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Two-photon images reveal unique texture features for label-free identification of ovarian cancer peritoneal metastases

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BIOMEDICAL OPTICS EXPRESS 2019年第9期10卷 4479-4488页

作者： Pouli, Dimitra Genega, Elizabeth M. Sullivan, Travis B. Rieger-Christ, Kimberly M. Wright, Valena Georgakoudi, Irene Schnelldorfer, Thomas Tufts Univ Dept Biomed Engn Medford MA 02155 USA Tufts Med Ctr Dept Pathol & Lab Med Boston MA 02111 USA Lahey Hosp & Med Ctr Dept Translat Res Burlington MA 01805 USA Lahey Hosp & Med Ctr Dept Surg Burlington MA 01805 USA Beth Israel Deaconess Med Ctr Dept Pathol Boston MA 02215 USA Harvard Univ Harvard Med Sch Boston MA 02115 USA

For cancer patients, treatment selection fundamentally relies on staging, with "understaging" considered a common problem. Imaging modalities that can complement conventional white-light laparoscopy are needed to detect more accurately small metastatic lesions in patients undergoing operative cancer care. Biopsies from healthy parietal peritoneum and ovarian peritoneal metastases obtained from 8 patients were imaged employing a two-photon laser scanning microscope to generate collagen-second harmonic generation (SHG) and fluorescence images at 755 nm and 900 nm excitation and 460 +/- 20 nm and 525 +/- 25 nm emission. Forty-one images were analyzed by automated image processing algorithms and statistical textural analysis techniques, namely gray level co-occurrence matrices. Two textural features (contrast and correlation) were employed to describe the spatial intensity variations within the captured images and outcomes were used for discriminant analysis. We found that healthy tissues displayed large variations in contrast and correlation features as a function of distance, corresponding to repetitive, increased local intensity fluctuations. Metastatic tissue images exhibited decreased contrast and correlation related values, representing more uniform intensity patterns and smaller fibers, indicating the destruction of the healthy stroma by the cancerous infiltration. The textural outcomes resulted in high classification accuracy as evaluated quantitatively by discriminant analysis. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： Harmonic generation image processing algorithms Imaging systems Laser scanning Scanning microscopy White light

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Computer-vision based intelligent adaptive transmission for optical wireless communication

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OPTICS EXPRESS 2019年第6期27卷 7979-7987页

作者： Huang, Zhitong Zu, Lijia Zhou, Zihao Tang, Xiao Ji, Yuefeng Beijing Univ Posts & Telecommun SICE State Key Lab Informat Photon & Opt Commun IPOC Beijing 100876 Peoples R China

Optical wireless communication (OWC) has been presented as a promising candidate for future space-air-ground-ocean-integrated communication. However, the OWC is quite sensitive to the variation of the channel transmission characteristics. The light beam absorption and the scattering in the transmission media affect not only the channel feature, but also the imaging quality. Thus, there is an inherent relationship between the OWC performance and the optical imaging quality. Based on this consideration we firstly present the idea of introducing computer vision mechanisms into the OWC systems, and then propose a computer vision-based multi-domain cooperative adjustment (CV-MDCA) mechanism's functional modules to realize the intelligent adaptive transmission in OWC systems. The CV-MDCA mechanism arc specifically designed, with the emphasis on how to quantitatively determine the exact on-line channel quality from the captured images by using effective computer vision schemes. Two groups of experiments, the indoor-simulated underwater visible light communication and the outdoor-practical atmospheric free-space optics, arc implemented in order to evaluate the presented CV-MDCA mechanism's performance. The results not only validate the feasibility to determine the channel quality, according to the captured channel images, but also reveal the presented three computer vision-based criteria's limitations. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： Edge detection image processing image processing algorithms image quality Nonlinear impairments Optical wireless communication

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Three-dimensional single-photon imaging through obscurants

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OPTICS EXPRESS 2019年第4期27卷 4590-4611页

作者： Tobin, Rachael Halimi, Abderrahim McCarthy, Aongus Laurenzis, Martin Christnacher, Frank Buller, Gerald S. Heriot Watt Univ Sch Engn & Phys Sci Edinburgh EH14 4AS Midlothian Scotland French German Res Inst St Louis 5 Rue Gen Cassagnou F-68301 St Louis France

We investigate the depth imaging of objects through various densities of different obscurants (water fog. glycol-based vapor, and incendiary smoke) using a time-correlated single-photon detection system which had an operating wavelength of 1550 nm and an average optical output power of approximately 1.5 mW. It consisted of a monostatic scanning transceiver unit used in conjunction with a picosecond laser source and an individual Peltier-cooled InGaAs/InP single-photon avalanche diode (SPAD) detector. We acquired depth and intensity data of targets imaged through distances of up to 24 meters for the different obscurants. We compare several statistical algorithms which reconstruct both the depth and intensity images for short data acquisition times, including very low signal returns in the photon-starved regime. (C) Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.

