检索结果-内蒙古大学图书馆

Cumulus cell microRNA expression when LH is added to the ovarian stimulation protocol: a pilot study

REPRODUCTIVE BIOMEDICINE ONLINE 2021年第6期43卷 1070-1077页

作者： Vigo, Francieli Maria Fraietta, Renato Rodrigues, Fernanda Carvalho, Cristina, V Bonetti, Tatiana C. S. Motta, Eduardo L. A. Univ Fed Sao Paulo Dept Ginecol Escola Paulista Med UNIFESP EPM Rua Napoleao Barros608 Vila Clementino BR-04024002 Sao Paulo SP Brazil Univ Fed Sao Paulo Setor Integrad Reprod Humana UNIFESP EPM Escola Paulista MedDept Cirurgia Rua Botucatu725 Vila Vila Clementino BR-04024002 Sao Paulo SP Brazil Huntington Med Reprod Av Republ Libano 529 Ibirapuera BR-04501000 Sao Paulo SP Brazil

Research question: Recombinant FSH administration in ovarian stimulation for IVF is a standard procedure, whereas the role of LH is controversial. MicroRNAs (mRNA) are small endogenous non-coding transcripts that are involved in the regulation of many cellular processes, including foliculogenesis and gonadotrophin function. The aim was to study the possible role of miRNA in ovarian follicular development in groups having different ovarian stimulation protocols. Are there different miRNA expression profiles in cumulus cells of infertile women undergoing IVF? What are the regulated pathways? Design: This prospective observational study included 13 patients who fulfilled the following inclusion criteria: younger than 38 years of age;a tubal infertility factor;a male factor;or idiopathic infertility. This is a pilot study in which the patients were aleatory enrolled into two groups: seven in FSH group (recombinant FSH, 225 IU) and six in FSH plus LH group (recombinant FSH, 150 IU + recombinant LH, 75 IU). The granulosa cells obtained from the follicular ovarian retrieval were analysed using polyerase chain reaction. Results were analysed using DIANA Tools, an online bioinformatics tool. Results: Among the 84 microRNAs evaluated, 11 were differentially expressed between the groups, all of which were upregulated in the FSH plus LH group, compared with the FSH group. Differentially expressed miRNA profiles are related to oestrogen signalling, oocyte meiosis and pluripotent cells regulation. Conclusion: miRNA overexpression in the FSH plus LH group is consistent with the independent and fundamental role of LH in folliculogenesis, leading to a distinct molecular response between groups.

关键词： MicroRNA Ovarian stimulation pcr array Recombinant LH

来源：评论

学校读者我要写书评

暂无评论

Differential gene expressions of the MAPK signaling pathway in enterovirus 71-infected rhabdomyosarcoma cells

引用

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES 2013年第4期17卷 410-417页

作者： Shi, Weifeng Hou, Xueling Li, Xiang Peng, Hongjun Shi, Mei Jiang, Qingbo Liu, Xiping Ji, Yun Yao, Yuhua He, Caizhen Lei, Xiangdong Suzhou Univ Affiliated Hosp 3 Dept Clin Lab Changzhou Jiangsu Peoples R China CT Biosci Inst Chang Zhou Jiangsu Peoples R China

Background: Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in response to viral infection. The aim of this study was to explore the function and mechanism of MAPK signaling pathway in enterovirus 71 (EV71) infection of human rhabdomyosarcoma (RD) cells. Methods: Apoptosis of RD cells was observed using annexin V-FITC/PI binding assay under a fluorescence microscope. Cellular RNA was extracted and transcribed to cDNA. The expressions of 56 genes of MAPK signaling pathway in EV71-infected RD cells at 8 h and 20 h after infection were analyzed by pcr array. The levels of IL-2, IL-4, IL-10, and TNF-alpha in the supernatant of RD cells infected with EV71 at different time points were measured by ELISA. Results: The viability of RD cells decreased obviously within 48 h after EV71 infection. Compared with the control group, EV71 infection resulted in the significantly enhanced releases of IL-2, IL-4, IL-10 and TNF-alpha from infected RD cells (p < 0.05). At 8 h after infection, the expressions of c-Jun, c-Fos, IFN-beta, MEKK1, MLK3 and NIK genes in EV71-infected RD cells were up-regulated by 2.08-6.12-fold, whereas other 19 genes (e.g. AKT1, AKT2, E2F1, IKK and NF-kappa B1) exhibited down-regulation. However, at 20 h after infection, those MAPK signaling molecules including MEKK1, ASK1, MLK2, MLK3, NIK, MEK1, MEK2, MEK4, MEK7, ERK1, JNK1 and JNK2 were up-regulated. In addition, the expressions of AKT2, ELK1, c-Jun, c-Fos, NF-kappa B p65, PI3K and STAT1 were also increased. Conclusion: EV71 infection induces the differential gene expressions of MAPK signaling pathway such as ERK, JNK and PI3K/AKT in RD cells, which may be associated with the secretions of inflammatory cytokines and host cell apoptosis. (c) 2013 Elsevier Editora Ltda. All rights reserved.

