检索结果-内蒙古大学图书馆

FSP1: a key regulator of ferroptosis

TRENDS IN MOLECULAR MEDICINE 2023年第9期29卷 753-764页

作者： Li, Wentao Liang, Lin Liu, Siyi Yi, Hong Zhou, Yanhong Cent South Univ Xiangya Hosp Dept Otolaryngol Head & Neck Surg Changsha 410008 Hunan Peoples R China Cent South Univ Xiangya Hosp Res Ctr Carcinogenesis & Targeted Therapy Changsha 410008 Hunan Peoples R China Cent South Univ Xiangya Hosp Natl Clin Res Ctr Geriatr Disorders Changsha 410008 Hunan Peoples R China Cent South Univ Canc Res Inst Basic Sch Med Changsha 410011 Hunan Peoples R China

Ferroptosis suppressor protein 1 (FSP1) is one of the main regulatory molecules of ferroptosis. FSP1 functions through the FSP1-coenzyme Q10 (CoQ10)-NAD(P) H axis and the vitamin K redox cycle. FSP1 is regulated by upstream factors, including transcription factors and noncoding rna (ncrna), and is subject to epigenetic modifications, which affect the progress of FSP1-related diseases. FSP1 is closely associated with the poor prognosis of malignant tumors and plays an important role in disease treatment. This review aims to provide a comprehensive understanding of the role of FSP1 in ferroptosis regulation by summarizing regulatory pathways, possible mechanisms involving FSP1, and the relationship between FSP1 and disease prognosis and treatment.

关键词： FSP1 ferroptosis epigenetic modification noncoding rna regulation mechanism

来源：评论

学校读者我要写书评

暂无评论

Mixed lineage kinase (MLK) controls tumor development and angiogenesis

引用

ANGIOGENESIS 2025年第3期28卷 1-21页

作者： Kant, Shashi Caliz, Amada D. Yoo, Hyung-Jin Kaur, Gaganpreet Learnard, Heather Khalil, Hassan A. Davis, Roger J. Keaney, John F., Jr. Harvard Med Sch Brigham & Womens Hosp Dept Med Div Cardiovasc Med Boston MA 02115 USA Univ Massachusetts Chan Med Sch Dept Med Div Cardiovasc Med Worcester MA 01605 USA Harvard Med Sch Brigham & Womens Hosp Dept Surg Div Div Thorac Surg Boston MA 02115 USA Univ Massachusetts Chan Med Sch Program Mol Med Worcester MA 01605 USA

Cancer is among the leading causes of death in the USA and worldwide. Solid tumors require the formation of new blood vessels (angiogenesis) for their growth. The endothelium plays a crucial role in angiogenesis and tumor progression. Hypoxic stress generated by tumors can activate stress kinases such as mixed lineage kinases (MLKs). Publicly available datasets on lung adenocarcinoma, along with our experimental findings, indicate that MLK2 and MLK3 are expressed in human lung tumors. In this study, using three distinct mouse models of tumor development, we demonstrated that MLK2 (MAP3K10) and MLK3 (MAP3K11) are essential for tumor growth and angiogenesis. Furthermore, MLK2 and MLK3 are highly expressed in the endothelium and are necessary for endothelial proliferation, migration, and angiogenesis. In the endothelium, MLKs regulate the expression of angiogenic growth factors and metalloproteinases, including Pgf, Vegfa, Angptl4, Adam8, and Mmp9. Additionally, the MLK family of kinases acts through the long noncoding rna (lncrna) H19 to control the expression of these pro-angiogenic factors in the endothelium. Collectively, these findings suggest that the MLK-H19 axis coordinates endothelial function, angiogenesis, and tumor growth.

关键词： Angiogenesis Cancer Growth factor Hypoxia noncoding rna Signaling

来源：评论

学校读者我要写书评

暂无评论

Analysis of Interaction Between Long noncoding rnas and Protein by rna Immunoprecipitation in

Analysis of Interaction Between Long Noncoding RNAs and Prot...

