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A novel application of ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry for the rapid quantification of acrylamide in coffee

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JOURNAL OF FOOD COMPOSITION AND ANALYSIS 2025年 141卷

作者： Wang, Peng Zhang, Nianhua Hu, Zhengyan Wu, Pinggu Mao, Jiawen Zhang, Jingshun Cai, Zengxuan Wang, Zhiyuan Wang, Junlin Zhejiang Prov Ctr Dis Control & Prevent Hangzhou 310051 Peoples R China Hangzhou Med Coll Hangzhou 311399 Peoples R China

We developed a simple, rapid, accurate, and sensitive method for the determination of acrylamide (AA) in coffee based on ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/ MS). The AA in coffee was simply extracted with water and then defatted with dichloromethane. After that, concentration and purification were achieved through solid-phase extraction techniques. The calibration curve for AA showed excellent linearity over the concentration range of 0.5-500 ng/mL, with a correlation coefficient (r) in excess of 0.999. Furthermore, our method exhibited high precision, as evidenced by intra- and inter-day coefficients of variation of less than 5.7 % and 6.3 %, respectively. The analytical accuracy for AA quantification ranged from 98.0 % to 105.2 %, and the limit of detection and limit of quantification for AA in coffee were determined to be 1.5 mu g/kg and 5.0 mu g/kg, respectively. In addition, the applicability of the proposed method was assessed through its implementation in the quantification of AA in both roasted and instant coffee. The results prove that the method is simple, rapid, accurate, and sensitive, making it as an excellent option for the detection of AA in coffee.

关键词： Acrylamide Coffee Solid phase extraction UPLC-MS/MS rapid quantification

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rapid quantification of trace chloramphenicol in honey under ambient conditions using direct analysis via real-time QTRAP mass spectrometry

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FOOD CHEMISTRY 2019年 276卷 50-56页

作者： Li, Xiu Qin Li, Hong Mei Xu, Sen Gao, Yan Zhang, Qing He Zhang, Yan Feng, Meng Yu Natl Inst Metrol Div Chem Metrol & Analyt Sci Beijing 100029 Peoples R China Cheng Du Inst Food & Drug Control Chengdu 610041 Sichuan Peoples R China

In this paper, we employed a direct analysis in real time (DART) ion source coupled to QTRAP mass spectrometry for the analysis of chloramphenicol (CAP) in honey. The accuracy of the DART-MS/MS method for the analysis of CAP in honey was evaluated by comparison with data generated by a validated HPLC-MS/MS method. The sample preparation procedure was optimized to obtain sensitive and accurate determination of trace CAP residue in honey at concentrations less than 1.0 mu g/kg. The DART-MS/MS method offers faster analysis time, lower cost per analysis, and reduced matrix effects and simplicity compared to HPLC-MS/MS method. Fifty-two honey samples collected from a Chinese market were analyzed using two methods. The results of the two methods are in good agreement, suggesting DART-MS/MS as a potential technique for the direct detection of trace amounts of veterinary drugs in complex matrixes.

关键词： Chloramphenicol (CAP) Honey Direct analysis in real time (DART) rapid quantification QTRAP mass spectrometry

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rapid quantification of human urinary estrogens and estrogen metabolites by HPLC mass spectrometry

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MICROCHEMICAL JOURNAL 2019年 147卷 157-162页

作者： You, Jia Shi, Ying Li, Jiaqi Yang, Xmoqin Liu, Zeyu Zhu, Wenli Wu, Zhigang Xiong, Jingyuan Sichuan Univ West China Sch Publ Hlth Chengdu 610041 Sichuan Peoples R China Sichuan Univ Hlth Food Evaluat Res Ctr Chengdu 610041 Sichuan Peoples R China Sichuan Univ West China Hosp Chengdu 610041 Sichuan Peoples R China Chengdu Womens & Childrens Cent Hosp Chengdu Sichuan Peoples R China Chengdu MediMass Technol CO LTD Chengdu Sichuan Peoples R China

