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作者： Hsiang-Ting Hsu Alexandre F. Carisey Jordan S. Orange

Natural killer (NK) cells contain specialized lysosome-related organelles termed lytic granules allowing them to mediate cytotoxicity against tumorigenic or virally infected target cells. NK cells polarize their lytic granules toward a target cell via the microtubule-organizing center (MTOC). Prior to that, however, lytic granules converge to the MTOC along microtubules utilizing minus-end-directed microtubule motors. Herein we describe how to visualize and quantify lytic granule convergence using confocal microscopy to gain quantitative insight into NK cell cytotoxicity and its regulation. less

关键词： Confocal Microscopy Cell Separation And Isolation 3D Imaging Live Cell Imaging Biosensor technique Time Lapse Microscopy Fiji Image Analysis Cell And Tissue Culture Homo sapiens NK-92 Immunological synapse Convergence MTOC Lytic granules mathematical algorithm Anti-α-tubulin Anti-rabbit IgG (H+L) Anti-human Perforin

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An Approach to Wheel Loader Bucket Raise Time Detection

An Approach to Wheel Loader Bucket Raise Time Detection

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2016 3rd International Conference on Engineering Technology and Application（ICETA 2016）

作者： Qi Liao Wubin Xu Bing Li Qingbao Hua School of Mechanical Engineering Guangxi University of Science and Technology Liugong Machinery Co. Ltd.

An approach to wheel loader bucket raise time recognition based on mathematical algorithms is presented in this *** loader bucket raise time is one of the most essential parameters in total cycling time,which provides the work efficiency for wheel *** loader bucket raise time is detected by the application of a simple mathematical algorithm after wavelet ***,a signal of wheel loader total cycling time signal(original signal) which includes bucket raise time,bucket dump time and bucket lower time was selected,and then it was processed by the wavelet ***,we could get wavelet decomposition approximation signal a5,and mathematical algorithm was implemented at simple computational cost by signal form,which detected the singular points from signal ***,original signal based on threshold decision combined singular points to detect the onset point and offset point of bucket raise segment in original *** then we could get bucket raise *** records were from a loader factory online detection *** result demonstrated that the detection rate is more than 95%.This study implemented automatic detection of wheel loader bucket raise time.

关键词： wheel loader bucket raise time wavelet transform mathematical algorithm

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On Computing the Measurable Amounts of the Two Jugs Problem

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MATHEMATICS IN COMPUTER SCIENCE 2015年第4期9卷 453-459页

作者： Man, Yiu-Kwong HKIEd Dept Math & Informat Technol 10 Lo Ping Rd Tai Po Hong Kong Peoples R China

The two jugs problem is a classic problem in mathematics and computer sciences. In this paper, we introduce a new algorithm to solve the general two jugs problem. This algorithm has the advantage that one can easily apply it to determine all measurable amounts by the given jugs and provides us insight on the hidden relationship between the two different approaches to tackle the problem, namely the attempt to fill the smaller jug or the larger jug first. We also discuss how to implement this algorithm in Excel and visualize the generated outputs by means of staircase diagrams. Some basic properties of the measurable amounts by the jugs will also be described, together with some examples.

关键词： mathematical algorithm Jugs filling problem Measurable amounts Diophantine equation Staircase diagram

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Automating motion trajectory Of crane-lifted loads

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AUTOMATION IN CONSTRUCTION 2014年 45卷 178-186页

作者： Olearczyk, Jacek Bouferguene, Ahmed Al-Hussein, Mohamed Hermann, Ulrich (Rick) Univ Alberta Dept Civil & Environm Engn Edmonton AB T6G 2W2 Canada Univ Alberta Edmonton AB Canada PCL Ind Management Inc Edmonton AB T6E 3P4 Canada

Crane Lifting Path Planning (CLPP) is an important task, especially in congested construction sites. This activity becomes extremely complex since, as the project advances, new (and sometimes unpredicted) constraints may occur. These constraints force lift engineers to explore the possibility of alternative paths for the objects still lobe moved. Under severe time constraints, manual analysis of such paths is practically impossible, which makes it necessary to rely on computer implementations in order to avoid project delays. From a mathematical standpoint, if the crane payload trajectory is to be defined analytically, the polar coordinate system,(r, theta), is naturally the most suitable. As a result, this paper proposes an algorithm for CLPP in which the path is represented as a piece-wise continuous function where each portion is defined either by constant radius (rotation of the load) or a constant angle (translation of the load). In other words, rather than forcing a crane to adapt to unnatural rectilinear trajectories as obtained by traditional path searching procedures, it is the trajectory that is adapted to the crane motion. In fact, even though (mathematically) a precise balance between the rates of rotation and translation of the jib (or boom) will make the payload follow any continuous path (regardless of its complexity), the coupling between the rotations and translations increases the difficulty of the lifting activity. However, a crane lifting path in which these motions are uncoupled will lead to less stringent requirements in terms of controlling the balance between rotations and translations. (C) 2014 Elsevier B.V. All rights reserved.

