检索结果-内蒙古大学图书馆

Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XXI

作者： Doblas, Ana King, Sharon V. Patwary, Nurmohammed Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Valencia Dept Opt E-46100 Burjassot Spain Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819498625

point-spread function engineering (PSF), achieved by placing a phase mask at the pupil plane of the imaging lens to encode the wavefront emerging from an imaging system, can be employed to reduce the impact of spherical aberration (SA) in 3D microscopy. In a previous study, the effect of SA on a confocal scanning microscope using a squared cubic phase mask (SQUBIC) was investigated using computer simulations. Here the effect of the SQUBIC design parameter on the insensitivity of the engineered PSF to SA is investigated using a metric based on the second-order moment of the axial variability of the PSF. We show that it is possible to find the optimum SQUBIC for the insensitization to SA.

关键词： point-spread function engineering wavefront coding spherical aberration

来源：评论

学校读者我要写书评

暂无评论

Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

Performance evaluation of point-spread function engineering ...

引用

Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIX

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In this study, we evaluated a point-spread function (PSF) engineered using wave front encoding (WFE) and a generalized cubic phase mask (GCPM) design selected to reduce the impact of depth-induced spherical aberration (SA) on extended depth-of-field (EDOF) microscopy with high NA lenses. Mean-square-error based metrics computed from three-dimensional (3D) depth-variant WFE-PSFs with increasing amounts of SA were used to quantify the engineered PSF's sensitivity to SA and to compare it to the sensitivity of the cubic-phase mask (CPM) PSF traditionally used for EDOF microscopy. The potential performance of the engineered PSF with resilience to SA was further evaluated with simulations in which EDOF images of a 3D object were obtained by processing WFE images with and without SA. A qualitative and quantitative comparison of the EDOF images with the true object show that the WFE-PSF engineering with the selected GCPM design provides better performance in reducing the impact of SA. In addition, the GCPM-based EDOF images do not suffer from the known lateral shift of object features located away from the plane of focus encountered in traditional CPM-based EDOF images.

关键词： Extended depth-of-field microscopy point-spread function engineering wavefront encoding spherical aberration

来源：评论

学校读者我要写书评

暂无评论

Recent advances in point spread function engineering and related computational microscopy approaches: from one viewpoint

引用

Biophysical Reviews 2020年第6期12卷 1303-1309页

作者： Shechtman, Yoav Department of Biomedical Engineering and Lorry Lokey Interdisciplinary Center for Life Sciences and Engineering Technion–Israel Institute of Technology Haifa 3200003 Israel

This personal hybrid review piece, written in light of my recipience of the UIPAB 2020 young investigator award, contains a mixture of my scientific biography and work so far. This paper is not intended to be a comprehensive review, but only to highlight my contributions to computation-related aspects of super-resolution microscopy, as well as their origins and future directions. © 2020, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature.

关键词： Deep learning point-spread function engineering Single particle tracking Super resolution microscopy

来源：评论

学校读者我要写书评

暂无评论

Three-Dimensional Sub-100 nm Super-Resolution Imaging of Biological Samples Using a Phase Ramp in the Objective Pupil

引用

Nano Research 2011年第6期4卷 589-598页

作者： David Baddeley Mark B. Cannell Christian Soeller Department of Physiology University of Auckland Private Bag 92019 Auckland 1142 New Zealand

Localisation microscopy overcomes the diffraction limit by measuring the position of individual molecules to obtain optical images with a lateral resolution better than 30 nm. Single molecule localisation microscopy was originally demonstrated only in two dimensions but has recently been extended to three dimensions. Here we develop a new approach to three-dimensional （3D） localisation microscopy by engineering of the point-spread function （PSF） of a fluorescence microscope. By introducing a linear phase gradient between the two halves of the objective pupil plane the PSF is split into two lateral lobes whose relative position depends on defocus. Calculations suggested that the phase gradient resulting from the very small tolerances in parallelism of conventional slides made from float glass would be sufficient to generate a two-lobed PSF. We demonstrate that insertion of a suitably chosen microscope slide that occupies half the objective aperture combined with a novel fast fitting algorithm for 3D localisation estimation allows nanoscopic imaging with detail resolution well below 100 nm in all three dimensions （standard deviations of 20, 16, and 42 nm in x, y, and z directions, respectively）. The utility of the approach is shown by imaging the complex 3D distribution of microtubules in cardiac muscle cells that were stained with conventional near infrared fluorochromes. The straightforward optical setup, minimal hardware requirements and large axial localisation range make this approach suitable for many nanoscopic imaging applications.