关键词： Enterovirus 71 Rhabdomyosarcoma cells MAPK pcr array

来源：评论

学校读者我要写书评

暂无评论

Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves

引用

PARASITOLOGY RESEARCH 2018年第10期117卷 3269-3276页

作者： Yin, Fangyuan Liu, Junlong Gao, Shandian Liu, Aihong Zhao, Shuaiyang Li, Sitong Wang, Jinming Li, Youquan Luo, Jianxun Guan, Guiquan Yin, Hong Chinese Acad Agr Sci Lanzhou Vet Res Inst Key Lab Vet Parasitol Gansu Prov State Key Lab Vet Etiol Biol Xujiaping 1 Lanzhou 730046 Gansu Peoples R China Jiangsu Coinnovat Ctr Prevent & Control Important Yangzhou 225009 Jiangsu Peoples R China

Theileria annulata is the pathogen of bovine tropical theileriosis. It is extremely harmful to the cattle industry, with huge economic losses. The toll-like receptor (TLR) and NOD-like receptor (NLR) signaling pathways are crucial for resistance to infection of the protozoa, such as Plasmodium falciparum, Toxoplasma gondii, and Trypanosoma cruzi. However, the role of these immune-related pathways is unclear during T. annulata infection. In the present study, peripheral blood mononuclear cells and serum were separated from blood samples of calves infected with homogenized tick supernatants carrying T. annulata sporozoites at 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h postinoculation. The Custom RT2 Profiler pcr array was used to explore the mRNA levels of 42 TLR and NLR signaling pathway relevant genes. The TLR1, TLR6, TLR10, NLRP1, and MyD88 genes and their downstream signaling molecules significantly differed after the T. annulata infection in comparison with that of preinfection from 72 h to 168 h postinoculation. The serum concentrations of IL-6, IL-1 beta, and TNF alpha were significantly increased at 96 h and 168 h postinfection. These findings provided novel information to help determine the mechanisms of TLR and NLR signaling pathway involvement in protection against T. annulata infection.

关键词： Theileria annulata Innate immunity TLRs NLRs pcr array

来源：评论

学校读者我要写书评

暂无评论

Chemokine CXCL1 may serve as a potential molecular target for hepatocellular carcinoma

引用

CANCER MEDICINE 2016年第10期5卷 2861-2872页

作者： Han, Ke-qi Han, Hui He, Xue-qun Wang, Lei Guo, Xiao-dong Zhang, Xue-ming Chen, Jie Zhu, Quan-gang Nian, Hua Zhai, Xiao-feng Jiang, Ma-wei Shanghai Univ Tradit Chinese Med Shanghai Yueyang Hosp Integrated Tradit Chinese & Dept Oncol & Pharm Shanghai 200437 Peoples R China Second Mil Med Univ Changhai Hosp Dept Tradit Chinese Med Shanghai 200433 Peoples R China Shanghai Jiao Tong Univ Xin Hua Hosp Sch Med Dept Oncol Kongjiang Rd 1665 Shanghai 200092 Peoples R China

The purpose of this study was to screen for changes in chemokine and chemokine-related genes that are expressed in hepatocellular carcinoma (HCC) as potential markers of HCC progression. Total RNA was extracted from tumor and peri-tumor tissues from mice with HCC and analyzed using a pcr microarray comprising 98 genes. Changes in gene expression of threefold or more were screened and subsequently confirmed by immunohistochemical analyses and western blotting. Furthermore, whether chemokine knockdown by RNA interference (RNAi) could significantly suppress tumor growth in vivo was also evaluated. Finally, total serum samples were collected from HCC patients with HBV/cirrhosis (n = 16) or liver cirrhosis (n = 16) and from healthy controls (n = 16). The serum mRNA and protein expression levels of CXCL1 in primary liver cancer patients were detected by qRT-pcr and western blot analysis, respectively. Several genes were up-regulated in tumor tissues during the progression period, including CXCL1, CXCL2, CXCL3, and IL-1 beta, while CXCR1 expression was down-regulated. CBRH-7919 cells carrying CXCL1 siRNA resulted in decreased tumor growth in nude mice. The differences in serum CXCL1 mRNA and protein levels among the HCC, hepatic sclerosis (HS), and control groups were significant (P < 0.001). The mRNA and protein levels of CXCL1 in the HCC group were up-regulated compared with the HS group or the control group (P < 0.001). Several chemokine genes were identified that might play important roles in the tumor microenvironment of HCC. These results provide new insights into - human HCC and may ultimately facilitate early HCC diagnosis and lead to the discovery of innovative therapeutic approaches for HCC.

