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Molecular diversity of rna-2 genome segments in pecluviruses causing peanut clump disease in West Africa and India

ARCHIVES OF VIROLOGY 2003年第1期148卷 83-98页

作者： Naidu, RA Sawyer, S Deom, CM Univ Georgia Dept Plant Pathol Athens GA 30602 USA Int Crops Res Inst Semi Arid Trop Patancheru Andhra Pradesh India Washington Univ Dept Math St Louis MO 63130 USA Washington Univ Dept Genet St Louis MO 63130 USA

The complete nucleotide sequence of rna-2 genome segments of four isolates of Peanut clump virus (PCV) and two isolates of Indian peanut clump virus (IPCV) were determined. Comparisons among the complete rna-2 sequences of six isolates from this study and two published earlier, revealed a high degree of variability in size (between 4290 and 4652 nucleotides) and nucleotide sequence identities (between 58% and 79%). Amino acid sequence alignments of the five open reading frames (ORF) showed that ORF 4, which encodes the second of the triple gene block proteins, is highly conserved (90 to 98% identical) whereas the protein encoded by ORF 2, whose function is unknown, is less conserved (25 to 60% identical). The coat protein of the eight isolates showed amino acid identities between 37% and 89% and contained several conserved residues. Phylogenetic comparisons, based on complete rna-2 sequences, revealed that the eight isolates grouped into two distinct clusters with no geographical distinction between PCV and IPCV isolates. Phylogenetic tree topologies for individual ORFs showed an overall similarity with that obtained from entire rna-2 sequences, although the relative positions of individual isolates vary within each cluster. The results indicate that there is substantial divergence among the rna-2 genomes of pecluviruses and suggest that different proteins have evolved differently, possibly due to different selection pressures.

关键词：非洲西部氨基酸序列花生/病毒学碱基序列病毒壳体/化学衣壳蛋白质类/化学保守序列基因病毒印度分子序列数据开放读码框架种系发生植物病毒/化学植物病毒/分类植物病毒/遗传学植物病毒/分离和提纯 rna病毒/化学 rna病毒/分类 rna病毒/遗传学 rna病毒/分离和提纯 rna 病毒/遗传学 rna 病毒/分离和提纯序列比对

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The complete cDNA sequence of a type II Trichomonas vaginalis virus

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VIROLOGY 2000年第2期267卷 350-359页

作者： Bessarab, IN Liu, HW Ip, CF Tai, JH Acad Sinica Inst Biomed Sci Div Infect Dis Taipei Taiwan

Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T: vaginalis, are a group of divergent double-stranded (ds) rna viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds rna from a newly identified TVV2-1 isolate was determined. The sequence of the plus-strand mrna contains four open reading frames, which encode overlapping cap and pol genes in the reading frame 2 and reading frame 1, respectively, and two putative serine-threonine-rich basic proteins VP3 and VP4 in the third reading frame. An 85-kDa capsid protein and a 180-kDa CAP-POL fusion protein were identified in crude viruses by Western blotting experiments using antisera raised against gene-specific oligopeptides. In conjunction with the presence of a potential ribosomal slippery heptanucleotide G GGC CCC within the overlap of the cap and pol genes, these observations suggest that the pol gene of TVV2-1 is translated via a -1 ribosomal frameshifting event during translation of the cap gene. Our results also provide insight into the conservation among divergent dsrna species from TW and suggest that the genome of TVV2-1 may encode two extra genes in addition to the cap and pol genes. (C) 2000 Academic Press.

关键词：氨基酸序列病毒壳体/遗传学克隆分子 DNA 互补/化学 DNA 互补/遗传学指导DNA的rna聚合酶类/遗传学基因组病毒模型分子分子序列数据开放读码框架 rna病毒/遗传学 rna病毒/分离和提纯 rna病毒/超微结构 rna 病毒/化学 rna 病毒/遗传学序列比对序列分析 DNA 毛滴虫阴道/病毒学动物

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Comparison of nucleotide sequences between northern and southern Philippine isolates of rice grassy stunt virus indicates occurrence of natural genetic reassortment

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VIROLOGY 2000年第1期266卷 26-32页

