检索结果-内蒙古大学图书馆

NOB1 is essential for the survival of RKO colorectal cancer cells

World Journal of Gastroenterology 2015年第3期21卷 868-877页

作者： Xiao-Wen He Tao Feng Qiao-Ling Yin Yuan-Wei Jian Ting Liu Department of General Surgery Shaoxing People’s Hospital Shaoxing Hospital of Zheijiang University Department of Gastroenterology Xiangya Hospital of Central South University

AIM:To determine the role of NOB1,a regulator of cell survival in yeast,in human colorectal cancer ***:Lentivirus-mediated small interfering RNA(si RNA) was used to inhibit NOB1 expression in RKO human colorectal cancer cells in vitro and in vivo in a mouse xenograft *** in vitro and in vivo knockdown efficacy was determined using both Western blot and quantitative reverse transcription polymerase chain reaction(q RT-PcR).q RT-PcR was also used to analyze the downstream signals following NOB1 *** growth and colony formation assays were used to determine the effect of NOB1 inhibition on RKO proliferation and their ability to form *** activity,as evaluated by terminal deoxytransferase-mediated d UTP nick end labeling(TUNEL),and annexin V staining were used to determine the presence of apoptotic cell death prior to and following NOB1 *** cycle analysis was used to determine the effect of NOB1 inhibition on RKO cell cycle.A c DNA microarray was used to determine global differential gene expression following NOB1 ***:Virus-mediated si RNA inhibition of NOB1 resulted in(1) the down-regulation of NOB1 expression in RKO cells for both the m RNA and protein;(2) inhibition of NOB1 expression both in vitro and in vivo experimental systems;(3) cell growth inhibition via significant induction of cell apoptosis,without alteration of the cell cycle distribution; and(4) a significant decrease in the average weight and volume of xenograft tumors in the NOB1-si RNA group compared to the control scr-si RNA group(P = 0.001,P < 0.05).Significantly more apoptosis was detected within tumors in the NOB1-si RNA group than in the control *** analysis detected 2336 genes potentially regulated by *** of these genes are associated with the WNT,cell proliferation,apoptosis,fibroblast growth factor,and angiogenesis signaling pathways,of which BAX and WNT were validated by q *** them,1451 probes,repr

关键词： NOB1 Small RNA interference Apoptosis colorectal c

来源：评论

学校读者我要写书评

暂无评论

Burial dolomitization, the genesis of dolomite in the Dapu Formation (Upper carboniferous), Guixinan area, Youjiang basin, Southwest china: petrologic and geochemical evidence

引用

cARBONATES AND EVAPORITES 2020年第2期35卷 1-14页

作者： Miao, Zhuowei Gong, Enpu Zhang, Yongli Guan, changqing Huang, Wentao Northeastern Univ Resources & Civil Engn Coll Shenyang 110819 Liaoning Peoples R China

The genesis of dolomite is an intensively debated and extensively studied problem in the field of carbonate sedimentology. This study focuses on the dolomite of the Dapu Formation in the Guixinan area, with the aim of deciphering the mechanism of dolomitization in the Dapu Formation. Three types of sedimentary environment, including the open marine of inner ramp, the restricted marine of inner ramp, and the shoal of inner ramp, have been identified. Three types of dolostone (DI, DII and DIII) identified in the study area with massive structure had close relationship with fault structures, and contacted with limestone in curved diffuse way on the outcrop. Both of the above points indicated diagenetic dolomite. Three types of dolomite (D1, D2 and D3) displayed similar dark-light purple red luminescence, indicating that the characteristics of their diagenetic fluids were similar. The planar-s to nonplanar dolomite crystals indicated that the temperature was more than 50 degrees c. The mineral assemblage including D2, D3, and silicate minerals (feldspar, tremolite and actinolite) suggested contact metamorphism and tht the diagenetic fluid was rich in silicon and magnesium. The dolomitization process was not influenced by the leaching of meteoric water in surface or shallow buried environment and hydrothermal fluids, based on the corrected REE characteristics. The range of the delta O-18 of dolostone samples showed that the burial dolomitization had taken place in high-temperature environment. The lower Sr-87/Sr-86 ratio of the dolostone samples than the coeval seawater meant that the diagenetic fluid was derived from the alteration of mid-ocean ridge basalt. Based on the geologic setting, it was speculated that the burial dolomitization was driven by the hydro-tectonic processes in the passive margin burial diagenesis hydrologic realm. In brief, magnesium came from the low-temperature alteration of ocean basalts, the thermohaline and compactional flow supplied hydrodyna

关键词： c O Sr isotopes Burial dolomitization Dolomite Dapu Formation Passive margin Rare earth elements