关键词： Chemokines gene expression profile hepatocellular carcinoma pcr array siRNA

来源：评论

学校读者我要写书评

暂无评论

Integrated analysis of microRNAs and their disease related targets in the brain of mice infected with West Nile virus

引用

VIROLOGY 2014年 452卷 143-151页

作者： Kumar, Mukesh Nerurkar, Vivek R. Univ Hawaii Manoa John A Burns Sch Med Dept Trop Med Med Microbiol & Pharmacol Honolulu HI 96813 USA Univ Hawaii Manoa John A Burns Sch Med Pacific Ctr Emerging Infect Dis Res Honolulu HI 96813 USA

To determine whether cellular miRNAs play a role in West Nile virus (WNV) neuropathogenesis, we evaluated WNV-infected mice brain for the expression profile of miRNAs, their potential functions and their correlation with genes involved in inflammatory pathways. A total of 528 miRNAs and 168 mRNA genes were examined. One hundred thirty-nine miRNAs were significantly differentially expressed in WNV-infected mice brain. Ingenuity pathway analysis demonstrated that these miRNAs and their target genes are involved in pathways related to inflammatory response, immune-cell trafficking and cell death. Moreover, we demonstrate an inverse correlation between WNV-modulated miRNAs and their target neuroinflammatory genes in the same mice brain. We demonstrate that miR-196a, miR-202-3p, miR-449c, and miR-125a-3p target multiple genes involving cytokines, chemokines, and apoptotic genes, which belong to different signaling pathways that play critical role in WNV neuropathogenesis. Functional studies targeting specific miRNA are warranted to develop therapeutics for the management of WNV disease. (C) 2014 Elsevier Inc. All rights reserved.

关键词： West Nile virus Flavivirus miRNA pcr array Neuroinflammation miR-196a miR-202-3p miR-449c miR-125a-3p

来源：评论

学校读者我要写书评

暂无评论

Selection and validation of reliable reference genes for RT-qpcr analysis in a large cohort of pituitary adenomas

引用

MOLECULAR AND CELLULAR ENDOCRINOLOGY 2016年第C期437卷 183-189页

作者： Normann, Kjersti Ringvoll Oystese, Kristin Astrid Berland Berg, Jens Petter Lekva, Tove Berg-Johnsen, Jon Bollerslev, Jens Olarescu, Nicoleta Cristina Oslo Univ Hosp Dept Endocrinol Sect Specialized Endocrinol Oslo Norway Univ Oslo Fac Med Inst Clin Med Oslo Norway Oslo Univ Hosp Internal Med Res Inst Oslo Norway Oslo Univ Hosp Dept Med Biochem Oslo Norway Oslo Univ Hosp Dept Neurosurg Oslo Norway

Background: Real-time reverse transcription quantitative pcr (RT-qpcr) has become the method of choice for quantification of gene expression changes. The most important limitations of RT-qpcr are inappropriate data normalization and inconsistent data analyses. Pituitary adenomas are common tumours, and the appropriate interpretation of increasingly published data within this field is prevented by the lack of a proper selection and validation of stably expressed reference genes. Aim: To find and validate the optimal reference gene or gene combination for reliable RT-qpcr gene expression in both non-functioning (NFPA) and hormone secreting (GH and ACTH) pituitary adenomas. Material and methods: Thirty commonly used reference genes (pcr array reference gene panel, BioRad, Hercules, CA) were quantified by RT-qpcr in 24 pituitary adenomas (12 NFPA, 8 GH and 4 ACTH). The data was analysed using three programs: geNorm (Qbase+), Normfinder and BestKeeper having different algorithms to identify the most stable reference gene or combination of reference genes. Three reference genes ALAS1, PSMC4 and GAPDH, were selected for further validation in a larger cohort of 223 adenomas (141 NFPA, 63 GH and 19 ACTH). Results: In all adenomas, ALAS1 and PSMC4 were the most stable reference genes as estimated by geNorm and Normfinder, whereas Bestkeeper ranked RPLP0 and ACTS as the two most stable out of 10 carefully selected genes. The best gene combination was PSMC4 and ALAS1 (geNorm) or PSMC4 and GAPDH (Normfinder). The validation experiment (geNorm) showed that the most stable gene combinations were ALAS1 and GAPDH in NFPA, and PSMC4 and GAPDH in hormone secreting adenomas. Conclusions: Several of the reference genes expressed good stability yielding several candidate genes. PSMC4 and ALAS1 were overall the most stably expressed genes in pituitary adenoma merely differing in ranking order. PSMC4 and ALAS1 have so far not been reported as reference genes in pituitary adenomas. The vari

