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Expression of structural proteins of hepatitis c virus (HcV) in mammalian cells

Science china(Life Sciences) 1998年第1期41卷 47-55页

作者：李迎春李光地孔玉英汪垣王宇闻玉梅 1. Shanghai Institute of Biochemistry Chinese Academy of Sciences 200031 Shanghai China 2. Institute of Liver Disease Beijing Medical University 100044 Beijing China 3. Laboratory of Medical Molecular Virology Ministry of Public Health Shanghai Medical University 200031 Shanghai China

The vaccinia viral vector containing T7 promoter was used to construct the expression plasmids carrying HcV structural genes of c, E1 and E2/NS1. These genes were transiently expressed in mammalian cells in the presence of the T7 RNA polymerase which was provided by the recombinant vaccinia virus vTT7. Expression of mature core protein, envelope protein E1 and E2 was detected by Western blot using HcV patient sera as the primary antibodies. It was found that the sera from different HcV patients reacted differently with the expressed products, so did the sera collected at different times from the same patient, from whom the HcV structural genes were isolated. Among six mammalian cell lines, Vero and HeLa were the most suitable for the expression of c, E1 and E2. The recombinant vaccinia viruses have been constructed to constantly produce the c, E1 and E2 proteins for further research.

关键词： hepatitis c virus core protein envelope protein recombinant vaccinia virus transient expression immunoblotting.

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Effect of h-BN addition on thermo-physical properties and phonon mechanisms of c/c-BN composites

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cARBON 2023年 204卷 346-356页

作者： Niu, Zi-bo Xiao, Peng Zhang, Bengu Li, Zhuan chen, Fu chen, Pengju Li, Yang Cent South Univ Natl Key Lab Sci & Technol High Strength Struct Ma Changsha 410083 Peoples R China Cent South Univ Powder Met Res Inst Changsha 410083 Peoples R China

c/c-BN composites containing different contents of h-BN particles were prepared by chemical vapor infiltration (cVI), presenting different microstructures and pyrolytic carbon (Pyc) textures. The effects of different micro-structures and Pyc textures on the thermo-physical properties and their phonon mechanisms at 30-900 degrees c were investigated. The temperature dependence of heat capacity follows Debye's phonon theory, especially in the low -temperature section (30-300 degrees c) and the temperature dependence of thermal conductivity is determined by heat capacity and mean free length of phonon. Due to the higher texture of h-BN and regenerative laminar Pyc (ReL-Pyc), h-BN addition causes higher Debye temperature (Theta D) and high sensitivity of thermal conductivity to temperature. Besides, h-BN addition significantly changes the microstructure of composites such as pore struc-ture, Pyc morphology, crack distribution and fiber/Pyc interface bonding state, causing the transformation of thermal conductivity from anisotropy to isotropy. Finally, based on the different microstructures of composites, the microstructure model and thermal resistance formulas of composites were established and coincident with the experimental results.

关键词： c c composite Heat capacity Thermal conductivity Phonon mechanisms

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Effect of the incorporation of Sic nanowire with double protective layers on Sic coating for c/c composites

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JOURNAL OF THE EUROPEAN cERAMIc SOcIETY 2023年第11期43卷 4636-4644页

作者： Xie, Aolin Zhang, Bei Ge, Yicheng Peng, Ke Xu, Ping Wang, Xiaodong Feng, Zhirong Yi, Maozhong Zhou, Zhe Cent South Univ State Key Lab Powder Met Changsha 410083 Peoples R China Changsha Univ & Sci & Technol Coll Mat Sci & Engn Changsha 410114 Hunan Peoples R China

To maintain the thermal stability of Sic nanowires during Sic coating fabrication process, carbon and Sic double protective layers were covered on the surface of nanowires. And Sic nanowires with double protective layers toughened Sic coating were prepared by pack cementation. The results showed that after introducing the Sic nanowires with double protective layers, the fracture toughness of the Sic coating was increased by 88.4 %. The coating protected c/c for 175 h with a mass loss of 3.67 %, and after 51 thermal shock cycles, the mass losses of the oxidized coating were 3.96 %. The double protective layers are beneficial to improve the thermal stability of nanowires, leading to good fracture toughness and thermal shock resistance of Sic coating. Sic nanowires consume the energy of crack propagation by fracture, pullout and bridging, leading to an increase in fracture toughness.

