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Full-field dual-color 100-nm super-resolution imaging reveals organization and dynamics of mitochondrial and ER networks

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OPTICS EXPRESS 2013年第22期21卷 26162-26173页

作者： Brunstein, Maia Wicker, Kai Herault, Karine Heintzmann, Rainer Oheim, Martin CNRS UMR 8154 F-75006 Paris France INSERM U603 F-75006 Paris France Univ Paris 05 PRES Sorbonne Paris Cite F-75006 Paris France Inst Photon Technol D-07745 Jena Germany Univ Jena Inst Phys Chem Abbe Ctr Photon D-07743 Jena Germany Kings Coll London NHH Randall Div Cell & Mol Biophys London SE1 1UL England

Most structured illumination microscopes use a physical or synthetic grating that is projected into the sample plane to generate a periodic illumination pattern. Albeit simple and cost-effective, this arrangement hampers fast or multi-color acquisition, which is a critical requirement for time-lapse imaging of cellular and sub-cellular dynamics. In this study, we designed and implemented an interferometric approach allowing large-field, fast, dual-color imaging at an isotropic 100-nm resolution based on a sub-diffraction fringe pattern generated by the interference of two colliding evanescent waves. Our all-mirror-based system generates illumination patterns of arbitrary orientation and period, limited only by the illumination aperture (NA = 1.45), the response time of a fast, piezo-driven tip-tilt mirror (10 ms) and the available fluorescence signal. At low mu W laser powers suitable for long-period observation of life cells and with a camera exposure time of 20 ms, our system permits the acquisition of super-resolved 50 mu m by 50 mu m images at 3.3 Hz. The possibility it offers for rapidly adjusting the pattern between images is particularly advantageous for experiments that require multi-scale and multi-color information. We demonstrate the performance of our instrument by imaging mitochondrial dynamics in cultured cortical astrocytes. As an illustration of dual-color excitation dual-color detection, we also resolve interaction sites between near-membrane mitochondria and the endoplasmic reticulum. Our TIRF-SIM microscope provides a versatile, compact and cost-effective arrangement for super-resolution imaging, allowing the investigation of co-localization and dynamic interactions between organelles - important questions in both cell biology and neurophysiology (C) 2013 Optical Society of America

关键词： Diffractive optical elements Evanescent waves image processing algorithms Spatial light modulators Structured illumination microscopy Total internal reflection

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Optimization of Illumination Frequency and Preclinical Validation of a Wide-field Structured Illumination Microscope Designed for Imaging in situ Tumor Margins

Optimization of Illumination Frequency and Preclinical Valid...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Fu, Henry L. Mueller, Jenna L. Javid, Melodi Kirsch, David G. Ramanujam, Nimmi Brown, J. Quincy Duke Univ Dept Biomed Engn Durham NC 27708 USA Duke Univ Sch Med Dept Pharmacol & Canc Biol Durham NC 27710 USA Duke Univ Sch Med Dept Radiat Oncol Durham NC 27710 USA Tulane Univ Dept Biomed Engn New Orleans LA USA

We present a widefield structured illumination microscope for imaging surgical tumor margins in situ. The impact of frequency and turbidity on optical section thickness and SNR was characterized to determine the optim... 详细信息

ISBN: (纸本)9781557529732

关键词： Biological imaging Illumination design image processing algorithms Multiple scattering Spatial light modulators Structured illumination microscopy Fluorescence microscopy (170.2520) Medical and biological imaging (170.3880) Medical optics instrumentation (170.3890)

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Simulation of a zoom lens with Wavefront Coding using various methods of deconvolution

Simulation of a zoom lens with Wavefront Coding using variou...

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Frontiers in Optics, FIO 2013

作者： Dufouleur, Paul Barritault, Pierre Lepine, Thierry CEA LETI MINATEC Campus DOPT 17 rue des martyrs 38054 Grenoble CEDEX 9 France Université de Lyon Université Jean Monnet 42000 Saint-Etienne France Institut d'Optique Rhône Alpes 18 rue B. Lauras 42000 Saint-Etienne France

We design a single moving element zoom lens using wavefront coding. We study the impact of different types of deconvolution in order to optimize the image, according to a visual metric. © OSA 2013.

