检索结果-内蒙古大学图书馆

Real-time evaluation of aggregation using confocal imaging and image analysis tools

ANALYST 2014年第3期139卷 564-568页

作者： Hamrang, Zahra Zindy, Egor Clarke, David Pluen, Alain Univ Manchester Sch Pharm & Pharmaceut Sci Manchester Lancs England Univ Manchester Fac Life Sci Wellcome Trust Ctr Cell Matrix Res Manchester Lancs England Univ Manchester Ctr Excellence Biopharmaceut Manchester Lancs England

Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.

关键词： Animals Cattle image processing, computer-assisted/methods Microscopy, Confocal/methods Protein Multimerization Protein Structure, Quaternary Serum Albumin, Bovine/chemistry Time Factors

来源：评论

学校读者我要写书评

暂无评论

ThunderSTORM: a comprehensive imageJ plug-in for PALM and STORM data analysis and super-resolution imaging

引用

BIOINFORMATICS 2014年第16期30卷 2389-2390页

作者： Ovesny, Martin Krizek, Pavel Borkovec, Josef Svindrych, Zdenek K. Hagen, Guy M. Charles Univ Prague Inst Cellular Biol & Pathol Fac Med 1 Prague 12800 Czech Republic

ThunderSTORM is an open-source, interactive and modular plug-in for imageJ designed for automated processing, analysis and visualization of data acquired by single-molecule localization microscopy methods such as photo-activated localization microscopy and stochastic optical reconstruction microscopy. ThunderSTORM offers an extensive collection of processing and post-processing methods so that users can easily adapt the process of analysis to their data. ThunderSTORM also offers a set of tools for creation of simulated data and quantitative performance evaluation of localization algorithms using Monte Carlo simulations.

关键词： Algorithms computer Graphics image processing, computer-assisted/methods Microscopy/methods Microscopy, Fluorescence/methods Monte Carlo Method Software

来源：评论

学校读者我要写书评

暂无评论

On the importance of image formation optics in the design of infrared spectroscopic imaging systems

引用

ANALYST 2014年第16期139卷 4031-4036页

作者： Mayerich, David Van Dijk, Thomas Walsh, Michael J. Schulrnerich, Matthew V. Carney, P. Scott Bhargavad, Rohit Univ Illinois Beckman Inst Adv Sci & Technol Urbana IL 61801 USA Univ Illinois Dept Pathol Chicago IL USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA Univ Illinois Dept Bioengn Urbana IL USA

Infrared spectroscopic imaging provides micron-scale spatial resolution with molecular contrast. While recent work demonstrates that sample morphology affects the recorded spectrum, considerably less attention has been focused on the effects of the optics, including the condenser and objective. This analysis is extremely important, since it will be possible to understand effects on recorded data and provides insight for reducing optical effects through rigorous microscope design. Here, we present a theoretical description and experimental results that demonstrate the effects of commonly-employed cassegranian optics on recorded spectra. We first combine an explicit model of image formation and a method for quantifying and visualizing the deviations in recorded spectra as a function of microscope optics. We then verify these simulations with measurements obtained from spatially heterogeneous samples. The deviation of the computed spectrum from the ideal case is quantified via a map which we call a deviation map. The deviation map is obtained as a function of optical elements by systematic simulations. Examination of deviation maps demonstrates that the optimal optical configuration for minimal deviation is contrary to prevailing practice in which throughput is maximized for an instrument without a sample. This report should be helpful for understanding recorded spectra as a function of the optics, the analytical limits of recorded data determined by the optical design, and potential routes for optimization of imaging systems.