Estrogens and estrogen metabolites have different biological properties and the individual patterns of estrogen metabolism may influence the risk of breast cancer. A rapid, sensitive and accurate HPLC-MS method for simultaneously measuring estrogens and metabolites in urine samples has been developed and validated. The method was validated and applied for analysis of breast cancer patients. Compared with previous methods, this method greatly reduces analysis time and allows for simultaneous measurement of human urinary estrogens and metabolites within 10 min for the first time. It is perfectly suitable for commercial diagnostic laboratory use due to its sensitivity, specificity and high throughput.

关键词： Estrogen metabolites Urinary estrogen HPLC-MS Breast cancer rapid quantification

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rapid quantification of a microbial surfactant by a simple turbidometric method

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JOURNAL OF MICROBIOLOGICAL METHODS 2009年第1期76卷 38-42页

作者： Mukherjee, Soumen Das, Palashpriya Sen, Ramkrishna Indian Inst Technol Dept Biotechnol Kharagpur 721302 W Bengal India

quantification of the biosurfactants produced by a variety of microorganisms is a time taking and difficult task due to the lack of rapid, efficient and accurate methods. This work presents a simple turbidometric method for quantification of crude biosurfactants based on their property to become insoluble at low pH values. Biosurfactants obtained from a Bacillus sp. using different carbon substrates showed a good linear correlation (R-2>0.99) between biosurfactant concentrations and turbidity in the range of 1 to 10 g L-1 of crude biosurfactants. The substrate specific equations (SSE) and generalized equations (GE) developed in this work effectively predicted the amount of crude biosurfactant produced indifferent sets of fermentation experiments validating the method. A similar linear correlation was also observed with biosurfactants obtained from two other strains, Bacillus circulans and Pseudomonas sp. This simple method may prove to be effective in fast, accurate and inexpensive quantification of crude biosurfactants produced by diverse bacteria. (C) 2008 Elsevier B.V. All rights reserved.

关键词： Turbidometry rapid quantification Microbial surfactant Generalized calibration

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rapid quantification of Left Ventricular Function and Mass Using Transoesophageal Three-dimensional Echocardiography: Validation of a Method that uses Long-axis Cutplanes*

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European Journal of Echocardiography 2000年第3期1卷 213-221页

作者： Kühl, H.P. Franke, A. Merx, M. Hoffmann, R. Puschmann, D. Hanrath, P. Medical Clinic I University Hospital Aachen Germany

Aims: Despite its proven superiority compared to conventional echocardiographic techniques, three-dimensional (3D) echocardiography has not gained widespread acceptance in clinical medicine for the quantification of left ventricular volumes, function and mass. This is mainly due to the large, time-consuming process of data analysis. We sought to validate a new method that enables the accurate quantification of the left ventricle in a clinically acceptable short period of time. Methods and Results: Left ventricular volumes, ejection fraction and mass were determined in 44 patients using 3D echocardiography. The 3D echocardiographic data sets were analysed: (i) using the conventional 'summation of slices' algorithm (slice thickness 5 and 10mm), which is based on the analysis of the 3D reconstructed left ventricle in short-axis cross-sections;and (ii) using the new method which is based on the analysis of the 3D reconstructed left ventricle in long-axis images. In each patient measurements were repeated using 3, 6, 7, 8, 9, 12 and 15 long-axis images. For all volumetric measurements there was a continuous reduction of measurement variability using increasing numbers of long-axis images. The use of more than nine long-axis images for volumes, and eight long-axis images for ejection fraction and mass, did not result in a further reduction of variability. The analysis time for volumes and masses averaged less than 5min for the long-axis method using nine component images, compared to 20-43min for the short-axis method. Conclusion: 3D echocardiography combined with a novel method based on the analysis of long-axis cross-section allows accurate quantification of left ventricular volumes, function and mass in a clinically acceptable short period of time. In the future, the combination of a real-time 3D echocardiographic acquisition technique with this analysis method should have important implications for the introduction of 3D echocardiography in clinical practice. © 2000 T

