检索结果-内蒙古大学图书馆

Prenatal microarray comparative genomic hybridization: Experience from the two first years of activity at the Lyon university-hospital

引用

JOURNAL OF GYNECOLOGY OBSTETRICS AND HUMAN REPRODUCTION 2017年第3期46卷 275-283页

作者： Pons, L. Till, M. Alix, E. Abel, C. Boggio, D. Bordes, A. Caloone, J. Raskin, F. C. Chatron, N. Cordier, M. -P. Fichez, A. Labalme, A. Lajeunesse, C. Liaras, E. Massoud, M. Miribel, J. Agnon, E. Schluth-Bolard, C. Vichier-Cerf, A. Edery, P. Attia, J. Huissoud, C. Rudigoz, R. C. Massardier, J. Gaucherand, P. Sanlaville, D. HCL Grp Hosp Est Serv Genet 59 Blvd Pinel F-69677 Bron France Univ Claude Bernard Lyon 1 F-69008 Lyon France HCL Grp Hosp Est Dept Obstet & Gynecol F-69500 Bron France HCL Ctr Hosp Croix Rousse Dept Obstet & Gynecol F-69004 Lyon France HCL Ctr Hosp Lyon Sud Dept Obstet & Gynecol F-69310 Pierre Benite France Ctr Rech Neurosci Lyon INSERM U1028 CNRS UMR 5292 Equipe Gendev F-69500 Bron France

Objectives. - This study aims to describe how microarray comparative genomic hybridization (aCGH) has shifted to become a prenatal diagnosis tool at the Lyon university-hospital. Materials and methods. - This retrospective study included all patients who were referred in the 3 pluridisciplinary centers for prenatal diagnosis of the Lyon university-hospital and who received a prenatal aCGH between June 2013 and June 2015. aCGH was systematically performed in parallel with a karyotype, using the PreCytoNEM array design. Results. - A total of 260 microarrays were performed for the following indications: 249 abnormal ultrasounds (95.8%), 7 characterizations of chromosomal rearrangements (2.7%), and 4 twins with no abnormal ultrasounds (1.5%). With a resolution of 1 mega base, we found 235 normal results (90.4%), 23 abnormal results (8.8%) and 2 non-returns (0.8%). For the chromosomal rearrangements visible on the karyotype, aCGH identified all of the 12 unbalanced rearrangements and did not identify the 2 balanced rearrangements. Among the fetuses with normal karyotypes, 11 showed abnormal microarray results, corresponding to unbalanced cryptic chromosomal rearrangements (4.2%). Conclusion. - Transferring aCGH to a prenatal diagnosis at the Lyon university-hospital has increased the detection rate of chromosomal abnormalities by 4.2% compared to the single karyotype. (C) 2017 Elsevier Masson SAS. All rights reserved.

关键词： Prenatal diagnosis microarray comparative genomic hybridization Abnormal ultrasounds Characterization of chromosomal rearrangements

来源：评论

学校读者我要写书评

暂无评论

microarray comparative genomic hybridization for the Analysis of Bacterial Population Genetics and Evolution

Microarray Comparative Genomic Hybridization for the Analysi...

引用

作者： Caitriona M. Guinane J. Ross Fitzgerald

comparative genomic hybridization analyses have contributed greatly to our understanding of bacterial evolution, population genetics, and pathogenesis. Here, we describe a robust protocol for microarray-based comparis... 详细信息

关键词： genomics microarray comparative genomic hybridization DNA Extraction comparative genomic hybridization microarray hybridization Statistical Calculation microarray Data analysis bacterial evolution population genetics

来源：评论

学校读者我要写书评

暂无评论

Unexpected structural complexity of supernumerary marker chromosomes characterized by microarray comparative genomic hybridization

引用

MOLECULAR CYTOGENETICS 2008年第1期1卷 7-7页

作者： Tsuchiya, Karen D. Opheim, Kent E. Hannibal, Mark C. Hing, Anne V. Glass, Ian A. Raff, Michael L. Norwood, Thomas Torchia, Beth A. Childrens Hosp Dept Labs Seattle WA USA Reg Med Ctr Seattle WA USA Univ Washington Sch Med Dept Lab Med Seattle WA 98195 USA Univ Washington Sch Med Dept Pediat Seattle WA 98195 USA Univ Washington Sch Med Dept Pathol Seattle WA 98195 USA Signature Genom Labs LLC Spokane WA USA

Background: Supernumerary marker chromosomes (SMCs) are structurally abnormal extra chromosomes that cannot be unambiguously identified by conventional banding techniques. In the past, SMCs have been characterized using a variety of different molecular cytogenetic techniques. Although these techniques can sometimes identify the chromosome of origin of SMCs, they are cumbersome to perform and are not available in many clinical cytogenetic laboratories. Furthermore, they cannot precisely determine the region or breakpoints of the chromosome(s) involved. In this study, we describe four patients who possess one or more SMCs (a total of eight SMCs in all four patients) that were characterized by microarray comparative genomic hybridization (array CGH). Results: In at least one SMC from all four patients, array CGH uncovered unexpected complexity, in the form of complex rearrangements, that could have gone undetected using other molecular cytogenetic techniques. Although array CGH accurately defined the chromosome content of all but two minute SMCs, fluorescence in situ hybridization was necessary to determine the structure of the markers. Conclusion: The increasing use of array CGH in clinical cytogenetic laboratories will provide an efficient method for more comprehensive characterization of SMCs. Improved SMC characterization, facilitated by array CGH, will allow for more accurate SMC/phenotype correlation.

