The ready-to-use,structure-supporting hydrogel bioink can shorten the time for ink preparation,ensure cell dispersion,and maintain the preset shape/microstructure without additional assistance during ***,ink with high...
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The ready-to-use,structure-supporting hydrogel bioink can shorten the time for ink preparation,ensure cell dispersion,and maintain the preset shape/microstructure without additional assistance during ***,ink with high permeability might facilitate uniform cell growth in biological constructs,which is beneficial to homogeneous tissue ***,current bioinks are hard to meet these requirements simultaneously in a simple ***,based on the fast dynamic crosslinking of aldehyde hyaluronic acid(AHA)/N-carboxymethyl chitosan(CMC)and the slow stable crosslinking of gelatin(GEL)/4-arm poly(ethylene glycol)succinimidyl glutarate(PEG-SG),we present a time-sharing structure-supporting(TSHSP)hydrogel bioink with high permeability,containing 1%AHA,0.75%CMC,1%GEL and 0.5%*** TSHSP hydrogel can facilitate printing with proper viscoelastic property and self-healing *** crosslinking with 4%PEG-SG for only 3 min,the integrity of the cell-laden construct can last for 21 days due to the stable internal and external GEL/PEG-SG networks,and cells manifested long-term viability and spreading ***-like,muscle-like,and cartilage-like in vitro constructs exhibited homogeneous cell growth and remarkable biological *** work provides not only a convenient and practical bioink for tissue engineering,targeted cell therapy,but also a new direction for hydrogel bioink development.
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