检索结果-内蒙古大学图书馆

high performance liquid chromatography - Fourier Transform Infrared Spectroscopy Coupling: A Comprehensive Review

CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY 2024年 1-12页

作者： Halko, Radoslav Pavelek, Denis Kaykhaii, Massoud Comenius Univ Fac Nat Sci Dept Analyt Chem Ilkovicova 6 Bratislava 84215 Slovakia Univ Tasmania Coll Sci & Engn Sch Nat Sci Chem Hobart Australia

This review presents a critical examination of the interface for coupling high performance liquid chromatography (HPLC) with Fourier transform infrared spectrometry (FTIR) since 2010. This coupling offers a robust analytical approach characterized by exceptional chemical specificity and the capacity to analyze complex multi-component mixtures qualitatively and quantitatively with high sensitivity, particularly in low limit of detection ranges. This coupling enables the identification of individual components of a mixture by IR after their separation by HPLC, although challenges arise from the potential distortion of infrared spectra by mobile phase components. Addressing this issue necessitates the implementation of suitable interfaces, such as flow cells or off-line indirect measurement methods like hot inert gas streams or ultrasonic nebulizers. The key parameters influencing the coupling of HPLC-FTIR include the solvent elimination methods, mode of FTIR technique, and IR background for accurate analyte identification. Moreover, the composition of the mobile phase and the utilization of buffer solutions in the HPLC mobile phase profoundly impact analyte identification by FTIR.

关键词： Fourier transform infrared spectrometry high performance liquid chromatography coupling interface flow cell solvent elimination

来源：评论

学校读者我要写书评

暂无评论

high performance liquid chromatography-tandem mass spectrometry determination of patulin and ochratoxin a in commercial fruit juices after their extraction with a green synthesized metal organic framework-based dispersive micro solid phase extraction procedure

引用

MICROCHEMICAL JOURNAL 2022年 179卷

作者： Mohebbi, Ali Nemati, Mahboob Farajzadeh, Mir Ali Mogaddam, Mohammad Reza Afshar Lotfipour, Farzaneh Tabriz Univ Med Sci Food & Drug Safety Res Ctr Tabriz Iran Univ Tabriz Fac Chem Dept Analyt Chem Tabriz Iran Near East Univ Engn Fac North CyprusMersin 10 TR-99138 Nicosia Turkey Tabriz Univ Med Sci Pharmaceut Anal Res Ctr Tabriz Iran Univ Ottawa Fac Med Dept Biochem Microbiol & Immunol Ottawa ON K1H 8M5 Canada

Herein, for the first time, a vitamin-based metal organic framework is synthesized from vitamin B3 (as a bio linker) and cobalt ions (as a metallic center) and employed as an efficient sorbent in dispersive micro solid phase extraction of patulin and ochratoxin A from fruit juice samples. The extracted analytes are quantitatively analyzed using high performance liquid chromatography-tandem mass spectrometry. In this work, after synthesizing the sorbent, it was characterized using X-ray diffraction, Fourier transform infrared spectrophotometry, scanning electron microscopy, nitrogen adsorption/desorption, and energy-dispersive X-ray spectroscopy. In the following, it was used as an efficient sorbent in the offered microextraction method and numerous parameters that could affect extraction efficiency of the method were investigated and optimized using response surface methodology. Based on the method validation results, limits of detection and quantification were 39.6 and 131.7 ng L-1 for patulin, and 24.8 and 82.6 ng L-1 for ochratoxin A, respectively. The extraction recoveries for patulin and ochratoxin A were 75 and 87%, respectively. Repeatability of the suggested approach, expressed as relative standard deviations, were equal to or<5% (at a concentration of 250 ng L-1 of each analyte). Also, environmentally friendly synthesis of the metal organic framework (using vitamin B3 as a linker, water as the reaction solvent, and mild conditions) and usage low amount or volumes of the sorbent and organic solvents in the microextraction procedure were other advantages of the current work.

关键词： Central composite design Dispersive micro solid phase extraction high performance liquid chromatography Metal organic framework Fruit juice

来源：评论

学校读者我要写书评

暂无评论

high performance liquid chromatography Analytical Method Development for Reduced Sulfur Species Quantification in Cyanide Leach Solutions 9

High Performance Liquid Chromatography Analytical Method Dev...