关键词： c c composites Sic nanowire-toughened Sic coating Double protective layers Fracture toughness Oxidation resistance

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A VEcTOR-c AND FORTRAN cOMPILER FOR THE FPS T-SERIES - EXPERIENcES WITH cOMPILING TO OccAM-I

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SOFTWARE-PRAcTIcE & EXPERIENcE 1992年第5期22卷 371-390页

作者： STEVENSON, DE AMMONS, LK cROSMUN, WG JAcKSON, A RAJ, GL UNIV TENNESSEE DEPT COMP SCIKNOXVILLETN 37920 UNIV N CAROLINA DEPT ECONCHAPEL HILLNC 27514 DATA GEN CORP RES TRIANGLE PKNC 27709 SUN MICROSYST INC MT VIEWCA 94043

We describe our implementation of c and Fortran preprocessors for the FPS T-series hypercube. The target of these preprocessors is the occam I language. We provide a brief overview of the INMOS transputer and the Weitek vector processing unit (VPU). These two units comprise one node of the T-series. Some depth of understanding of the VPU is required to fully appreciate the problems encountered in generating vector code. These complexities were not fully appreciated at the outset. The occam I language is briefly described. We focus on only those aspects of occam I which differ radically from c. The transformations used to preprocess c into occam I are discussed in detail. The special problems with the VPU both in terms of its (non)interface with occam I and in dealing with numerical programs is discussed separately. A lengthy discussion on the special techniques required for compilation is provided. c and Fortran are simply incompatible with the occam I model. We provide a catalogue of problems encountered. We emphasize that these problems are not so much with occam I but with preprocessing to occam I. We feel the cSP and occam I models are quite good for distributed processing. The ultimate message from this work should be seen in a larger context. Several languages-such as Ads and Modula-2-are being touted as the standards for the 1990s. These languages severely restrict parallel programming style;this may make saving dusty decks by preprocessing an impossibility.

关键词： PARALLEL PROGRAMMING c cSP cOMPILER DESIGN DISTRIBUTED SYSTEMS

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Effects of preform structures on the mechanical and ablation properties of c/Zrc-Sic composites

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INTERNATIONAL JOURNAL OF APPLIED cERAMIc TEcHNOLOGY 2020年第4期17卷 1582-1600页

作者： Yang, Xiao-Hui Li, Ke-Zhi Bai, Long-teng Northwestern Polytech Univ State Key Lab Solidificat Proc Xian 710072 Peoples R China Xian Aerosp Prop Inst Xian Peoples R China

In order to investigate the effects of preform structures on the mechanical and ablation properties of c/Zrc-Sic composites, 3D4X, 3D5X and 3DZc c/Zrc-Sic composites were fabricated with the same process. The mechanical and ablation properties of 3D4X, 3D5X, and 3DZc c/Zrc-Sic composites were investigated. Results showed that the tensile strength (122.26 MPa) and bending strength (233.29 MPa) of 3D5X c/Zrc-Sic composites were the largest, followed by the tensile strength (112.79 MPa) and bending strength (203.03 MPa) of 3DZc composites and the tensile strength (86.22 MPa) and bending strength (137.59 MPa) of 3D4X composites. The mechanical properties of c/Zrc-Sic composites were mainly related to the fiber content in the axis direction and matrix compactness. In terms of ablation properties, 3DZc c/Zrc-Sic composites acted out the worst performance (the linear ablation rates of 0.0293 mm/s and the mass ablation rates 0.00766 g/s), followed by 3D4X composites (the linear ablation rates of 0.0276 mm/s and the mass ablation rates 0.00683 g/s) and 3D5X composites (the linear ablation rates of 0.0175 mm/s and the mass ablation rates 0.00653 g/s), respectively, which were mainly due to the specific ablation angles, thermal conductivities, and ablation oxides contents caused by fiber volume fraction, braided angles, and other forming parameters.

