image analysis provides quantitative data on morphology, cytochemical and histochemical reactions, the location of specific events or reaction sites, and statistical descriptions of the spatial distribution of such ev...
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image analysis provides quantitative data on morphology, cytochemical and histochemical reactions, the location of specific events or reaction sites, and statistical descriptions of the spatial distribution of such events relative to histologic structure. image analytic methodology in correlation with histopathologic knowledge bases is an essential component in the development of an objective basis for histopathologic diagnostic decision making.
We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial ...
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We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial Sau3AI digest of flow-sorted chromosomes from a mouse-human hybrid cell line. Sixty-three of these (89%) gave a signal only on chromosome 17. The 40 cosmids producing the most distinct hybridization signals in metaphase and interphase cells were precisely mapped using digital image analysis. An additional 20 cosmids, previously mapped by linkage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linkage analysis. (C) 1994 Academic Press, Inc.
A letter to the editor in response to the article 'Dynamic populations of dendritic cell-specific ICAM-3 grabbing non-integrin-positive immature dendritic cells and liver/lymph node-specific ICAM-3 grabbing nonint...
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A letter to the editor in response to the article 'Dynamic populations of dendritic cell-specific ICAM-3 grabbing non-integrin-positive immature dendritic cells and liver/lymph node-specific ICAM-3 grabbing nonintegrin-positive endothelial cells in the outer zones of the paracortex of human lymph nodes' in the 2004 issue is presented.
Dental radiographs are amenable to digital image processing and analysis for both subjective interpretation and quantification of scene features. Four general-purpose image processing programs for the Apple Macintosh ...
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Prediction of bacterial deposition rates onto substrates in natural aquatic systems is quite challenging because of the inherent complexity of such systems. In this study we compare experimental deposition kinetics of...
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Prediction of bacterial deposition rates onto substrates in natural aquatic systems is quite challenging because of the inherent complexity of such systems. In this study we compare experimental deposition kinetics of nonmotile bacteria (Pseudomonas aeruginosa) on an alginate-coated substrate in a radial stagnation point flow (RSPF) system to predictions based on DLVO theory. The "softness" of the surface layer of the bacteria and alginate-coated substrate was considered in the calculations of their electrokinetic surface properties, and the relevance of both the classical zeta potential and the outer surface potential as surrogates for surface potential was investigated. Independent of the used electrical potentials, we showed that significant discrepancies exist between theory and experiments. Analysis of microscopic images in the RSPF system has demonstrated, for the first time, that irreversible deposition of particles or cells entrapped in the secondary energy minimum can occur on the alginate layer, despite the hydrodynamic forces resulting from the radial flow in the RSPF system. It is suggested that polymeric structures associated with the surface of the particle/cell and the alginate-coated substrate are responsible for the transition between the secondary minimum and primary energy well. This mode of deposition is likely to be important in the deposition of microorganisms in complex aquatic systems.
Electron microscopy tomography requires high-tilt projections. However, the variation in defocus over a highly tilted specimen results in a blurred micrograph, where the blur is spatially varying perpendicular to the ...
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Electron microscopy tomography requires high-tilt projections. However, the variation in defocus over a highly tilted specimen results in a blurred micrograph, where the blur is spatially varying perpendicular to the tilt axis. This spatially varying blur can cause the micrograph to deviate from an acceptable approximation of the projection of the specimen's density function. In practice, this has been one factor limiting reconstructions of large cellular areas. We derive an algorithm to restore, or deblur, high-tilt electron micrographs using multiple defocused versions of the micrograph. This algorithm consists of digitization and registration of the micrographs, identification of the defocus blur using either a theoretical model or a zero-tilt focus series, and spatially varying restoration using the multiple high-tilt defocused micrographs. Numerical results using simulated and real data are presented, demonstrating the effectiveness of this algorithm.
Extracting characteristic dimensions from mounded surfaces such as grain size or intergrain lengths is usually made by statistical analysis. Different statistical functions are used in the literature to extract charac...
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Extracting characteristic dimensions from mounded surfaces such as grain size or intergrain lengths is usually made by statistical analysis. Different statistical functions are used in the literature to extract characteristic lengths. The main issue is that depending on the choice of the statistical function the results can be very different. In this paper, we demonstrate using a series of model mounded surfaces for which characteristic dimensions are known, that a method (namely, interfacial differential function, IDF) is the most effective method to determine the different characteristic lengths. The influence on the statistical treatment of the variation of the different characteristic lengths is then studied and confirms the ability of the IDF analysis. The IDF method was used to analyze the evolution of ultrathin gold film morphology as function of deposition temperature. This approach allows us to demonstrate that the roughness increase with deposition temperature is mainly due to a grain height increase and not to a grain coarsening phenomena as it was claimed before.
O-6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O-6-benzylguanine (O-6-BG) derivatives for multicolor live cell imaging approaches. Here, we char...
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O-6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O-6-benzylguanine (O-6-BG) derivatives for multicolor live cell imaging approaches. Here, we characterize several new BG fluorophores suitable for in vivo AGT labeling that display fluorescence emission maxima covering the visible spectrum from 472 to 673 nm, thereby extending the spectral limits set by fluorescent proteins. We show that the photostability of the cell-permeable dyes BG Rhodamine Green (BG505) and CP tetramethylrhodamine (CP-TMR) is in the range of enhanced green fluorescent protein (EGFP) and monomeric red fluorescent protein (mRFP), and that BG diethylaminomethyl coumarin (BGDEAC) a derivative of coumarin, is even more stable than enhanced cyan fluorescent protein (ECFP). Due to the increasing number of new BG derivatives with interesting fluorescence properties, such as far-red emission, fluorescence labeling of AGT fusion proteins is becoming a versatile alternative to existing live cell imaging approaches.
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