检索结果-内蒙古大学图书馆

Circulating long noncoding rna PDE4DIPP6: A novel biomarker for improving the clinical management of non-ST-segment elevation myocardial infarction

引用

CLINICA CHIMICA ACTA 2024年 561卷 119840页

作者： Koch, Pia F. Garcia-Hidalgo, Maria C. Labus, Josephine Biener, Moritz Thum, Thomas de Gonzalo-Calvo, David Baer, Christian Hannover Med Sch Inst Mol & Translat Therapeut Strategies IMTTS Carl Neuberg Str 1 D-30625 Hannover Germany Charite Univ Med Berlin Berlin Germany Free Univ Berlin Berlin Germany Humboldt Univ Dept Surg Expt Surg Berlin Germany Campus Virchow Klinikum Campus Charite Mitte Berlin Germany Univ Hosp Arnau de Vilanova & Santa Maria Translat Res Resp Med IRBLleida Avda Alcalde Rovira Roure 80 Lleida 25198 Spain Inst Hlth Carlos III CIBER Resp Dis CIBERES Madrid Spain Hannover Med Sch Dept Cellular Neurophysiol Hannover Germany Heidelberg Univ Hosp Dept Cardiol Angiol & Pneumol Heidelberg Germany Fraunhofer Inst Toxicol & Expt Med ITEM Hannover Germany

Background: Long noncoding rnas (lncrnas) have emerged as promising diagnostic biomarkers. Here, we investigated the cardiac-expressed and plasma-detectable lncrna PDE4DIPP6 as a biomarker for non-STsegment elevation myocardial infarction (NSTEMI), specifically assessing its potential to enhance the diagnostic efficacy of high-sensitivity cardiac troponin (hs-cTnT). Methods and Results: The study enrolled individuals presenting with suspected acute coronary syndrome (ACS). Lncrna quantification was performed in plasma samples using RT-qPCR. The discriminatory performance was assessed by calculating the Area Under the Curve (AUC). Reclassification metrics, including the Integrated Discrimination Improvement (IDI) and Net Reclassification Improvement (NRI) indexes, were utilized to evaluate enhancements in diagnostic accuracy. Among the 252 patients with suspected ACS, 50.8 % were diagnosed with ACS, and 13.9 % with NSTEMI. Initially, the association of lncrna PDE4DIPP6 with ACS was investigated. Elevated levels of this lncrna were observed in ACS patients compared to non-ACS subjects. No association was found between lncrna PDE4DIPP6 levels and potential confounding factors, nor was a significant correlation with hs-cTnT levels (rho = 0.071). The inclusion of lncrna PDE4DIPP6 on top of hs-cTnT significantly improved the discrimination and classification of ACS patients, as reflected by an enhanced AUC of 0.734, an IDI of 0.066 and NRI of 0.471. Subsequently, the lncrna PDE4DIPP6 was evaluated as biomarker of NSTEMI. Elevated levels of the lncrna were observed in NSTEMI patients compared to patients without NSTEMI. Consistent with previous findings, the addition of lncrna PDE4DIPP6 to hs-cTnT improved the discrimination and classification of patients, increasing the AUC from 0.859 to 0.944, with an IDI of 0.237 and NRI of 0.658. Conclusion: Lncrna PDE4DIPP6 offers additional diagnostic insights beyond hs-cTnT, suggesting its potential to improve the clinical managemen

关键词： Acute Coronary Syndrome Biomarkers Long noncoding rna noncoding rna NSTEMI

来源：评论

学校读者我要写书评

暂无评论

Machine learning for catalysing the integration of noncoding rna in research and clinical practice

