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Qualitative and quantitative interpretation of SEM image using digital image processing

JOURNAL OF MICROSCOPY 2016年第1期264卷 102-124页

作者： Saladra, Dawid Kopernik, Magdalena AGH Univ Sci & Technol Al Mickiewicza 30 PL-30059 Krakow Poland

The aim of the this study is improvement of qualitative and quantitative analysis of scanning electron microscope micrographs by development of computer program, which enables automatic crack analysis of scanning electron microscopy (SEM) micrographs. Micromechanical tests of pneumatic ventricular assist devices result in a large number of micrographs. Therefore, the analysis must be automatic. Tests for athrombogenic titanium nitride/gold coatings deposited on polymeric substrates (Bionate II) are performed. These tests include microshear, microtension and fatigue analysis. Anisotropic surface defects observed in the SEM micrographs require support for qualitative and quantitative interpretation. Improvement of qualitative analysis of scanning electron microscope images was achieved by a set of computational tools that includes binarization, simplified expanding, expanding, simple image statistic thresholding, the filters Laplacian 1, and Laplacian 2, Otsu and reverse binarization. Several modifications of the known image processing techniques and combinations of the selected image processing techniques were applied. The introduced quantitative analysis of digital scanning electron microscope images enables computation of stereological parameters such as area, crack angle, crack length, and total crack length per unit area. This study also compares the functionality of the developed computer program of digital image processing with existing applications. The described pre- and postprocessing may be helpful in scanning electron microscopy and transmission electron microscopy surface investigations.

关键词： Coatings digital image image processing algorithms micromechanical test scanning electron microscopy

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Mobile Microscope for Quantitative Fluorescence Sensing Through Highly Autofluorescent and Scattering Media

Mobile Microscope for Quantitative Fluorescence Sensing Thro...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Gorocs, Zoltan Rivenson, Yair Koydemir, Hatice Ceylan Tseng, Derek Troy, Tamara L. Demas, Vasiliki Ozcan, Aydogan Univ Calif Los Angeles Dept Elect Engn Los Angeles CA 90095 USA Univ Calif Los Angeles Dept Bioengn Los Angeles CA 90095 USA Univ Calif Los Angeles Calif NanoSyst Inst CNSI Los Angeles CA 90095 USA Verily Life Sci LLC Mountain View CA 94043 USA

We created a mobile fluorescence microscope weighing <40 grams and demonstrated a computational sensing method for quantitative measurement of fluorescent dyes through highly autofluorescent, scattering and absorbi... 详细信息

ISBN: (纸本)9781943580279

关键词： Computation methods Diode lasers Fluorescence microscopy image processing image processing algorithms Scattering media

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End-Fire Silicon Optical Phased Arrays for Infrared Neural Stimulation Applications

End-Fire Silicon Optical Phased Arrays for Infrared Neural S...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Kossey, Michael R. Alt, Shannon Rizk, Charbel Foster, Amy C. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

We propose infrared neural stimulation as an application for end-fire integrated optical beam steering devices. We show some initial results and discuss its implications for the future development of the technique.

ISBN: (纸本)9781943580279

关键词： Array waveguide devices Beam steering image processing algorithms Laser light Light beams Phased arrays

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Implementation of Parallel Morphological Filter with Different Structuring Elements

Implementation of Parallel Morphological Filter with Differe...

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IEEE International Conference on Power, Control, Signals and Instrumentation Engineering (IEEE ICPCSI)

作者： Pandey, Ritesh Kumar Mathurkar, S. S. GCOE Elect & Telecommun Dept Amravati India

ISBN: (纸本)9781538608142

This paper gives comparative analysis of parallel morphological filter with previously designed morphological filters. In this paper, implementation is done for gray image using various structuring elements. In past, multiple systems have been developed that have huge latency and fewer throughputs. This system reduced latency and improved through by using parallel approach. It provides a programmable and efficient implementation of basic morphological operators such as Dilation and Erosion using efficient parallel technique for different applications. It reduces processing time with respect to image dimension. Here performance analysis is based on different shape and size of structuring elements which support parallelism. MATLAB R2013a and Xilinx Design Suite 13.1 ISE are used for synthesizing this architecture that is verified by using Xilinx ISIM Simulator and prototyped on Spartan 3E FPGA Board. The proposed architecture is examined on different gray scale images.

关键词： Morphological filters image processing algorithms MATLAB simulation FPGA model Parallel Morphological Filter

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Automated Detection and Enumeration of Waterborne Pathogens Using Mobile Phone Microscopy and Machine Learning

Automated Detection and Enumeration of Waterborne Pathogens ...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Koydemir, Hatice Ceylan Feng, Steve Liang, Kyle Nadkarni, Rohan Benien, Parul Ozcan, Aydogan Univ Calif Los Angeles Elect Engn Dept Los Angeles CA 90095 USA Univ Calif Los Angeles Bioengn Dept Los Angeles CA 90095 USA Univ Calif Los Angeles Calif NanoSystems Inst CNSI Los Angeles CA 90095 USA Univ Calif Los Angeles Comp Sci & Engn Dept Los Angeles CA 90095 USA

We present a field portable and cost-effective smartphone based microscopy platform for rapid and sensitive detection and automated counting of waterborne pathogens, i.e., Giardia lamblia cysts, in large volume water ... 详细信息

ISBN: (纸本)9781943580279

关键词： Fluorescence microscopy image processing image processing algorithms Microscopy Optical devices Optical microscopy