关键词： Single molecules nanoscopy point-spread function engineering immunocytochemistry localisation microscopy

来源：评论

学校读者我要写书评

暂无评论

Fast and Artifact-Free Excitation Multiplexing Using Synchronized Image Scanning

引用

NANO LETTERS 2024年第36期24卷 11141-11148页

作者： Bruggeman, Ezra van den Eynde, Robin Amouroux, Baptiste Venneman, Tom Vanden Berghe, Pieter Muller, Marcel Vandenberg, Wim Dedecker, Peter Katholieke Univ Leuven Dept Chem B-3001 Leuven Belgium Univ Cambridge Yusuf Hamied Dept Chem Cambridge CB2 1EW England Katholieke Univ Leuven Dept Chron Dis Metab & Ageing B-3001 Leuven Belgium

Multicolor fluorescence microscopy is an essential tool to visualize structures and dynamics in the life and materials sciences. However, the near-simultaneous acquisition of labels differing in excitation spectrum is difficult and renders such measurements prone to artifacts. We present a simple strategy to provide quasi-simultaneous fluorescence imaging with multiple excitation wavelengths by using an optical element to displace the sample image on the sensor at a rate that is much faster than the image acquisition rate and synchronizing this with the illumination. The emission elicited by the different wavelengths can then be encoded into the point-spread function of the imaging or visualized as multiple distinct images. In doing so, our approach can eliminate or mitigate artifacts caused by temporal aliasing in conventional sequential imaging. We demonstrate the use of our system to uncover hidden emissive states in single quantum dots and for the imaging of Ca2+ signaling in neurons.

关键词： fluorescence microscopy multicolor imaging point-spread function engineering single-molecule spectroscopy biosensor imaging

来源：评论

学校读者我要写书评

暂无评论

Double helix PSF engineering for computational fluorescence microscopy imaging

Double helix PSF engineering for computational fluorescence ...

引用

Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIX

作者： Ghosh, Sreya Quirin, Sean A. Grover, Ginni Piestun, Rafael Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Colorado Dept Elect Comp & Energy Eng Boulder CO 80309 USA

ISBN: (纸本)9780819488701

point spread function engineering with a double helix (DH) phase mask has been recently used in a joint computational-optical approach for the determination of depth and intensity information from fluorescence images. In this study, theoretically determined DH-PSFs computed from a model that incorporates different amounts of depth-induced spherical aberration (SA) due to refractive-index mismatch in the three-dimensional imaging layers, are evaluated through a comparison to empirically determined DH-PSFs measured from quantum dots. The theoretically-determined DH-PSFs show a trend that captures the main effects observed in the empirically-determined DH-PSFs. Calibration curves computed from these DH-PSFs show that SA slows down the rate of rotation observed in a DH-PSF which results in: 1) an extended range of rotation;and 2) asymmetric rotation ranges as the focus is moved in opposite directions. Thus, for accurate particle localization different calibration curves need to be known for different amounts of SA. Results also show that the DH-PSF is less sensitive to SA than the conventional PSF. Based on this result, it is expected that fewer depth-variant (DV) DH-PSFs will be required for 3D computational microscopy imaging in the presence of SA compared to the required number of conventional DV PSFs.

关键词： point-spread function engineering Double-Helix point-spread function Spherical Aberration Computational Imaging 3D Fluorescence microscopy

来源：评论

学校读者我要写书评

暂无评论

Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask 25

Tradeoff between insensitivity to depth-induced spherical ab...

引用

Conference on Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV

作者： Doblas, Ana Dutta, Ananya Saavedra, Genaro Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Previously, a wavefront encoded (WFE) imaging system implemented using a squared cubic (SQUBIC) phase mask has been verified to reduce the sensitivity of the imaging system to spherical aberration (SA). The strength of the SQUBIC phase mask and, as consequence, the performance of the WFE system are controlled by a design parameter, A. Although the higher the A-value, the more tolerant the WFE system is to SA, this is accomplished at the expense of the effective imaging resolution. In this contribution, we investigate this tradeoff in order to find an optimal A-value to balance the effect of SA and loss of resolution.

关键词： three-dimensional microscopy wavefront encoding point-spread function engineering resolution limit

来源：评论

学校读者我要写书评

暂无评论

Reducing depth induced spherical aberration in 3D widefield fluorescence microscopy by wavefront coding using the SQUBIC phase mask

Reducing depth induced spherical aberration in 3D widefield ...