引用

EBIOMEDICINE 2024年 106卷 105247-105247页

作者： de Gonzalo-Calvo, David Karaduzovic-Hadziabdic, Kanita Dalgaard, Louise Torp Dieterich, Christoph Perez-Pons, Manel Hatzigeorgiou, Artemis Devaux, Yvan Kararigas, Georgios Univ Hosp Arnau de Vilanova & Santa Maria IRBLleida Translat Res Resp Med Ave Alcalde Rovira Roure 80 Lleida 25198 Spain Inst Hlth Carlos III CIBER Resp Dis CIBERES Madrid Spain Int Univ Sarajevo Fac Engn & Nat Sci Sarajevo Bosnia & Herceg Roskilde Univ Dept Sci & Environm Roskilde Denmark Univ Hosp Heidelberg Klaus Tschira Inst Integrat Computat Cardiol Heidelberg Germany Univ Hosp Heidelberg Dept Internal Med 3 Heidelberg Germany German Ctr Cardiovasc Res DZHK Partner Site Heidelberg Mannheim Germany Univ Thessaly Dept Comp Sci & Biomed Informat DIANA Lab Lamia Greece Hellenic Pasteur Inst Athens Greece Luxembourg Inst Hlth Dept Precis Hlth Cardiovasc Res Unit Strassen Luxembourg Univ Iceland Fac Med Dept Physiol Vatnsmyrarvegur 16 IS-101 Reykjavik Iceland

The human transcriptome predominantly consists of noncoding rnas (ncrnas), transcripts that do not encode proteins. The noncoding transcriptome governs a multitude of pathophysiological processes, offering a rich source of next-generation biomarkers. Toward achieving a holistic view of disease, the integration of these transcripts with clinical records and additional data from omic technologies ("multiomic" " multiomic " strategies) has motivated the adoption of artificial fi cial intelligence (AI) approaches. Given their intricate biological complexity, machine learning (ML) techniques are becoming a key component of ncrna-based research. This article presents an overview of the potential and challenges associated with employing AI/ML-driven approaches to identify clinically relevant ncrna biomarkers and to decipher ncrna-associated pathogenetic mechanisms. Methodological and conceptual constraints are discussed, along with an exploration of ethical considerations inherent to AI applications for healthcare and research. The ultimate goal is to provide a comprehensive examination of the multifaceted landscape of this innovative fi eld and its clinical implications. Copyright (c) 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： Artificial fi cial intelligence Biomarker Machine learning Molecular pathways noncoding rna Personalised medicine

来源：评论

学校读者我要写书评

暂无评论

Exosomal noncoding rna: A potential therapy for retinal vascular diseases

引用

MOLECULAR THERAPY NUCLEIC ACIDS 2024年第1期35卷 102128页

作者： Heo, Jong-Ik Ryu, Juhee Kyungpook Natl Univ Coll Pharm Vessel Organ Interact Res Ctr Daegu South Korea Kyungpook Natl Univ Coll Pharm Daegu South Korea Kyungpook Natl Univ Res Inst Pharmaceut Sci Daegu South Korea

Exosomes are extracellular vesicles that can contain DNA, rna, proteins, and metabolites. They are secreted by cells and play a regulatory role in various biological responses by mediating cell-to-cell communication. Moreover, exosomes are of interest in developing therapies for retinal vascular disorders because they can deliver various substances to cellular targets. According to recent research, exosomes can be used as a strategy for managing retinal vascular diseases, and they are being investigated for therapeutic purposes in eye conditions, including glaucoma, dry eye syndrome, retinal ischemia, diabetic retinopathy, and age-related macular degeneration. However, the role of exosomal noncoding rna in retinal vascular diseases is not fully understood. Here, we reviewed the latest research on the biological role of exosomal noncoding rna in treating retinal vascular diseases. Research has shown that noncoding rnas, including micrornas, circular rnas, and long noncoding rnas play a significant role in the regulation of retinal vascular diseases. Furthermore, through exosome engineering, the expression of relevant noncoding rnas in exosomes can be controlled to regulate retinal vascular diseases. Therefore, this review suggests that exosomal noncoding rna could be considered as a biomarker for diagnosis and as a therapeutic target for treating retinal vascular disease.