引用

作者： Jun Sung Seo Nam-Hai Chua

Long noncoding rnas (lncrnas) play important roles in several processes including control of gene expression. These rnas function through binding to histone-modifying complexes and transcriptional machinery including transcription factor, mediator, and rna polymerase II. We present methods for the discovery and characterization of lncrnas. rna immunoprecipitation (RIP) is a modified version of chromatin immunoprecipitation (ChIP), and it is now generally used in lncrna study. The method allows for testing of lncrna-protein interactions in vivo. RIP assay facilitates the identification of consensus sequences of preferred binding site for the rna-binding protein under study, and identification of the binding sites can provide valuable information on the possible mechanism by which the rna-binding protein functions. less

关键词： ChIP Assay rna Immunoprecipitation (RIP) Cross-linking Immunoprecipitation Reverse Transcription PCR Protein Extraction Sonication Real-time PCR Arabidopsis lncrna noncoding rna rna-protein interaction RIP

来源：评论

学校读者我要写书评

暂无评论

5’tirna-Pro-TGG,a novel trna halve,promotes oncogenesis in sessile serrated lesions and serrated pathway of colorectal cancer

引用

World Journal of Gastrointestinal Oncology 2023年第6期15卷 1005-1018页

作者： Xin-Yuan Wang Yu-Jie Zhou Hai-Ying Chen Jin-Nan Chen Shan-Shan Chen Hui-Min Chen Xiao-Bo Li Division of Gastroenterology and Hepatology Renji HospitalShanghai Jiaotong University School of MedicineShanghai 200000China Department of Spleen and Stomach and Rheumatology Affiliated Traditional Chinese Medicine Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina

BACKGROUND Transfer rna(trna)-derived small rnas(tsrnas)are small fragments that form when trnas *** halves(tirnas),a subcategory of tsrna,are involved in the oncogenic processes of many ***,their specific role in sessile serrated lesions(SSLs),a precancerous lesion often observed in the colon,has not yet been *** To identify SSL-related tirnas and their potential role in the development of SSLs and serrated pathway of colorectal cancer(CRC).METHODS Small-rna sequencing was conducted in paired SSLs and their adjacent normal control(NC)*** expression levels of five SSL-related tirnas were validated by q-polymerase chain *** counting kit-8 and wound healing assays were performed to detect cell proliferation and *** target genes and sites of tirna-1:33-Pro-TGG-1(5′tirna-Pro-TGG)were predicted by TargetScan and miRanda ***-associated and immune-related pathways were analyzed by single-sample gene set enrichment *** analyses were performed to establish the roles of 5′tirna-Pro-TGG based on the target *** In total,we found 52 upregulated tsrnas and 28 downregulated tsrnas in SSLs compared to *** expression levels of tirna-1:33-Gly-CCC-2,tirna-1:33-Pro-TGG-1,and tirna-1:34-Thr-TGT-4-M25′tirnas were higher in SSLs than those in NC,while that of 5′tirna-Pro-TGG was associated with the size of *** was demonstrated that 5′tirnaPro-TGG promoted cell proliferation and migration of RKO cell in ***,heparanase 2(HPSE2)was identified as a potential target gene of 5′*** lower expression was associated with a worse prognosis in ***,lower expression of HPSE2 was observed in SSLs compared to normal controls or conventional adenomas and in BRAF-mutant CRC compared to BRAF-wild *** analyses revealed that its low expression was associated with a low interferonγresponse and also with many metabolic pathways such as riboflavin,retinol,and cy

关键词： noncoding rna trna halves Sessile serrated lesions Colon cancer Serrated pathway

来源：评论

学校读者我要写书评

暂无评论

Evidence that OLE rna is a component of a major stress-responsive ribonucleoprotein particle in extremophilic bacteria

引用

MOLECULAR MICROBIOLOGY 2023年第3期120卷 324-340页

作者： Breaker, Ronald R. Harris, Kimberly A. Lyon, Seth E. Wencker, Freya D. R. Fernando, Chrishan M. Yale Univ Dept Mol Cellular & Dev Biol New Haven CT USA Yale Univ Dept Mol Biophys & Biochem New Haven CT USA Yale Univ Howard Hughes Med Inst New Haven CT USA Yale Univ Dept Mol Cellular & Dev Biol New Haven CT 06511 USA

