheng Li Xiaoyu Wang Xiaopan Gao Yaohao Li Bo Qin Shiying Shang Sheng Cui Zhongping Tan NHC Key Laboratory of Systems Biology of Pathogens Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Chemistry and Biochemistry and BioFrontiers Institute University of ColoradoBoulder CO 80303USA School of Pharmaceutical Sciences Tsinghua UniversityBeijing W0084China

The pandemic of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a high number of deaths in the *** combat it,it is necessary to develop a better understanding of how the virus infects host *** normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate(HS)and sialic acid-containing glycolipids/*** this study,we examined and compared the binding of the subunits and spike(S)proteins of SARS-CoV-2,SARS-Co V,and Middle East respiratory disease(MERS)-Co V to these *** results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was ***,this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells,and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses.

关键词： SARS-CoV-2 S protein Heparan sulfate glycan microarray Surface plasmon resonance Sulfation Sialic acid

来源：评论

学校读者我要写书评

暂无评论

alpha 2,3-Linked Sialic Acids Are the Potential Attachment Receptor for Shaan Virus Infection in MARC-145 Cells

引用

MICROBIOLOGY SPECTRUM 2022年第4期10卷 e0125622页

作者： Jang, Seong Sik Noh, Ji Yeong Kim, Min Chan Lim, Hyun A. Song, Min Suk Kim, Hye Kwon Chungbuk Natl Univ Coll Nat Sci Dept Biol Sci & Biotechnol Cheongju South Korea Chungbuk Natl Univ Coll Med Dept Microbiol Cheongju South Korea Chungbuk Natl Univ Med Res Inst Cheongju South Korea

Shaan virus (ShaV), a novel species of the genus Jeilongvirus, family Paramyxoviridae, was isolated from an insectivore bat (Miniopterus schreibersii) in Korea in 2016. ShaV particles contain a hemagglutinin-neuraminidase (HN) glycoprotein in their envelope that allows the virus to target cells. Typically, diverse paramyxoviruses with HN glycoprotein are reported to interact predominantly with sialic acids, but there are no studies of receptors for ShaV. In this study, the identification of potential receptors for ShaV was demonstrated using sialidase treatments, glycan microarray, magnetic bead-based virus binding assay, and neuraminidase inhibitor treatments. Pretreatment of MARC-145 cells with sialidase, which cleaves alpha 2,3-linked sialic acids, showed higher inhibition of viral infection than alpha 2,6-linked-specific sialidase. These data were supported by the binding of ShaV to predominantly alpha 2,3-linked sialylated glycans in the screening of sialyl linkage patterns through glycan microarray. To further confirm the direct interaction between ShaV and alpha 2,3-linked sialic acids, ShaV was incubated with alpha 2,3-or alpha 2,6-linked sialylated glycans conjugated to magnetic beads, and binding signals were detected only for alpha 2,3-linked sialylated glycans. In addition, the potential of sialic acids as a receptor was demonstrated by the viral replication inhibitory effect of the neuraminidase inhibitor 2,3-dehydro-2-deoxy-N-acetylneuraminicacid (DANA) in the mature virion release steps. Collectively, these results support that alpha 2,3-linked sialic acids are the potential receptor for ShaV infection in MARC-145 cells. IMPORTANCE Bats host major mammalian paramyxoviruses, and novel paramyxoviruses are increasingly being reported around the world. Shaan virus (ShaV), from the genus Jeilongvirus, family Paramyxoviridae, has a potential attachment glycoprotein, HN. Here, we identify that ShaV binds to sialic acid and demonstrate that alpha 2,3-linked s

关键词： Shaan virus bat paramyxovirus glycan microarray magnetic bead neuraminidase receptor sialic acid

来源：评论

学校读者我要写书评

暂无评论

The 3-O-sulfation of heparan sulfate modulates protein binding and lyase degradation

引用

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2021年第3期118卷 e2012935118页

作者： Chopra, Pradeep Joshi, Apoorva Wu, Jiandong Lu, Weigang Yadavalli, Tejabhiram Wolfert, Margreet A. Shukla, Deepak Zaia, Joseph Boons, Geert-Jan Univ Georgia Complex Carbohydrate Res Ctr Athens GA 30602 USA Univ Georgia Dept Chem Athens GA 30602 USA Boston Univ Med Ctr Biomed Mass Spectrometry Dept Biochem Boston MA 02118 USA Univ Illinois Dept Ophthalmol & Visual Sci Chicago IL 60612 USA Univ Utrecht Utrecht Inst Pharmaceut Sci Dept Chem Biol & Drug Discovery NL-3584 CG Utrecht Netherlands Univ Utrecht Bijvoet Ctr Biomol Res NL-3584 CG Utrecht Netherlands Univ Illinois Dept Microbiol & Immunol Chicago IL 60612 USA