关键词： MT: Novel therapeutic targets and biomarker development Special Issue exosomal rna noncoding rna microrna long noncoding rna circular rna retinal vascular disease

来源：评论

学校读者我要写书评

暂无评论

Deciphering chemoresistance in osteosarcoma: Unveiling regulatory mechanisms and function through the lens of noncoding rna

引用

DRUG DEVELOPMENT RESEARCH 2024年第2期85卷 e22167-e22167页

作者： Chen, Hefen Gong, Zhujun Zhou, Hong Han, Yong Huazhong Univ Sci & Technol Union Hosp Tongji Med Coll Dept Pharm Wuhan Peoples R China Chongqing Med Univ Dept Pharm Chongqing Peoples R China Huazhong Univ Sci & Technol Union Hosp Tongji Med Coll Dept Pharm Wuhan 430022 Peoples R China

Osteosarcoma (OS) is a primary malignant bone tumor and is prevalent in children, adolescents, and elderly individuals. It has the characteristics of high invasion and metastasis. Neoadjuvant chemotherapy combined with surgical resection is the most commonly used treatment for OS. However, the efficacy of OS is considerably diminished by chemotherapy resistance. In recent years, noncoding rnas (ncrnas), including micrornas, long noncoding rnas, and circular rnas, are hot topics in the field of chemotherapy resistance research. Several studies have demonstrated that ncrnas are substantially associated with chemoresistance in OS. Thus, the present study overviews the abnormally expressed ncrnas in OS and the molecular mechanisms involved in chemoresistance, with an emphasis on their function in promoting or inhibiting chemoresistance. ncrnas are expected to become potential therapeutic targets for overcoming drug resistance and predictive biomarkers in OS, which are of great significance for enhancing the therapeutic effect and improving the prognosis.

关键词： chemoresistance noncoding rna osteosarcoma

来源：评论

学校读者我要写书评

暂无评论

Death domain-associated protein DAXX regulates noncoding rna transcription at the centromere through the transcription regulator ZFAT

引用

JOUrnaL OF BIOLOGICAL CHEMISTRY 2022年第11期298卷 102528页

作者： Ishikura, Shuhei Yoshida, Kazumasa Tsunoda, Toshiyuki Shirasawa, Senji Fukuoka Univ Fac Med Dept Cell Biol Fukuoka Japan Fukuoka Univ Res Inst Adv Mol Med Fukuoka Japan

The centromere is an essential chromosomal structure for faithful chromosome segregation during cell division. No protein-coding genes exist at the centromeres, but centromeric DNA is actively transcribed into noncoding rna (ncrna). This centromeric transcription and its ncrna products play important roles in centromere functions. We previously re-ported that the transcriptional regulator ZFAT (zinc -finger protein with AT hook) plays a pivotal role in ncrna tran-scription at the centromere;however, it was unclear how ZFAT involvement was regulated. Here, we show that the death domain-associated protein (DAXX) promotes centromeric localization of ZFAT to regulate ncrna transcription at the centromere. Coimmunoprecipitation analysis of endogenous proteins clearly reveals that DAXX interacts with ZFAT. In addition, we show that ectopic coexpression of ZFAT with DAXX increases the centromeric levels of both ZFAT and ncrna, compared with ectopic expression of ZFAT alone. On the other hand, we found that sirna-mediated depletion of DAXX decreases the centromeric levels of both ZFAT and ncrna in cells ectopically expressing ZFAT. These results suggest that DAXX promotes the centromeric localization of ZFAT and ZFAT-regulated centromeric ncrna transcription. Furthermore, we demonstrate that depletion of endogenous DAXX protein is sufficient to cause a decrease in the ncrna levels at the centromeres of chromosomes 17 and X in which ZFAT regulates the transcription, indicating a physiological significance of DAXX in ZFAT-regulated centromeric ncrna transcription. Taken together, these results demonstrate that DAXX regulates centromeric ncrna transcription through ZFAT.