关键词： Mobile crane lifts Object trajectory Polar coordinates mathematical algorithm

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Evolution of the crane selection and on-site utilization process for modular construction multilifts

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AUTOMATION IN CONSTRUCTION 2014年 43卷 59-72页

作者： Olearczyk, Jacek Al-Hussein, Mohamed Bouferguene, Ahmed Univ Alberta Dept Civil & Environm Engn Hole Sch Construct Markin CNRL Nat Resources Engn Facil 4 110H Edmonton AB T6G 2W2 Canada Univ Alberta Edmonton AB T6G 2W2 Canada

Modular construction technology has been used in building construction for decades, having been widely utilized on sites with high traffic volume, restricted accessibility/storage, or business operations which cannot be interrupted by long construction time. A key challenge in this method of construction lies in planning and executing lifts within a short timeframe. In this regard, proper crane selection and site layout optimization can significantly increase productivity and shorten the lifting schedule. This paper thus proposes a methodology for the crane selection process and introduces mathematical algorithms to assess the construction of multi-lift operations. The modular lift process is divided into three stages: crane load and capacity check, crane location, and boom and superlift clearance. Each stage's parameters are introduced, analyzed, and graphically explained. The methodology logic is supported by a generalized mathematical algorithm and is applied and tested on a case study involving the construction of five three-storey dormitories in 10 working days for Muhlenberg College in Allentown, Pennsylvania. (C) 2012 Elsevier B.V. All rights reserved.

关键词： Mobile crane lift Lift logistics Site layout mathematical algorithm Modular construction

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Who are the members of the international club?

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APPLIED ECONOMICS 2014年第14期46卷 1582-1585页

作者： Miyakoshi, Tatsuyoshi Suzuki, Kenichi Hosei Univ Fac Sci & Engn Tokyo 1848584 Japan Tohoku Univ Dept Econ Sendai Miyagi 980 Japan

This article proposes pragmatic methods that incorporate recent contributions to public good theory to identify the members of the international club and how they select new members. This article also suggests simple ... 详细信息

关键词： club members forming a club mathematical algorithm aggregate replacement function

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mathematical algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay

Mathematical Algorithms for Automatic Search, Recognition, a...

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作者： Sergey S. Zadorozhny Nikolai N. Martynov

Accuracy of spot detection and quantification plays a critical role in the analysis of ELISPOT data. Differences in staining intensities of spots and their morphological variations make it difficult developing a reliable software application. We have developed an image recognition method allowing for the automatic detection of round objects (spots) on ELISPOT images independently of the registration conditions. The emphasis is done on objects of elliptical shape which is typical for a wide range of spots that can be analyzed by both monochrome and a dual-color version of our software. The method of subdivision of objects into groups is also described which is based on color attributes of spots. less

关键词： Enzyme-linked Immunospot Assay (ELISPOT) Image Analysis Spot detection mathematical algorithm Spot recognition

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An Exploratory Study on Optimum Layout of the Cluster Manifold

An Exploratory Study on Optimum Layout of the Cluster Manifo...

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31st ASME International Conference on Ocean, Offshore and Arctic Engineering

作者： Wang, Yingying Duan, Menglan Wang, Deguo Yuan, Xu Tian, Kai China Univ Petr Offshore Oil Gas Res Ctr Beijing Peoples R China

ISBN: (纸本)9780791844922

There are four typical layout types for the subsea production system. These include single well tie back, the daisy chain, the template manifold and the cluster manifold. In the early development phase, an appropriate layout type needs to be selected. The layout design of the subsea production system is based on the data of oil and gas fields. Due to many advantages, such as less initial investment, being installed in advance, flexibility for development schedule and so on, the cluster manifold is becoming more popular and has been applied extensively in the layout of deepwater subsea production systems. In this layout type, the number and locations of cluster manifolds, and the connection types between subsea production facilities have a direct effect on the safety, flexibility and cost of the target deepwater oil and gas fields. Hence, how to design the optimal layout of the cluster manifold is of key importance. This paper will focus on the cluster manifold layout by the math means programmed using C++. For any given subsea well and the floating production system, the basic layout of the cluster manifold with the lowest cost can be obtained based on the assumptions, including the number, locations and cost of the jumpers, PLETS and flowlines. This mathematical method can reduce subjective bias from the engineers and provide a more scientific reference for obtaining the optimal cluster manifold layout.

关键词： Subsea production system Cluster manifold layout mathematical algorithm Calculate software

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Study of Eliminating Calculation Errors in Large-Strain Measurement with Strain Gauges

Study of Eliminating Calculation Errors in Large-Strain Meas...

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2nd International Conference on Applied Mechanics and Mechanical Engineering (ICAMME 2011)

作者： Wang Dengwang Wang Hui Liang Zhigang Tang Shiying Li Yan NW Inst Nucl Technol Xian 710024 Peoples R China

ISBN: (纸本)9783037951217

Resistance strain gauge is common used in strain measurement, particularly in small elastic strain measurement, it has many advantages. However when the measured strain increases, the output nonlinearity of the Wheatstone bridge will become obvious;in this paper the nonlinearity error of the output in large-strain measurement is analyzed, and based on this a mathematical algorithm for nonlinearity compensation is proposed;finally the effect of the algorithm for nonlinearity compensation is proved by theoretical research and experimental treatment.

关键词： large-strain measurement mathematical algorithm nonlinearity compensation

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Study of Eliminating Calculation Errors in Large-Strain Measurement with Strain Gauges

Study of Eliminating Calculation Errors in Large-Strain Meas...

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The 2nd International Conference on Applied Mechanics and Mechanical Engineering(ICAMME 2011)

作者： Wang Dengwang Wang Hui Liang Zhigang Tang Shiying Li Yan Northwest Institute of Nuclear Technology

Resistance strain gauge is common used in strain measurement, particularly in small elastic strain measurement, it has many advantagesHowever when the measured strain increases, the output nonlinearity of the Wheatstone bridge will become obvious;in this paper the nonlinearity error of the output in large-strain measurement is analyzed, and based on this a mathematical algorithm for nonlinearity compensation is proposed;finally the effect of the algorithm for nonlinearity compensation is proved by theoretical research and experimental treatment.

关键词： large-strain measurement mathematical algorithm nonlinearity compensation

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