关键词： Algorithms Equipment Design image processing, computer-assisted/instrumentation image processing, computer-assisted/methods Microscopy/instrumentation Microscopy/methods Spectrophotometry, Infrared/instrumentation Spectrophotometry, Infrared/methods

来源：评论

学校读者我要写书评

暂无评论

Categorization training results in shape- and category-selective human neural plasticity

引用

NEURON 2007年第6期53卷 891-903页

作者： Jiang, Xiong Bradley, Evan Rini, Regina A. Zeffiro, Thomas VanMeter, John Riesenhuber, Maximilian Georgetown Univ Med Ctr Dept Neurosci Washington DC 20007 USA Massachusetts Gen Hosp Neural Syst Grp Charlestown MA 02129 USA Georgetown Univ Med Ctr Ctr Funct & Mol Imaging Washington DC 20007 USA

Object category learning is a fundamental ability, requiring the combination of "bottom-up" stimulus-driven with "top-down" task-specific information. It therefore may be a fruitful domain for study of the general neural mechanisms underlying cortical plasticity. A simple model predicts that category learning involves the formation of a task-independent shape-selective representation that provides input to circuits learning the categorization task, with the computationally appealing prediction of facilitated learning of additional, novel tasks over the same stimuli. Using fMRI rapid-adaptation techniques, we find that categorization training (on morphed "cars") induced a significant release from adaptation for small shape changes in lateral occipital cortex irrespective of category membership, compatible with the sharpening of a representation coding for physical appearance. In contrast, an area in lateral prefrontal cortex, selectively activated during categorization, showed sensitivity posttraining to explicit changes in category membership. Further supporting the model, categorization training also improved discrimination performance on the trained stimuli.

关键词： Adaptation, Physiological Adult Brain Mapping Brain Mapping Cerebral Cortex/blood supply Cerebral Cortex/cytology Cerebral Cortex/physiology Discrimination (Psychology)/physiology Female Humans image processing, computer-assisted/methods Learning/physiology Magnetic Resonance Imaging/methods Male Neuronal Plasticity/physiology Oxygen/blood Pattern Recognition, Visual/physiology Photic Stimulation/methods Reaction Time/physiology

来源：评论

学校读者我要写书评

暂无评论

Optimized EPI for fMRI studies of the orbitofrontal cortex

引用

NEUROimage 2003年第2期19卷 430-441页

作者： Deichmann, R Gottfried, JA Hutton, C Turner, R Inst Neurol Wellcome Dept Imaging Neurosci London WC1N 3BG England

A common problem in gradient-echo echo planar imaging (EPI) is the occurrence of image distortions and signal losses caused by susceptibility gradients near air/tissue interfaces. Since EPI is frequently used for functional magnetic resonance imaging experiments based on the blood oxygenation level-dependent effect, functional studies of certain brain regions affected by susceptibility gradients, such as the temporal lobes and the orbitofrontal cortex, may be compromised. In this work a method for signal recovery in certain regions of the orbitofrontal cortex is presented. The influence of in-plane susceptibility gradients is reduced by optimization of the imaging slice orientation. Through-plane susceptibility gradients are partly compensated by means of a moderate preparation gradient pulse similar to z-shimming. In contrast to several other techniques proposed in the literature for reducing susceptibility effects, this method does not compromise the temporal resolution and is therefore applicable to event-related studies. (C) 2003 Elsevier Science (USA). All rights reserved.

关键词： Algorithms Artifacts Brain Mapping/methods Brain Mapping/methods Echo-Planar Imaging/methods Evoked Potentials/physiology Frontal Lobe/physiology Humans image Enhancement/methods image processing, computer-assisted/methods Magnetic Resonance Imaging/methods Mathematical Computing Oxygen Consumption/physiology Reference Values Sensitivity and Specificity

来源：评论

学校读者我要写书评

暂无评论

CellAnimation: an open source MATLAB framework for microscopy assays

引用

BIOINFORMATICS 2012年第1期28卷 138-139页

作者： Georgescu, Walter Wikswo, John P. Quaranta, Vito Vanderbilt Univ Vanderbilt Inst Integrat Biosyst Res & Educ Nashville TN 37240 USA Vanderbilt Univ Dept Biomed Engn Nashville TN 37235 USA Vanderbilt Univ Med Ctr Ctr Canc Syst Biol Vanderbilt Nashville TN USA Vanderbilt Univ Dept Mol Physiol & Biophys Nashville TN 37232 USA Vanderbilt Univ Med Ctr Dept Canc Biol Nashville TN USA Vanderbilt Univ Dept Phys & Astron Nashville TN 37235 USA