关键词： centromere transcription regulation protein–protein interaction zinc finger DNA-binding protein DAXX noncoding rna ZFAT

来源：评论

学校读者我要写书评

暂无评论

Involvement of noncoding rna in blood-brain barrier integrity in central nervous system disease

引用

NON-CODING rna RESEARCH 2021年第3期6卷 130-138页

作者： Liu, Xi Shen, Ling Han, Bing Yao, Honghong Southeast Univ Sch Med Nanjing 210009 Jiangsu Peoples R China

Given the important role of the blood-brain barrier (BBB) in the central nervous system (CNS), increasing studies have been carried out to determine how the structural and functional integrity of the BBB impacts the pathogenesis of CNS diseases such as stroke, traumatic brain injuries (TBIs), and gliomas. Emerging studies have revealed that noncoding rnas (ncrnas) help to maintain the integrity and permeability of the BBB, thereby mediating CNS homeostasis. This review summarizes recent studies that focus on the effects of ncrnas on the BBB in CNS diseases, including regulating the biological processes of inflammation, necrosis, and apoptosis of cells, affecting the translational dysfunction of proteins and regulating tight junctions (TJs). A comprehensive and detailed understanding of the interaction between ncrnas and the BBB will lay a solid foundation for the development of early diagnostic methods and effective treatments for CNS diseases.

关键词： noncoding rna Blood-brain barrier Central nervous system disease

来源：评论

学校读者我要写书评

暂无评论

Functions, mechanisms, and therapeutic implications of noncoding rna in acute myeloid leukemia

引用

Fundamental Research 2024年

作者： Wang, Xiaokang Tong, Yong Xun, Tianrong Feng, Haixing Lei, Yuhe Li, Yuanqing Wu, Kit Hang Qiu, Fang Department of Pharmacy Shenzhen Longhua District Central Hospital Shenzhen 518110 China Department of Pharmacy Shenzhen Hospital Southern Medical University Shenzhen 518000 China Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs School of Pharmacy Guangdong Medical University Dongguan 523109 China The Marine Biomedical Research Institute of Guangdong Zhanjiang Zhanjiang 524044 China Department of Hematology Shenzhen Hospital Southern Medical University Shenzhen 518000 China Department of Neurology Shenzhen Institute of Translational Medicine The First Affiliated Hospital of Shenzhen University Shenzhen Second People's Hospital Shenzhen 518025 China Department of Pharmacy Shenzhen Hospital of Guangzhou University of Chinese Medicine Shenzhen 518034 China Department of Pharmacy Nossa Senhora do Carmo - Lago Health Centre Health Bureau Macau SAR 999078 China Department of Burn and Plastic Surgery Shenzhen Longhua District Central Hospital Affiliated Central Hospital of Shenzhen Longhua District Guangdong Medical University Shenzhen 518110 China

Acute myeloid leukaemia (AML) is a malignant disease of myeloid hematopoietic stem/progenitor cells. Despite improved understanding of the pathogenesis of AML since the 1980s, the standard treatment for AML has remained virtually unchanged. Numerous studies have found poor survival rates and high relapse rates among older patients with AML. Several novel therapies for AML, including cytotoxic drugs, genetically- and epigenetically-targeted drugs, and immunotherapies, have been developed in recent years. Alternative treatments with improved efficacy are required for AML because many patients cannot tolerate the toxic effects of chemotherapy. Non-coding rnas, including micrornas (mirnas), long noncoding rnas (lncrnas) and circular rnas (circrnas), are attractive treatment targets for cancers and several other diseases. Lncrnas, mirnas and circrnas regulate DNA transcription and translation. Over the past decade, significant efforts have been made to develop rna-based therapies, mainly antisense oligonucleotides and small interfering rna, some of which have been approved for clinical use. Here we reviewed the mechanisms underlying the in vitro and in vivo effects of promising targets and potential drugs, focusing on the drugs most likely to be used for clinical treatment, to aid in the development of precision therapy for AML. © 2023 The Authors

关键词： Acute myeloid leukaemia Chemoresistance Molecular targeted agents noncoding rna Small molecule drugs

