Knelangen JM, van der Hoek MB, Kong WC, Owens JA, Fischer B, Navarrete Santos A. microrna expression profile during adipogenic differentiation in mouse embryonic stem cells. Physiol Genomics 43: 611-620, 2011. First p...
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Knelangen JM, van der Hoek MB, Kong WC, Owens JA, Fischer B, Navarrete Santos A. microrna expression profile during adipogenic differentiation in mouse embryonic stem cells. Physiol Genomics 43: 611-620, 2011. First published January 18, 2011;doi:10.1152/physiolgenomics.00116.2010.-Pluripotent embryonic stem cells (ESC) have the potential to differentiate into any cell type of the three germ layers. Differentiation processes depend on genetic and epigenetic factors. The guidance of cell fate determination by micrornas (miRs) seems important for embryonic development and cell lineage decisions. MiRs are short, single-stranded, noncoding RNA molecules that regulate through posttranscriptional modulation, a subset of target genes involved in cell differentiation and specific cell function. We have used microarray profiling of miRs in the mouse embryonic stem cell line CGR8. Comparison of the miR profiles of undifferentiated stem cells with mesodermal progenitors cells (day 5), preadipocytes (day 10), and adipocytes (day 21) showed that the expression level of 129 miRs changed (twofold) during adipogenic differentiation. We identified 10 clusters of differentially expressed miRs, which contain putative markers and regulators of mesodermal differentiation and cell fate determination into adipocytes. Notably, the adipocyte-specific miRs 143 and 103 were upregulated from day 10 onward. We have therefore demonstrated and characterized the dynamic profile of miR expression during murine adipogenic differentiation in vitro, including the initial differentiation from ESC via mesenchymal progenitors up to adipocytes. Our findings and experimental approach provide a suitable system to directly interrogate the role of miRs during adipogenic differentiation of embryonic stem cells.
micrornas (miRNAs) are posttranscriptional modulators of gene expression that play important roles in various biological processes. Spermatogenesis is a highly regulated process in which diploid spermatogonia eventual...
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micrornas (miRNAs) are posttranscriptional modulators of gene expression that play important roles in various biological processes. Spermatogenesis is a highly regulated process in which diploid spermatogonia eventually differentiate into haploid spermatozoa. In this study, we identified four differentially expressed miRNAs between two premeiotic male germ cells, made predictions about their putative targets, and confirmed cyclin T2 (Ccnt2) as a direct target of miR-15a. We also report that miR-15a inhibited muscle differentiation at least in part by targeting Ccnt2, which represents a novel interaction. Subsequently, miR-15a and Ccnt2 were profiled in developing mice testes to observe their inverse correlations in the postnatal 3-week period to understand their roles in spermatogenesis. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip *** hsa-miRNA...
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The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip *** hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was *** on the bioinformatic approach,chip data was analyzed to select out differentially expressed candidate ***-time quantitative PCR(RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all *** results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal *** four uveal melanomas,expressions of miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,and miRNA-34a were significantly up-regulated,while miRNA-145 and miRNA-204 expression were significantly *** used miRNA microarray analysis as a fast,efficient technology to study biological *** differentially expressed miRNAs may be involved in uveal melanoma pathogenesis,and may help promote the diagnosis and treatment for uveal melanoma.
Background: We hypothesised that differences in microrna expression profiles contribute to the contrasting natural history and clinical outcome of the two most common types of malignant germ cell tumour (GCT), yolk sa...
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Background: We hypothesised that differences in microrna expression profiles contribute to the contrasting natural history and clinical outcome of the two most common types of malignant germ cell tumour (GCT), yolk sac tumours (YSTs) and germinomas. Results: By direct comparison, using microarray data for paediatric GCT samples and published qRT-PCR data for adult samples, we identified micrornas significantly up-regulated in YSTs (n = 29 paediatric, 26 adult, 11 overlapping) or germinomas (n = 37 paediatric). By Taqman qRT-PCR we confirmed differential expression of 15 of 16 selected micrornas and further validated six of these (miR-302b, miR-375, miR-200b, miR-200c, miR-122, miR-205) in an independent sample set. Interestingly, the miR-302 cluster, which is over-expressed in all malignant GCTs, showed further over-expression in YSTs versus germinomas, representing six of the top eight micrornas over-expressed in paediatric YSTs and seven of the top 11 in adult YSTs. To explain this observation, we used mRNA expression profiles of paediatric and adult malignant GCTs to identify 10 transcription factors (TFs) consistently over-expressed in YSTs versus germinomas, followed by linear regression to confirm associations between TF and miR-302 cluster expression levels. Using the sequence motif analysis environment iMotifs, we identified predicted binding sites for four of the 10 TFs (GATA6, GATA3, TCF7L2 and MAF) in the miR-302 cluster promoter region. Finally, we showed that miR-302 family over-expression in YST is likely to be functionally significant, as mRNAs down-regulated in YSTs were enriched for 3' untranslated region sequences complementary to the common seed of miR-302a similar to miR-302d. Such mRNAs included mediators of key cancer-associated processes, including tumour suppressor genes, apoptosis regulators and TFs. Conclusions: Differential microrna expression is likely to contribute to the relatively aggressive behaviour of YSTs and may enable future impr
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