引用

Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XXI

作者： Patwary, Nurmohammed Doblas, Ana King, Sharon V. Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (纸本)9780819498625

Imaging thick biological samples introduces spherical aberration (SA) due to refractive index (RI) mismatch between specimen and imaging lens immersion medium. SA increases with the increase of either depth or RI mismatch. Therefore, it is difficult to find a static compensator for SA(1). Different wavefront coding methods(2,3) have been studied to find an optimal way of static wavefront correction to reduce depth-induced SA. Inspired by a recent design of a radially symmetric squared cubic (SQUBIC) phase mask that was tested for scanning confocal microscopy(1) we have modified the pupil using the SQUBIC mask to engineer the point spread function (PSF) of a wide field fluorescence microscope. In this study, simulated images of a thick test object were generated using a wavefront encoded engineered PSF (WFE-PSF) and were restored using space-invariant (SI) and depth-variant (DV) expectation maximization (EM) algorithms implemented in the COSMOS software(4). Quantitative comparisons between restorations obtained with both the conventional and WFE PSFs are presented. Simulations show that, in the presence of SA, the use of the SIEM algorithm and a single SQUBIC encoded WFE-PSF can yield adequate image restoration. In addition, in the presence of a large amount of SA, it is possible to get adequate results using the DVEM with fewer DV-PSFs than would typically be required for processing images acquired with a clear circular aperture (CCA) PSF. This result implies that modification of a widefield system with the SQUBIC mask renders the system less sensitive to depth-induced SA and suitable for imaging samples at larger optical depths.

关键词： Three-dimensional microscopy wavefront encoding point-spread function engineering image restoration phase mask

来源：评论

学校读者我要写书评

暂无评论

3D Microscope Imaging Robust to Restoration Artifacts Introduced by Optically Thick Specimens 22

3D Microscope Imaging Robust to Restoration Artifacts Introd...

引用

Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XXII

作者： Patwary, Nurmohammed King, Sharon V. Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9781628414202

We demonstrate 3D microscope imaging using computational optical sectioning microscopy (COSM) with an engineered point-spread function (PSF) robust to depth-induced spherical aberration (SA). Earlier we demonstrated that wavefront encoding (WFE) using a squared cubic (SQUBIC) phase mask reduces the PSF depth-variance in the presence of SA and that space-invariant (SI) restoration of simulated images using a single WFE-PSF does not lead to artifacts as in the conventional case. In this study, we show experimental verification of our WFE COSM approach. The WFE system used is a commercial microscope with a modified side port imaging path, where a spatial light modulator projects the SQUBIC phase mask on the back focal plane of the imaging lens. High resolution images of a test sample with 6 mu m in diameter microspheres embedded in UV-cured optical cement (RI = 1.47) were captured using both the engineered and the conventional imaging paths of the system. The acquired images were restored using a regularized SI expectation maximization algorithm based on Tikhonov-Miller regularization with a roughness penalty. A comparative study quantified in terms of the correlation coefficients between the XZ medial sections of the restored images, from experimental data, shows an 11% reduction in depth sensitivity in the SQUBIC system compared to the conventional system.

关键词： Three-dimensional microscopy wavefront encoding point-spread function engineering three-dimensional image restoration spherical aberration

来源：评论

学校读者我要写书评

暂无评论

Dielectric metasurfaces for distance measurements and three-dimensional imaging

引用

Advanced Photonics 2019年第3期1卷 20-28页

作者： Chunqi Jin Mina Afsharnia RenéBerlich Stefan Fasold Chengjun Zou Dennis Arslan Isabelle Staude Thomas Pertsch Frank Setzpfandt Friedrich Schiller University Jena Institute of Applied PhysicsAbbe Center of PhotonicsJenaGermany Chinese Academy of Sciences Changchun Institute of OpticsFine Mechanics and PhysicsChangchunChina University of the Chinese Academy of Sciences BeijingChina Fraunhofer Institute for Applied Optics and Precision Engineering JenaGermany

Ultrathin metasurfaces have shown the capability to influence all aspects of light *** has made them promising options for replacing conventional bulky imaging optics while adding advantageous optical properties or *** demonstrate that such metasurfaces can also be applied for single-lens three-dimensional(3-D)imaging based on a specifically engineered point-spread function(PSF).Using Huygens’metasurfaces with high transmission,we design and realize a phase mask that implements a rotating PSF for 3-D *** experimentally characterize the properties of the realized double-helix PSF,finding that it can uniquely encode object distances within a wide ***,we experimentally demonstrate wide-field depth retrieval within a 3-D scene,showing the suitability of metasurfaces to realize optics for 3-D imaging,using just a single camera and lens system.

关键词： optics nanophotonics metasurfaces Mie-resonances three-dimensional imaging point-spread function engineering

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：