ISBN: (纸本)9781557529879

We design a single moving element zoom lens using wavefront coding. We study the impact of different types of deconvolution in order to optimize the image, according to a visual metric. © OSA 2013.

关键词： Deconvolution image processing image processing algorithms image quality Wavefront encoding Zoom lenses Lens system design (080.3620) Metrics (110.3925) Wavefront encoding (110.7348)

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image processing Technology of Flaws within Infrared Transmitting Glasses

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Journal of Wuhan University of Technology(Materials Science) 2012年第6期27卷 1180-1183页

作者：钟忺 YUAN Jingling 陶海征 LIU Yonghua ZU Chengkui School of Computer Science and Technology Huazhong University of Science and&Technology State Key Laboratory of Silicate Materials for Architectures Wuhan University of Technology Institute of Quaru and Special Glasses China Building Materials AcademyBeijing 100024China

We investigated image processing algorithms of the original infrared glass flaw image. Using the Laplacian edge enhancement following LSD （Line Segment Detector） algorithm, we can get a good flaw image very consistent with the original one. This study is very helpful to further enhance the infrared glass flaw inspection technique.

关键词： infrared transmitting materials image processing algorithms flaw image

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High precision two-step calibration method for the fish-eye camera

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APPLIED OPTICS 2013年第7期52卷 C37-C42页

作者： Tu, Bo Liu, Lu Liu, Yihui Jin, Ye Tang, Junxiong Peking Univ Sch Elect Engn & Comp Sci State Key Lab Adv Opt Commun Syst & Networks Beijing 100871 Peoples R China

Fish-eye cameras are widely used on many occasions due to their ultrawide field of view (about 180 degrees). In this paper, we present a high-precision two-step calibration method to calibrate fish-eye cameras. The two steps are the global polynomial projection model fitting and local line-fitting calibration optimization. In the first step, we obtain the projection model of the fish-eye camera and apply a quartic polynomial to fit the projection model over the entire image. In the second step, the fish-eye image is partitioned into several sections and line fitting is adopted in each section in order to further reduce the residual error of the first calibration step. Experiments show that the new method is able to correct the distortion of the real scene image well. In addition, its average reprojection error is 0.15 pixel better than 0.40 pixel of the general projection model described. The reason that higher calibration precision is obtained is that this method not only considers the global projection model of the fish-eye camera but also considers the local characteristics, such as small tangential distortion and asymmetry. (C) 2013 Optical Society of America

关键词： Camera calibration Distortion image metrics image processing algorithms Optical wireless communication Virtual reality

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Open-access operating algorithms for commercial videokeratographer and improvement of corneal sampling

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APPLIED OPTICS 2013年第7期52卷 C24-C29页

作者： Espinosa, Julian Mas, David Perez, Jorge Belen Roig, Ana Univ Alicante IUFACyT Alicante 03690 Spain Univ Alicante Dept Opt Farmacol & Anat Alicante 03690 Spain

We present an algorithm to process images of reflected Placido rings captured by a commercial videokeratoscope. Raw data are obtained with no Cartesian-to-polar-coordinate conversion, thus avoiding interpolation and associated numerical artifacts. The method provides a characteristic equation for the device and is able to process around 6 times more corneal data than the commercial software. Our proposal allows complete control over the whole process from the capture of corneal images until the computation of curvature radii. (C) 2013 Optical Society of America

关键词： image processing image processing algorithms image quality Optical surfaces Temporal processing Temporal resolution

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image formation analysis and high resolution image reconstruction for plenoptic imaging systems

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APPLIED OPTICS 2013年第10期52卷 D22-D31页

作者： Shroff, Sapna A. Berkner, Kathrin Ricoh Innovat Inc Computat Opt Sensing & Visual Proc Res Menlo Pk CA 94025 USA

Plenoptic imaging systems are often used for applications like refocusing, multimodal imaging, and multiview imaging. However, their resolution is limited to the number of lenslets. In this paper we investigate paraxial, incoherent, plenoptic image formation, and develop a method to recover some of the resolution for the case of a two-dimensional (2D) in-focus object. This enables the recovery of a conventional-resolution, 2D image from the data captured in a plenoptic system. We show simulation results for a plenoptic system with a known response and Gaussian sensor noise. (C) 2013 Optical Society of America