来源：评论

学校读者我要写书评

暂无评论

PAPAIN-DERIVED FRAGMENT-IIc OF TETANUS TOXIN - ITS BINDING TO ISOLATED SYNAPTIc-MEMBRANES AND RETROGRADE AXONAL-TRANSPORT

引用

BRAIN RESEARcH 1981年第1-2期210卷 291-299页

作者： BIZZINI, B GROB, P AKERT, K UNIV ZURICH INST BRAIN RESCH-8029 ZURICHSWITZERLAND INST PASTEUR F-92420 MARNES LA COQUETTFRANCE

The papain-derived fragment II(c) of tetanus toxin, which is immunologically identical to the B-II(b) fragment, has previously been shown to bind gangliosides. As could be expected from its analogy with the B-II(b) fragment, the II(c) fragment was also found to bind to isolated synaptic membranes and to be transported retrogradely from the axonal endings within muscle to the motorneuronal perikarya. It is concluded that the II(c) fragment - like the B-II(b) fragment - might also serve as a specific carrier for chemical and chemotherapeutical agents into the central nervous system.

关键词： tetanus toxin retrograde axonal transport II c fragment synaptic membranes

来源：评论

学校读者我要写书评

暂无评论

The Acid-Dependent and Independent Effects of Lactobacillus acidophilus cL1285, Lacticaseibacillus casei LBc80R, and Lacticaseibacillus rhamnosus cLR2 on clostridioides difficile R20291

引用

PROBIOTIcS AND ANTIMIcROBIAL PROTEINS 2021年第4期13卷 949-956页

作者： Gunaratnam, Sathursha Diarra, carine Paquette, Patrick D. Ship, Noam Millette, Mathieu Lacroix, Monique Ctr Armand Frappier Sante Biotechnol Inst Natl Rech Sci 531 Blvd Prairies Laval PQ H7V 1B7 Canada BioK Plus Int Inc 495 Blvd Armand Frappier Laval PQ H7V 4B3 Canada

clostridioides difficile infections (cDI) result from antibiotic use and cause severe diarrhea which is life threatening and costly. A specific probiotic containing Lactobacillus acidophilus cL1285, Lacticaseibacillus casei LBc80R, and Lacticaseibacillus rhamnosus cLR2 has demonstrated a strong inhibitory effect on the growth of several nosocomial c. difficile strains by production of antimicrobial metabolites during fermentation. Though there are several lactobacilli shown to inhibit c. difficile growth by processes relying on acidification, this probiotic has demonstrated potency for cDI prevention among hospitalized patients. Here, we describe the acid-dependent and independent mechanisms by which these strains impair the cytotoxicity of a hypervirulent strain, c. difficile R20291 (cD). These bacteria were co-cultured in a series of experiments under anaerobic conditions in glucose-rich and no-sugar medium to inhibit or stimulate cD toxin production, respectively. In glucose-rich medium, there was low cD toxin production, but sufficient amounts to cause cytotoxic damage to human fibroblast cells. In co-culture, there was acidification by the lactobacilli resulting in growth inhibition as well as >= 99% reduced toxin A and B production and no observable cytotoxicity. In the absence of glucose, cD produced much more toxin. In co-culture, the lactobacilli did not acidify the medium and cD growth was unaffected;yet, the amount of detected toxin A and B was decreased by 20% and 41%, respectively. Despite the high concentration of toxin, cells exposed to the supernatant from the co-culture were able to survive. These results suggest that in addition to known acid-dependent effects, the combination of L. acidophilus cL1285, L. casei LBc80R, and L. rhamnosus cLR2 can interfere with cD pathogenesis without acidification: (1) reduced toxin A and B production and (2) toxin neutralization. This might explain the strain specificity of this probiotic in potently preventing c.

关键词： c difficile Lactobacillus Probiotic Antibiotic Infection Pathogenesis microbiota colonization resistance Infection control Preventive intervention L acidophilus cL1285 L casei LBc80R L rhamnosus cLR2

来源：评论

学校读者我要写书评

暂无评论

EXPRESSION OF THE MULTIDRUG RESISTANcE GENE IN MYELOID LEUKEMIAS

引用

LEUKEMIA RESEARcH 1990年第1期14卷 11-+页

作者： SATO, H GOTTESMAN, MM GOLDSTEIN, LJ PASTAN, I BLOcK, AM SANDBERG, AA PREISLER, HD BARRETT CTR CANC PREVENT RES & TREATMENT 234 GOODMAN AVE CINCINNATI OH 45267 USA