引用

9th International Symposium on Hydrometallurgy 2023

作者： Duru, N. Nesbitt, C. University of Nevada Reno United States

ISBN: (纸本)9780873355216

Development of an accurate analytical method for sulfur species in cyanide leaching units of gold ores where sulfur speciation occurs has important role to explain the metal recovery deviations. This study presents the development steps of high-performance liquid chromatography (HPLC) method to analyze reduced sulfur species thiocyanate (SCN-), thiosulfate (S2O32- ), trithionate (S3O62- ), tetrathionate (S4O62-) in cyanide leaching circuits for gold ores. HPLC method developed has resulted in precise quantification of target sulfur species in leach solutions. Method development and validation results were illustrated on analytical graphs contains each species’ peak number, retention time (min), type, width (min), area (mAU*s), height (mAU), area distribution (%) of the peak. Copyright © 2023 Society for Mining, Metallurgy & Exploration. All Rights Reserved.

关键词： high performance liquid chromatography

来源：评论

学校读者我要写书评

暂无评论

Development of chiral and achiral high performance liquid chromatography methods for the analysis of main flavour compound of hazelnut

引用

EUROPEAN FOOD RESEARCH AND TECHNOLOGY 2025年第6期251卷 1319-1329页

作者： Vybohova, Veronika Hrobonova, Katarina Puchl'ova, Eva Slovak Univ Technol Bratislava Inst Analyt Chem Fac Chem & Food Technol Radlinskeho 9 Bratislava 81237 Slovakia Axxence Slovakia SRO Mickiewiczova 9 Bratislava 81107 Slovakia

(E)-5-Methylhept-2-en-4-one is a key flavour compound in hazelnuts with practical applications in perfumery, flavour, and food industries due to its unique sensory properties. This study developed an effective and sustainable achiral reverse-phase high-performance liquid chromatography (HPLC) method with diode array detection (DAD) to determine (E)-5-methylhept-2-en-4-one and its reaction intermediates prepared via chiral-pool-based chemoenzymatic synthesis. Given that two reaction intermediates are thermally unstable at the high temperatures used in gas chromatography, HPLC separation at ambient temperatures (avg. 25 degrees C) was an appropriate approach. Optimised HPLC conditions consisting of C18 type stationary phase, mixture of acetonitrile: water (60:40, v/v) as mobile phase under isocratic conditions and flow rate 0.5 ***-1 were used. Chiral normal phase HPLC-DAD method with polysaccharide derivative stationary phase and n-hexane:propan-2-ol (90:10, v/v) as mobile phase was used for separation of enantiomers of (E)-5-methylhept-2-en-4-one. Both achiral and chiral methods were successfully used for monitoring the course of chemoenzymatic synthesis, conversion of reaction intermediates and purity of final product. The achiral HPLC-DAD method showed good linearity (R2 = 0.9997) in concentration range 0.5-100 mu ***-1. The limits of detection and quantitation were 0.1 and 0.3 mu ***-1, respectively. Using developed chiral HPLC-DAD method, sufficient enantioseparation was achieved with resolution of 4.73. Values of LOD and LOQ were 0.2 mu ***-1 and 0.5 mu ***-1, respectively. Besides synthesis monitoring and purity product determination, the developed chiral and achiral HPLC methods could find application in the analysis of (S)-(E)-5-methylhept-2-en-4-one in nut-flavoured foods (e.g., pastries, cookies, desserts, beverages) and personal care products, as well as differentiate pure olive oil from those adulterated with hazelnut oil.

关键词： (E)-5-Methylhept-2-en-4-one high performance liquid chromatography Reaction intermediates Enantiomers Hazelnut flavour

来源：评论

学校读者我要写书评

暂无评论

Ionic liquid Dispersion liquid liquid Microextraction Phthalates From Sewage Coupled With high performance liquid chromatography Detection

引用

WATER AIR AND SOIL POLLUTION 2025年第5期236卷 1-10页

作者： Chen, Xiaojing Qiao, Fengxia Chen, Huizhen Baoding Univ Coll Biochem & Environm Engn Baoding 071002 Peoples R China Huapu Shijiazhuang Pharmaceut Co Ltd Shijiazhuang 050035 Peoples R China

The study presents a green and efficient approach for the determination of phthalates, such as diethyl phthalate (DEP) and dibutyl phthalate (DBP), in sewage water. The method combines dispersive liquid-liquid microextraction (DLLME) with high-performance liquid chromatography (HPLC) detection, utilizing ionic liquids, specifically [C6MIM]PF6, as environmentally friendly extraction solvents, and methanol as the dispersant. Through optimization of parameters, including extractive solvent volume and kind, dispersive solvent selection, and salt influence, the method demonstrates exceptional sensitivity and selectivity. Validation studies confirm its analytical robustness, with low limits of detection, high precision, and successful avoidance of matrix interferences. Application to real industry sewage samples from one sewage outfall reveals elevated DEP and DBP detection levels, highlighting the method's efficacy in monitoring environmental pollution. Good linearity of DEP and DBP in the range of 0.200-180.00 mu g/mL (R-2: 0.9999) could be obtained. The spiked recoveries of DEP and DBP were in a range of 90.23%-95.11% and 91.18%-107.4% (RSD <= 5.34) with the detection limits of 0.0053 mu g/mL and 0.0094 mu g/mL respectively. The choice of ionic liquids aligns with green analytical chemistry principles, contributing to sustainable and eco-friendly analytical methodologies. In conclusion, the developed DLLME-HPLC method stands as a promising tool for rapid, efficient, and environmentally conscious determination of trace-level phthalates in complex environmental matrices.