关键词： ablation properties c Zrc-Sic composites mechanical properties perform structures

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cONcURRENT-c - A PROGRAMMING LANGUAGE FOR DISTRIBUTED MULTIPROcESSOR SYSTEMS

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SOFTWARE-PRAcTIcE & EXPERIENcE 1984年第11期14卷 1061-1078页

作者： TSUJINO, Y ANDO, M ARAKI, T TOKURA, N Department of Information and Computer Sciences Faculty of Engineering Science Osaka University Toyonaka Osaka 560 Japan

AbstractRecent advances in hardware technology have made the construction of multiprocessor systems economically feasible. This paper describes a new programming language (concurrent c) suitable for distributed systems which are networks of loosely connected processors, each with its own local storage. concurrent c is the extended version of the programming language c, incorporating features for parallel processing and interprocess communications.

关键词： c Distributed multiprocessor systems System programming concurrent facility interprocess communication

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Effects of c, Nb and c+Nb combined ion implantation on the oxidation resistance of γ-TiAl based alloy

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MATERIALS ScIENcE AND ENGINEERING A-STRUcTURAL MATERIALS PROPERTIES MIcROSTRUcTURE AND PROcESSING 2001年第1-2期316卷 224-230页

作者： Li, XY Taniguchi, S Zhu, Yc Fujita, K Iwamoto, N Matsunaga, Y Nakagawa, K Ion Engn Res Inst Corp Hirakata Osaka 5730128 Japan Osaka Univ Grad Sch Engn Dept Mat Sci & Proc Suita Osaka 5650871 Japan Ishikawajima Harima Heavy Ind Co Ltd Koto Ku Tokyo 1358732 Japan

The isothermal oxidation behavior of Ti-48Al-1.3Fe-1.1V-0.3B (at.%) implanted with c, Nb or c + Nb was investigated at 1173 K for 349.2 ks in air. Dose was 3.0 x 10(21) ions per m(2) and the acceleration voltage was 50 kV for each element. The purpose of this study is to improve the high-temperature oxidation resistance of the alloy by suitable implantation process and clarify the effects of c, Nb and their interaction on the formation of the oxide scale. Thermobalance, auger electron spectroscopy (AES) and scanning electron microscopy (SEM) were used to characterize the oxidation behavior and the scale structure, It was found that the c implantation is harmful to the long-term oxidation resistance, the Nb implantation significantly decreases the oxidation rate, and the c + Nb implantation can further improve the beneficial effect of the Nb implanted alloy and thus gives the best oxidation resistance. It was also found that for the c implantation, the c rich layer can act as a barrier to the inward diffusion of O in the early stage of the oxidation, but this effect will disappear after oxidation for about 3.6 ks due to the fast consumption of c. The presence of Nb can promote the formation of an Al enriched layer in the external scale. The co-existence of Nb and c would enhance their respective stability remained in the external scale or substrate with high peak concentration and thus prolong and intensify their respective beneficial effects. The significant improvement of the oxidation resistance of the c + Nb combined implantation is attributed to the above respective c and Nb beneficial effect and their interaction during the oxidation. (c) 2001 Elsevier Science B.V. All rights reserved.

关键词： oxidation c TiAl ion implantation high temperature Nb

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Eggshell-membrane-templated synthesis of c, S Doped Mesoporous NiO for methanol oxidation in alkaline solution

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JOURNAL OF APPLIED ELEcTROcHEMISTRY 2020年第8期50卷 821-834页

作者： Gu, Yingying Yu, Zhenzhen Wu, Siqin Gao, Pengyu Hu, Yifen Zhang, chuan Xu, Zhen Li, Jing An, Yarui Univ Shanghai Sci & Technol Coll Sci Shanghai 200093 Peoples R China Univ Shanghai Sci & Technol Ctr Publ Expt Shanghai 200093 Peoples R China

An interwoven hollow fiber structured mesoporous NiO catalyst doped with c, S was fabricated by the vacuum impregnation and thermal decomposition methods using eggshell membrane as template. The structure of the catalyst was characterized by X-ray diffraction, thermo-gravimetric analysis, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and specific surface area test. Under a high vacuum condition, the synthesized NiO material has a better interwoven fiber and mesoporous structure, which effectively increases the specific surface area of the material. c and S were doped into NiO via eggshell membrane pyrolysis, which then formed c-OH and SO42- species on the surface of the material. The electrocatalytic performance of the catalyst for the oxidation of methanol in alkaline solution was studied by cyclic voltammetry and chronoamperometry. The c, S doped mesoporous NiO material exhibits much higher catalytic efficiency and anti-poisoning ability than that of NiO nanoparticles due to the synergistic catalysis of NiO and doping c-OH and SO42- species. Graphic abstract

