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Herb-partitioned moxibustion regulated the mirna expression profile in the thyroid tissues of rats with experimental autoimmune thyroiditis

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Journal of Traditional Chinese Medicine 2021年第5期41卷 789-798页

作者： WANG Zhaoqin CUI Yunhua HUANG Yan WU Huangan ZHAO Jimeng HE Jinsen LIU Huirong ZHENG Handan WU Luyi HU Zhihai LIU Yanan Department of Aeronautics and Astronautics Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint FunctionFudan UniversityShanghai 200433China Key Laboratory of Acupuncture and Immunological Effects Shanghai University of Traditional Chinese MedicineShanghai 200030China Yueyang Hospital of Integrative Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Shanghai TCM-integrated Hospital Shanghai University of Traditional Chinese MedicineShanghai 200082China

OBJECTIVE:To observe the effect of herb-partitioned moxibustion(HPM)on the mirna expression profile of thyroid tissue in experimental autoimmune thyroiditis(EAT)***:Rats were randomly divided into normal control(NC)group,EAT model(EAT)group,HPM group and western medicine(Med)*** model rats were prepared by a combined immunization with complete and incomplete Freund’s adjuvant emulsified with porcine thyroglobulin and *** in the HPM group were treated with HPM,while rats in the Med group were treated with levothyrocine(1μg/2 m L)by *** staining was used to observe the pathological morphological changes of thyroid tissue,ELISAs was uaed to detect the serum concentrations of TGAb,TPOAb,FT3,FT4,*** then performed high-throughput mi RNA sequencing to analyse the mi RNA expression profiles in the thyroid tissues,followed by a bioinformatics ***-q PCR was used to verify the identified differentially expressed mi ***:HPM improved the thyroid tissue morphology and reduced serum TPOAb,TGAb,TSH concentration in EAT rats(P<0.05),but with no obvious effect on FT3 and FT4 *** the TSH,FT3 and FT4 concentration was significantly changed in the Med group(P<0.01 or P<0.05)compared with that of EAT *** results showed that a total of 17 mi RNAs were upregulated,and 4 were downregulated in the EAT rats,in which the expression levels of mi R-346 and mi R-331-5 p were reversed by *** target genes of the mi RNAs that regulated by HPM wereassociated with a variety of immune factors and immune ***-q PCR verification showed that the expression of mi RNA-346 and mi RNA-331-5 p was consistent with the sequencing ***:HPM could regulate the the expression of mi RNA-346 and mi RNA-331-5 p,then act on their target genes to immune and inflammation-related pathways,which may be one of the mechanisms of HPM on EAT rats.

关键词： Experimental autoimmune thyroiditis Herb-partitioned moxibustion mirna expression profile Immuneregulation

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A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease

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PARASITOLOGY RESEARCH 2024年第10期123卷 1-15页

作者： Xiao, Jing Wu, Jian-Wen Xin, Yun-Zhuo Song, Dong Gao, Xiao-ping Yin, Mei Zhao, Wei Liu, Fu-lin Wang, Hao Wang, Jie Zhao, Jia-Qing Ningxia Med Univ Sch Basic Med Yinchuan Peoples R China Ningxia Med Univ Dept Otolaryngol Head & Neck Surg Gen Hosp Yinchuan Peoples R China Ningxia Med Univ Dept Resp Med Gen Hosp Yinchuan Peoples R China Ningxia Med Univ Sci Technol Ctr Yinchuan Peoples R China Ningxia Key Lab Prevent & Control Common Infect Di Yinchuan Peoples R China Shiyan Integrated Tradit Chinese & Western Med Hos Shiyan Peoples R China

