Numerous plants were tested for ihibitors of human hageman factor fragment (HFf), plasma kallikrein, urinary kallikrein, plasmin, thrombin, porcine pancreatic kallikrein and bovine factor Xa, trypsin, and chymotrypsin...
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Numerous plants were tested for ihibitors of human hageman factor fragment (HFf), plasma kallikrein, urinary kallikrein, plasmin, thrombin, porcine pancreatic kallikrein and bovine factor Xa, trypsin, and chymotrypsin. Pumpkin seeds and iris bulbs contain trypsin inhibitors which specifically inhibit HFf. Flower *** those of tulip, lily, hyacinth, and *** unrecognized rich sources of inhibitors with different inhibitory spectrums and physicochemical properties.
hageman factor fragment (HFf) was isolated from acetone-treated human plasma. The Km and kcat [catalytic constant] of HFf were determined with a natural substrate, plasma prekallikrein, and 34 synthetic amides and est...
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hageman factor fragment (HFf) was isolated from acetone-treated human plasma. The Km and kcat [catalytic constant] of HFf were determined with a natural substrate, plasma prekallikrein, and 34 synthetic amides and esters. The highest catalytic efficiency, kcat/Km, was found with prekallikrein (2980 mM-1). Of the synthetic substrates, D-Pro-Phe-Arg-pNA, Ac-Phe-Arg-OEt, Cbz-Arg-OMe and Bz-Ile-Glu-Gly-Arg-pNA had the 4 largest kcat/Km values (130-670 mM-1s-1).
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