作者： Miranda, GJ Azzam, O Shirako, Y Univ Tokyo ANESC Asian Ctr Bioresources & Environm Sci Bunkyo Ku Tokyo 1138657 Japan Univ Tokyo Grad Sch Agr Life Sci Bunkyo Ku Tokyo 1138657 Japan Int Rice Res Inst Makati 1271 Philippines

Rice grassy stunt virus is a member of the genus Tenuivirus, is persistently transmitted by a brown planthopper, and has occurred in rice plants in South, Southeast, and East Asia (similar to North and South America). We determined the complete nucleotide (nt) sequences of rnas 1 (9760 nt), 2 (4069 nt), 3 (3127 nt), 4 (2909 nt), 5 (2704 nt), and 6 (2590 nt) of a southern Philippine isolate from South Cotabato and compared them with those of a northern Philippine isolate from Laguna (Toriyama et al., 1997 1998). The numbers of nucleotides in the terminal untranslated regions and open reading frames were identical between the two isolates except for the 5' untranslated region of the complementary strand of rna 4. Overall nucleotide differences between the two isolates were only 0.08% in rna 1, 0.58% in rna 4, and 0.26% in rna 5, whereas they were 2.19% in rna 2, 8.38% in rna 3, and 3.63% in rna 6. In the intergenic regions, the two isolates differed by 9.12% in rna 2, 11.6% in rna 3, and 6.86% in rna 6 with multiple consecutive nucleotide deletion/insertions, whereas they differed by only 0.78% in rna 4 and 0.34% in rna 5. The nucleotide variation in the intergenic region of rna 6 within the South Cotabato isolate was only 0.33%. These differences in accumulation of mutations among individual rna segments indicate that there was genetic reassortment in the two geographical isolates;rnas 1, 4, and 5 of the two isolates came from a common ancestor, whereas rnas 2, 3, and 6 were from two different ancestors. (C) 2000 Academic Press.

关键词：氨基酸序列 DNA 互补/遗传学分子序列数据突变水稻/病毒学菲律宾植物病毒/遗传学植物病毒/分离和提纯 rna病毒/遗传学 rna病毒/分离和提纯 rna 病毒/遗传学 rna 病毒/分离和提纯再装配病毒/遗传学再装配病毒/分离和提纯序列比对序列分析 DNA 变异(遗传学) 病毒蛋白质类/化学病毒蛋白质类/遗传学

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SYNOVIAL MEMBRANE AND FLUID MORPHOLOGIC ALTERATIONS IN EARLY RHEUMATOID-ARTHRITIS - MICROVASCULAR INJURY AND VIRUS-LIKE PARTICLES

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ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1975年第JUN13期256卷 39-64页

作者： SCHUMACHER, HR UNIV PENN SCH MED DEPT MED PHILADELPHIA PA 19104 USA VET ADM HOSP ARTHRITIS SECT PHILADELPHIA PA 19104 USA

Eight patients have been studied during the first 6 weeks of rheumatoid synovitis. All of them exhibited microvascular injury, which was manifested by gaps between endothelial cells, vascular occlusion, erythrocyte extravasation, or endothelial cell injury. In four patients, a variety of virus-like particles were found associated with the endothelium or perivascular cells. In two cases, particles were seen in electron-dense deposits in vessel walls. Lymphocytes and PMN infiltrated the synovial membranes, but plasma cells were uncommon. Evidence of phagocytosis was prominent in synovial lining cells and other large mononuclear cells, but not in PMN. These observations are consistent with injury to synovium and, specifically, synovial vessels as an early stage in RA synovitis. The virus-like particles require further investigation, because nonviral cell components remain very difficult to distinguish in electron microscopy tissue sections.

关键词：关节炎类风湿/微生物学关节炎类风湿/病理学基膜/超微结构活组织检查针吸毛细血管/超微结构内皮/微生物学内皮/超微结构淋巴细胞/病理学中性白细胞/病理学吞噬作用浆细胞/病理学 rna病毒/分离和提纯滑液/细胞学滑膜/血液供给滑膜/微生物学滑膜/病理学成年人女(雌)性人类男(雄)性中年人

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