关键词： Atmospheric scattering Attenuation coefficient image processing algorithms image quality Laser beam propagation Optical components

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Efficient polarization direction measurement by utilizing the polarization axis finder and digital image processing

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OPTICS LETTERS 2018年第12期43卷 2969-2972页

作者： Lei, Bing Liu, Shugang Natl Univ Def Technol Coll Adv Interdisciplinary Studies Changsha 410073 Hunan Peoples R China Heilongjiang Univ Coll Elect Engn Harbin 150080 Heilongjiang Peoples R China

We report a new quantitative measurement method of polarization direction based on the polarization axis finder (PAF) and digital image processing. The PAF acts as an azimuthal analyzer to determine the polarization direction of linearly polarized light and form an "hourglass" intensity pattern, and a digital camera is employed to record the pattern, and then the lightwave's polarization direction is obtained accurately by analyzing the pattern with a specially designed digital image processing algorithm. The quantitative measuring experiments have been carried out by validating the standard quartz plates with known rotation angles, and the measurement accuracy has reached 0.01 degrees. (C) 2018 Optical Society of America

关键词： Digital filtering Digital image processing image processing image processing algorithms Light fields Polarized light

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Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

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OPTICS EXPRESS 2019年第3期27卷 2235-2247页

作者： Macias-Romero, Carlos Teulon, Claire Didier, Marie Roke, Sylvie Ecole Polytech Fed Lausanne Lab Fundamental BioPhoton LBP Inst Bioengn IBI CH-1015 Lausanne Switzerland Ecole Polytech Fed Lausanne Inst Mat Sci IMX Sch Engn STI CH-1015 Lausanne Switzerland Ecole Polytech Fed Lausanne Lausanne Ctr Ultrafast Sci LACUS CH-1015 Lausanne Switzerland

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroimaging with specific sensitivity to microtubules. We study here the structural organization of microtubules in living neurons using a wide-field multiphoton microscope that performs 3D imaging using a structured illumination. This microscope allows label-free high throughput imaging of living mammalian neurons. We show that we can image structural correlations by taking advantage of the structured illumination and the coherence of the emitted light. The result allows us to study the microtubule organization throughout the development of the neuron and to differentiate between the regions of the cytoskeleton in the matured neuron. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： image processing algorithms Live cell imaging Phase contrast Spatial frequency Three dimensional image processing Three dimensional imaging

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Translational and rotational manipulation of filamentous cells using optically driven microrobots

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OPTICS EXPRESS 2019年第12期27卷 16475-16482页

作者： Hu, Songyu Hu, Ruifeng Dong, Xiaobin Wei, Tanyong Chen, Shuxun Sun, Dong Zhejiang Univ Coll Mech Engn State Key Lab Fluid Power & Mechatron Syst Hangzhou Zhejiang Peoples R China Zhejiang Univ Coll Mech Engn Key Lab 3D Printing Proc & Equipment Zhejiang Pro Hangzhou Zhejiang Peoples R China Univ Pittsburgh Dept Mech Engn & Mat Sci Pittsburgh PA 15260 USA Shenzhen Univ Coll Mechatron & Control Engn Shenzhen Peoples R China City Univ Hong Kong Dept Biomed Engn Hong Kong Peoples R China

Optical cell manipulation has become increasingly valuable in cell-based assays. In this paper, we demonstrate the translational and rotational manipulation of filamentous cells using multiple cooperative microrobots automatically driven by holographic optical tweezers. The photodamage of the cells due to direct irradiation of the laser beam can be effectively avoided. The proposed method will enable fruitful biomedical applications where precise cell manipulation and less photodamage are required. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： Femtosecond fiber lasers High numerical aperture optics image processing algorithms Laser beams Optical trapping Optical tweezers

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Correlation-based receiver for optical camera communications

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OPTICS EXPRESS 2019年第14期27卷 19150-19155页

作者： Jurado-Verdu, Cristo Matus, Vicente Rabadan, Jose Guerra, Victor Perez-Jimenez, Rafael Univ Las Palmas Gran Canaria IDeTIC Las Palmas Gran Canaria Spain

In color-multiplexed optical camera communications (OCC) systems, data acquisition is restricted by the image processing algorithm capability for fast source recognition, region-of-interest (ROI) detection and tracking, packet synchronization within ROI, estimation of inter-channel interference and threshold computation. In this work, a novel modulation scheme for a practical RGB-LED-based OCC system is presented. The four above-described tasks are held simultaneously. Using confined spatial correlation of well-defined reference signals within the frame's color channels is possible to obtain a fully operating link with low computational complexity algorithms. Prior channel adaptation also grants a substantial increase in the attainable data rate, making the system more robust to interferences. (c) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： image processing image processing algorithms LED sources Optical receivers Space division multiplexing Visible light communications

