作者：
Zhao, MengyaoZhuang, HongjunZhang, HongxinLi, BenhaoMing, JiangChen, XiaoyuanChen, MeiwanUniv Macau
Inst Chinese Med Sci State Key Lab Qual Res Chinese Med Macau 999078 Peoples R China Natl Univ Singapore
Dept Diagnost Radiol Yong Loo Lin Sch Med Singapore 119074 Singapore Natl Univ Singapore
Dept Surg Yong Loo Lin Sch Med Singapore 119074 Singapore Natl Univ Singapore
Dept Chem & Biomol Engn Yong Loo Lin Sch Med Singapore 119074 Singapore Natl Univ Singapore
Dept Biomed Engn Yong Loo Lin Sch Med Singapore 119074 Singapore Natl Univ Singapore
Fac Engn Singapore 119074 Singapore Natl Univ Singapore
Yong Loo Lin Sch Med Clin Imaging Res Ctr Ctr Translat Med Singapore 117599 Singapore Natl Univ Singapore
Yong Loo Lin Sch Med Nanomed Translat Res Program NUS Ctr Nanomed Singapore 117597 Singapore ASTAR
Inst Mol & Cell Biol Singapore 138673 Singapore Fudan Univ
Shanghai Key Lab Mol Catalysis & Innovat Mat State Key Lab Mol Engn Polymers & iChem Dept Chem Shanghai 200433 Peoples R China
Designing luminescence lifetime sensors in the second near-infrared (NIR-II) window is a great challenge due to the difficult structural construction. Here, we report a tumor redox responsive and easily synthesized ma...
详细信息
Designing luminescence lifetime sensors in the second near-infrared (NIR-II) window is a great challenge due to the difficult structural construction. Here, we report a tumor redox responsive and easily synthesized material, amorphous manganese oxide (MnOx) with indirect band gap of 1.02 eV, as an energy acceptor to build a luminescence resonance energy transfer (LRET) toolbox for universally regulating NIR-I to NIR-II luminescence lifetimes of lanthanide nanoparticles, in which energy transfer is based on matched energy gap instead of conventional overlapped spectra. We further utilize ytterbium (Yb3+)-doped YbNP@MnOx as an NIR-II luminescence lifetime sensor to realize in vitro quantitative redox visualization with relative errors under 5 % in samples covered with mouse skin. Furthermore, HepG2 cells and tumors with high redox state have been accurately distinguished by NIR-II luminescence lifetime imaging. The quantified intracellular and intratumor glutathione (GSH) levels are highly consistent with the commercial kit results, illustrating the reliable redoxvisualization ability in biological tissue.
Designing luminescence lifetime sensors in the second near-infrared (NIR-II) window is a great challenge due to the difficult structural construction. Here, we report a tumor redox responsive and easily synthesized ma...
详细信息
Designing luminescence lifetime sensors in the second near-infrared (NIR-II) window is a great challenge due to the difficult structural construction. Here, we report a tumor redox responsive and easily synthesized material, amorphous manganese oxide (MnO x ) with indirect band gap of 1.02 eV, as an energy acceptor to build a luminescence resonance energy transfer (LRET) toolbox for universally regulating NIR-I to NIR-II luminescence lifetimes of lanthanide nanoparticles, in which energy transfer is based on matched energy gap instead of conventional overlapped spectra. We further utilize ytterbium (Yb 3+ )-doped YbNP@MnO x as an NIR-II luminescence lifetime sensor to realize in vitro quantitative redox visualization with relative errors under 5 % in samples covered with mouse skin. Furthermore, HepG2 cells and tumors with high redox state have been accurately distinguished by NIR-II luminescence lifetime imaging. The quantified intracellular and intratumor glutathione (GSH) levels are highly consistent with the commercial kit results, illustrating the reliable redoxvisualization ability in biological tissue.
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