关键词： Non-functioning pituitary adenoma (NFPA) pcr array Gene expression geNorm NormFinder BestKeeper MIQE guidelines

来源：评论

学校读者我要写书评

暂无评论

Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells

引用

JOURNAL OF APPLIED PHYSIOLOGY 2011年第4期111卷 1072-1079页

作者： Wenger, Karl H. El-Awady, Ahmed R. Messer, Regina L. W. Sharawy, Mohamed M. White, Greg Lapp, Carol A. Georgia Hlth Sci Univ Dept Orthopaed Surg Augusta GA 30912 USA Med Coll Georgia Dept Oral Biol Augusta GA 30912 USA Med Coll Georgia Operat Off Augusta GA 30912 USA

Wenger KH, El-Awady AR, Messer RL, Sharawy MM, White G, Lapp CA. Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells. J Appl Physiol 111: 1072-1079, 2011. First published July 14, 2011;doi:10.1152/japplphysiol.01175.2010.-A bioreactor system was developed to provide high-amplitude cyclic hydrostatic compressive stress (cHSC) using compressed air mixed commercially as needed to create partial pressures of oxygen and carbon dioxide appropriate for the cells under investigation. Operating pressures as high as 300 psi are achievable in this system at cyclic speeds of up to 0.2 Hz. In this study, ligamentous fibroblasts from human periodontal ligaments (n = 6) were compressed on two consecutive days at 150 psi for 3 h each day, and the mRNA for families of extracellular matrix protein and protease isoforms was evaluated by real-time pcr array. Several integrins were significantly upregulated, most notably alpha-3 (6.4-fold), as was SPG7 (12.1-fold). Among the collagens, Col8a1 was highly upregulated at 53.5-fold, with Col6a1, Col6a2, and Col7a1 also significantly upregulated 4.4-to 8.5-fold. MMP-1 was the most affected at 122.9-fold upregulation. MMP-14 likewise increased 17.8-fold with slight reductions for the gelatinases and a significant increase of TIMP-2 at 5.8-fold. The development of this bioreactor system and its utility in characterizing periodontal ligament fibroblast mechanobiology in intermediate-term testing hold promise for better simulating the conditions of the musculoskeletal system and the large cyclic compressive stresses joints may experience in gait, exertion, and mastication.

关键词： compression fibroblast gene expression extracellular matrix protease cell adhesion real-time pcr pcr array

来源：评论

学校读者我要写书评

暂无评论

Osteogenic Potential of the Transcription Factor c-MYB

引用

CALCIFIED TISSUE INTERNATIONAL 2017年第3期100卷 311-322页

作者： Oralova, V. Matalova, E. Killinger, M. Knopfova, L. Smarda, J. Buchtova, M. Czech Acad Sci Inst Anim Physiol & Genet Vvi Veveri 97 Brno 60200 Czech Republic Univ Ghent Evolutionary Dev Biol Ghent Belgium Univ Vet & Pharmaceut Sci Dept Physiol Brno Czech Republic Masaryk Univ Dept Expt Biol Brno Czech Republic St Annes Univ Hosp Int Clin Res Ctr Brno Czech Republic

The transcription factor c-MYB is a well-known marker of undifferentiated cells such as haematopoietic cell precursors, but recently it has also been observed in differentiated cells that produce hard tissues. Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in c-myb-overexpressing cultures, whereas downregulation of c-myb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.