The human multidrug-resistance gene (MDR1) encodes an energy-dependent multidrug efflux protein responsible for the cross-resistance of cultured cells to natural product chemotherapeutic agents such as the anthracyclines and vinca alkaloids. RNA transcript levels were measured in leukemia cells obtained from 15 adult acute nonlymphotic leukemia (ANLL) cases and 15 cases of chronic myelogenous leukemia (cML). Expression of MDR1 RNA was common in ANLL, and appears to be most frequent in leukemic cells of patients with the poorest response to chemotherapy. Expression of the MDR1 gene was not detectable in the peripheral white blood cells of any of the cML cases during the chronic phase, but was detectable in the immature cells present during this phase of the disease. The cells of the three blastic crisis patients contained detectable levels of MDR1 RNA. These studies support the idea that expression of the MDR1 gene contributes to drug resistance in ANLL, and may play a role in some instances in the drug-resistance of cML in blastic crisis. In contrast, studies of the level of expression of anionic glutathione transferase and DNA polymerase B failed to show any relationship between the RNA transcript levels of these enzymes and responsiveness to chemotherapy.

关键词： Multidrug resistance AML cML HL , HL-60 Nc , RNS from nonresistant chinese hamster ovary cell line AUXB1 Pc , RNA from colcicine-resistant chinese hamster ovary cell line MDR1 , multidrug resistant gene GST-P , anionic glutathione transferase H 4, histone H4 B-PLM , DNA polymerase M , bone marrow specimen P , peripheral blood specimen c -mye, proto-oncogene c- myc TPI , triose phosphate isomerate

来源：评论

学校读者我要写书评

暂无评论

caveolin-1 as a possible target in treatment for acne

引用

EXPERIMENTAL DERMATOLOGY 2020年第2期29卷 177-184页

作者： Kruglikov, Ilja L. Scherer, Philipp E. Wellcomet GmbH Sci Dept Karlsruhe Germany Univ Texas Southwestern Med Ctr Dallas Touchstone Diabet Ctr Dept Internal Med Dallas TX 75390 USA

Expression of caveolin-1 (cav-1) is an important pathophysiological factor in acne. cav-1 strongly interacts with such well-recognized etiopathogenic factors such as hyperseborrhea, follicular hyperkeratinization and pathogenicity of cutibacterium acnes. cav-1 is a strong negative regulator of transforming growth factor beta (TGF-beta) expression. It acts as a critical determinant of autophagy, which is significantly induced in acne lesions through c. acnes and by absorption of fatty acids. cav-1 also demonstrates different correlations with the development of innate immunity. We propose that normalization of cav-1 expression can serve as a target in anti-acne therapy.

关键词： acne autophagy c acnes caveolin follicular hyperkeratinization hyperseborrhea innate immunity pathophysiology

来源：评论

学校读者我要写书评

暂无评论

Experimental apparatus for critical current measurement above 5 K using Bi-based oxide current leads

引用

IEEE TRANSAcTIONS ON APPLIED SUPERcONDUcTIVITY 1997年第2期7卷 707-710页

作者： Hasebe, T Mitsubori, H Sakuraba, J Ishihara, M Awaji, S Watanabe, K TOHOKU UNIV INST MAT RESAOBA KUSENDAIMIYAGI 98077JAPAN

An experimental apparatus for the measurement of temperature dependence of critical current within a strong magnetic field has been developed. Samples, which are tested by the four probe method, are cooled by a Gifford-McMahon (GM) type cryocooler to the lowest temperature of 5K. The sample holder has a diameter of 70 mm and a height of 70 mm. Transport currents, up to 150 A, is supplied to two samples through Bi-based oxide superconducting current leads, An external magnetic field of up to 15 T, which is applied to the samples perpendicularly to sample axis, is generated by a water cooled single Bitter magnet with a room temperature bore of 82 mm, As a performance test, the critical current properties of a silver clad Bi-based oxide tape have been measured at 5.5 K, 30 K and 50 K within a magnetic field up to 15 T. This experiment has demonstrated the validity of this experimental apparatus.

关键词： bismuth compounds critical currents cryostats electric current measurement high-temperature superconductors probes superconducting cables 15 T 30 K 5.5 K 50 K 82 mm Bi-based oxide superconducting current leads Bitter magnet critical current measurement c Superconducting cables Electric current measurement experimental devices Probes cryostats four probe method bismuth compounds temperature dependence critical currents current measuring high-temperature superconductors exterior magnetic field

来源：评论

学校读者我要写书评

暂无评论

centaurea bruguieranainhibits cell proliferation, causes cell cycle arrest, and induces apoptosis in human McF-7 breast carcinoma cells