Motivation: Advances in microscopy technology have led to the creation of high-throughput microscopes that are capable of generating several hundred gigabytes of images in a few days. Analyzing such wealth of data manually is nearly impossible and requires an automated approach. There are at present a number of open-source and commercial software packages that allow the user to apply algorithms of different degrees of sophistication to the images and extract desired metrics. However, the types of metrics that can be extracted are severely limited by the specific image processing algorithms that the application implements, and by the expertise of the user. In most commercial software, code unavailability prevents implementation by the end user of newly developed algorithms better suited for a particular type of imaging assay. While it is possible to implement new algorithms in opensource software, rewiring an image processing application requires a high degree of expertise. To obviate these limitations, we have developed an open-source high-throughput application that allows implementation of different biological assays such as cell tracking or ancestry recording, through the use of small, relatively simple image processing modules connected into sophisticated imaging pipelines. By connecting modules, non-expert users can apply the particular combination of well-established and novel algorithms developed by us and others that are best suited for each individual assay type. In addition, our data exploration and visualization modules make it easy to discover or select specific cell phenotypes from a heterogeneous population.

关键词： Algorithms Cytological Techniques/methods Cytological Techniques/methods image processing, computer-assisted/methods Internet Microscopy/methods Software

来源：评论

学校读者我要写书评

暂无评论

Naked eye instant reversible sensing of Cu²⁺ and its in situ imaging in live brine shrimp Artemia

引用

ANALYST 2015年第16期140卷 5464-5468页

作者： Nair, Ratish R. Raju, M. Patel, Neha P. Raval, Ishan H. Suresh, E. Haldar, Soumya Chatterjee, Pabitra B. CSIR CSMCRI Analyt Discipline & Centralized Instrumental Faci Bhavnagar 364002 Gujarat India CSIR CSMCRI Marine Biotechnol & Ecol Discipline Bhavnagar 364002 Gujarat India CSIR CSMCRI Acad Sci & Innovat Res Bhavnagar 364002 Gujarat India

A Cu2+-specific colorimetric reversible fluorescent receptor HL was designed and synthesized which showed a naked eye observable colour change from colourless to pink on addition of an aqueous buffer (pH 7.4) solution of 30 ppb Cu2+. Short response time (<= 5 s) and low detection limit (nearly 3 ppb) make HL suitable as a reliable "dip-in" open eye sensor for Cu2+. Bio-imaging application in live brine shrimp Artemia enabled HL to detect Cu2+ at as low as 10 ppb exposure.

关键词： Animals Artemia/metabolism Colorimetry/methods Coloring Agents/chemistry Copper/analysis Copper/chemistry Gastrointestinal Tract/metabolism image processing, computer-assisted/methods Limit of Detection Microscopy, Polarization Water/chemistry

来源：评论

学校读者我要写书评

暂无评论

Microscopy-Based High-Content Screening

引用

CELL 2015年第6期163卷 1314-1325页

作者： Boutros, Michael Heigwer, Florian Laufer, Christina Heidelberg Univ German Canc Res Ctr DKFZ Div Signaling & Funct Genom D-69120 Heidelberg Germany Heidelberg Univ Dept Cell & Mol Biol D-69120 Heidelberg Germany German Canc Consortium DKTK D-69120 Heidelberg Germany

image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, and complex organismal phenotypes. Recently, advances in imaging and image-analysis methodologies have accelerated large-scale perturbation screens. Here, we describe the state of the art for image-based screening experiments and delineate experimental approaches and image-analysis approaches as well as discussing challenges and future directions, including leveraging CRISPR/Cas9-mediated genome engineering.

