Developing analytical methods for simultaneous detection of multiple antibiotic residues is crucial for environmental protection and human health. In this study, a dual lanthanide fluorescence probe (GDP-Eu-Tb) based ...
详细信息
Developing analytical methods for simultaneous detection of multiple antibiotic residues is crucial for environmental protection and human health. In this study, a dual lanthanide fluorescence probe (GDP-Eu-Tb) based on nucleotides has been designed. The addition of quinolone antibiotics (QNs) quench the Eu3+ fluorescence signal through the inner filter effect (IFE) and exhibit characteristic peaks, enabling ratio fluorescence detection of levofloxacin (LVLX), gatifloxacin (GTLX), and moxifloxacin (MXLX). A ratiometric fluorescence sensor array is constructed using a single sensor element (GDP-Eu-Tb), combined with principalcomponent analysis (PCA) and decision tree (DT) algorithms to model the relationship between fluorescence intensity ratios (I450/I616, I460/I616, I463/I616, I468/I616) and QNs. The performance of the DT model is evaluated using accuracy, precision, recall, and F1 score, with stability and generalizability confirmed by stratified ten-fold cross-validation. This approach demonstrates high sensitivity, selectivity and applicability and provides an effective solution for antibiotic residue detection.
暂无评论