Humans express seven heparan sulfate (HS) 3-O-sulfotransferases that differ in substrate specificity and tissue expression. Although genetic studies have indicated that 3-O-sulfated HS modulates many biological processes, ligand requirements for proteins engaging with HS modified by 3-O-sulfate (3-OS) have been difficult to determine. In particular, the context in which the 3-OS group needs to be presented for binding is largely unknown. We describe herein a modular synthetic approach that can provide structurally diverse HS oligosaccharides with and without 3-OS. The methodology was employed to prepare 27 hexasaccharides that were printed as a glycan microarray to examine ligand requirements of a wide range of HS-binding proteins. The binding selectivity of antithrombin-III (AT-III) compared well with anti-Factor Xa activity supporting robustness of the array technology. Many of the other examined HS-binding proteins required an IdoA2S-GlcNS3S6S sequon for binding but exhibited variable dependence for the 2-OS and 6-OS moieties, and a GlcA or IdoA2S residue neighboring the central GlcNS3S. The HS oligosaccharides were also examined as inhibitors of cell entry by herpes simplex virus type 1, which, surprisingly, showed a lack of dependence of 3-OS, indicating that, instead of glycoprotein D (gD), they competitively bind to gB and gC. The compounds were also used to examine substrate specificities of heparin lyases, which are enzymes used for depolymerization of HS/heparin for sequence determination and production of therapeutic heparins. It was found that cleavage by lyase II is influenced by 3-OS, while digestion by lyase I is only affected by 2-OS. Lyase III exhibited sensitivity to both 3-OS and 2-OS.

关键词： 3-O-sulfation glycan microarray anti-Factor Xa herpes simplex virus 1 heparin lyases

来源：评论

学校读者我要写书评

暂无评论

Biomimetic Glyconanoparticle Vaccine for Cancer Immunotherapy

引用

ACS NANO 2019年第3期13卷 2936-2947页

作者： Reuven, Eliran Moshe Ben-Arye, Shani Leviatan Yu, Hai Duchi, Roberto Perota, Andrea Conchon, Sophie Abramovitch, Shirley Bachar Soulillou, Jean-Paul Galli, Cesare Chen, Xi Padler-Karavani, Vered Tel Aviv Univ George S Wise Fac Life Sci Dept Cell Res & Immunol IL-69978 Tel Aviv Israel Univ Calif Davis Dept Chem Davis CA 95616 USA Avantea Lab Reprod Technol Via Porcellasco 7-F I-26100 Cremona Italy CHU Nantes Inst Transplantat Urol Nephrol INSERM UMR 1064 F-44000 Nantes France Fdn Avamtea Cremona Via Cabrini 12 I-26100 Cremona Italy

Cancer immunotherapy aims to harness the immune system to combat malignant processes. Transformed cells harbor diverse modifications that lead to formation of neoantigens, including aberrantly expressed cell surface carbohydrates. Targeting tumor-associated carbohydrate antigens (TACA) hold great potential for cancer immunotherapy. N-glycolylneuraminic acid (Neu5Gc) is a dietary non-human immunogenic carbohy-drate that accumulates on human cancer cells, thereby generating neoantigens. In mice, passive immunotherapy with anti-Neu5Gc antibodies inhibits growth of Neu5Gc-positive tumors. Here, we designed an active cancer vaccine immunotherapy strategy to target Neu5Gc-positive tumors. We generated biomimetic glyconanoparticles using engineered alpha Gal knockout porcine red blood cells to form nanoghosts (NGs) that either express (NG(Pos)) or lack expression (NG(neg)) of Neu5Gc-glycoconjugates in their natural context. We demonstrated that optimized immunization of "human-like" Neu5Gc-deficient Cmah(-/-) mice with NG(Pos) glyconanoparticles induce a strong, diverse and persistent anti-Neu5Gc IgG immune response. The resulting anti-Neu5Gc IgG antibodies were also detected within Neu5Gc-positive tumors and inhibited tumor growth in vivo. Using detailed glycan microarray analysis, we further demonstrate that the kinetics and quality of the immune responses influence the efficacy of the vaccine. These findings reinforce the potential of TACA neoantigens and the dietary non-human sialic acid Neu5Gc, in particular, as immunotherapy targets.

关键词： neoantigen cancer immunotherapy biomimetic glyconanoparticle glycan microarray sialic acid N-glycolylneuraminic acid

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：