OLE rna is a similar to 600-nucleotide noncoding rna present in many Gram-positive bacteria that thrive mostly in extreme environments, including elevated temperature, salt, and pH conditions. The precise biochemical functions of this highly conserved rna remain unknown, but it forms a ribonucleoprotein (RNP) complex that localizes to cell membranes. Genetic disruption of the rna or its essential protein partners causes reduced cell growth under various stress conditions. These phenotypes include sensitivity to short-chain alcohols, cold intolerance, reduced growth on sub-optimal carbon sources, and intolerance of even modest concentrations of Mg2+. Thus, many bacterial species appear to employ OLE rna as a component of an intricate RNP apparatus to monitor fundamental cellular processes and make physiological and metabolic adaptations. Herein we hypothesize that the OLE RNP complex is functionally equivalent to the eukaryotic TOR complexes, which integrate signals from various diverse pathways to coordinate processes central to cell growth, replication, and survival.

关键词： cell regulation metabolism noncoding rna ribonucleoprotein complex ribozyme

来源：评论

学校读者我要写书评

暂无评论

Ageing-associated small rna cargo of extracellular vesicles

引用

rna BIOLOGY 2023年第1期20卷 482-494页

作者： Kern, Fabian Kuhn, Thomas Ludwig, Nicole Simon, Martin Groeger, Laura Fabis, Natalie Aparicio-Puerta, Ernesto Salhab, Abdulrahman Fehlmann, Tobias Hahn, Oliver Engel, Annika Wagner, Viktoria Koch, Marcus Winek, Katarzyna Soreq, Hermona Nazarenko, Irina Fuhrmann, Gregor Wyss-Coray, Tony Meese, Eckart Keller, Verena Laschke, Matthias W. Keller, Andreas Saarland Univ Chair Clin Bioinformat Saarland Informat Campus D-66123 Saarbrucken Germany Helmholtz Ctr Infect Res HZI Helmholtz Inst Pharmaceut Res Saarland HIPS Dept Clin Bioinformat Saarbrucken Germany Helmholtz Ctr Infect Res HZI Helmholtz Inst Pharmaceut Res Saarland HIPS Biogen Nanotherapeut Grp ION Saarbrucken Germany Saarland Univ Dept Pharm Saarbrucken Germany Saarland Univ Dept Human Genet Homburg Germany Saarland Univ Ctr Human & Mol Biol Homburg Germany Wuppertal Univ Mol Cell Biol & Microbiol Wuppertal Germany Saarland Univ Dept Genet & Epigenet Saarbrucken Germany Stanford Univ Dept Neurol & Neurol Sci Stanford CA USA INM Leibniz Inst New Mat Saarbrucken Germany Hebrew Univ Jerusalem Edmond & Lily Safra Ctr Brain Sci Jerusalem Israel Univ Freiburg Inst Infect Prevent & Control Fac Med Freiburg Germany Univ Freiburg Med Ctr Freiburg Germany Saarland Univ Inst Clin & Expt Surg Homburg Germany Saarland Univ Ctr Bioinformat Saarbrucken Germany

Previous work on murine models and humans demonstrated global as well as tissue-specific molecular ageing trajectories of rnas. Extracellular vesicles (EVs) are membrane vesicles mediating the horizontal transfer of genetic information between different tissues. We sequenced small regulatory rnas (sncrnas) in two mouse plasma fractions at five time points across the lifespan from 2-18 months: (1) sncrnas that are free-circulating (fc-rna) and (2) sncrnas bound outside or inside EVs (EV-rna). Different sncrna classes exhibit unique ageing patterns that vary between the fcrna and EV-rna fractions. While trnas showed the highest correlation with ageing in both fractions, rrnas exhibited inverse correlation trajectories between the EV- and fc-fractions. For mirnas, the EV-rna fraction was exceptionally strongly associated with ageing, especially the miR-29 family in adipose tissues. Sequencing of sncrnas and coding genes in fat tissue of an independent cohort of aged mice up to 27 months highlighted the pivotal role of miR-29a-3p and miR-29b-3p in ageing-related gene regulation that we validated in a third cohort by RT-qPCR.