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A Microwave Photonics-based Inverse Synthetic Aperture Radar System

A Microwave Photonics-based Inverse Synthetic Aperture Radar...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Xiao, Xuedi Li, Shangyuan Chen, Boyu Yang, Xiao Wu, Dexin Xue, Xiaoxiao Zheng, Xiaoping Zhou, Bingkun Tsinghua Univ Tsinghua Natl Lab Informat Sci & Technol Dept Elect Engn Beijing 10084 Peoples R China

We demonstrate a microwave imaging radar experimental platform, using photonic generation of linear frequency modulated waveform centered at 10GHz with 4GHz bandwidth. Distance and velocity resolution of about 5cm and... 详细信息

ISBN: (纸本)9781943580279

关键词： image processing image processing algorithms Microwaves Radar imaging Signal processing Synthetic aperture radar

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Increased precision of zernike fit to noisy truncated phase maps

Increased precision of zernike fit to noisy truncated phase ...

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Optical Design and Fabrication, Freeform 2017

作者： Adelsberger, Kathleen Mack, Stephen Tienvieri, Ted Rich, Tim Corning Tropel Corporation 60 O'Connor Rd FairportNY14450 United States

When a polynomial set fails to meet the conditions of orthogonality, errors appear in the calculated coefficients. Restoring orthogonality by fitting a custom Zernike set to noisy phase maps provides increased precisi... 详细信息

ISBN: (纸本)9781943580316

关键词： image processing algorithms Lens design Pupil Tolerancing Zernike polynomials

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Investigation on vibration excitation of debonded sandwich structures using time-average digital holography

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APPLIED OPTICS 2017年第13期56卷 F7-F13页

作者： Thomas, Binu P. Pillai, S. Annamala Narayanamurthy, C. S. Indian Inst Space Sci & Technol Dept Aerosp Engn Thiruvananthapuram 695547 Kerala India Vikram Sarabhai Space Ctr Expt Mech Div Thiruvananthapuram 695022 Kerala India Rajadhani Inst Engn & Technol Dept Mech Engn Thiruvananthapuram 695102 Kerala India Indian Inst Space Sci & Technol Dept Phys Thiruvananthapuram 695547 Kerala India

Sandwich structures, in the modern aerospace industry, are more sought after due to their high strength to stiffness ratio resulting in significant weight gains. Optical techniques like time-average holography and shearography are preferred in industries for inspection of huge sandwich and composite panels because of whole-field (full coverage) inspection in a lesser time leading to large savings in cost. These techniques conventionally use sinusoidal frequency sweep to capture the local resonance of defective regions. This paper highlights the difficulties with the conventional approach of time-average digital holography (TADH) and proposes a novel defect identification strategy through square wave excitation. The proposed method enhances the speed and accuracy of inspection;thereby it saves cost and increases confidence level. Extensive experiments have been carried out using honeycomb sandwich panels to demonstrate the methodology. (C) 2017 Optical Society of America

关键词： Digital holographic imaging Holographic interferometry image processing image processing algorithms Industrial inspection Off axis digital holography

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Centroid stabilization for laser alignment to corner cubes: designing a matched filter

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APPLIED OPTICS 2017年第1期56卷 A41-A51页

作者： Awwal, Abdul A. S. Bliss, Erlan Brunton, Gordon Kamm, Victoria Miller Leach, Richard R., Jr. Lowe-Webb, Roger Roberts, Randy Wilhelmsen, Karl Lawrence Livermore Natl Lab Livermore CA 94551 USA

Automation of image-based alignment of National Ignition Facility high energy laser beams is providing the capability of executing multiple target shots per day. One important alignment is beam centration through the second and third harmonic generating crystals in the final optics assembly (FOA), which employs two retroreflecting corner cubes as centering references for each beam. Beam-to-beam variations and systematic beam changes over time in the FOA corner cube images can lead to a reduction in accuracy as well as increased convergence durations for the template-based position detector. A systematic approach is described that maintains FOA corner cube templates and guarantees stable position estimation. (C) 2016 Optical Society of America

关键词： High power lasers image processing image processing algorithms Laser amplifiers Laser beams Solid state lasers

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Stent optical inspection system calibration and performance

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APPLIED OPTICS 2017年第9期56卷 D134-D141页

作者： Bermudez, Carlos Laguarta, Ferran Cadevall, Cristina Matilla, Aitor Ibanez, Sergi Artigas, Roger Tech Univ Catalonia UPC Ctr Sensors Instruments & Syst Dev CD6 Rambla St Nebridi 10 E-08222 Terrassa Spain Sensofar Tech SL Parc Audiovisual CatalunyaCtra BV1274 Km 1 E-08225 Terrassa Spain Sensofar Med SL Parc Audiovisual CatalunyaCtra BV1274 Km 1 E-08225 Terrassa Spain

Implantable medical devices, such as stents, have to be inspected 100% so no defective ones are implanted into a human body. In this paper, a novel optical stent inspection system is presented. By the combination of a high numerical aperture microscope, a triple illumination system, a rotational stage, and a CMOS camera, unrolled sections of the outer and inner surfaces of the stent are obtained with high resolution at high speed with a linescan approach. In this paper, a comparison between the conventional microscope image formation and this new approach is shown. A calibration process and the investigation of the error sources that lead to inaccuracies of the critical dimension measurements are presented. (C) 2017 Optical Society of America.

关键词： CMOS cameras High numerical aperture optics image processing algorithms image quality Line scan cameras Optical microscopy

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