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Femtosecond laser enabled selective micro-holes drilling on the multicore-fiber facet for displacement sensor application

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OPTICS EXPRESS 2019年第8期27卷 10777-10786页

作者： Zhang, Cong Jiang, Zhisheng Fu, Songnian Tang, Ming Tong, Weijun Liu, Deming Huazhong Univ Sci & Technol Wuhan Natl Lab Optoelect Wuhan 430074 Hubei Peoples R China Huazhong Univ Sci & Technol Sch Opt & Elect Informat Wuhan 430074 Hubei Peoples R China Yangtze Opt Fiber & Cable Joint Stock Ltd Co YOFC R&D Ctr State Key Lab Opt Fiber & Cable Manufacture Techn Wuhan 430074 Hubei Peoples R China

We experimentally demonstrate a femtosecond laser enabled selective micro-holes drilling technique on the multicore-fiber facet. The precise position of individual cores at the seven-core fiber facet is initially locked by the image processing algorithm, and then six micro-holes are successfully fabricated after the pulse energy of femtosecond laser is optimized. Meanwhile, the use of fabricated seven-core fiber for the application of reflective intensity-modulated fiber optics displacement sensor (RIM-FODS) is comprehensively investigated. By using the beam propagation method (BPM), we theoretically investigate the effect of micro-hole depth on the RIM-FODS performance, in terms of both dead zone and measurement range. We identify that, with the increase of micro-hole depth, the dead zone range can be substantially reduced at the expense of measurement range reduction. However, multiple micro-holes with a successive depth difference can overcome such problem. When the micro-holes with depths of 5, 10, 15, 20, 25, 30 mu m are fabricated on the seven-core fiber facet, and the dead zone range can be substantially reduced from 150 mu m to 20 mu m, together with an extension of measurement range from 250 mu m to 400 mu m. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： Femtosecond fiber lasers Femtosecond lasers image processing algorithms Single mode fibers Surface enhanced Raman scattering Whispering gallery modes

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Miniaturized omnidirectional flexible side-view endoscope for rapid monitoring of thin tubular biostructures

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BIOMEDICAL OPTICS EXPRESS 2019年第5期10卷 2279-2289页

作者： Paulson, Bjorn Lee, SangHwa Kim, Youngkyu Moon, Youngjin Kim, Jun Ki Asan Med Ctr Biomed Engn Res Ctr Asan Inst Life Sci 88 Olymp Ro 43 Gil Seoul 05505 South Korea Univ Ulsan Coll Med Dept Convergence Med 88 Olymp Ro 43 Gil Seoul 05505 South Korea

Endoscopic imaging allows longitudinal observation of epithelial pathologies in tubular organs throughout the body. However, the imaging and optical diagnosis of tubular biostructures such as small animal models and small pediatric organs require appropriately miniaturized devices. A miniaturized catadioptric flexible side-view endoscope is proposed with omnidirectional field of view (FOV) in the transverse direction and sub-nun-scale feature resolution. The FOV in the longitudinal direction is 50 degrees. images are unwrapped and stitched together to form composite images of the target by two different algorithms, revealing a composite FOV of more than 3.5 cm x 360 degrees. The endoscope is well suited for minimally invasive rapid monitoring of thin tubular organs in pediatric patients, as well as for imaging of small animal disease models at near-cellular resolution. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

关键词： Brain imaging Gastrointestinal imaging image metrics image processing algorithms image quality Modulation transfer function

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Correction for color artifacts using the RGB intersection and the weighted bilinear interpolation

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APPLIED OPTICS 2019年第29期58卷 8083-8091页

作者： Wang, Xiuhua Jia, Xinyue Zhou, Wei Qin, Xiaoyun Guo, Hanming Univ Shanghai ForSci & Technol Engn Res Ctr Opt Instrument & Syst Shanghai Key Lab Modern Opt Syst Minist EducSch Opt Elect & Comp Engn 516 Jungong Rd Shanghai 200093 Peoples R China

Due to the defects of optical systems, image sensors, and imperfect algorithms for image acquisition, compression, and restoration, color artifacts often appear in images obtained by imaging devices such as digital cameras and scanners. Moreover, color artifacts are difficult to eliminate because of technical limitations, even in some mature commercial cameras. On the basis of red, green, and blue (RGB) intersection (RGBI), a correction method for color artifacts is proposed in this paper, where the RGB intersection-based method can effectively detect various types of color artifacts. Also, by combining the object information with weighted bilinear interpolation, the continuity of the image is kept while restoring the real color. Experiments demonstrate that the RGBI method, which is applicable to all color images, can eliminate various types of color artifacts with accurate detection and less artifact residue, even if the image has severe color distortion or the areas of the color artifacts are small and discrete. (C) 2019 Optical Society of America

关键词： Color difference Color spaces Digital imaging image processing algorithms image quality image sensors

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