关键词： Mineralised matrix Micromass cultures Mouse limbs Osteogenesis pcr array

来源：评论

学校读者我要写书评

暂无评论

HMGB1 Osteo-Modulatory Action on Osteosarcoma SaOS-2 Cell Line: An Integrated Study From Biochemical and -Omics Approaches

引用

JOURNAL OF CELLULAR BIOCHEMISTRY 2016年第11期117卷 2559-2569页

作者： Martinotti, Simona Patrone, Mauro Manfredi, Marcello Gosetti, Fabio Pedrazzi, Marco Marengo, Emilio Ranzato, Elia Univ Piemonte Orientale DiSIT Dipartimento Sci & Innovaz Tecnol Viale Teresa Michel 11 I-15121 Alessandria Italy Isalit Srl Sede Legale Via Bovio 6 Novara Italy Politecn Torino Sede Operat Sede Alessandria Viale Teresa Michel 5 I-15121 Alessandria Italy Univ Genoa Biochem Sect Dept Expt Med DIMES Viale Benedetto 15 1 I-16132 Genoa Italy

High mobility group box protein-1 (HMGB1) is released from cells under various pathological conditions and it plays a pivotal role as an alarmin signaling tissue damage. Little is known about the impact of HMGB1 in bone repair and remodeling. To this aim, we focused on HMGB1-induced effects on the in vitro osteoblast model SaOS-2. Cell proliferation was stimulated with a maximum at concentration of 2.5nM, and such a dose also stimulated cell migration and scratch wound healing. We then characterized the modulatory effect of HMGB1 on bone biology, by using osteogenesis/mineralization assays, a pcr array, and the analysis of a series of osteogenic markers. We performed also a proteomic screening using SWATH-MS on SaOS-2 cell exposed to HMGB1 and we provide evidence for proteins modulated in HMGB1 exposed cells. Taken together, our data demonstrate that SaOS-2 cell proliferation, migration, and osteogenic differentiation were increased by HMGB1. We, therefore, propose that HMGB1 could be a potent bone-remodeling signal but the physiological meaning of this property remains to be more ascertained. J. Cell. Biochem. 117: 2559-2569, 2016. (c) 2016 Wiley Periodicals, Inc.

关键词： HMGB1 SaOS-2 OSTEOGENESIS SCRATCH WOUND ASSAY MIGRATION pcr array SWATH-MS

来源：评论

学校读者我要写书评

暂无评论

Cytotoxicity of trans-chalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest

引用

BIOMEDICINE & PHARMACOTHERAPY 2017年 85卷 425-433页

作者： Buso Bortolotto, Luis Felipe Barbosa, Flavia Regina Silva, Gabriel Bitencourt, Tamires Aparecida Beleboni, Rene Oliveira Baek, Seung Joon Marins, Mozart Fachin, Ana Lucia Univ Ribeirao Preto Biotechnol Unit Av Costabile Romano 2201 BR-14096900 Ribeirao Preto SP Brazil Univ Tennessee Coll Vet Med Dept Biomed & Diagnost Sci Knoxville TN USA

Chalcones are precursors of flavonoids that exhibit structural heterogeneity and potential antitumor activity. The objective of this study was to characterize the cytotoxicity of trans-chalcone and licochalcone A (LicoA(1)) against a breast cancer cell line (MCF-7) and normal murine fibroblasts (3T3). Also the mechanisms of the anti-cancer activity of these two compounds were studied. The alkaline comet assay revealed dose-dependent genotoxicity, which was more responsive against the tumor cell line, compared to the 3T3 mouse fibroblast cell line. Flow cytometry showed that the two chalcones caused the cell cycle arrest in the G1 phase and induced apoptosis in MCF-7 cells. Using pcr array, we found that trans-chalcone and LicoA trigger apoptosis mediated by the intrinsic pathway as demonstrated by the inhibition of Bcl-2 and induction of Bax. In western blot assay, the two chalcones reduced the expression of cell death-related proteins such as Bcl-2 and cyclin D1 and promoted the cleavage of PARP. However, only trans-chalcone induced the expression of the CIDEA gene and protein in these two experiments. Furthermore, transient transfections of MCF-7 using a construction of a promoter-luciferase vector showed that trans-chalcone induced the expression of the CIDEA promoter activity in 24 and 48 h. In conclusion, the results showed that trans-chalcone promoted high induction of the CIDEA promoter gene and protein, which is related to DNA fragmentation during apoptosis. (C) 2016 Published by Elsevier Masson SAS.

关键词： Apoptosis CIDEA Comet assay Licochalcone A MCF-7 Trans-chalcone pcr array

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：