引用

MOLEcULAR BIOLOGY REPORTS 2020年第8期47卷 6043-6051页

作者： Nasr, Fahd A. Shahat, Abdelaaty A. Alqahtani, Ali S. Ahmed, Mohammad Z. Qamar, Wajhul Al-Mishari, Abdullah A. Almoqbil, Abdulaziz N. King Saud Univ Coll Pharm Med Aromat & Poisonous Plants Res Ctr Riyadh 11451 Saudi Arabia King Saud Univ Dept Pharmacognosy Coll Pharm Riyadh 11451 Saudi Arabia Natl Res Ctr Chem Med Plants Dept 33 El Bohouth StPOB 12622 Giza Egypt King Saud Univ Coll Pharm Cent Lab Riyadh 11451 Saudi Arabia

centaurea bruguierana, of theAsteraceaefamily, has a long history of use in traditional medicines for the treatment of various ailments. However, the anticancer activity and underlying mechanisms have not yet been assessed. Thec. bruguieranawas extracted with methanol and fractionated into four different fractions. Different cancer cells and one non-cancerous were used to examine the cytotoxic effects of these fractions using MTT assay. The most potent fraction,c. bruguieranaethyl acetate fraction (cB EtOAc), was explored for its effects on cell cycle progression and apoptosis induction by Hoechst staining and annexin V-PI double staining in McF-7 cells. The expression of apoptosis-related genes was quantified by RT-PcR. Of all fractions, cB EtOAc was found to have the strongest antiproliferative activity (Ic50 = 10 mu g/mL) against McF-7 cells. The antiproliferative activity of the cB EtOAc fraction against McF-7 was correlated with arrested of cell cycle in the G1 phase, nuclear fragmentation, and the exposure of phosphatidylserine. The induction of apoptosis by cB EtOAc in McF-7 cells was also associated with an increase in the Bax/Bcl-2 ratio and higher expression of caspases. Overall, our results demonstrated that cB EtOAc showed apoptosis-inducing effects, suggesting thatc. bruguieranamay be a promising source for a novel chemotherapeutic agents for the treatment of breast cancer.

关键词： c bruguierana cell cycle Apoptosis Bcl-2 Bax McF-7

来源：评论

学校读者我要写书评

暂无评论

cHEMIcAL-REAcTIVITY OF METALLOPROTEINS IN cONFORMATIONALLY OUT-OF-EQUILIBRIUM STATES

引用

BIOcHIMIcA ET BIOPHYSIcA AcTA 1979年第3-4期549卷 255-280页

作者： BLUMENFELD, LA DAVIDOV, RM Institute of Chemical Physics Academy of Science of the U.S.S.R. Moscow U.S.S.R.

来源：评论

学校读者我要写书评

暂无评论

Homologous complement activation on drug-induced apoptotic cells from a human lung adenocarcinoma cell line

引用

IMMUNOBIOLOGY 1997年第5期196卷 491-503页

作者： Hara, T Matsumoto, M Tsuji, S Nagasawa, S Hiraoka, A Masaoka, T Kodama, K Horai, T Sakuma, T Seya, T CTR ADULT DIS DEPT IMMUNOL & CELL BIOLOSAKA 537JAPAN CTR ADULT DIS DEPT HEMATOLOSAKA 537JAPAN HOKKAIDO UNIV DEPT PHARMACEUT SCISAPPOROHOKKAIDO 060JAPAN JRDC PRESTOKYOTOJAPAN

Activation of the alternative pathway of homologous complement (c) was observed in a human lung adenocarcinoma cell line, cADO 43, after the cells had become apoptotic following treatment in vitro With vincristine and predonisolone. Deposition of c3b and c3bi on the serum-treated apoptotic cells was revealed by flow cytometry with anti-c3b and -c3bi-specific antibodies and immunoblotting with anti-c3 antibody immunoprecipitates extracted from solubilized fractions of serum-treated apoptotic cells. Two molecular mechanisms were found to be responsible for this post-apoptotic c-activation. Firstly, all c regulators, decay accelerating factor (DAF), membrane cofactor protein (McP) and c3b/c4b receptor (cRI), were diminished on the cell surface concomitantly with the apoptotic process. Secondly, unidentified molecules which potentially activate homologous c and accept c3b/c3bi fragments became expressed on the cell surface during the apoptotic process. These findings may explain the mechanism whereby tumor cells are efficiently eliminated through chemotherapy.

关键词： c complement cRI c3b/c4b receptor cR2 c3d/Epstein-Barr virus receptor DAF decay accelerating factor (cD55) GPI glycanphosphatidylinositol McP membrane cofactor protein (cD46) NHS normal human serum PBS phosphate buffered saline PI propidium iodide TdT terminal deoxytransferase

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：