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In Silico Screening for Microsatellite Markers from Expressed Sequence Tags of Porphyra yezoensis (Bangiales, Rhodophyta)

引用

Journal of Ocean University of China 2007年第2期6卷 161-166页

作者： WANG Mengqiang HU Jingjie ZHUANG Yunyun ZHANG Lei LILT Wei MAO Yunxiang College of Marine Life Science Ocean University of China Qingdao 266003 P. R. China

The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs) were utilized to identify simple sequence repeats (SSRs), or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1 162 out of 21 954 ESTs contained microsatellites and cluster analysis indi- cated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites.

关键词： bioinformatics data mining EST microsatellites Porphyra yezoensis

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In Silico Screening for Microsateilite Markers from ESTs of Porphyra yezoensis (Bangiales,Rhodophyta)

In Silico Screening for Microsateilite Markers from ESTs of ...

引用

中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术讨论会

作者： Mengqiang Wang Jingjie Hu Wei Liu Yunxiang Mao~*(College of Marine Life Science,Ocean University of China,266003,China)

The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs)were utilized to identify simple sequence repeats(SSRs),or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high *** microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis,1 162 out of 21 954 ESTs contained microsatellites and cluster analysis indicated that 984 of these ESTs fell into 112 contigs,while the other 178 ESTs were singletons.A total of 290 unique SSR=containing genes were *** AAC SSRs were the most populous type of ***-rich microsatellites were predominant among all the microsatellites.

关键词： bioinformatics data mining EST microsatellites Porphyra yezoensis

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