Echinococcosis is a zoonotic disease, which seriously endangers human health. The immune game between parasite and host is not fully understood. Exosomes are thought to be one of the ways of information communication between parasite and host. In this study, we attempted to explore the communication between Echinococcus granulosus and its host through the medium of exosomes. We collected plasma from *** patients (CE-EXO) and healthy donors (HD-EXO) and extracted exosomes from the plasma. The expression profile of mirna in plasma was determined by second generation sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to annotate the function of target genes of differential mirnas. Meanwhile, we co-cultured plasma exosomes from healthy donors and plasma exosomes from *** patients with Jurkat T cells with or without phytohaemagglutinin (PHA) stimulation. The expression of CD69 on Jurkat T cells was detected by flow cytometry. The results showed that the mirna of exosomes between healthy donors and *** patients was significantly different. GO and KEGG were used to annotate the function of target genes of differential mirnas. The results indicate that many important pathways are involved in inflammation, metabolism, and immune response after parasite infection, such as p53 signaling pathway, PI3K-Akt signaling pathway, and glycolysis/gluconeogenesis. Flow cytometry showed that CE-EXO reduced the expression of CD69 + on Jurkat T cells. Our present results suggest that these differentially expressed mirnas may be important regulators of parasite-host interactions. Meanwhile, functional prediction of its target genes provides valuable information for understanding the mechanism of host-parasite interactions. These results provide clues for future studies on *** escape from host immune attack, which could help control *** infection.

关键词： Echinococcus granulosus mirna expression profile Exosome High-throughput sequencing Flow cytometry

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Marasmius androsaceus mitigates depression-exacerbated intestinal radiation injuries through reprogramming hippocampal mirna expression

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BIOMEDICINE & PHARMACOTHERAPY 2023年第1期165卷 115157-115157页

作者： Zhao, Jiamin Zeng, Xiaozhou Liu, Jia Liu, Xiaojing Liu, Zhihong Wang, Bin Chen, Zhiyuan Dong, Yanxi Guo, Suping Cui, Ming Xiao, Huiwen Liu, Xingzhong Nankai Univ State Key Lab Med Chem Biol Key Lab Mol Microbiol & Technol Minist EducDept MicrobiolColl Life Sci 94 Weijin Rd Tianjin 300071 Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Tianjin Key Lab Radiat Med & Mol Nucl Med Inst Radiat Med 238 Baidi Rd Tianjin 300192 Peoples R China Soochow Univ Dept Gen Surg Affiliated Hosp 2 Dushuhu Campus199 Ren Ai Rd Suzhou 215123 Peoples R China Shanxi Inst Med & Life Sci 61 Pingyang Rd Taiyuan 030012 Peoples R China Shanxi Kangxin Pharmaceut Co Ltd 1 West Ring Rd Luliang 030082 Peoples R China

Introduction: Cancer patients commonly experience high levels of psychological stress, which poses significant risks to their well-being. Radiotherapy is a primary treatment modality for cancer;however, it often leads to intestinal injuries in these patients. Nevertheless, the impact of mental stress on radiotherapy-intertwined complications remains ***: To induce intestinal injury, we employed total abdominal irradiation in our experimental model. We conducted high-throughput sequencing to analyze the expression profile of mirnas in the ***: We observed that mice with depression exhibited more severe intestinal injuries following total abdominal irradiation. Remarkably, oral administration of Marasmius androsaceus not only alleviated the depressive phenotype but also mitigated radiation-induced intestinal toxicity. Notably, this radioprotective effect was not observed in mice without depression. Depression disrupted the hippocampal mirna expression profile in mice subjected to local irradiation of the abdomen, leading to the accumulation of miR-139-5p and miR-184-3p in the hippocampus, serum, and small intestine tissues. However, treatment with Marasmius androsaceus reprogrammed the mirna expression signature in mice with depression. Furthermore, intravenous injection of antagomirs targeting miR-139-5p and miR-184-3p ameliorated depression, up-regulated Spn expression, reduced radiation enteritis, and improved the integrity of the small intestine in irradiated ***: Our findings demonstrate the efficacy of Marasmius androsaceus, a small mushroom, in alleviating depression-aggravated intestinal toxicity following radiotherapy by reprogramming hippocampal mirna expression.