关键词： Cells/chemistry Cells/cytology CRISPR-Cas Systems High-Throughput Screening Assays image processing, computer-assisted/methods Microscopy Proteins/analysis RNA Interference

来源：评论

学校读者我要写书评

暂无评论

Multiple testing corrections, nonparametric methods, and random field theory

引用

NEUROimage 2012年第2期62卷 811-815页

作者： Nichols, Thomas E. Univ Warwick Warwick Mfg Grp Coventry CV4 7AL W Midlands England Univ Warwick Dept Stat Coventry CV4 7AL W Midlands England

I provide a selective review of the literature on the multiple testing problem in fMRI. By drawing connections with the older modalities, PET in particular, and how software implementations have tracked (or lagged behind) theoretical developments, my narrative aims to give the methodological researcher a historical perspective on this important aspect of fMRI data analysis. (C) 2012 Elsevier Inc. All rights reserved.

关键词： Brain/anatomy & histology Brain/physiology Brain Mapping/history Brain Mapping/history Brain Mapping/methods Brain Mapping/methods History, 20th Century History, 21st Century Humans image processing, computer-assisted/history image processing, computer-assisted/methods Magnetic Resonance Imaging/history Magnetic Resonance Imaging/methods Positron-Emission Tomography/history Positron-Emission Tomography/methods Software/history Statistics, Nonparametric

来源：评论

学校读者我要写书评

暂无评论

Tensor-based morphometry as a neuroimaging biomarker for Alzheimer's disease: An MRI study of 676 AD, MCI, and normal subjects

引用

NEUROimage 2008年第3期43卷 458-469页

作者： Hua, Xue Leow, Alex D. Parikshak, Neelroop Lee, Suh Chiang, Ming-Chang Toga, Arthur W. Jack, Clifford R., Jr. Weiner, Michael W. Thompson, Paul M. Univ Calif Los Angeles Sch Med Dept Neurol Lab Neuro Imaging Los Angeles CA 90095 USA Mayo Clin Coll Med Rochester MN USA UC San Francisco Dept Radiol San Francisco CA USA UC San Francisco Dept Med & Psychiat San Francisco CA USA Dept Vet Affairs Med Ctr Los Angeles CA USA

In one of the largest brain MRI studies to date, we used tensor-based morphometry (TBM) to create 3D maps of structural atrophy in 676 subjects with Alzheimer's disease (AD), mild cognitive impairment (MCI), and healthy elderly controls, scanned as part of the Alzheimer's Disease Neuroimaging Initiative (ADNI). Using inverse-consistent 3D non-linear elastic image registration, we warped 676 individual brain MRI volumes to a population mean geometric template. Jacobian determinant maps were created, revealing the 3D pro. le of local volumetric expansion and compression. We compared the anatomical distribution of atrophy in 165 AD patients (age: 75.6 +/- 7.6 years), 330 MCI subjects (74.8 +/- 7.5), and 181 controls (75.9 +/- 5.1). Brain atrophy in selected regions-of-interest was correlated with clinical measurements - the sum-of-boxes clinical dementia rating (CDR-SB), mini-mental state examination (MMSE), and the logical memory test scores - at voxel level followed by correction for multiple comparisons. Baseline temporal lobe atrophy correlated with current cognitive performance, future cognitive decline, and conversion from MCI to AD over the following year;it predicted future decline even in healthy subjects. Over half of the AD and MCI subjects carried the ApoE4 (apolipoprotein E4) gene, which increases risk for AD;they showed greater hippocampal and temporal lobe deficits than non-carriers. ApoE2 gene carriers - 1/6 of the normal group - showed reduced ventricular expansion, suggesting a protective effect. As an automated image analysis technique, TBM reveals 3D correlations between neuroimaging markers, genes, and future clinical changes, and is highly efficient for large-scale MRI studies. Published by Elsevier Inc.

关键词： Aged Alzheimer Disease/genetics Alzheimer Disease/pathology Apolipoprotein E4/genetics Brain/pathology Brain Mapping/methods Brain Mapping/methods Disease Progression Humans image processing, computer-assisted/methods Magnetic Resonance Imaging Neuropsychological Tests

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：