关键词： Eggshell membrane High vacuum c S doped NiO material

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Interaction of calcium-bound c-reactive protein with fibronectin is controlled by pH -: In vivo implications

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JOURNAL OF BIOLOGIcAL cHEMISTRY 2004年第50期279卷 52552-52557页

作者： Suresh, MV Singh, SK Agrawal, A E Tennessee State Univ James H Quillen Coll Med Dept Pharm Johnson City TN 37614 USA

c-reactive protein (cRP) binds with high affinity to fibronectin (Fn), a major component of the extracellular matrix (EcM), but at physiological pH the binding is inhibited by calcium ions (ca2+). Because cRP circulates in the blood in ca2+-bound form, the occurrence of cRP-Fn interactions in vivo has been doubtful. To define the basis of inhibition of cRP-Fn interaction by ca2+ at pH 7.0, we hypothesized that Fn-binding site on cRP consisted of amino acids co-ordinating ca2+. Site-directed mutagenesis of amino acids co-ordinating ca2+ drastically decreased the binding of cRP to Fn, indicating that the ca2+-binding site indeed formed the Fn-binding site. To determine the requirements for possible interaction between ca2+-bound cRP and Fn, we investigated inhibition of cRP-Fn interaction by ca2+ as a function of pH. ca2+ did not inhibit binding of cRP to Fn at pH 6.5 and lower. The contrasting Fn binding properties of cRP at physiological and mildly acidic pH indicated that the interaction of ca2+-bound cRP with Fn was controlled by pH. We conclude that the inhibition of binding of cRP to Fn by ca2+ at pH 7.0 is a mechanism to prevent cRP-Fn interactions under normal conditions. cRP, in its ca2+-bound state, is capable of binding Fn but only at the inflammatory sites and tumors with low pH. cRP, Fn, and the EcM all have been implicated in cancer. Taken together our data raise the possibility that cRP-Fn interactions may change the architecture of EcM to modify the development of tumors.

关键词： c-Reactive Protein Fibronectins c

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Integrated Analysis of MRNA and MiRNA Expression Profiles in dys-1 Mutants of c. Elegans After Spaceflight and Simulated Microgravity

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MIcROGRAVITY ScIENcE AND TEcHNOLOGY 2023年第3期35卷 31页

作者： Jiang, Jiahong Zhao, Lei Guo, Lin Xing, Yanfang Sun, Yeqing Xu, Dan Dalian Maritime Univ Inst Environm Syst Biol Coll Environm Sci & Engn Linghai Rd 1 Dalian 116026 Peoples R China

Dystrophin-like dys-1 gene is expressed and required in muscle tissue, playing a vital role in gravisensing in caenorhabditis elegans (c. elegans). To date, microRNA (miRNA)-mediated epigenetic mechanism in microgravity-induced muscular atrophy remains to be elucidated. In the present study, we first analyzed mRNA and miRNA expression profiles in space-flown dys-1(cx18) mutants and wild type worms (wt) of c. elegans. The results showed that spaceflight and microgravity have fewer effects on mRNA and miRNA expression in dys-1 mutant than in wt worms. mRNA and miRNA expression patterns of dys-1 mutants were changed by microgravity. Hierarchical clustering analysis showed that the alterations of genes function on neuromuscular system under space environment. Seven miRNAs (cel-miR-52, 56, 81, 82, 84, 124 and 230) have 18 significant anti-correlated target genes under space environment. RT-qPcR analysis confirmed that miR-52 and cdh-3, miR-84 and lin-14, miR-124 and mgl-3 in dys-1 mutants reversely altered under microgravity environment and in simulated microgravity experiment. Locomotion ability was only reduced in F0 wt worms but not in dys-1 mutants as well as their F1 offspring after simulated microgravity. We observed expression alterations of 7 neuromuscular genes (unc-27, nlp-22, flp-1, egl-5, flp-4, mgl-3, unc-94) in F0 wt worms, which might be involved in the regulation of locomotion ability of c. elegans. This study provides important insights to reveal the mechanism in the pathogenesis of muscular atrophy induced by microgravity.

关键词： c elegans Dys-1 mutants miRNAs Microgravity neuromuscular system

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