关键词： Depression Hippocampus mirna expression profile Mushroom Radiation injury

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Comparison of mirna landscapes between the human oocytes with or without arrested development

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JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 2022年第10期39卷 2227-2237页

作者： Wei, Lina Yang, Xi Gao, Linzhi Liang, Zhongkun Yu, Hao Zhang, Ningfeng Li, Yi Jinan Univ Med Coll Div Histol & Embryol Int Joint Lab Embryon Dev & Prenatal Med Guangzhou Peoples R China Sun Yat Sen Univ Sun Yat Sen Mem Hosp Ctr Reprod Med Guangzhou Peoples R China Jinan Univ Affiliated Hosp 1 Dept Obstet & Gynecol Guangzhou Peoples R China Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Urol Guangzhou Peoples R China

Purpose By exploring the role of mirnas in human oocyte development, the study was conducted to investigate the epigenetic mechanism contributing to the arrest of oocyte development. Methods In total, 140 oocytes from 22 patients were collected in the developmentally arrested oocyte (DAO) group, whereas 420 oocytes from 164 patients were harvested in the control group. The pooled RNA was extracted from all 20 oocytes to establish a RNA library. The total RNA of every ten oocytes was extracted for qPCR validation of mirna candidates. Bioinformatic software was applied to explore the mirna candidates and their target genes. Results Generally, the expression levels of mirnas altered slightly during normal oocyte development but changed dramatically in the DAOs. Among the top 10 differential mirnas, let-7a-5p and let-7g-5p, which were abundantly expressed throughout the oocyte development stages, had the broadest biological impact on oogenesis. Validated by qRT-PCR, both mirnas were profoundly suppressed in the DAOs. During normal oocyte development, the expression levels of let-7a-5p and let-7g-5p at the GV stage were significantly higher than at MI and MII stages. Bioinformatic analysis demonstrated that let-7a-5p and let-7g-5p might regulate oocyte development by targeting PI3K-Akt, P53, cell cycle, and FoxO signaling pathways. Conclusions There are dramatic differences in mirna landscapes between the human oocytes with or without development arrest. In addition, the suppression of let-7a-5p and let-7g-5p might be associated with the occurrence of development arrest. The findings could provide therapeutic targets to correct the arrest of oocyte development in the future.

关键词： mirna expression profile let-7 family member Oocyte maturation Oocyte development arrest

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Hypoxia treatment and resistance training alters microRNA profiling in rats skeletal muscle

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SCIENTIFIC REPORTS 2024年第1期14卷 1-13页

作者： Mei, Tao Hu, Yang Zhang, Ying Li, Yanchun Beijing Sport Univ China Inst Sport & Hlth Sci Beijing Peoples R China

MicroRNAs (mirnas) may play a crucial regulatory role in the process of muscle atrophy induced by high-altitude hypoxia and its amelioration through resistance training. However, research in this aspect is still lacking. Therefore, this study aimed to employ mirna microarray analysis to investigate the expression profile of mirnas in skeletal muscle from an animal model of hypoxia-induced muscle atrophy and resistance training aimed at mitigating muscle atrophy. The study utilized a simulated hypoxic environment (oxygen concentration at 11.2%) to induce muscle atrophy and established a rat model of resistance training using ladder climbing, with a total intervention period of 4 weeks. The mirna expression profile revealed 9 differentially expressed mirnas influenced by hypoxia (e.g., miR-341, miR-32-5p, miR-465-5p) and 14 differentially expressed mirnas influenced by resistance training under hypoxic conditions (e.g., miR-338-5p, miR-203a-3p, miR-92b-3p) (divided by log2(FC)divided by >= 1.5, p < 0.05). The differentially expressed mirnas were found to target genes involved in muscle protein synthesis and degradation (such as Utrn, mdm2, eIF4E), biological processes (such as negative regulation of transcription from RNA polymerase II promoter, regulation of transcription, DNA-dependent), and signaling pathways (such as Wnt signaling pathway, MAPK signaling pathway, ubiquitin-mediated proteolysis, mTOR signaling pathway). This study provides a foundation for understanding and further exploring the molecular mechanisms underlying hypoxia-induced rats muscle atrophy and the mitigation of atrophy through resistance training.