关键词： Three-dimensional echocardiography left ventricular volume ejection fraction left ventricular mass long-axis analysis method rapid quantification

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Microfluidic rapid quantification of Salmonella enterica serovar Typhimurium collected from chicken meat using immunomagnetic separation after formaldehyde treatment

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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY 2021年第10期56卷 5402-5408页

作者： Tokunaga, Yusuke Wakabayashi, Yuki Yonogi, Shinya Saito, Mamoru Yamaguchi, Nobuyasu Osaka Inst Publ Hlth Higashinari Ku 1-3-69 Nakamichi Osaka 5370025 Japan Osaka Res Inst Ind Sci & Technol Joto Ku 1-6-50 Morinomiya Osaka 5368553 Japan

Foodborne pathogens such as Salmonella enterica serovar Typhimurium, which cause self-limiting gastroenteritis, are important in food safety and public health. Detection of Salmonella in complex food matrices is required for rapid and effective monitoring of contaminated food products. In this study, we collected Salmonella Typhimurium in chicken using immunomagnetic separation after formaldehyde treatment. Collected Salmonella Typhimurium cells were then quantified microfluidic device or fluorescence microscopy. The recovery ratio obtained with immunomagnetic separation after formaldehyde treatment was 77 (+/- 5)% for the microscope count and 63 (+/- 9)% for the microfluidic count. According to the fluorescence microscope images, the food matrix was effectively removed from the collected cell suspension. The counts of Salmonella Typhimurium cells obtained by the microfluidic device and fluorescence microscope were closely correlated (R-2 = 0.993) with a range of 3.3 (+/- 0.5) x 10(3) to 1.2 (+/- 0.5) x 10(6) cells g(-1). Our method provides quantitative detection of Salmonella Typhimurium within 3-4 h and could be used to efficiently evaluate microbial risk of quality control of foodstuffs and prevention of foodborne disease.

关键词： fluorescent antibody foodborne pathogen immunomagnetic separation microfluidic device rapid quantification Salmonella Typhimurium

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Soluble Expression and rapid quantification of GFP-hepA Fusion Protein in Recombinant Escherichia coli

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Chinese Journal of Chemical Engineering 2007年第1期15卷 122-126页

作者：陈银邢新会叶逢春况莹 Department of Chemical Engineering Tsinghua University Beijing 100084 China Department of Biological Sciences the University of Warwick Coventry CV4 7AL UK Department of Chemical Engineering Tsinghua University Beijing 100084 China

To establish a rapid quantification method for heparinase I during its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase I to the C terminus of a green fluorescent protein mutant （GFPmutl）. As a result, not only was the functional recombinant expression of heparinase I in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase I activity, allowing enzyme activity to be quantified rapidly during the fermentation.

关键词： functional expression fusion protein green fluorescent protein (GFP) heparinase I rapid quantification

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Autonomous system for rapid field quantification of Escherichia coli in surface waters

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JOURNAL OF APPLIED MICROBIOLOGY 2019年第1期126卷 332-343页

作者： Angelescu, D. E. Huynh, V. Hausot, A. Yalkin, G. Plet, V. Mouchel, J-M. Guerin-Rechdaoui, S. Azimi, S. Rocher, V. Fluidion SAS R&D Div 231 Rue St Honore F-75001 Paris France Sorbonne Univ CNRS EPHE UMR Metis 7619 Paris France SIAAP Direct Innovat Environm Colombes France