关键词： image formation theory image processing image processing algorithms Imaging systems Multimodal imaging Multispectral imaging

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Adaptive method of dim small object detection with heavy clutter

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APPLIED OPTICS 2013年第10期52卷 D64-D74页

作者： Meng, Wei Jin, Tao Zhao, Xinwei Zhejiang Univ Dept Informat Sci & Elect Engn Hangzhou 310027 Zhejiang Peoples R China

This paper investigates an adaptive method of dim small target detection in infrared images with a complex background. We analyze in depth the characteristics of the background, the target, and the noise in the gray intensity, space and frequency domain of the images. The modified top-hat transformation using interrelated structuring elements is adopted to adaptively detect the darker and the brighter targets and greatly suppress the cluttered background. Lateral pattern inhibition enhances the local contrast ratio and simultaneously identifies the targets of interest. The automatic threshold is used to enhance real dim targets in the cluttered background. A simulation based on the proposed algorithm is carried out and the results prove that the algorithm is effective and valid. (C) 2013 Optical Society of America

关键词： Genetic algorithms image processing image processing algorithms Joint transform correlators Object detection Signal processing

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Three-dimensional measuring technique for surface topography using a light-sectioning microscope

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APPLIED OPTICS 2012年第8期51卷 1162-1170页

作者： Xia, Linglin Chen, Peifeng Wang, Ying Zhou, Le Luo, Xi Huazhong Univ Sci & Technol Sch Optoelect Sci & Engn Wuhan Natl Lab Optoelect Wuhan 430074 Peoples R China Nanchang Univ Sch Software Nanchang 330029 Jiangxi Peoples R China

Three-dimensional (3D) surface topographic analysis, measurement, and assessment techniques have raised great interest not only among researchers but also among industrial users. Many industrial processes and applications are directly influenced by the small-scale roughness of surface finishes. This paper describes the development and implementation of a noncontact, three-dimensional, microtopography measuring system. The instrument is formed by combining a modified light-sectioning microscope subsystem with a computer subsystem. In particular, optical system characteristics of the light-sectioning microscope are investigated, and a textured steel sheet is measured to demonstrate good practical outcomes. Details of measuring processes and image processing algorithms are provided, such as procedures for measurement, image edge extraction, and 3D topography reconstruction. After the 3D topography of the measured surface has been reconstructed, the topography field description parameters are calculated. A standard roughness block was used for calibration of the surface microtopography measuring system. Results obtained showed the measurement method output has good agreement with the actual asperity (unevenness or roughness) of the surface. The computer subsystem is used to process and control asperity measurements and image generation, and for image acquisition and presentation. (C) 2012 Optical Society of America

关键词： image processing algorithms Light scattering Scanning electron microscopy Scanning tunneling microscopy Three dimensional reconstruction White light interferometry

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Measurement of wide frequency range structural microvibrations with a pocket digital camera and sub-pixel techniques

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APPLIED OPTICS 2012年第14期51卷 2664-2671页

作者： Mas, David Espinosa, Julian Roig, Ana B. Ferrer, Belen Perez, Jorge Illueca, Carlos Univ Alicante Inst Fis Aplicada Ciencias & Tecnol E-03080 Alicante Spain Univ Alicante Dep Ing Construcc E-03080 Alicante Spain

Analysis of vibrations and displacements is a hot topic in structural engineering. Although there is a wide variety of methods for vibration analysis, direct measurement of displacements in the mid and high frequency range is not well solved and accurate devices tend to be very expensive. Low-cost systems can be achieved by applying adequate image processing algorithms. In this paper, we propose the use of a commercial pocket digital camera, which is able to register more than 420 frames per second (fps) at low resolution, for accurate measuring of small vibrations and displacements. The method is based on tracking elliptical targets with sub-pixel accuracy. Our proposal is demonstrated at a 10 m distance with a spatial resolution of 0.15 mm. A practical application over a simple structure is given, and the main parameters of an attenuated movement of a steel column after an impulsive impact are determined with a spatial accuracy of 4 mu m. (C) 2012 Optical Society of America

关键词： Analytical techniques Frequency measurement High speed photography image processing image processing algorithms image quality

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