关键词： noncoding rna tissue ageing extracellular vesicles plasma mirna >

来源：评论

学校读者我要写书评

暂无评论

Post-transcriptional control of bacterial nitrogen metabolism by regulatory noncoding rnas

引用

WORLD JOUrnaL OF MICROBIOLOGY & BIOTECHNOLOGY 2022年第7期38卷 126-126页

作者： Han, Yueyue Li, Chao Yan, Yongliang Lin, Min Ke, Xiubin Zhang, Yunhua Zhan, Yuhua Chinese Acad Agr Sci Biotechnol Res Inst Beijing Peoples R China Anhui Agr Univ Sch Resources & Environm Hefei Peoples R China

Nitrogen metabolism is the most basic process of material and energy metabolism in living organisms, and processes involving the uptake and use of different nitrogen sources are usually tightly regulated at the transcriptional and post-transcriptional levels. Bacterial regulatory noncoding rnas are novel post-transcriptional regulators that repress or activate the expression of target genes through complementarily pairing with target mrnas;therefore, these noncoding rnas play an important regulatory role in many physiological processes, such as bacterial substance metabolism and stress response. In recent years, a study found that noncoding rnas play a vital role in the post-transcriptional regulation of nitrogen metabolism, which is currently a hot topic in the study of bacterial nitrogen metabolism regulation. In this review, we present an overview of recent advances that increase our understanding on the regulatory roles of bacterial noncoding rnas and describe in detail how noncoding rnas regulate biological nitrogen fixation and nitrogen metabolic engineering. Furthermore, our goal is to lay a theoretical foundation for better understanding the molecular mechanisms in bacteria that are involved in environmental adaptations and metabolically-engineered genetic modifications.

关键词： Nitrogen metabolism Nitrogen fixation noncoding rna Post-transcriptional regulation

来源：评论

学校读者我要写书评

暂无评论

Novel approaches for efficient in vivo fermentation production of noncoding rnas

引用

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2020年第5期104卷 1927-1937页

作者： Yu, Ai-Ming Batra, Neelu Tu, Mei-Juan Sweeney, Colleen Univ Calif Davis Sch Med Dept Biochem & Mol Med Sacramento CA 95817 USA

Genome-derived noncoding rnas (ncrnas), including micrornas (mirnas), small interfering rnas (sirnas), and long noncoding rnas (lncrnas), play an essential role in the control of target gene expression underlying various cellular processes, and dysregulation of ncrnas is involved in the pathogenesis and progression of various diseases in virtually all species including humans. Understanding ncrna biology has opened new avenues to develop novel rna-based therapeutics. Presently, ncrna research and drug development is dominated by the use of ncrna mimics that are synthesized chemically in vitro and supplemented with extensive and various types of artificial modifications and thus may not necessarily recapitulate the properties of natural rnas generated and folded in living cells in vivo. Therefore, there are growing interests in developing novel technologies for in vivo production of rna molecules. The two most recent major breakthroughs in achieving an efficient, large-scale, and cost-effective fermentation production of recombinant or bioengineered rnas (e.g., tens of milligrams from 1 L of bacterial culture) are (1) using stable rna carriers and (2) direct overexpression in rnase III-deficient bacteria, while other approaches offer a low yield (e.g., nano- to microgram scales per liter). In this article, we highlight these novel microbial fermentation-based technologies that have shifted the paradigm to the production of true biological ncrna molecules for research and development.