关键词： Hypoxia Resistance training Skeletal muscle atrophy mirna expression profile Bioinformatics analysis

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Explore the Effect and Target of Liraglutide on Islet Function in Type 2 Diabetic Rats by mirna Omics Technology

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DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 2021年 14卷 3795-3807页

作者： Guo, Qiuyue Xu, Yunsheng Li, Jie An, Wenrong Luo, Dan Huang, Chengcheng Huang, Yanqin Shanghai Univ Tradit Chinese Med Dept Endocrinol Shuguang Hosp Shanghai 201203 Peoples R China Shandong Univ Tradit Chine Dept Endocrinol Affiliated Hosp 2 Jinan 250001 Peoples R China Shandong Univ Tradit Chinese Med Clin Med Coll 1 Jingshi Rd Campus Jinan 250014 Peoples R China Shandong Univ Tradit Chinese Med Dept Endocrinol Affiliated Hosp Jinan 250014 Peoples R China

Purpose: To analyze the effect and potential therapeutic targets of liraglutide in type 2 diabetes through mirna expression profiling Methods: Ten of 30 SPF Wistar rats, males at 4 weeks old, were randomly selected as the control group and given conventional feed, the other rats adopted high-sugar and high-fat diet combined with an intraperitoneal injection of streptozotocin to establish a T2DM model. One unsuccessful rat was excluded, and the remaining rats were randomized to the model and the liraglutide group. Liraglutide group was subcutaneously injected with liraglutide 0.11 mg/kg for 8 weeks. The biochemical indicators and staining HE were detected. The expression of mirna in pancreatic tissue was detected by mirna sequencing. The intersection of mirna difference was used to predict the target gene, then functional enrichment was performed to identify its possible biological functions and signal transduction paths. Finally, qRT-PCR was used to verify the results. Results: Compared to the model group, the level of fasting blood glucose (FBG), glucagon and insulin resistance index (HOMA-IR) in the liraglutide group were significantly decreased, fasting insulin (FINS) and insulin sensitivity index (ISI) were increased. Nine differential mirnas (miR-135a-5p, miR-144-5p, miR-21-3p, miR-215, miR-451-5p, miR-486, miR-122-5p, miR-181d-5p and miR-345-5p) were identified at the intersection through two mirna sequencing. A total of 3359 related target gene predictions were obtained. GO and pathway analyses demonstrated that differentially expressed genes were closely related to cell proliferation, angiogenesis, and proteolysis. Significant signaling pathways included PI signaling system, autophagy, FoxO and HIF-1 signaling pathway. Conclusion: Liraglutide could improve islet function by regulating nine mirnas, and the related signaling pathways included PI signaling system, autophagy, FoxO and HIF-1 signaling pathway. Our study provided the basis and direction for furthe

关键词： liraglutide type 2 diabetes mirna expression profile target gene signaling pathway

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Identification of key mirna signature and pathways involved in multiple myeloma by integrated bioinformatics analysis

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HEMATOLOGY 2021年第1期26卷 976-984页

作者： Dong, Xiushuai Lu, Gang Su, Xianwei Liu, Jie Chen, Xi Tian, Yaoyao Chang, Yuying Wang, Lianjie Wang, Wei Zhou, Jin Harbin Med Univ Affiliated Hosp 1 Dept Hematol Harbin Peoples R China Harbin Med Univ Affiliated Hosp 2 Dept Hematol Harbin Peoples R China Chinese Univ Hong Kong Sch Biomed Sci CUHK SDU Joint Lab Reprod Genet Hong Kong Peoples R China