来源：评论

学校读者我要写书评

暂无评论

Pregnancy-associated changes in cervical noncoding rna

引用

EPIGENOMICS 2020年第12期12卷 1013-1025页

作者： Gerson, Kristin D. Haviland, Miriam J. Neo, Dayna Hecht, Jonathan L. Baccarelli, Andrea A. Brennan, Kasey J. M. Dereix, Alexandra E. Ralston, Steven J. Hacker, Michele R. Burris, Heather H. Univ Penn Maternal Child Hlth Res Ctr Ctr Res Reprod & Womens Hlth Dept Obstet & GynecolPerelman Sch Med Philadelphia PA 19104 USA Beth Israel Deaconess Med Ctr Dept Obstet & Gynecol Boston MA 02215 USA Beth Israel Deaconess Med Ctr Dept Pathol Boston MA 02215 USA Harvard Med Sch Dept Pathol Boston MA 02115 USA Columbia Univ Dept Environm Hlth Mailman Sch Publ Hlth New York NY 10032 USA Penn Hosp Dept Obstet & Gynecol Philadelphia PA 19107 USA Univ Penn Perelman Sch Med Dept Obstet & Gynecol Philadelphia PA 19104 USA Harvard Med Sch Dept Obstet Gynecol & Reprod Biol Boston MA 02115 USA Harvard TH Chan Sch Publ Hlth Dept Epidemiol Boston MA 02115 USA Childrens Hosp Philadelphia Dept Pediat Philadelphia PA 19104 USA Univ Penn Perelman Sch Med Dept Pediat Philadelphia PA 19104 USA Beth Israel Deaconess Med Ctr Dept Neonatol Boston MA 02215 USA Univ Penn Maternal Child Hlth Res Ctr Ctr Res Reprod & Womens Hlth Perelman Sch Med Philadelphia PA 19104 USA

Aim:To identify pregnancy-associated changes in cervical noncoding rna (ncrna), including mirna and long noncoding rna (lncrna), and their potential effects on biologic *** & methods:We enrolled 21 pregnant women with term deliveries (>= 37 weeks' gestation) in a prospective cohort and collected cervical swabs before 28 weeks' gestation. We enrolled 21 nonpregnant controls. We analyzed mirna, lncrna and mrna expression, applying a Bonferroni ***:Five mirna and three lncrna were significantly differentially (>twofold change) expressed. Putative mirna targets are enriched in genes mediating organogenesis, glucocorticoid signaling, cell adhesion and ncrna ***:Differential cervical ncrna expression occurs in the setting of pregnancy. Gene ontology classification reveals biological pathways through which mirna may play a biologic role in normal pregnancy physiology.

关键词： cervix epigenetics gene ontology long noncoding rna microrna noncoding rna pregnancy

来源：评论

学校读者我要写书评

暂无评论

Salivary noncoding rna in the diagnosis of pancreatic cancer: Systematic review and meta-analysis

引用

EUROPEAN JOUrnaL OF CLINICAL INVESTIGATION 2022年第12期52卷 e13848-e13848页

作者： Koopaie, Maryam Kolahdooz, Sajad Fatahzadeh, Mahnaz Aleedawi, Zainab Abdulkareem Univ Tehran Med Sci Tehran Iran Univ Tehran Med Sci USERN Tehran Iran Rutgers Sch Dent Med Dept Diagnost Sci Newark NJ USA Univ Tehran Med Sci Sch Dent North Kargar StPOB 14395 433 Tehran *** Iran Private Dent Clin Beirut Lebanon