关键词： Ionic liquid Dispersive liquid-liquid microextraction high performance liquid chromatography Phthalates Sewage

来源：评论

学校读者我要写书评

暂无评论

Magnetic Carbon as an Adsorbent for the Enrichment of Carbamate Pesticides in Magnetic Solid Phase Extraction Prior to high performance liquid chromatography

引用

JOURNAL OF CHROMATOGRAPHIC SCIENCE 2025年第5期63卷 bmaf030页

作者： Homchun, Poonsiri Gamonchuang, Jirasak Burakham, Rodjana Khon Kaen Univ Fac Sci Mat Chem Res Ctr Dept Chem Khon Kaen 40002 Thailand

A simple one-step synthesis method was proposed to prepare magnetic carbon material, which were subsequently employed for the magnetic solid phase extraction of carbamate pesticides prior to their determination by high performance liquid chromatography. The optimized condition resulted in the efficient extraction of a 30-mL sample solution using 50 mg of magnetic carbon as a sorbent, a 2-min vortex loading time and 1000 mu L of acetone as an elution solvent with a 3-min vortex elution. The linear range of the proposed method was 5-200 mu g L-1, with limits of detection and quantitation in the ranges of 3-40 and 5-60 mu g L-1, respectively. The relative standard deviations were <9.5%, which indicated a high level of precision. The results suggested that the proposed magnetic solid phase extraction method was an effective enrichment procedure and could be effectively implemented for the determination of carbamate pesticides in fruit samples. Satisfactory recoveries in the range of 70.2%-120.0% were obtained with the relative standard deviations of <11.6%. The method provided the benefit of a rapid extraction process, and the magnetic carbon functioned as an eco-friendly adsorbent that could be prepared in an easy manner.

关键词： high performance liquid chromatography

来源：评论

学校读者我要写书评

暂无评论

Species-specific Antimony Isotope Analysis by high performance liquid chromatography Coupled with Multicollector ICPMS Using Hydride Generation as an Interface

引用

ANALYTICAL CHEMISTRY 2025年第4期97卷 2264-2272页

作者： Chen, Linjie Du, Yuanyuan Li, Shuyang Liu, Xing Shen, Jun Zhu, Zhenli China Univ Geosci State Key Lab Biogeol & Environm Geol Wuhan 430078 Peoples R China China Univ Geosci Fac Mat Sci & Chem Wuhan 430078 Peoples R China Hubei Key Lab Yangtze Catchment Environm Aquat Sci Wuhan 430078 Peoples R China

A novel method has been developed for the simultaneous online determination of the isotopic compositions of different antimony (Sb) species in a single analytical run using high-performance liquid chromatography (HPLC) coupled with multicollector inductively coupled plasma mass spectrometry (MC-ICPMS), with hydride generation (HG) serving as the interface. Various parameters affecting the precision of Sb isotope analysis including HG conditions, transient signal processing methods and peak integration windows, were optimized. The linear regression slope method and a 100% peak integration window provided the optimal precision. Under optimized conditions, our method achieved external 2SD precisions better than 0.05 parts per thousand for both Sb(III) and Sb(V), with minimal consumption of 0.5 ng for Sb(III) and 5 ng for Sb(V). Furthermore, flow injection (FI) coupled with HG-MC-ICPMS demonstrated precise Sb isotopic analysis with sample requirements as low as 0.25 ng. The proposed methods were validated by analyzing delta 123Sb in synthetic solutions and reference materials. Additionally, it was applied to investigate isotopic fractionation during the reduction of Sb(V) by KI, revealing preferential reduction of the light Sb isotope(121Sb). The isotopic compositions of Sb(V) varied from -0.04- 1.18 parts per thousand, fitting well with a Rayleigh fractionation model and yielding a fractionation factor (alpha Sb(III)-Sb(V)) of 0.99831. In summary, this approach enables high precision isotopic analysis of Sb(III) and Sb(V) simultaneously with reduced sample consumption, providing a powerful tool for investigating Sb isotopic fractionation in various environmental processes and advancing our understanding of the Sb biogeochemical cycle.