Objectives: Multiple myeloma (MM) is one of the most common types of hematologic malignancy for which the underlying molecular mechanisms remain largely unclear. Dysregulated mirna expression has been shown to be involved in MM tumorigenesis, progression and drug response. Therefore, a comprehensive analysis based on mirna-level integrated strategy was performed. Methods: This study aimed to elucidate key mirna signatures and pathways in MM by integrated bioinformatics analysis. expression profiles GSE24371, GSE49261 and GSE54156 were obtained from the Gene expression Omnibus database, and differentially expressed mirnas (DEMirs) with p < 0.05 were identified. The target genes of these DEMirs were obtained from ENCORI database, and functional enrichment, subpathway enrichment and protein-protein interaction network construction were performed. The key target genes were identified by random walk algorithm and survival verification was performed. Results and discussion: First, six up-regulated and four down-regulated DEMirs shared between any two GSE data sets were identified. Second, target genes (DEMirTGs) by up-regulated and down-regulated DEMirs were obtained. Functional and subpathway enrichment analysis showed that these up-regulated DEMirs are consistently involved in the Wnt signaling pathway. Moreover, enrichment of the down-regulated DEMirs is mainly in the MAPK signaling pathway. Finally, a protein-protein interaction sub-network for these DEMirTGs was constructed, the correlations between the two key genes were identified and survival in MM was evaluated using multiple independent data sets. Conclusion: We identified mirna signatures and key target genes that were closely related to MM biology, and these genes might serve as potential therapeutic targets for MM patients.

关键词： Multiple myeloma mirna expression profile network analysis random walk algorithm

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Identification and profiles of microRNAs in different development stages of miniature pig secondary palate

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GENOMICS 2021年第4期113卷 2634-2644页

作者： Xu, Ying Yuan, Dong Fan, Zhipeng Wang, Songlin Du, Juan Capital Med Univ Sch Stomatol Dept Geriatr Dent Tiantan Xili 4 Beijing 100050 Peoples R China Capital Med Univ Sch Stomatol Lab Mol Signaling & Stem Cells Therapy Mol Lab Gene Therapy & Tooth RegeneratBeijing Ke Tiantan Xili 4 Beijing 100050 Peoples R China Capital Med Univ Sch Basic Med Sci Dept Biochem & Mol Biol You An Men Wai Xi Tou Tiao 10 Beijing 100069 Peoples R China

Cleft palate is one of the most frequent craniofacial malformation birth defects. Miniature pigs (Sus scrofa) are a valuable alternative large animal model to explore human palate development. Presently, the microRNA (mirna) expression profiles in miniature pigs during palatogenesis from embryonic day (E) 30 to 50 were identified. A total of 2044 known mirnas and 192 novel mirnas were identified. The functional characteristics of their potential target genes were identified using Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway analysis. mirnas displayed diverse expression levels among the different stages. Using Short Time-series expression Miner software to investigate the expression patterns of mirnas from E30-50, all mirnas were clustered into 20 profiles. The profiles showing mirnas expression decreased (profile 0)/increased (profile 19) from E30-50 were the main patterns during palatogenesis. Hub genes of four significant modules were identified by weighted correlation network analysis, including ssc-miR-98, ssc-miR-27a_R + 1, and ssc-miR150, etc. which might be novel potential targets for regulating palate development. The data are expected to improve the understanding of palate development and the etiology of cleft palate in further studies.

关键词： mirna expression profile Miniature pig Palate development Cleft palate (CP)

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Possible microRNA-based mechanism underlying relationship between chronic spontaneous urticaria and Blastocystis

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EXPERIMENTAL PARASITOLOGY 2023年第1期245卷 108453页

作者： Orsten, Serra Baysal, Ipek Akdogan, Neslihan Inal, Nese Bostan, Ecem Ciftci, Samiye Yabanoglu Akyon, Yakut Hacettepe Univ Vocat Sch Hlth Serv Adnan Saygun StD Blocks TR-06100 Ankara Turkey Hacettepe Univ Fac Med Dept Dermatol & Venereol Ankara Turkey Hacettepe Univ Fac Med Dept Med Microbiol Ankara Turkey Hacettepe Univ Fac Pharm Dept Biochem Ankara Turkey