Aims The purpose of this work is to present and evaluate the performance of a novel Automatic Lab-in-vial Escherichia coli Remote Tracking technology based on an automated real-time defined substrate approach, implemented in both portable and in situ instruments. Methods and Results We present the fresh water calibration procedure, and assess performance using side-by-side comparison with most probable number (MPN) approaches in terms of accuracy, reproducibility and capability to correctly generate early-warning alerts. Long-term data from an operational in situ deployment at a potential bathing site is presented as well. Conclusions Automatic Lab-in-vial Escherichia coli Remote Tracking technology is shown to be an accurate and rapid bacterial quantification technology, capable of autonomous in situ measurements with metrological capabilities comparable to those of an approved laboratory using MPN microplate techniques. Significance and Impact of the Study rapid quantification of bacterial pollution is a requirement in water quality applications ranging from recreational water use, agriculture and aquaculture to drinking and wastewater treatment. The method and instruments presented in this work should enable fast and accurate bacterial concentration measurements to be performed in a portable or in situ manner, thus simplifying operational logistics, reducing time-to-result delays, and eliminating sample transportation constraints associated with traditional techniques.

关键词： Escherichia coli (all potentially pathogenic types) environmental/recreational water rapid quantification rapid test surface waters wastewater water quality

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rapid and environmentally friendly quantification of myricetin and dihydromyricetin in vine tea using direct infusion-tandem mass spectrometry

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JOURNAL OF FOOD COMPOSITION AND ANALYSIS 2025年 140卷

作者： Wang, Wenhao Tan, Guoying Lei, Qinggui Chen, Jing Qian, Zhengming Jinan Univ Coll Pharm Inst Tradit Chinese Med & Nat Prod Guangzhou 510632 Guangdong Peoples R China Dongguan HEC Cordyceps R&D Co Ltd Key Lab State Adm Tradit Chinese Med Dongguan 523871 Guangdong Peoples R China Xiangnan Univ Coll Med Imaging Lab & Rehabil Dept Rehabil Med Chenzhou 423000 Hunan Peoples R China

A rapid and environmentally friendly quantification method for myricetin and dihydromyricetin in vine tea was developed using direct infusion-tandem mass spectrometry (DI-MS/MS). These compounds are vital functional components known for their anti-inflammatory and antioxidant properties, making them significant for health promotion. DI-MS/MS enhances analytical efficiency by eliminating the need for chromatographic separation, significantly reducing analysis time and solvent consumption compared to previous methods like liquid chromatography-tandem mass spectrometry. This method was validated for accuracy, precision, and stability, ensuring reliable results across various samples. By providing a more sustainable and rapid method for the quality control of vine tea products, this method contributes significantly to the industrial development of vine tea as a functional food product, thereby supporting consumer safety and health benefits in the growing market for herbal teas and natural health products.

关键词： Vine tea Myricetin Dihydromyricetin Direct infusion-tandem mass spectrometry rapid quantification Herbal tea Functional food Environmental sustainability

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rapid Real-Time PCR using photothermal conversion of gold nanoparticles for detection of Salmonella typhimurium

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MICROCHEMICAL JOURNAL 2025年 209卷

作者： Ahn, Jae Sung Jeong, Do Hyun Ahn, Sun Hee Pukyong Natl Univ Smart Gym Based Translat Res Ctr Act Sr Healthcare Busan South Korea Korea Photon Technol Inst Bio & Hlth Photon Res Ctr Gwangju South Korea

This study presents an innovative photothermal quantitative PCR (PT-qPCR) method for rapid Salmonella DNA detection, leveraging gold nanoshells for efficient photothermal conversion. The technique significantly reduces detection time to under 30 min, maintaining excellent amplification efficiency comparable to conventional PCR methods. The optimized PT-qPCR protocol achieves a detection limit of 1 picogram/mu L for Salmonella DNA, delivering consistent performance across a range of DNA concentrations. This novel approach to Salmonella detection paves the way for faster, more efficient food safety testing and public health management.

关键词： Photothermal PCR Gold Nanoparticles Salmonella Detection rapid quantification Amplification Efficiency

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