关键词： high performance liquid chromatography

来源：评论

学校读者我要写书评

暂无评论

Preconcentration and purification of oligonucleotides from heat-treated human plasma by anion-exchange microextraction devices coupled to high performance liquid chromatography-mass spectrometry

引用

ANALYTICA CHIMICA ACTA 2025年 1342卷 343624页

作者： Eitzmann, Derek R. Anderson, Jared L. Iowa State Univ Dept Chem 1605 Gilman Hall Ames IA 50011 USA

Background: Methodologies that preconcentrate high quality nucleic acids (NAs) for downstream assays are essential for their accurate and reproducible analysis by mass spectrometry (MS). Established methods rely on solid-phase extraction that involves numerous steps and user intervention or liquid-liquid extractions that are time-consuming and employ toxic organic solvents. A promising alternative methodology involves anion- exchange microextraction sorbents that selectively isolate NAs through electrostatic interactions with the negatively charged phosphodiester backbone. The microextraction devices recover and preconcentrate NAs using a salt-containing solution, which is generally incompatible with MS analysis. Results: Six anion-exchange microextraction sorbents featuring monomers derived from 2-aminoethyl methacrylate were synthesized and examined in this study to understand the interactions that take place between NAs and the ammonium cationic moiety. Sorbent affinities for bovine serum albumin, small single-stranded oligonucleotides, RNA, short double-stranded DNA), and 2000 bp dsDNA were determined. Recoveries of an oligonucleotide and 2000 bp dsDNA were measured and examined in salt solutions of varied concentration, anion species, and organic additives. high oligonucleotide preconcentration factors of 8.6 +/- 0.2 were obtained for the sorbent featuring two cationic ammonium moieties on each monomer using 500.0 mM ammonium perchlorate. A separate sorbent composed of dimethyl ethyl ammonium moieties produced a preconcentration factor of 4.6 +/- 0.2 using only 31.25 mM ammonium perchlorate. The sorbents were demonstrated in the complete workflow in which an analog antisense oligonucleotide was spiked into human plasma and purified, enabling successful molecular weight analysis. Significance: This study demonstrates the compatibility of microextraction recovery solutions comprised of ammonium perchlorate with reversed-phase and hydrophilic interaction chromatog

关键词： Nucleic acids Sample preparation Mass spectrometry Oligonucleotides high performance liquid chromatography

来源：评论

学校读者我要写书评

暂无评论

Model-based design of experiments for efficient and accurate isotherm model identification in high performance liquid chromatography

引用

COMPUTERS & CHEMICAL ENGINEERING 2025年 195卷

作者： Katsoulas, Konstantinos Galvanin, Federico Mazzei, Luca Besenhard, Maximilian Sorensen, Eva UCL Sargent Ctr Proc Syst Engn Dept Chem Engn Torrington Pl London WC1E 7JE England

chromatography is a key purification process in the pharmaceutical industry. The process design is based on knowledge of the adsorption isotherm that describes the separation within the chromatographic column. Although obtaining the values of isotherm model parameters has traditionally been the work of experimentalists, recently design methods based on mathematical models have emerged, and for these, accurate isotherm models and model parameter values are crucial. Different methods exist for parameter estimation, all depending on experiment execution. Model-Based Design of Experiments (MBDoE) can be used to optimally design experiments that maximise the information obtained from each experiment. In this work, we propose an MBDoE-based methodology that aims to identify the most suitable isotherm model, to estimate its parameters, and to evaluate its predictive capability. The methodology is tested on an in-silico case study where the performance is compared to that of traditional factorial design of experiments.

关键词： Modelling high performance liquid chromatography Model-Based Design of Experiments Parameter estimation Isotherm identification

来源：评论

学校读者我要写书评

暂无评论

high performance liquid chromatography coupled with post - Column derivatization methods in food analysis: Chemistries and applications in the last two decades

引用

FOOD CHEMISTRY 2024年 443卷 138577页

作者： Tsiasioti, Apostolia Tzanavaras, Paraskevas D. Aristotle Univ Thessaloniki Fac Sci Sch Chem Lab Analyt Chem GR-54124 Thessaloniki Greece

high performance liquid chromatography coupled with post-column derivatization is used for increasing the sensitivity and selectivity of the desirable analytes after the chromatographic separation. The transformation of the analytes can be conducted through the addition of a suitable reagent in the eluted stream or the ultraviolet irradiation of the eluted analytes, forming detectable derivatives for ultraviolet or fluorescence detectors. This review focuses on the developed methods using high performance liquid chromatography coupled with postcolumn derivatization for the determination of substances in food samples during the last two decades. The significance of the determination of each analyte in foods and the existing guidelines in each case are discussed. Preparation of the samples and the analytical methods are commented. For each analyte, official methods and commercially available systems and reagents are mentioned, as well.

关键词： Post-column derivatization high performance liquid chromatography Chemical derivatization Photochemical derivatization Food samples UV-Vis FLD

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：