Background Pancreatic cancer is considered one of the most deadly malignancies, primarily because of its diagnostic challenges. We performed a systematic review and diagnostic meta-analysis to evaluate the diagnostic value of noncoding salivary rnas in pancreatic cancer diagnosis. Methods Our investigation involved pertinent studies published in PubMed, Scopus, Web of Science, LIVIVO, Ovid and also the Google Scholar search engine. Specificity and sensitivity were calculated, as were positive and negative likelihood ratios (PLR and NLR), and the diagnostic odds ratio (DOR). The summary receiver-operating characteristics and area under the curve were plotted and assessed. Results This meta-analysis and systematic review involved and examined five studies that contained 145 study units with a total of 2731 subjects (1465 pancreatic cancer patients versus 1266 noncancer controls). The pooled specificity, sensitivity, NLR, PLR and DOR were 0.783 (95% CI: 0.759-0.805), 0.829 (95% CI: 0.809-0.848), 0.309 (95% CI: 0.279-0.343), 3.386 (95% CI: 2.956-3.879) and 18.403 (95% CI: 14.753-22.954), respectively, with the area under the curve (AUC) equal to 0.882. Subgroup analyses were conducted based on the saliva type (unstimulated and stimulated), mean age of patients, sample size, type of control, serum carbohydrate antigen 19-9 (CA19-9) level and type of salivary noncoding rna (microrna (mirna) and long noncoding rna (lncrna)). Conclusions The results of our systematic review and meta-analysis suggest that noncoding rna biomarkers in the stimulated saliva could be a promising approach for accurate pancreatic cancer diagnosis in the early stages.

关键词： biomarker diagnosis meta-analysis noncoding rna pancreatic cancer saliva

来源：评论

学校读者我要写书评

暂无评论

A Polymorphism in the Epstein-Barr Virus EBER2 noncoding rna Drives In Vivo Expansion of Latently Infected B Cells

引用

MBIO 2022年第3期13卷 e0083622页

作者： Wang, Yiping Ungerleider, Nathan Hoffman, Brett A. Kara, Mehmet Farrell, Paul J. Flemington, Erik K. Lee, Nara Tibbetts, Scott A. Univ Florida Coll Med UF Genet Inst Dept Mol Genet & MicrobiolUF Hlth Canc Ctr Gainesville FL USA Tulane Univ Sch Med Tulane Canc Ctr Dept Pathol 1430 Tulane Ave New Orleans LA 70112 USA Imperial Coll London Dept Infect Dis London England Univ Pittsburgh Sch Med Dept Microbiol & Mol Genet Pittsburgh PA USA

The oncogenic gammaherpesviruses, including human Epstein-Barr virus (EBV), human Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, gamma HV68, MuHV-4), are associated with numerous malignancies, including B cell lymphomas and nasopharyngeal carcinoma. These viruses employ numerous molecular strategies to colonize the host, including the expression of noncoding rnas (ncrnas). As the first viral ncrnas identified, EBV-encoded rna 1 and 2 (EBER1 and EBER2, respectively) have been investigated extensively for decades;however, their specific in vivo functions remain largely unknown. In work here, we used chimeric MHV68 viruses in an in vivo complementation system to test whether EBV EBER2 contributes to acute and/or chronic phases of infection. Expression of EBER2 derived from EBV strain B95-8 resulted in a significant expansion of latently infected B cells in vivo, which was accompanied by a decrease in virus-infected plasma cells. EBV strains typically carry one of two variants of EBER2, which differ primarily by a 5-nucleotide core polymorphism identified initially in the EBV strain M81. Strikingly, mutation of the 5 nucleotides that define this core polymorphism resulted in the loss of the infected B cell expansion and restored plasma cell infection. This work reveals that the B95-8 variant of EBER2 promotes the expansion of the latently infected B cell pool in vivo and may do so in part through inhibition of terminal differentiation. These findings provide new insight into mechanisms by which viral ncrnas promote in vivo colonization and further and provide further evidence of the inherent tumorigenic risks associated with gammaherpesvirus manipulation of B cell differentiation. IMPORTANCE The oncogenic gammaherpesviruses, including human Epstein-Barr virus (EBV), human Kaposi's sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68, employ numerous strategies to colonize the host, including expression of noncodin

关键词： EBV Epstein-Barr virus EBER MHV68 noncoding rna B lymphocytes gammaherpesviruses latency lymphoma

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：