关键词： Centromere Probe microarray comparative genomic hybridization Molecular Cytogenetic Technique Peripheral Blood Karyotype Conventional Banding Technique

来源：评论

学校读者我要写书评

暂无评论

microarray-Based comparative genomic hybridization (Array-CGH) as a Useful Tool for Identifying Genes Involved in Glioblastoma (GB)

Microarray-Based Comparative Genomic Hybridization (Array-CG...

引用

作者： Yolanda Ruano Manuela Mollejo Angel Rodríguez Lope José Luis Hernández-Moneo Pedro Martínez Bárbara Meléndez

Alterations in the genome that lead to changes in DNA sequence copy number are a characteristic of Glioblastomas (GBs). microarray-based comparative genomic hybridization (array-CGH) is a high-throughput technology that allows the hybridization of genomic DNA onto conventional cDNA microarrays, normally used in expression profiling, to analyze genomic copy number imbalances. In this way, thousands of genes can be reviewed in a high resolution analysis to define amplicons and to identify? candidate genes showing recurrent genomic copy number changes in GB tumors. less

关键词： microarray microarray comparative genomic hybridization Gene Expression Profiling DNA Extraction Ensembl Homo sapiens Saccharomyces cerevisiae hybridization Amplification genomic DNA Copy number changes Array-CGH GB

来源：评论

学校读者我要写书评

暂无评论

BAC microarray-Based comparative genomic hybridization

BAC Microarray-Based Comparative Genomic Hybridization

引用

作者： Antoine M. Snijders Richard Segraves Stephanie Blackwood Daniel Pinkel Donna G. Albertson

Alterations in DNA copy number underlie certain developmental abnormalities and are frequent in solid tumors. microarray-based comparative genomic hybridization (array CGH) provides a high throughput means to measure and map copy number aberrations on a genome-wide scale and to link them directly to genome sequence. For applications involving the analysis of tumors, the technology must provide reliable detection of single copy gains and losses in mixed cell populations such as tumor and normal cells, accurate quantification of high level copy number gains, and confident interpretation of aberrations affecting only a single array element. Further, one would like to minimize the amount of specimen material required for an analysis. These requirements can be met if there are good signal-to-noise ratios in the hybridization. less

关键词： Ligation-Mediated PCR microarray comparative genomic hybridization Random Primed Labeling Homo sapiens Saccharomyces cerevisiae

来源：评论

学校读者我要写书评

暂无评论

Studying Bacterial Genome Dynamics Using microarray-Based comparative genomic hybridization

Studying Bacterial Genome Dynamics Using Microarray-Based Co...

引用

作者： Eduardo N. Taboada Christian C. Luebbert John H.E. Nash

Genome sequencing has revealed the remarkable amount of genetic diversity that can be encountered in bacterial genomes. In particular, the comparison of genome sequences from closely related strains has uncovered significant differences in gene content, hinting at the dynamic nature of bacterial genomes. The study of these genome dynamics is crucial to leveraging genomic information because the genome sequence of a single bacterial strain may not accurately represent the genome of the species. The dynamic nature of bacterial genome content has required us to apply the concepts of comparative genomics (CG) at the species level. Although direct genome sequence comparisons are an ideal method of performing CG, one current constraint is the limited availability of multiple genome sequences from a given bacterial species. DNA microarray-based comparative genomic hybridization (MCGH), which can be used to determine the presence or absence of thousands of genes in a single hybridization experiment, provides a powerful alternative for determining genome content and has been successfully used to investigate the genome dynamics of a wide number of bacterial species. Although MCGH-based studies have already provided a new vista on bacterial genome diversity, original methods for MCGH have been limited by the absence of novel gene sequences included in the microarray. New applications of the MCGH platform not only promise to accelerate the pace of novel gene discovery but will also help provide an integrated microarray-based approach to the study of bacterial CG. less

关键词： genomics microarray comparative genomic hybridization microarray Gel Electrophoresis Liquid Handling microarray hybridization Statistical Calculation Whole-genome Sequencing PCR Genome Sequencing Data analysis Saccharomyces cerevisiae bacteria genome evolution genomotyping genome content phylogenomics

来源：评论

学校读者我要写书评

暂无评论

Experimental analysis of oligonucleotide microarray design criteria to detect deletions by comparative genomic hybridization