关键词： noncoding rna Microrna sirna Bioengineering Biotechnology

来源：评论

学校读者我要写书评

暂无评论

Nuclear AGO2 promotes myocardial remodeling by activating ANKRD1 transcription in failing hearts

引用

MOLECULAR THERAPY 2023年第5期32卷 1578-1594页

作者： Xie, Rong Yuan, Shuai Hu, Guo Zhan, Jiabing Jin, Kunying Tang, Yuyan Fan, Jiahui Zhao, Yanru Wang, Feng Chen, Chen Wang, Dao Wen Li, Huaping Huazhong Univ Sci & Technol Tongji Hosp Tongji Med Coll Div Cardiol Wuhan 430030 Peoples R China Hubei Key Lab Genet & Mol Mech Cardiol Disorders Wuhan 430030 Peoples R China Huazhong Univ Sci & Technol Tongji Hosp Tongji Med Coll Dept Internal MedDiv Cardiol 1095 Jiefang Ave Wuhan 430030 Peoples R China

Heart failure (HF) is manifested by transcriptional and posttranscriptional reprogramming of critical genes. Multiple studies have revealed that micrornas could translocate into subcellular organelles such as the nucleus to modify gene expression. However, the functional property of subcellular Argonaute2 (AGO2), the core member of the microrna machinery, has remained elusive in HF. AGO2 was found to be localized in both the cytoplasm and nucleus of cardiomyocytes, and robustly increased in the failing hearts of patients and animal models. We demonstrated that nuclear AGO2 rather than cytosolic AGO2 overexpression by recombinant adeno-associated virus (serotype 9) with cardiomyocyte-specific troponin T promoter exacerbated the cardiac dysfunction in transverse aortic AGO2 activates the transcription of ANKRD1, encoding ankyrin repeat domain-containing protein 1 (ANKRD1), which also has a dual function in the cytoplasm as part of the I-band of the sarcomere and in the nucleus as a transcriptional cofactor. Overexpression of nuclear ANKRD1 recaptured some key features of cardiac remodeling by inducing pathological MYH7 activation, whereas cytosolic ANKRD1 seemed cardioprotective. For clinical practice, we found ivermectin, an antiparasite drug, and ANPep, an ANKRD1 nuclear location signal mimetic peptide, were able to prevent ANKRD1 nuclear import, resulting in the improvement of cardiac performance in TAC-induced HF.

关键词： heart failure nuclear AGO2 noncoding rna transcriptional regulation MYH7/MYH6 ratio

来源：评论

学校读者我要写书评

暂无评论

Development of Ferroptosis-Associated cerna Network in Periodontitis

引用

INTErnaTIONAL DENTAL JOUrnaL 2023年第2期73卷 186-194页

作者： Zhang, Churen Xue, Pengxin Ke, Jianguo Cai, Qiaoling Xiamen Univ Affiliated Hosp 1 Sch Med Dept Stomatol Xiamen Peoples R China Shanghai Jiao Tong Univ People s Hosp 9 Shanghai Peoples R China 55 Zhenhai Ave Xiamen 361003 Peoples R China

Objectives: Periodontitis is a chronic inflammatory illness that may lead to tooth loosening and even loss, and its pathogenesis is not fully understood. Ferroptosis is an iron -depen-dent, regulated cell death. The present study aims to find the key ferroptosis-related genes (FRGs) in periodontitis and develop an mrna-mirna-lncrna network to deeply explore the pathogenesis of periodontitis. Methods: Data from the Gene Expression Omnibus (GEO) database and FerrDb database were downloaded to discover the differentially expressed mrna, mirna, and FRGs. Func-tional enrichment analysis was conducted for the differentially expressed FRGs (DE-FRGs), including gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein-protein interaction (PPI) network analysis. Targetscan and miRtarbase were used to estimate the mirnas that DE-FRGs may interact with, whilst StarBase v3.0 was used for lncrna-mirna ***: Seven DE-FRGs were identified through differential expression analysis. Interleukin 1 beta (IL1B) interacted with XBP1 and MMP13 in the PPI network. After taking the intersec-tion between DE-mirnas and predicted mirnas, a cerna network containing IL1B, has-miR-185, has-miR-204, has-miR-211, has-miR-4306, and 28 lncrnas was established. Conclusions: Seven FRGs in periodontitis were identified, which might promote deeper understanding of ferroptosis in *** 2022 The Authors. Published by Elsevier Inc. on behalf of FDI World Dental Federation. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/)

关键词： Ferroptosis Periodontitis cerna network noncoding rna

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：