Background: Blastocystis spp. has been proposed as a possible cause of extraintestinal clinical signs such as urticaria pathogenesis. Objectives: The aim of this study was to investigate the differences between microRNA (mirna) expression profiles of Chronic spontaneous urticaria (CSU) patients in the presence or absence of Blastocystis spp. as well as healthy controls. Additionally, cellular pathways which are affected in the presence of Blastocystis spp. were identified. Methods: Twenty patients diagnosed with CSU were enrolled in the study and divided into equally two groups according to the presence of Blastocystis spp. Besides, six healthy individuals were included in the study. The expression profiles of 372 human-derived mirnas have been investigated in serum samples from CSU patients and healthy controls with miScript mirna PCR Array Human miRBase ***: Compared to Blastocystis-negative (BN)-CSU patients, expression of 3 mirnas (hsa-miR-3183, hsa-miR4469, hsa-miR-5191) were found to be downregulated by at least two-fold (p < 0.05) in Blastocystis-positive (BP)CSU patients. Additionally, the mirna expression profiles of six healthy individuals (n = 3 Blastocystis-positive, n = 3 Blastocystis-negative) were analyzed and it was determined that the expressions of 7 mirnas (hsa-miR4661-5p, hsa-miR-4666a-5p, hsa-miR-4803, hsa-miR-5587-5p, hsa-miR-4500, hsa-miR-5680, hsa-miR-382-3p) increased at least 3-fold in the serum of individuals with Blastocystis-positive compared to Blastocystis-negative subjects. Most down-regulated mirnas, in BP-CSU patients, affect cell adhesion molecules (CAMs), and signaling pathways therefore, Blastocystis spp. presence may influence the clinical presentation of urticaria by leading to unbalanced immunity. In addition, Blastocystis spp. presence may be influenced TGF- beta signaling pathway through altered mirnas and may be laying the groundwork for the development of CSU in healthy individuals. Conclusions: As a consequen

关键词： Chronic spontaneous urticaria (CSU) Blastocystis microRNA mirna expression profile CCL17 TGF-?

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Analysis of mirna expression profiles in melatonin-exposed GC-1 spg cell line

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GENE 2018年 642卷 513-521页

作者： Zhu, Xiaoling Chen, Shuxiong Jiang, Yanwen Xu, Ying Zhao, Yun Chen, Lu Li, Chunjin Zhou, Xu Jilin Univ Jilin Prov Key Lab Anim Embryo Engn Coll Anim Sci 5333 Xian Rd Changchun 130062 Jilin Peoples R China Jilin Univ Hosp 2 Reprod Med Ctr Changchun 130062 Jilin Peoples R China

Melatonin is an endocrine neurohormone secreted by pinealocytes in the pineal gland. It exerts diverse physiological effects, such as circadian rhythm regulator and antioxidant. However, the functional importance of melatonin in spermatogenesis regulation remains unclear. The objectives of this study are to: (1) detect melatonin affection on mirna expression profiles in GC-1 spg cells by mirna deep sequencing (DeepSeq) and (2) define melatonin affected mirna mRNA interactions and associated biological processes using bioinformatics analysis. GC-1 spg cells were cultured with melatonin (10(-7) M) for 24 h. DeepSeq data were validated using quantitative real-time reverse transcription polymerase chain reaction analysis (qRT-PCR). A total of 176 mirna expressions were found to be significantly different between two groups (fold change of > 2 or < 0.5 and FDR < 0.05). Among these expressions, 171 were up-regulated, and 5 were down-regulated. Ontology analysis of biological processes of these targets indicated a variety of biological functions. Pathway analysis indicated that the predicted targets were involved in cancers, apoptosis and signaling pathways, such as VEGF, TNF, Ras and Notch. Results implicated that melatonin could regulate the expression of mirna to perform its physiological effects in GC-1 spg cells. These results should be useful to investigate the biological function of mirnas regulated by melatonin in spermatogenesis and testicular germ cell tumor.

关键词： GC-1 spg cell line Melatonin mirna expression profile

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