引用

BMC genomicS 2008年第1期9卷 497-497页

作者： Flibotte, Stephane Moerman, Donald G. BC Canc Agcy Canadas Michael Smith Genome Sci Ctr Vancouver BC V5Z 4S6 Canada Univ British Columbia Dept Zool Vancouver BC V6T 1Z4 Canada Univ British Columbia Michael Smith Labs Vancouver BC V6T 1Z4 Canada

Background: microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes. In humans, microarray CGH is widely used to assess copy number variants in healthy individuals and copy number aberrations associated with various diseases, syndromes and disease susceptibility. In model organisms such as Caenorhabditis elegans (C. elegans) the technique has been applied to detect mutations, primarily deletions, in strains of interest. Although various constraints on oligonucleotide properties have been suggested to minimize non-specific hybridization and improve the data quality, there have been few experimental validations for CGH experiments. For genomic regions where strict design filters would limit the coverage it would also be useful to quantify the expected loss in data quality associated with relaxed design criteria. Results: We have quantified the effects of filtering various oligonucleotide properties by measuring the resolving power for detecting deletions in the human and C. elegans genomes using NimbleGen microarrays. Approximately twice as many oligonucleotides are typically required to be affected by a deletion in human DNA samples in order to achieve the same statistical confidence as one would observe for a deletion in C. elegans. Surprisingly, the ability to detect deletions strongly depends on the oligonucleotide 15-mer count, which is defined as the sum of the genomic frequency of all the constituent 15-mers within the oligonucleotide. A similarity level above 80% to non-target sequences over the length of the probe produces significant cross-hybridization. We recommend the use of a fairly large melting temperature window of up to 10 C, the elimination of repeat sequences, the elimination of homopolymers longer than 5 nucleotides, and a threshold of -1 kcal/mol on the oligonucleotide self-folding energy. We observed very little difference in data quality when varying the oli

关键词： comparative genomic hybridization Design Recommendation Standard Filter microarray Design microarray comparative genomic hybridization

来源：评论

学校读者我要写书评

暂无评论

comparative genomic hybridization: DNA Preparation for microarray Fabrication

Comparative Genomic Hybridization: DNA Preparation for Micro...

引用

作者： Richard Redon Diane Rigler Nigel P. Carter

The spatial resolution of microarray-based comparative genomic hybridization (array-CGH) is dependent on the length and density of target DNA sequences covering the chromosomal region of interest. Here we describe the methods developed at the Wellcome Trust Sanger Institute (Cambridge, UK) to construct microarrays comprising large-insert clones available through genome sequencing projects. These methods are applicable to Bacterial and Phage Artificial Chromosomes (BAC and PAC) as well as fosmid and cosmid clones. The protocols are scalable for the construction of microarrays composed of several hundreds up to several ten thousands clones. less

关键词： comparative genomic hybridization Array Printing microarray DNA Extraction PCR microarray comparative genomic hybridization Genome Sequencing Saccharomyces cerevisiae Bos taurus DOP-PCR BAC PAC array-CGH large-insert clones fosmid cosmid

来源：评论

学校读者我要写书评

暂无评论

comparative genomic hybridization by microarray for the Detection of Cytogenetic Imbalance

Comparative Genomic Hybridization by Microarray for the Dete...

引用

作者： Malgorzata Jarmuz Blake C. Ballif Catherine D. Kashork Aaron P. Theisen Bassem A. Bejjani Lisa G. Shaffer

Chromosomal abnormalities often result in the improper dosage of genes in a particular chromosome or chromosome segment, which may cause specific and complex clinical phenotypes. comparative genomic hybridization by microarray (array CGH) is a high-throughput and high-resolution method for the detection of microscopic and submicroscopic chromosome abnormalities, some of which may not be detectable by conventional cytogenetic techniques. In addition, with the human genome sequenced and publicly available, array CGH allows for the direct correlation between chromosomal anomalies and genomic sequence. Properly constructed, microarrays have the potential to be a valuable tool for the detection of chromosomal abnormalities in cancer and genetic disease. less

关键词： microarray comparative genomic hybridization microarray comparative genomic hybridization Cytogenetics Sonication HPLC Homo sapiens DNA copy number chromosomal abnormalities

来源：评论

学校读者我要写书评

暂无评论

comparative genomic hybridization: microarray Design and Data Interpretation

Comparative Genomic Hybridization: Microarray Design and Dat...

引用

作者： Richard Redon Nigel P. Carter

microarray-based comparative genomic hybridization (array-CGH) has been applied for a decade to screen for submicroscopic DNA gains and losses in tumor and constitutional DNA samples. This method has become increasingly flexible with the integration of new biological resources generated by genome sequencing projects. In this chapter, we describe alternative strategies for whole genome screening and high resolution breakpoint mapping of copy number changes by array-CGH, as well as tools available for accurate analysis of array-CGH experiments. Although most methods listed here have been designed for microarrays comprising large-insert clones, they can be adapted easily to other types of microarray platforms, such as those constructed from printed or synthesized oligonucleotides. less

关键词： comparative genomic hybridization microarray microarray comparative genomic hybridization Genome Sequencing CNV calling normalization clone selection Probe design array-CGH outlier detection

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：